Jump to content

Beta thymosins: Difference between revisions

From Wikipedia, the free encyclopedia
Browse history interactively
← Previous edit
Content deleted Content added
VisualWikitext
Jgedwards (talk | contribs)
285 edits
OAbot (talk | contribs)
Bots
438,441 edits
m Open access bot: doi updated in citation with #oabot.
 
(45 intermediate revisions by 19 users not shown)
Line 1: Line 1:
[[Image:Thymosin3.gif|thumb|alt=alt text| [[NMR]] structure of a β-thymosin. Both thymosin α<sub>1</sub> <ref>{{cite journal |author=Grottesi A, Sette M, Palamara T, Rotilio G, Garaci E, Paci M |title=The conformation of peptide thymosin alpha 1 in solution and in a membrane-like environment by circular dichroism and NMR spectroscopy. A possible model for its interaction with the lymphocyte membrane |journal=Peptides |volume=19 |issue=10 |pages=1731–8 |year=1998 |pmid=9880079 |doi= 10.1016/S0196-9781(98)00132-6|url=}}</ref> and β-thymosins are [[intrinsically unstructured proteins]], i.e. they lack a stable fold when free in aqueous solution. This structure, mostly [[alpha helix]], was artificially stabilised by an organic solvent.<ref name="1HJ0.pdb"/> The thymosin illustrated, originally named β<sub>9</sub> is the cow [[orthologue]] of human β<sub>10</sub> ]]
[[Image:Thymosin3.gif|thumb|alt=alt text| [[NMR]] structure of a β-thymosin. Both thymosin α<sub>1</sub> <ref>{{cite journal |vauthors=Grottesi A, Sette M, Palamara T, Rotilio G, Garaci E, Paci M |title=The conformation of peptide thymosin alpha 1 in solution and in a membrane-like environment by circular dichroism and NMR spectroscopy. A possible model for its interaction with the lymphocyte membrane |journal=Peptides |volume=19 |issue=10 |pages=1731–8 |year=1998 |pmid=9880079 |doi= 10.1016/S0196-9781(98)00132-6|s2cid=39655090 }}</ref> and β-thymosins are [[intrinsically unstructured proteins]], i.e. they lack a stable fold when free in aqueous solution. This structure, mostly [[alpha helix]], was artificially stabilised by an organic solvent.<ref name="1HJ0.pdb">{{PDB|1HJ0}}; {{cite journal | vauthors = Stoll R, Voelter W, Holak TA | title = Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy | journal = Biopolymers | volume = 41 | issue = 6 | pages = 623–34 | date = May 1997 | pmid = 9108730 | doi = 10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S | quote = The thymosin is β9, bovine orthologue of human β10. Stabilised by organic solvent, the structure was determined by NMR. (Free β-thymosins lack a stable fold in solution) | doi-access = free }}</ref> The thymosin illustrated, originally named β<sub>9</sub> is the cow [[orthologue]] of human β<sub>10</sub> ]]
'''Beta thymosins''' are a family of proteins which have in common a sequence of about 40 amino acids similar to the small protein thymosin β<sub>4</sub>. They are found almost exclusively in multicellular animals. Thymosin β<sub>4</sub> was originally obtained from the [[thymus]] in company with several other small proteins which although named collectively "[[thymosins]]" are now known to be structurally and genetically unrelated and present in many different animal tissues.
'''Beta thymosins''' are a family of proteins which have in common a sequence of about 40 amino acids similar to the small protein [[Thymosin β4|thymosin β<sub>4</sub>]]. They are found almost exclusively in multicellular animals. Thymosin β<sub>4</sub> was originally obtained from the [[thymus]] in company with several other small proteins which although named collectively "[[thymosins]]" are now known to be structurally and genetically unrelated and present in many different animal tissues.

== See also==
⚫
* [[Thymosins]]
⚫
* [[Thymosin α1]]


