Tre recombinase: Difference between revisions
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'''Tre recombinase''' is an experimental [[enzyme]] that in lab tests has removed [[DNA]] inserted by [[HIV]] from infected cells.<ref name="pmid17600219">{{cite journal |first1=Indrani |last1=Sarkar |first2=Ilona |last2=Hauber |first3=Joachim |last3=Hauber |first4=Frank |last4=Buchholz |year=2007 |title=HIV-1 proviral DNA excision using an evolved recombinase |journal=[[Science (journal)|Science]] |volume=316 |issue=5833 |pages=1912–15 |pmid=17600219 |doi=10.1126/science.1141453 |bibcode=2007Sci...316.1912S |s2cid=2437602 }}</ref> Through [[directed evolution|selective mutation]], [[Cre recombinase]] which recognizes loxP sites are modified to identify HIV [[long terminal repeats]] (loxLTR) instead. As a result, instead of performing [[Cre-Lox recombination]], the new enzyme performs recombination at HIV [[provirus]] sites.<ref>{{cite journal |title=Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice |first1=Ilona |last1=Hauber |first2=Helga |last2=Hofmann-Sieber |first3=Jan |last3=Chemnitz |display-authors=3 |first4=Danilo |last4=Dubrau |journal=PLOS Pathogens |volume = 9|issue = 9|pages = e1003587|date=September 26, 2013 |doi=10.1371/journal.ppat.1003587 |pmid = 24086129|pmc=3784474 |doi-access=free }}</ref> |
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'''Tre recombinase''' is an experimental [[enzyme]] that in lab tests has successfully removed [[DNA]] inserted by [[HIV]] from infected cells. The enzyme was derived from [[Cre recombinase]]. |
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The structure of Tre in complex with loxLTR has been resolved ({{PDB|5U91}}), allowing for analyzing the roles of individual mutations.<ref>{{cite journal |last1=Meinke |first1=G |last2=Karpinski |first2=J |last3=Buchholz |first3=F |last4=Bohm |first4=A |title=Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA. |journal=Nucleic Acids Research |date=19 September 2017 |volume=45 |issue=16 |pages=9726–9740 |doi=10.1093/nar/gkx603 |pmid=28934476 |pmc=5766204 |doi-access=free}}</ref> |
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==References== |
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{{Reflist}} |
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==External links== |
==External links== |
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* {{cite news |url=http://www.sciam.com/article.cfm?articleID=737AB56E-E7F2-99DF-382B756D1860EACA&chanID=sa003 |title=Designer enzyme cuts HIV out of infected cells |work=[[Scientific American]] |date=June 28, 2007 |first=JR |last=Minkel }} |
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* [http://www.physorg.com/news102261327.html Potential cure for HIV discovered] |
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* [http://www.sciencemag.org/cgi/content/full/sci;316/5833/1912 HIV-1 Proviral DNA Excision Using an Evolved Recombinase] |
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{{Antiretroviral drug}} |
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[[Category:Genetics]] |
[[Category:Genetics]] |
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[[Category:Molecular biology]] |
[[Category:Molecular biology]] |
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Latest revision as of 01:33, 29 November 2023
Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells.[1] Through selective mutation, Cre recombinase which recognizes loxP sites are modified to identify HIV long terminal repeats (loxLTR) instead. As a result, instead of performing Cre-Lox recombination, the new enzyme performs recombination at HIV provirus sites.[2]
The structure of Tre in complex with loxLTR has been resolved (PDB: 5U91), allowing for analyzing the roles of individual mutations.[3]
References
[edit]- ^ Sarkar, Indrani; Hauber, Ilona; Hauber, Joachim; Buchholz, Frank (2007). "HIV-1 proviral DNA excision using an evolved recombinase". Science. 316 (5833): 1912–15. Bibcode:2007Sci...316.1912S. doi:10.1126/science.1141453. PMID 17600219. S2CID 2437602.
- ^ Hauber, Ilona; Hofmann-Sieber, Helga; Chemnitz, Jan; et al. (September 26, 2013). "Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice". PLOS Pathogens. 9 (9): e1003587. doi:10.1371/journal.ppat.1003587. PMC 3784474. PMID 24086129.
- ^ Meinke, G; Karpinski, J; Buchholz, F; Bohm, A (19 September 2017). "Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA". Nucleic Acids Research. 45 (16): 9726–9740. doi:10.1093/nar/gkx603. PMC 5766204. PMID 28934476.
External links
[edit]- Minkel, JR (June 28, 2007). "Designer enzyme cuts HIV out of infected cells". Scientific American.
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