Talk:Nuclease

Cleanup

OK, I'm new to doing anything in Wikipedia bar reading it, so please bear with the newbie!

I've got several points on this page, though lack the skill to do it, and would appreciate others views before I risk learning in depth how to do it!

1) This page talks almost exclusively about restriction endonucleases, and the 'restriction nuclease' link comes through to this page. I would suggest the page is renamed 'Restrction Endonuclease' and a seperate page created to cover the broader 'nucleases' family.

2) The citations for Linn and Arber's two papers on the methylation-restriction system in the late 1960s are:

Linn S., Arber, W. (1968). Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form. Proc. Natl. Acad. Sci. USA. 59:1300-1306

Arber, W., Linn S. (1969) DNA modification and restriction. Annu. Rev. Biochem. 38:467-500

3) In the introduction, there is the sentence "One of these enzymes cleaved methylated DNA, while the other cleaved unmethylated DNA at a wide variety of locations along the length of the molecule". I believe this is incorrect- the first enzyme (methylase) adds a methyl group to the DNA, and is linked to an accurate page for 'methylase'.

4) In the 'Site-specific nuclease' section is the phrase 'The term "endonuclease" applies to sequence specific nucleases that break nucleic acid chains somewhere in the interior...'. Internal cutting of the nucleic acid is correct, but endonucleases are not necessarily sequence specific.

Thank you for listening. England Expects 20:10, 28 February 2007 (UTC)

Point 3 was incorrectly changed from 'methylated DNA' to 'cleaved methylated DNA' during Revision as of 15:00, 24 May 2006. I still don't want to mess up the page, though, so I'll wait for someone more responsible to come along. England Expects 20:22, 28 February 2007 (UTC)

I'm not sure whether we should really be saying nucleases gave birth to the biotechnology industry - I think we'd be better putting in a reference to molecular biology, which is the field I think it's really risen rise to. Thoughts? England Expects (talk) 17:00, 4 April 2008 (UTC)

Maybe nucleases gave birth to the Genetic engineering? (Redeemer079 17:54, 4 April 2008 (UTC))

Yes, I think that would be perfect. England Expects (talk) 10:03, 9 April 2008 (UTC)

There needs to be resolution of Exonuclease, Endonuclease, Nuclease, and Restriction Endonuclease. As of now, the categorizations are muddy and inconsistent. —Preceding unsigned comment added by 99.157.202.150 (talk) 17:40, 7 April 2008 (UTC)

Cleanup References

Noticed a problem, but didn't have time to fix it. References 5 - 12 seems to be the same reference. Jajava (talk) 22:29, 31 August 2018 (UTC)

Picture

A picture would be helpful here. Jobonki (talk) 21:37, 4 December 2014 (UTC)