== Single domain β-thymosins ==
== Single domain β-thymosins ==
Line 14: Line 10:
| image = PDB 1hj0 EBI.jpg
| image = PDB 1hj0 EBI.jpg
| width =
| width =
| caption = Structure of thymosin beta 9.<ref name="pmid9108730">{{cite journal |author=Stoll R, Voelter W, Holak TA |title=Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy |journal=Biopolymers |volume=41 |issue=6 |pages=623–34 |year=1997 |month=May |pmid=9108730 |doi=10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S |url=}}</ref>
| caption = Structure of thymosin beta 9.<ref name="pmid9108730">{{cite journal |vauthors=Stoll R, Voelter W, Holak TA |title=Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy |journal=Biopolymers |volume=41 |issue=6 |pages=623–34 |date=May 1997 |pmid=9108730 |doi=10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S |doi-access=free }}</ref>
| Pfam = PF01290
| Pfam = PF01290
| InterPro = IPR001152
| InterPro = IPR001152
Line 25: Line 21:
| PDB = {{PDB2|1hj0}}, {{PDB2|1sqk}}, {{PDB2|1t44}}, {{PDB2|2ff6}}
| PDB = {{PDB2|1hj0}}, {{PDB2|1sqk}}, {{PDB2|1t44}}, {{PDB2|2ff6}}
}}
}}
[[Monomeric]] β-thymosins, i.e. those of molecular weight similar to those originally isolated from thymus by Goldstein, are found almost exclusively in cells of multicellular animals.<ref name="url_ PF01290">{{cite web | url = http://pfam.sanger.ac.uk/family?acc=PF01290#tabview=tab1 | title = Family: Thymosin (PF01290) | author = | authorlink = | coauthors = | date = | format = | work = Pfam | publisher = Wellcome Trust Sanger Institute | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = }}</ref> Known exceptions are significantly monomeric thymosins found in a few single-celled organisms presently regarded as their closest single-celled relatives <ref name="pmid18461162">{{cite journal |author=Shalchian-Tabrizi K, Minge MA, Espelund M, Orr R, Ruden T, Jakobsen KS, Cavalier-Smith T |title=Multigene phylogeny of choanozoa and the origin of animals |journal=Plos One |volume=3 |issue=5 |pages=e2098 |year=2008 |pmid=18461162 |pmc=2346548 |doi=10.1371/journal.pone.0002098 |url=}}</ref>, choanoflagellates <ref>{{cite web |url=http://www.ncbi.nlm.nih.gov/nucest/170627636?rid=z9eskab4016&blast_rank=1&log$=nuclalign&report=genbank |title=XYM2758.rev XYM Monosiga brevicollis rapidly growi... - EST result |format= |work= |accessdate=}}
[[Monomeric]] β-thymosins, i.e. those of molecular weight similar to the peptides originally isolated from thymus by Goldstein, are found almost exclusively in cells of multicellular animals.<ref name="url_ PF01290">{{cite web | url = http://pfam.sanger.ac.uk/family?acc=PF01290#tabview=tab1 | title = Family: Thymosin (PF01290) | work = Pfam | publisher = Wellcome Trust Sanger Institute | archive-url = https://web.archive.org/web/20080126210317/http://pfam.sanger.ac.uk/family?acc=PF01290#tabview=tab1 | archive-date = 2008-01-26 | url-status = dead }}</ref> Known exceptions are monomeric thymosins found in a few single-celled organisms, significantly those currently regarded as the closest relatives of multicellular animals:<ref name="pmid18461162">{{cite journal | vauthors = Shalchian-Tabrizi K, Minge MA, Espelund M, Orr R, Ruden T, Jakobsen KS, Cavalier-Smith T | title = Multigene phylogeny of choanozoa and the origin of animals | journal = PLOS ONE | volume = 3 | issue = 5 | pages = e2098 | year = 2008 | pmid = 18461162 | pmc = 2346548 | doi = 10.1371/journal.pone.0002098 | bibcode = 2008PLoSO...3.2098S | doi-access = free }}</ref> choanoflagellates <ref>{{cite journal |url=https://www.ncbi.nlm.nih.gov/nucest/170627636?rid=z9eskab4016&blast_rank=1&log$=nuclalign&report=genbank |title=XYM2758.rev XYM Monosiga brevicollis rapidly growi... - EST result |date=2008-03-20 }}
</ref> and filastereans. Although found in very early diverged animals such as sponges, monomeric thymosins are absent from arthropods and nematodes, which do nevertheless possess proteins, known as β-thymosin repeat proteins, which are constructed from several end-to-end repeats of β-thymosin sequences.<ref name="pmid11040289">{{cite journal |author=Manuel M, Kruse M, Müller WE, Le Parco Y |title=The comparison of beta-thymosin homologues among metazoa supports an arthropod-nematode clade |journal=J. Mol. Evol. |volume=51 |issue=4 |pages=378–81 |year=2000 |month=October |pmid=11040289 |doi= 10.1007/s002390010100|url=}}</ref> [[Genomics]] has shown that [[tetrapods]] (land vertebrates) each express three monomeric β-thymosins, which are the animal species' equivalents (orthologues) of human β<sub>4</sub>, β<sub>10</sub> and β<sub>15</sub> thymosins, respectively. The human thymosins are encoded by the genes [[thymosin beta-4|TMSB4X]], [[TMSB10]] and [[TMSB15A]] and [[TMSB15B]]. (In humans, the proteins encoded by the two TMSB15 genes are identical.) [[Bony fish]] in general express orthologues of these same three, plus an additional copy of the β<sub>4</sub> orthologue.<ref name="pmid20138884">{{cite journal | author = Edwards J | title = Vertebrate beta-thymosins: conserved synteny reveals the relationship between those of bony fish and of land vertebrates | journal = FEBS Lett. | volume = 584 | issue = 5 | pages = 1047–53 | year = 2010 | month = March | pmid = 20138884 | doi = 10.1016/j.febslet.2010.02.004 | url = | issn = }}</ref>
</ref> and filastereans.<ref>{{cite journal |url=https://www.ncbi.nlm.nih.gov/nucest/110050367 |title=NUE00005552 Capsaspora owczarzaki Amplicon express Capsaspora owczarza - EST - NCBI |date=2008-11-20 }}.</ref> Although found in very early-diverged animals such as sponges, monomeric thymosins are absent from arthropods and nematodes, which do nevertheless possess "β-thymosin repeat proteins" which are constructed from several end-to-end repeats of β-thymosin sequences.<ref name="pmid11040289">{{cite journal | vauthors = Manuel M, Kruse M, Müller WE, Le Parco Y | title = The comparison of beta-thymosin homologues among metazoa supports an arthropod-nematode clade | journal = J. Mol. Evol. | volume = 51 | issue = 4 | pages = 378–81 | date = October 2000 | pmid = 11040289 | doi = 10.1007/s002390010100 | bibcode = 2000JMolE..51..378M | s2cid = 8675540 }}</ref> [[Genomics]] has shown that [[tetrapods]] (land vertebrates) each express three monomeric β-thymosins, which are the animal species' equivalents (orthologues) of human β<sub>4</sub>, β<sub>10</sub> and β<sub>15</sub> thymosins, respectively. The human thymosins are encoded by the genes [[thymosin beta-4|TMSB4X]], [[TMSB10]] and [[TMSB15A]] and [[TMSB15B]]. (In humans, the proteins encoded by the two TMSB15 genes are identical.) [[Bony fish]] in general express orthologues of these same three, plus an additional copy of the β<sub>4</sub> orthologue.<ref name="pmid20138884">{{cite journal | vauthors = Edwards J | title = Vertebrate beta-thymosins: conserved synteny reveals the relationship between those of bony fish and of land vertebrates | journal = FEBS Lett. | volume = 584 | issue = 5 | pages = 1047–53 | date = March 2010 | pmid = 20138884 | doi = 10.1016/j.febslet.2010.02.004 | doi-access = free }}</ref>


{| class="wikitable"
{| class="wikitable"
Line 37: Line 33:
| rowspan="2" align="center" | β<sub>4</sub>
| rowspan="2" align="center" | β<sub>4</sub>
| [[Thymosin beta-4|TMSB4X]]
| [[Thymosin beta-4|TMSB4X]]
| [[X chromosome|Chr. X]] [http://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=Xq21.3 q21.3-q22]
| [[X chromosome|Chr. X]] [https://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=Xq21.3 q21.3-q22]
| Thymosin β<sub>4</sub>
| Thymosin β<sub>4</sub>
|-
|-
Line 46: Line 42:
| align="center" | β<sub>10</sub>
| align="center" | β<sub>10</sub>
| [[TMSB10]]
| [[TMSB10]]
| [[Chromosome 2 (human)|Chr. 2]] [http://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=2p11.2 p11.2]
| [[Chromosome 2 (human)|Chr. 2]] [https://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=2p11.2 p11.2]
| Thymosin β<sub>10</sub>
| Thymosin β<sub>10</sub>
|-
|-
| rowspan="2" align="center" | β<sub>15</sub>
| rowspan="2" align="center" | β<sub>15</sub>
| [[TMSB15A]]
| [[TMSB15A]]
| [[X chromosome|Chr. X]] [http://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=Xq21.33 q21.33-q22.3]
| [[X chromosome|Chr. X]] [https://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=Xq21.33 q21.33-q22.3]
| Thymosin β<sub>15</sub>
| Thymosin β<sub>15</sub>
|-
|-
| [[TMSB15B]]
| [[TMSB15B]]
| [[X chromosome|Chr. X]] [http://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=Xq22.2 q22.2]
| [[X chromosome|Chr. X]] [https://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?chromosome=Xq22.2 q22.2]
| Thymosin β<sub>15</sub>
| Thymosin β<sub>15</sub>
|}
|}

Thymosin β<sub>1</sub> was found to be [[ubiquitin]] (truncated by two C-terminal glycine residues).<ref name="pmid17468232"/>


=== Relation to the WH<sub>2</sub> sequence module ===
=== Relation to the WH<sub>2</sub> sequence module ===


The N-terminal half of β-thymosins bears a strong similarity in [[amino acid sequence]] to a very widely distributed sequence module, the [[WH2 motif|WH<sub>2</sub> module]]. (Wasp Homology Domain 2 - the name is derived from [[Wiskott-Aldrich syndrome protein]]).<ref name="pmid11911886">{{cite journal | author = Paunola E, Mattila PK, Lappalainen P | title = WH2 domain: a small, versatile adapter for actin monomers | journal = FEBS Lett. | volume = 513 | issue = 1 | pages = 92–7 | year = 2002 | month = February | pmid = 11911886 | doi = 10.1016/S0014-5793(01)03242-2| url = | issn = }}</ref><ref name="url_PF02205_tab6">{{cite web | url = http://pfam.sanger.ac.uk/family?acc=PF02205#tabview=tab6 | title = Family: WH2 (PF02205) | author = | authorlink = | coauthors = | date = | format = | work = Pfam | publisher = Wellcome Trust Sanger Institute | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = }}</ref> Evidence from [[X-ray crystallography]] shows that this part of β-thymosins binds to [[actin]] in a near-identical manner to that of WH<sub>2</sub> modules, both adopting as they bind, a conformation which has been referred to as the β-thymosin/WH<sub>2</sub> fold. β-thymosins may therefore have evolved by addition of novel C-terminal sequence to an ancestral WH<sub>2</sub> module.<ref name="pmid17468236">{{cite journal | author = Dominguez R | title = The beta-thymosin/WH2 fold: multifunctionality and structure | journal = Ann. N. Y. Acad. Sci. | volume = 1112 | issue = | pages = 86–94 | year = 2007 | month = September | pmid = 17468236 | doi = 10.1196/annals.1415.011 | url = | issn = }}</ref> However, sequence similarity searches designed to identify present-day WH2 domains<ref name="url_PF02205_tab2">{{cite web | url = http://pfam.sanger.ac.uk/family?acc=PF02205#tabview=tab2 | title = Family: WH2 (PF02205) | author = | authorlink = | coauthors = | date = | format = | work = Pfam | publisher = Wellcome Trust Sanger Institute | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = }}</ref> fail to recognise β-thymosins, (and ''vice versa'') and the sequence and functional similarities may result from [[convergent evolution]].<ref name="pmid15328003">{{cite journal | author = Edwards J | title = Are beta-thymosins WH2 domains? | journal = FEBS Lett. | volume = 573 | issue = 1–3 | pages = 231–2; author reply 233 | year = 2004 | month = August | pmid = 15328003 | doi = 10.1016/j.febslet.2004.07.038 | url = | issn = }}</ref>
The N-terminal half of β-thymosins bears a strong similarity in [[amino acid sequence]] to a very widely distributed sequence module, the [[WH2 motif|WH<sub>2</sub> module]]. (Wasp Homology Domain 2 - the name is derived from [[Wiskott-Aldrich syndrome protein]]).<ref name="pmid11911886">{{cite journal | vauthors = Paunola E, Mattila PK, Lappalainen P | title = WH2 domain: a small, versatile adapter for actin monomers | journal = FEBS Lett. | volume = 513 | issue = 1 | pages = 92–7 | date = February 2002 | pmid = 11911886 | doi = 10.1016/S0014-5793(01)03242-2 | doi-access = free }}</ref><ref name="url_PF02205_tab6">{{cite web | url = http://pfam.sanger.ac.uk/family?acc=PF02205#tabview=tab6 | title = Family: WH2 (PF02205) | work = Pfam | publisher = Wellcome Trust Sanger Institute }}{{Dead link|date=June 2020 |bot=InternetArchiveBot |fix-attempted=yes }}</ref> Evidence from [[X-ray crystallography]] shows that this part of β-thymosins binds to [[actin]] in a near-identical manner to that of WH<sub>2</sub> modules, both adopting as they bind, a conformation which has been referred to as the β-thymosin/WH<sub>2</sub> fold. β-thymosins may therefore have evolved by addition of novel C-terminal sequence to an ancestral WH<sub>2</sub> module.<ref name="pmid17468236">{{cite journal | vauthors = Dominguez R | title = The beta-thymosin/WH2 fold: multifunctionality and structure | journal = Annals of the New York Academy of Sciences | volume = 1112 | issue = 1| pages = 86–94 | date = September 2007 | pmid = 17468236 | doi = 10.1196/annals.1415.011 | bibcode = 2007NYASA1112...86D | s2cid = 222086831 }}</ref> However, sequence similarity searches designed to identify present-day WH2 domains<ref name="url_PF02205_tab2">{{cite web | url = http://pfam.sanger.ac.uk/family?acc=PF02205#tabview=tab2 | title = Family: WH2 (PF02205) | work = Pfam | publisher = Wellcome Trust Sanger Institute }}{{Dead link|date=June 2020 |bot=InternetArchiveBot |fix-attempted=yes }}</ref> fail to recognise β-thymosins, (and ''vice versa'') and the sequence and functional similarities may result from [[convergent evolution]].<ref name="pmid15328003">{{cite journal | vauthors = Edwards J | title = Are beta-thymosins WH2 domains? | journal = FEBS Lett. | volume = 573 | issue = 1–3 | pages = 231–2; author reply 233 | date = August 2004 | pmid = 15328003 | doi = 10.1016/j.febslet.2004.07.038 | doi-access = free }}</ref>


=== Biological activities of thymosin β<sub>4</sub>===
=== Biological activities of thymosin β<sub>4</sub>===


{{detail|Thymosin beta-4}}
The archetypical β-thymosin is β<sub>4</sub> (product in humans of the [[thymosin beta-4|TMSB4X]] gene), which is a major cellular constituent in many tissues. Its intracellular concentration may reach as high as 0.5&nbsp;mM.<ref name="pmid17468232">{{cite journal |author=Hannappel E |title=beta-Thymosins |journal=Ann. N. Y. Acad. Sci. |volume=1112 |issue= |pages=21–37 |year=2007 |month=September |pmid=17468232 |doi=10.1196/annals.1415.018 |url=http://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0077-8923&date=2007&volume=1112&spage=21}}</ref> Following [[Thymosin α1]], β<sub>4</sub> was the second of the biologically active peptides from Thymosin Fraction 5 to be completely sequenced and synthesized.<ref name="Low TL, Hu SK, Goldstein AL 1981 1162–6">{{cite journal |author=Low TL, Hu SK, Goldstein AL |title=Complete amino acid sequence of bovine thymosin beta 4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=78 |issue=2 |pages=1162–6 |year=1981 | month=February |pmid=6940133 |pmc=319967 |doi= 10.1073/pnas.78.2.1162|url=}}</ref>


Any concepts of the biological role of thymosin β<sub>4</sub> must inevitably be coloured by the demonstration that total ablation of the thymosin β<sub>4</sub> gene in the mouse allows apparently normal embryonic development of mice which are fertile as adults.<ref name="Banerjee-2012">{{Cite journal | last1 = Banerjee | first1 = I. | last2 = Zhang | first2 = J. | last3 = Moore-Morris | first3 = T. | last4 = Lange | first4 = S. | last5 = Shen | first5 = T. | last6 = Dalton | first6 = ND. | last7 = Gu | first7 = Y. | last8 = Peterson | first8 = KL. | last9 = Evans | first9 = SM. | title = Thymosin beta 4 is dispensable for murine cardiac development and function. | journal = Circ Res | volume = 110 | issue = 3 | pages = 456–64 | month = Feb | year = 2012 | doi = 10.1161/CIRCRESAHA.111.258616 | PMID = 22158707 }}</ref>
The archetypical β-thymosin is β<sub>4</sub> (product in humans of the [[thymosin beta-4|TMSB4X]] gene), which is a major cellular constituent in many tissues. Its intracellular concentration may reach as high as 0.5&nbsp;mM.<ref name="pmid17468232">{{cite journal | vauthors = Hannappel E | title = beta-Thymosins | journal = Annals of the New York Academy of Sciences | volume = 1112 | issue = 1| pages = 21–37 | date = September 2007 | pmid = 17468232 | doi = 10.1196/annals.1415.018 | bibcode = 2007NYASA1112...21H | s2cid = 222082792 }}</ref> Following [[Thymosin α1]], β<sub>4</sub> was the second of the biologically active peptides from Thymosin Fraction 5 to be completely sequenced and synthesized.<ref name="Low TL, Hu SK, Goldstein AL 1981 1162–6">{{cite journal | vauthors = Low TL, Hu SK, Goldstein AL | title = Complete amino acid sequence of bovine thymosin beta 4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 78 | issue = 2 | pages = 1162–6 | date = February 1981 | pmid = 6940133 | pmc = 319967 | doi = 10.1073/pnas.78.2.1162 | bibcode = 1981PNAS...78.1162L | doi-access = free }}</ref>


⚫
Due to its profusion in the [[cytosol]] and its ability to bind G-actin but not F-actin, thymosin β<sub>4</sub> is regarded as the principal actin-sequestering protein in many cell types.<ref name="Lodish_2000">{{cite book | author = Lodish, Harvey F. | title = Molecular cell biology | publisher = W.H. Freeman | location = San Francisco | year = 2000 | isbn = 978-0-7167-3706-3 | chapter = Chapter 18. Cell Motility and Shape I: Microfilaments. 18.2. The Dynamics of Actin Assembly | chapter-url = https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mcb.section.5145 | url-access = registration | url = https://archive.org/details/molecularcellbi000lodi }}</ref>
==== Actin binding ====

Thymosin β<sub>4</sub> was initially perceived as a thymic hormone. However this changed when it was discovered that it forms a 1:1 complex with G (globular) actin, and is present at high concentration in a wide range of mammalian cell types.<ref name="pmid1999398 ">{{cite journal |author=Safer D, Elzinga M, Nachmias VT |title=Thymosin beta 4 and Fx, an actin-sequestering peptide, are indistinguishable |journal=J. Biol. Chem. |volume=266 |issue=7 |pages=4029–32 |year=1991 |month=March |pmid=1999398 |doi= |url=}}</ref> When appropriate, G-actin monomers polymerize to form F (filamentous) actin which together with other proteins that bind to actin comprise cellular [[microfilaments]]. Formation by G-actin of the complex with β-thymosin (= "sequestration") opposes this.

⚫
Due to its profusion in the [[cytosol]] and its ability to bind G-actin but not F-actin, thymosin β<sub>4</sub> is regarded as the principal actin-sequestering protein in many cell types. Thymosin β<sub>4</sub> functions like a buffer for monomeric actin as represented in the following reaction:<ref name="Lodish_2000">{{cite book | author = Lodish, Harvey F. | authorlink = | editor = | others = | title = Molecular cell biology | edition = | language = | publisher = W.H. Freeman | location = San Francisco | year = 2000 | origyear = | pages = | quote = | isbn = 0-7167-3706-X | oclc = | doi = | url = | accessdate = | chapter = Chapter 18. Cell Motility and Shape I: Microfilaments. 18.2. The Dynamics of Actin Assembly | chapterurl = http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mcb.section.5145 }}</ref>

F-actin ↔ G-actin + Thymosin β<sub>4</sub> ↔ G-actin/Thymosin β<sub>4</sub>

Release of G-actin monomers from thymosin β<sub>4</sub> occurs as part of the mechanism that drives actin polymerization in the normal function of the [[cytoskeleton]] in cell [[morphology (biology)|morphology]] and [[cell motility]].

The sequence lkktet, which starts at residue 17 of the 43-aminoacid sequence of [[thymosin beta-4]], and is strongly conserved between all β-thymosins, together with a similar sequence in WH2 domains, is frequently referred to as "the actin-binding motif" of these proteins, although modelling based on X-ray crystallography has shown that essentially the entire length of the β-thymosin sequence interacts with actin in the actin-thymosin complex.<ref>{{cite journal |author=Xue B, Aguda AH, Robinson RC |title=Models of the actin-bound forms of the beta-thymosins |journal=Ann. N. Y. Acad. Sci. |volume=1112 |issue= |pages=56–66 |year=2007 |month=September |pmid=17468228 |doi=10.1196/annals.1415.010 |url=}}</ref>

===="Moonlighting"====

In addition to its intracellular role as the major actin-sequestering molecule in cells of many multicellular animals, thymosin β<sub>4</sub> shows a remarkably diverse range of effects when present in the fluid surrounding animal tissue cells. Taken together, these effects suggest that thymosin has a general role in tissue regeneration. This has suggested a variety of possible therapeutic applications, and several have now been extended to animal models and human clinical trials.

It is considered unlikely that thymosin β<sub>4</sub> exerts all these effects via intracellular sequestration of G-actin. This would require its uptake by cells, and moreover, in most cases the cells affected already have substantial intracellular concentrations.

The diverse activities related to tissue repair may depend on interactions with receptors quite distinct from actin and possessing extracellular ligand-binding domains. Such multi-tasking by, or "partner promiscuity" of, proteins has been referred to as [[protein moonlighting]].<ref>{{cite journal |author=Jeffery CJ |title=Moonlighting proteins |journal=Trends Biochem. Sci. |volume=24 |issue=1 |pages=8–11 |year=1999 |month=January |pmid=10087914 |doi= 10.1016/S0968-0004(98)01335-8|url=}}</ref> Proteins such as thymosins which lack stable folded structure in aqueous solution, are known as [[intrinsically unstructured proteins]] (IUPs). Because IUPs acquire specific folded structures only on binding to their partner proteins, they offer special possibilities for interaction with multiple partners.<ref>{{cite journal |author=Tompa P, Szász C, Buday L |title=Structural disorder throws new light on moonlighting |journal=Trends Biochem. Sci. |volume=30 |issue=9 |pages=484–9 |year=2005 |month=September |pmid=16054818 |doi=10.1016/j.tibs.2005.07.008 |url=}}</ref> A candidate extracellular receptor of high affinity for thymosin β<sub>4</sub> is the β subunit of cell surface-located [[ATP synthase]], which would allow extracellular thymosin to signal via a [[purinergic receptor]].<ref>{{cite journal |author=Freeman KW, Bowman BR, Zetter BR |title=Regenerative protein thymosin {beta}-4 is a novel regulator of purinergic signaling |journal=FASEB J |volume= 25|issue= 3|pages= 907–15|year=2010 |month=November |pmid=21106936 |doi=10.1096/fj.10-169417 |url=}}</ref>

Some of the multiple activities of thymosin β<sub>4</sub> unrelated to actin may be mediated by a tetrapeptide enzymically-cleaved from its N-terminus, N-acetyl-ser-asp-lys-pro, brand names Seraspenide or Goralatide, best known as an inhibitor of the proliferation of [[haematopoietic]] (blood-cell precursor) stem cells of bone marrow.

=====Tissue regeneration=====

Work with cell cultures and experiments with animals have shown that administration of thymosin β<sub>4</sub> can promote migration of cells, formation of blood vessels, maturation of stem cells, survival of various cell types and lowering of the production of [[pro-inflammatory cytokine]]s. These multiple properties have provided the impetus for a world-wide series of on-going [[clinical trial]]s of potential effectiveness of thymosin β<sub>4</sub> in promoting repair of wounds in skin, cornea and heart.<ref name="pmid20536453">{{cite journal | author = Philp D, Kleinman HK | title = Animal studies with thymosin beta, a multifunctional tissue repair and regeneration peptide | journal = Ann. N. Y. Acad. Sci. | volume = 1194 | issue = | pages = 81–6 | year = 2010 | month = April | pmid = 20536453 | doi = 10.1111/j.1749-6632.2010.05479.x }}</ref>

Such tissue-regenerating properties of thymosin β<sub>4</sub> may ultimately contribute to repair of human heart muscle damaged by heart disease and heart attack. In mice, administration of thymosin β<sub>4</sub> has been shown to stimulate formation of new heart muscle cells from otherwise inactive precursor cells present in the outer lining of adult hearts,<ref name=Smart_2011>{{cite journal | author = Smart N, Bollini D, Dubé KN, Vieira JM, Zhou B, Davidson S, Yellon D, Riegler J, Price AN, Lythgoe MF, Pu WT, Riley PR | title = De novo cardiomyocytes from within the activated adult heart after injury | journal = Nature | year = 2011 | month = June | pmid = 21654746 | volume = 474 | doi = 10.1038/nature10188 | laysummary = http://www.bbc.co.uk/news/health-13699711 | laysource = BBC News | issue=7353}}</ref> to induce migration of these cells into heart muscle<ref name="pmid19235142">{{cite journal | author = Smart N, Riley PR | title = Derivation of epicardium-derived progenitor cells (EPDCs) from adult epicardium | journal = Curr Protoc Stem Cell Biol | volume = Chapter 2 | issue = | pages = Unit2C.2 | year = 2009 | month = February | pmid = 19235142 | doi = 10.1002/9780470151808.sc02c02s8 }}</ref> and recruit new blood vessels within the muscle.<ref name="pmid19909250">{{cite journal | author = Riley PR, Smart N | title = Thymosin beta4 induces epicardium-derived neovascularization in the adult heart | journal = Biochem. Soc. Trans. | volume = 37 | issue = Pt 6 | pages = 1218–20 | year = 2009 | month = December | pmid = 19909250 | doi = 10.1042/BST0371218 }}</ref>

=====Anti-inflammatory role for sulfoxide=====

In 1999 researchers in Glasgow University found that an oxidised derivative of thymosin β<sub>4</sub> (the [[sulfoxide]], in which an oxygen atom is added to the [[methionine]] near the N-terminus) exerted several potentially [[anti-inflammatory]] effects on [[neutrophil]] leucocytes. It promoted their dispersion from a focus, inhibited their response to a small peptide ([[F-Met]]-Leu-Phe) which attracts them to sites of bacterial infection and lowered their adhesion to [[endothelial]] cells. (Adhesion to endothelial cells of blood vessel walls is pre-requisite for these cells to leave the bloodstream and invade infected tissue). A possible anti-inflammatory role for the β<sub>4</sub> sulfoxide was supported by the group's finding that it counteracted artificially-induced inflammation in mice.

The group had first identified the thymosin sulfoxide as an active factor in culture fluid of cells responding to treatment with a [[steroid hormone]], suggesting that its formation might form part of the mechanism by which steroids exert anti-inflammatory effects. Extracellular thymosin β<sub>4</sub> would be readily oxidised to the sulfoxide ''in vivo'' at sites of inflammation, by the [[respiratory burst]].<ref>{{cite journal |author=Young JD, Lawrence AJ, MacLean AG, ''et al.'' |title=Thymosin beta 4 sulfoxide is an anti-inflammatory agent generated by monocytes in the presence of glucocorticoids |journal=Nat. Med. |volume=5 |issue=12 |pages=1424–7 |year=1999 |month=December |pmid=10581087 |doi=10.1038/71002 |url=}}.</ref>

=====Terminal deoxynucleotidyl transferase=====

Thymosin β<sub>4</sub> induces the activity of the enzyme [[terminal deoxynucleotidyl transferase]] in populations of [[thymocytes]] (thymus-derived [[lymphocytes]]). This suggests that the peptide may contribute to the maturation of these cells.<ref name="Low TL, Hu SK, Goldstein AL 1981 1162–6"/>


=== Clinical applications ===
=== Clinical applications ===


Thymosin β<sub>4</sub> has been tested in multicenter trials sponsored jointly by RegeneRx Biopharmaceuticals Inc (Rockville, MD, USA) and Sigma Tau (Pomezia, Italy) in the United States and Europe in patients with [[bed sores]], ulcers caused by [[venostasis]], and [[Epidermolysis bullosa simplex]] and was found to accelerate bed sore and stasis ulcer repair by one month. The epidermolysis bullosa trial is still enrolling. It has also been tested in patients with chronic neurotrophic corneal epithelial defects and found to promote repair.
Thymosin β<sub>4</sub> has been tested in multicenter trials sponsored jointly by RegeneRx Biopharmaceuticals Inc (Rockville, MD, USA) and Sigma Tau (Pomezia, Italy) in the United States and Europe in patients with [[bed sores]], ulcers caused by [[venostasis]], and [[Epidermolysis bullosa simplex]] and was found to accelerate bed sore and stasis ulcer repair by one month.
It has also been tested in patients with chronic neurotrophic corneal epithelial defects and found to promote repair.

⚫
Levels of human thymosin β<sub>15</sub> in urine have shown promise as a diagnostic marker for prostate cancer which is sensitive to potential aggressiveness of the tumour <ref>{{cite journal |author=Hutchinson LM, Chang EL, Becker CM, ''et al.'' |title=Use of thymosin beta15 as a urinary biomarker in human prostate cancer |journal=Prostate |volume=64 |issue=2 |pages=116–27 |year=2005 |month=July |pmid=15666387 |doi=10.1002/pros.20202 |url=}}</ref>

⚫
===Doping in Sports===
⚫
Thymosin beta-4 was allegedly used by some players in various Australian football codes and is under investigation by the Australian Sports Anti-Doping Authority for anti-doping violations <ref>{{cite web |url=https://theconversation.edu.au/cronulla-sharks-and-thymosin-beta-4-is-it-doping-12694 |title= Cronulla Sharks and thymosin beta-4 … is it doping? |format= |work= |accessdate= }} </ref>


⚫
'''Thymosin β<sub>15</sub>''' : Levels of human thymosin β<sub>15</sub> in urine have shown promise as a diagnostic marker for [[prostate cancer]] which is sensitive to potential aggressiveness of the tumour <ref>{{cite journal | vauthors = Hutchinson LM, Chang EL, Becker CM, Shih MC, Brice M, DeWolf WC, Gaston SM, Zetter BR | title = Use of thymosin beta15 as a urinary biomarker in human prostate cancer | journal = Prostate | volume = 64 | issue = 2 | pages = 116–27 | date = July 2005 | pmid = 15666387 | doi = 10.1002/pros.20202 | s2cid = 71453198 }}</ref>


⚫
===Doping in sports===
⚫
[[Thymosin beta-4]] was allegedly used by some players in various Australian football codes.<ref>{{cite web |url=https://theconversation.edu.au/cronulla-sharks-and-thymosin-beta-4-is-it-doping-12694 |title= Cronulla Sharks and thymosin beta-4 … is it doping? }}</ref>


== β-thymosin repeat proteins ==
== β-thymosin repeat proteins ==
⚫
[[File:Thymosin 1HJ0.png|thumb|[[Protein nuclear magnetic resonance spectroscopy|NMR structure]] of bovine β9-thymosin, the orthologue of human β10.<ref name="1HJ0.pdb2">{{PDB|1HJ0}}; {{cite journal|date=May 1997|title=Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy|journal=Biopolymers|volume=41|issue=6|pages=623–34|doi=10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S|pmid=9108730|quote=The thymosin is β9, bovine orthologue of human β10. Stabilised by organic solvent, the structure was determined by NMR. (Free β-thymosins lack a stable fold in solution)|vauthors=Stoll R, Voelter W, Holak TA|doi-access=free}}</ref> Rainbow coloured where the [[N-terminus]] = blue and the [[C-terminus]] = red.]]


=== Distribution ===
=== Distribution ===
These proteins, which typically contain 2-4 repeats of the β-thymosin sequence, are found in all phyla of the animal kingdom, with the probable exception of sponges<ref name="isbn0-387-46407-7">{{cite book | author = Pekka Lappalainen | title = Actin-Monomer-Binding Proteins | publisher = Landes Bioscience and Springer Science+Business Media, LLC | location = Boston, MA | year = 2007 | pages = | isbn = 0-387-46407-7 }}</ref> The sole mammalian example, a dimer in mice, is synthesised by transcriptional read-through between two copies of the mouse β15 gene, each of which is also transcribed separately.<ref name="pmid19233202">{{cite journal | author = Dhaese S, Vandepoele K, Waterschoot D, Vanloo B, Vandekerckhove J, Ampe C, Van Troys M | title = The mouse thymosin beta15 gene family displays unique complexity and encodes a functional thymosin repeat | journal = J. Mol. Biol. | volume = 387 | issue = 4 | pages = 809–25 | year = 2009 | month = April | pmid = 19233202 | doi = 10.1016/j.jmb.2009.02.026 }}</ref> A uniquely multiple example is the protein thypedin of ''Hydra'' which has 27 repeats of a β-thymosin sequence.<ref name="pmid16169708">{{cite journal | author = Herrmann D, Hatta M, Hoffmeister-Ullerich SA | title = Thypedin, the multi copy precursor for the hydra peptide pedin, is a beta-thymosin repeat-like domain containing protein | journal = Mech. Dev. | volume = 122 | issue = 11 | pages = 1183–93 | year = 2005 | month = November | pmid = 16169708 | doi = 10.1016/j.mod.2005.07.003 }}</ref>
These proteins, which typically contain 2-4 repeats of the β-thymosin sequence, are found in all phyla of the animal kingdom, with the probable exception of sponges<ref name="isbn0-387-46407-7">{{cite book | author = Pekka Lappalainen | title = Actin-Monomer-Binding Proteins | publisher = Landes Bioscience and Springer Science+Business Media, LLC | location = Boston, MA | year = 2007 | isbn = 978-0-387-46407-7 }}</ref> The sole mammalian example, a dimer in mice, is synthesised by transcriptional read-through between two copies of the mouse β15 gene, each of which is also transcribed separately.<ref name="pmid19233202">{{cite journal | vauthors = Dhaese S, Vandepoele K, Waterschoot D, Vanloo B, Vandekerckhove J, Ampe C, Van Troys M | title = The mouse thymosin beta15 gene family displays unique complexity and encodes a functional thymosin repeat | journal = J. Mol. Biol. | volume = 387 | issue = 4 | pages = 809–25 | date = April 2009 | pmid = 19233202 | doi = 10.1016/j.jmb.2009.02.026 }}</ref> A uniquely multiple example is the protein thypedin of ''Hydra'' which has 27 repeats of a β-thymosin sequence.<ref name="pmid16169708">{{cite journal | vauthors = Herrmann D, Hatta M, Hoffmeister-Ullerich SA | title = Thypedin, the multi copy precursor for the hydra peptide pedin, is a beta-thymosin repeat-like domain containing protein | journal = Mech. Dev. | volume = 122 | issue = 11 | pages = 1183–93 | date = November 2005 | pmid = 16169708 | doi = 10.1016/j.mod.2005.07.003 | doi-access = free }}</ref>


=== Biological activities ===
=== Biological activities ===
β-thymosin repeat proteins resemble the monomeric forms in being able to bind to actin, but sequence differences in one example studied, a three-repeat protein [[Ciboulot]] of the fruit fly'' [[Drosophila]],'' allow binding to ends of actin filaments, an activity which differs from monomer sequestration.<ref name="pmid17947587">{{cite journal | author = Carlier MF, Hertzog M, Didry D, Renault L, Cantrelle FX, van Heijenoort C, Knossow M, Guittet E | title = Structure, function, and evolution of the beta-thymosin/WH2 (WASP-Homology2) actin-binding module | journal = Ann. N. Y. Acad. Sci. | volume = 1112 | issue = | pages = 67–75 | year = 2007 | month = September | pmid = 17947587 | doi = 10.1196/annals.1415.037 }}</ref>
β-thymosin repeat proteins resemble the monomeric forms in being able to bind to actin, but sequence differences in one example studied, a three-repeat protein [[Ciboulot]] of the fruit fly'' [[Drosophila]],'' allow binding to ends of actin filaments, an activity which differs from monomer sequestration.<ref name="pmid17947587">{{cite journal | vauthors = Carlier MF, Hertzog M, Didry D, Renault L, Cantrelle FX, van Heijenoort C, Knossow M, Guittet E | title = Structure, function, and evolution of the beta-thymosin/WH2 (WASP-Homology2) actin-binding module | journal = Annals of the New York Academy of Sciences | volume = 1112 | issue = 1| pages = 67–75 | date = September 2007 | pmid = 17947587 | doi = 10.1196/annals.1415.037 | bibcode = 2007NYASA1112...67C | s2cid = 22521722 }}</ref>


These proteins became of interest in neurobiology with the finding that in the nudibranch (sea slug) ''[[Hermissenda crassicornis]]'', the protein Csp24 (conditioned stimulus pathway phosphoprotein-24), with 4 repeats, is involved in simple forms of learning: both one-trial enhancement of the excitability of [[sensory neurons]] in the conditioned stimulus pathway,<ref name="pmid17681698">{{cite journal | author = Redell JB, Xue-Bian JJ, Bubb MR, Crow T | title = One-trial in vitro conditioning regulates an association between the beta-thymosin repeat protein Csp24 and actin | journal = Neuroscience | volume = 148 | issue = 2 | pages = 413–20 | year = 2007 | month = August | pmid = 17681698 | doi = 10.1016/j.neuroscience.2007.06.023 }}</ref> and in multi-trial Pavlovian conditioning.<ref name="www.ncbi.nlm.nih.gov">{{Cite web | last = | first = | title = Proteomic Analysis of Post-Translational Modifications in Conditioned Hermissenda | url = http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815081/ | publisher = | date = | accessdate = 6 November 2011 }}</ref> The phosphorylation of Csp24, in common with post-translational modifications of a number of cytoskeleton-related proteins may contribute to actin-filament dynamics underlying structural remodeling of responsive cells.<ref name="pmid19961907">{{cite journal |author=Crow T, Xue-Bian JJ |title=Proteomic analysis of post-translational modifications in conditioned Hermissenda |journal=Neuroscience |volume=165 |issue=4 |pages=1182–90 |year=2010 |month=February |pmid=19961907 |pmc=2815081 |doi=10.1016/j.neuroscience.2009.11.066 |url=}}</ref>
These proteins became of interest in neurobiology with the finding that in the nudibranch (sea slug) ''[[Hermissenda crassicornis]]'', the protein Csp24 (conditioned stimulus pathway phosphoprotein-24), with 4 repeats, is involved in simple forms of learning: both one-trial enhancement of the excitability of [[sensory neurons]] in the conditioned stimulus pathway,<ref name="pmid17681698">{{cite journal | vauthors = Redell JB, Xue-Bian JJ, Bubb MR, Crow T | title = One-trial in vitro conditioning regulates an association between the beta-thymosin repeat protein Csp24 and actin | journal = Neuroscience | volume = 148 | issue = 2 | pages = 413–20 | date = August 2007 | pmid = 17681698 | doi = 10.1016/j.neuroscience.2007.06.023 | s2cid = 35463597 }}</ref> and in multi-trial Pavlovian conditioning.<ref name="Crow_2010">{{cite journal | vauthors = Crow T, Xue-Bian JJ | title = Proteomic Analysis of Post-Translational Modifications in Conditioned Hermissenda | journal = Neuroscience | volume = 165 | issue = 4 | pages = 1182–90 | date = February 2010 | pmid = 19961907 | pmc = 2815081 | doi = 10.1016/j.neuroscience.2009.11.066 }}</ref> The phosphorylation of Csp24, in common with post-translational modifications of a number of cytoskeleton-related proteins may contribute to actin-filament dynamics underlying structural remodeling of responsive cells.<ref name="Crow_2010"/>


== Picture gallery ==
== See also ==
⚫
* [[Thymosins]]
<gallery>
⚫
** [[Thymosin α1]]
⚫
File:Thymosin 1HJ0.png|[[Protein nuclear magnetic resonance spectroscopy|NMR structure]] of bovine β9-thymosin, the orthologue of human β10.<ref name="1HJ0.pdb">{{PDB|1HJ0}}; {{cite journal | author = Stoll R, Voelter W, Holak TA | title = Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy | journal = Biopolymers | volume = 41 | issue = 6 | pages = 623–34 | year = 1997 | month = May | pmid = 9108730 | doi = 10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S | quote = The thymosin is β9, bovine orthologue of human β10. Stabilised by organic solvent, the structure was determined by NMR. (Free β-thymosins lack a stable fold in solution) | issn = }}</ref> Rainbow coloured where the [[N-terminus]] = blue and the [[C-terminus]] = red.
** [[Thymosin beta-4]]
</gallery>


==References==
== References ==
{{reflist|2}}
{{reflist|2}}


Latest revision as of 17:08, 8 November 2023

alt text
NMR structure of a β-thymosin. Both thymosin α1 [1] and β-thymosins are intrinsically unstructured proteins, i.e. they lack a stable fold when free in aqueous solution. This structure, mostly alpha helix, was artificially stabilised by an organic solvent.[2] The thymosin illustrated, originally named β9 is the cow orthologue of human β10

Beta thymosins are a family of proteins which have in common a sequence of about 40 amino acids similar to the small protein thymosin β4. They are found almost exclusively in multicellular animals. Thymosin β4 was originally obtained from the thymus in company with several other small proteins which although named collectively "thymosins" are now known to be structurally and genetically unrelated and present in many different animal tissues.

Single domain β-thymosins

[edit]

Distribution

[edit]
Thymosin beta-4 family
Structure of thymosin beta 9.[3]
Identifiers
SymbolThymosin
PfamPF01290
InterProIPR001152
SMARTSM00152
PROSITEPDOC00433
SCOP21hj0 / SCOPe / SUPFAM
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary
PDB1hj0​, 1sqk​, 1t44​, 2ff6​

Monomeric β-thymosins, i.e. those of molecular weight similar to the peptides originally isolated from thymus by Goldstein, are found almost exclusively in cells of multicellular animals.[4] Known exceptions are monomeric thymosins found in a few single-celled organisms, significantly those currently regarded as the closest relatives of multicellular animals:[5] choanoflagellates [6] and filastereans.[7] Although found in very early-diverged animals such as sponges, monomeric thymosins are absent from arthropods and nematodes, which do nevertheless possess "β-thymosin repeat proteins" which are constructed from several end-to-end repeats of β-thymosin sequences.[8] Genomics has shown that tetrapods (land vertebrates) each express three monomeric β-thymosins, which are the animal species' equivalents (orthologues) of human β4, β10 and β15 thymosins, respectively. The human thymosins are encoded by the genes TMSB4X, TMSB10 and TMSB15A and TMSB15B. (In humans, the proteins encoded by the two TMSB15 genes are identical.) Bony fish in general express orthologues of these same three, plus an additional copy of the β4 orthologue.[9]

family gene locus protein
β4 TMSB4X Chr. X q21.3-q22 Thymosin β4
TMSB4Y Chr. Y Thymosin β4,
Y-chromosomal
β10 TMSB10 Chr. 2 p11.2 Thymosin β10
β15 TMSB15A Chr. X q21.33-q22.3 Thymosin β15
TMSB15B Chr. X q22.2 Thymosin β15

Thymosin β1 was found to be ubiquitin (truncated by two C-terminal glycine residues).[10]

Relation to the WH2 sequence module

[edit]

The N-terminal half of β-thymosins bears a strong similarity in amino acid sequence to a very widely distributed sequence module, the WH2 module. (Wasp Homology Domain 2 - the name is derived from Wiskott-Aldrich syndrome protein).[11][12] Evidence from X-ray crystallography shows that this part of β-thymosins binds to actin in a near-identical manner to that of WH2 modules, both adopting as they bind, a conformation which has been referred to as the β-thymosin/WH2 fold. β-thymosins may therefore have evolved by addition of novel C-terminal sequence to an ancestral WH2 module.[13] However, sequence similarity searches designed to identify present-day WH2 domains[14] fail to recognise β-thymosins, (and vice versa) and the sequence and functional similarities may result from convergent evolution.[15]

Biological activities of thymosin β4

[edit]
Further information: Thymosin beta-4

The archetypical β-thymosin is β4 (product in humans of the TMSB4X gene), which is a major cellular constituent in many tissues. Its intracellular concentration may reach as high as 0.5 mM.[10] Following Thymosin α1, β4 was the second of the biologically active peptides from Thymosin Fraction 5 to be completely sequenced and synthesized.[16]

Due to its profusion in the cytosol and its ability to bind G-actin but not F-actin, thymosin β4 is regarded as the principal actin-sequestering protein in many cell types.[17]

Clinical applications

[edit]

Thymosin β4 has been tested in multicenter trials sponsored jointly by RegeneRx Biopharmaceuticals Inc (Rockville, MD, USA) and Sigma Tau (Pomezia, Italy) in the United States and Europe in patients with bed sores, ulcers caused by venostasis, and Epidermolysis bullosa simplex and was found to accelerate bed sore and stasis ulcer repair by one month. It has also been tested in patients with chronic neurotrophic corneal epithelial defects and found to promote repair.

Thymosin β15 : Levels of human thymosin β15 in urine have shown promise as a diagnostic marker for prostate cancer which is sensitive to potential aggressiveness of the tumour [18]

Doping in sports

[edit]

Thymosin beta-4 was allegedly used by some players in various Australian football codes.[19]

β-thymosin repeat proteins

[edit]
NMR structure of bovine β9-thymosin, the orthologue of human β10.[20] Rainbow coloured where the N-terminus = blue and the C-terminus = red.

Distribution

[edit]

These proteins, which typically contain 2-4 repeats of the β-thymosin sequence, are found in all phyla of the animal kingdom, with the probable exception of sponges[21] The sole mammalian example, a dimer in mice, is synthesised by transcriptional read-through between two copies of the mouse β15 gene, each of which is also transcribed separately.[22] A uniquely multiple example is the protein thypedin of Hydra which has 27 repeats of a β-thymosin sequence.[23]

Biological activities

[edit]

β-thymosin repeat proteins resemble the monomeric forms in being able to bind to actin, but sequence differences in one example studied, a three-repeat protein Ciboulot of the fruit fly Drosophila, allow binding to ends of actin filaments, an activity which differs from monomer sequestration.[24]

These proteins became of interest in neurobiology with the finding that in the nudibranch (sea slug) Hermissenda crassicornis, the protein Csp24 (conditioned stimulus pathway phosphoprotein-24), with 4 repeats, is involved in simple forms of learning: both one-trial enhancement of the excitability of sensory neurons in the conditioned stimulus pathway,[25] and in multi-trial Pavlovian conditioning.[26] The phosphorylation of Csp24, in common with post-translational modifications of a number of cytoskeleton-related proteins may contribute to actin-filament dynamics underlying structural remodeling of responsive cells.[26]

See also

[edit]

References

[edit]
  1. ^ Grottesi A, Sette M, Palamara T, Rotilio G, Garaci E, Paci M (1998). "The conformation of peptide thymosin alpha 1 in solution and in a membrane-like environment by circular dichroism and NMR spectroscopy. A possible model for its interaction with the lymphocyte membrane". Peptides. 19 (10): 1731–8. doi:10.1016/S0196-9781(98)00132-6. PMID 9880079. S2CID 39655090.
  2. ^ PDB: 1HJ0​; Stoll R, Voelter W, Holak TA (May 1997). "Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy". Biopolymers. 41 (6): 623–34. doi:10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S. PMID 9108730. The thymosin is β9, bovine orthologue of human β10. Stabilised by organic solvent, the structure was determined by NMR. (Free β-thymosins lack a stable fold in solution)
  3. ^ Stoll R, Voelter W, Holak TA (May 1997). "Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy". Biopolymers. 41 (6): 623–34. doi:10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S. PMID 9108730.
  4. ^ "Family: Thymosin (PF01290)". Pfam. Wellcome Trust Sanger Institute. Archived from the original on 2008-01-26.
  5. ^ Shalchian-Tabrizi K, Minge MA, Espelund M, Orr R, Ruden T, Jakobsen KS, Cavalier-Smith T (2008). "Multigene phylogeny of choanozoa and the origin of animals". PLOS ONE. 3 (5): e2098. Bibcode:2008PLoSO...3.2098S. doi:10.1371/journal.pone.0002098. PMC 2346548. PMID 18461162.
  6. ^ "XYM2758.rev XYM Monosiga brevicollis rapidly growi... - EST result". 2008-03-20. {{cite journal}}: Cite journal requires |journal= (help)
  7. ^ "NUE00005552 Capsaspora owczarzaki Amplicon express Capsaspora owczarza - EST - NCBI". 2008-11-20. {{cite journal}}: Cite journal requires |journal= (help).
  8. ^ Manuel M, Kruse M, Müller WE, Le Parco Y (October 2000). "The comparison of beta-thymosin homologues among metazoa supports an arthropod-nematode clade". J. Mol. Evol. 51 (4): 378–81. Bibcode:2000JMolE..51..378M. doi:10.1007/s002390010100. PMID 11040289. S2CID 8675540.
  9. ^ Edwards J (March 2010). "Vertebrate beta-thymosins: conserved synteny reveals the relationship between those of bony fish and of land vertebrates". FEBS Lett. 584 (5): 1047–53. doi:10.1016/j.febslet.2010.02.004. PMID 20138884.
  10. ^ a b Hannappel E (September 2007). "beta-Thymosins". Annals of the New York Academy of Sciences. 1112 (1): 21–37. Bibcode:2007NYASA1112...21H. doi:10.1196/annals.1415.018. PMID 17468232. S2CID 222082792.
  11. ^ Paunola E, Mattila PK, Lappalainen P (February 2002). "WH2 domain: a small, versatile adapter for actin monomers". FEBS Lett. 513 (1): 92–7. doi:10.1016/S0014-5793(01)03242-2. PMID 11911886.
  12. ^ "Family: WH2 (PF02205)". Pfam. Wellcome Trust Sanger Institute.[permanent dead link‍]
  13. ^ Dominguez R (September 2007). "The beta-thymosin/WH2 fold: multifunctionality and structure". Annals of the New York Academy of Sciences. 1112 (1): 86–94. Bibcode:2007NYASA1112...86D. doi:10.1196/annals.1415.011. PMID 17468236. S2CID 222086831.
  14. ^ "Family: WH2 (PF02205)". Pfam. Wellcome Trust Sanger Institute.[permanent dead link‍]
  15. ^ Edwards J (August 2004). "Are beta-thymosins WH2 domains?". FEBS Lett. 573 (1–3): 231–2, author reply 233. doi:10.1016/j.febslet.2004.07.038. PMID 15328003.
  16. ^ Low TL, Hu SK, Goldstein AL (February 1981). "Complete amino acid sequence of bovine thymosin beta 4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations". Proceedings of the National Academy of Sciences of the United States of America. 78 (2): 1162–6. Bibcode:1981PNAS...78.1162L. doi:10.1073/pnas.78.2.1162. PMC 319967. PMID 6940133.
  17. ^ Lodish, Harvey F. (2000). "Chapter 18. Cell Motility and Shape I: Microfilaments. 18.2. The Dynamics of Actin Assembly". Molecular cell biology. San Francisco: W.H. Freeman. ISBN 978-0-7167-3706-3.
  18. ^ Hutchinson LM, Chang EL, Becker CM, Shih MC, Brice M, DeWolf WC, Gaston SM, Zetter BR (July 2005). "Use of thymosin beta15 as a urinary biomarker in human prostate cancer". Prostate. 64 (2): 116–27. doi:10.1002/pros.20202. PMID 15666387. S2CID 71453198.
  19. ^ "Cronulla Sharks and thymosin beta-4 … is it doping?".
  20. ^ PDB: 1HJ0​; Stoll R, Voelter W, Holak TA (May 1997). "Conformation of thymosin beta 9 in water/fluoroalcohol solution determined by NMR spectroscopy". Biopolymers. 41 (6): 623–34. doi:10.1002/(SICI)1097-0282(199705)41:6<623::AID-BIP3>3.0.CO;2-S. PMID 9108730. The thymosin is β9, bovine orthologue of human β10. Stabilised by organic solvent, the structure was determined by NMR. (Free β-thymosins lack a stable fold in solution)
  21. ^ Pekka Lappalainen (2007). Actin-Monomer-Binding Proteins. Boston, MA: Landes Bioscience and Springer Science+Business Media, LLC. ISBN 978-0-387-46407-7.
  22. ^ Dhaese S, Vandepoele K, Waterschoot D, Vanloo B, Vandekerckhove J, Ampe C, Van Troys M (April 2009). "The mouse thymosin beta15 gene family displays unique complexity and encodes a functional thymosin repeat". J. Mol. Biol. 387 (4): 809–25. doi:10.1016/j.jmb.2009.02.026. PMID 19233202.
  23. ^ Herrmann D, Hatta M, Hoffmeister-Ullerich SA (November 2005). "Thypedin, the multi copy precursor for the hydra peptide pedin, is a beta-thymosin repeat-like domain containing protein". Mech. Dev. 122 (11): 1183–93. doi:10.1016/j.mod.2005.07.003. PMID 16169708.
  24. ^ Carlier MF, Hertzog M, Didry D, Renault L, Cantrelle FX, van Heijenoort C, Knossow M, Guittet E (September 2007). "Structure, function, and evolution of the beta-thymosin/WH2 (WASP-Homology2) actin-binding module". Annals of the New York Academy of Sciences. 1112 (1): 67–75. Bibcode:2007NYASA1112...67C. doi:10.1196/annals.1415.037. PMID 17947587. S2CID 22521722.
  25. ^ Redell JB, Xue-Bian JJ, Bubb MR, Crow T (August 2007). "One-trial in vitro conditioning regulates an association between the beta-thymosin repeat protein Csp24 and actin". Neuroscience. 148 (2): 413–20. doi:10.1016/j.neuroscience.2007.06.023. PMID 17681698. S2CID 35463597.
  26. ^ a b Crow T, Xue-Bian JJ (February 2010). "Proteomic Analysis of Post-Translational Modifications in Conditioned Hermissenda". Neuroscience. 165 (4): 1182–90. doi:10.1016/j.neuroscience.2009.11.066. PMC 2815081. PMID 19961907.
Retrieved from "https://en.wikipedia.org/enwiki/w/index.php?title=Beta_thymosins&oldid=1184150244"