Talk:DNA sequencing: Difference between revisions

Browse history interactively

← Previous edit

Content deleted Content added

VisualWikitext

Inline

Latest revision as of 00:17, 19 May 2024

Molecular Biology: Genetics / MCB / COMPBIO Low‑importance

	This article is within the scope of WikiProject Molecular Biology, a collaborative effort to improve the coverage of Molecular Biology on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks.Molecular BiologyWikipedia:WikiProject Molecular BiologyTemplate:WikiProject Molecular BiologyMolecular Biology
Low	This article has been rated as Low-importance on the importance scale.
	This article is supported by the Genetics task force (assessed as Top-importance).
	This article is supported by the Molecular and Cell Biology task force (assessed as High-importance).
	This article is supported by the Computational Biology task force (assessed as High-importance).

Neuroscience Low‑importance

	This article is within the scope of WikiProject Neuroscience, a collaborative effort to improve the coverage of Neuroscience on Wikipedia. If you would like to participate, please visit the project page, where you can join the discussion and see a list of open tasks.NeuroscienceWikipedia:WikiProject NeuroscienceTemplate:WikiProject Neuroscienceneuroscience
Low	This article has been rated as Low-importance on the project's importance scale.

DNA sequencing received a peer review by Wikipedia editors, which is now archived. It may contain ideas you can use to improve this article.

Archives

Archive 1

This page has archives. Sections older than 360 days may be automatically archived by when more than 5 sections are present.

THE REPLICATION AND DETERMINATION OF DNA IN ITS RAW FORM.

If this is possible that a DNA strand can be copied by tRNA mRNA and create a polypeptide sequence, and we know we can replicate it. over the years I have studied this subject and heard the some where someone is mapping the dna sequence of a human being as the dna is all similar obviously not the same or we would all be carbon copies of each other like the bits that make us tall short different eye colour ect. then why do we insist on hacking people open and mending holes in hearts etc if a sample of tissue can be replicated and grown on a mouse back for example how long will it be that it is a simple thing called copy and paste we use it all the time in computers copy the affected area and past a new bit back in this can be put in the form of an injection or hyper spray and as scar tissue is formed over an area of a wound it’s the body’s way of compensating for the loss of information to that area why can’t we do the same.

Media

Adding images to the different ways of sequencing DNA could really help with understanding how they work. EliasMari (talk) 03:00, 26 September 2022 (UTC)[reply]

Wiki Education assignment: Zoology

This article was the subject of a Wiki Education Foundation-supported course assignment, between 30 January 2023 and 30 April 2023. Further details are available on the course page. Student editor(s): Nanalpr (article contribs).

— Assignment last updated by Nanalpr (talk) 02:22, 22 February 2023 (UTC)[reply]

complaint

this section https://en.wikipedia.org/wiki/DNA_sequencing#Microscopy-based_techniques

is sort of clickbaitish: this is a dead method that has never actually worked very well if at all yet posters keep pumping it (not, IMO, hyperbole) editors, can't we do something about this ??? — Preceding unsigned comment added by Cinnamon colbert (talk • contribs) 17:38, 30 December 2023 (UTC)[reply]

complaint

this section https://en.wikipedia.org/wiki/DNA_sequencing#Sample_preparation

is really wierd; it just lists robots but comnpanies like Sage Science, Circulomics,etc et are not mentioned — Preceding unsigned comment added by Cinnamon colbert (talk • contribs) 17:54, 30 December 2023 (UTC)[reply]

@@ Line 1: / Line 1: @@
+{{WikiProject banner shell|class=C|vital=yes|1=
-{{Vital article|topic=Biology|subpage=General|level=5|class=C}}
+{{WikiProject Molecular Biology|importance=Low|genetics=yes|genetics-importance=Top|COMPBIO=yes|COMPBIO-importance=High|MCB=yes|MCB-importance=High}}
-{{WikiProject banner shell|1=
-{{WikiProject Molecular and Cellular Biology |class=C |importance=High |peer-review=yes}}
+{{WikiProject Neuroscience |importance=Low}}
-{{WikiProject Computational Biology |class=C |importance=High}}
-{{WikiProject Genetics |class=C |importance=Top}}
 }}
 {{oldpeerreview|archive=1}}
-{{dashboard.wikiedu.org assignment | course = Wikipedia:Wiki_Ed/Ohio_University/Writing_in_the_Life_Sciences_(Fall) | assignments = [[User:Krusec|Krusec]] | reviewers = [[User:Krusec|Krusec]] }}
 {{User:MiszaBot/config
@@ Line 20: / Line 17: @@
 }}
 {{archivebox|large=no|auto=long}}
-==Whoa, where's the "layman's terms"?==
-Aww, I was hoping to learn how DNA was sequenced, but I reckon if you are an individual that understands "... initiated at a specific site on the template DNA by using a short oligonucleotide 'primer' complementary to the template at that region.", then you probably already know how DNA is sequenced.  Common, there's got to be someone out there that is talented enough with words and biology to make this topic accessible to anyone who tries. :)  -Tom
-:I'll come back later and give it a try. I'll leave a note here so I'll remember  to come back.[[User:Nbauman|Nbauman]] 19:15, 3 November 2006 (UTC)
-This is quandry. Since Wikipedia is an encyclopedia it should be written for senior in high school level. But a senior should know what the four common DNA nucleotides are, that DNA has polarity, what a primer is, and that a primer annealing to a complementary strand could be used to initiate polymerization. But as I write this I can understand. To a molecular biologist DNA sequencing is as straight forward as it gets. To try to explain DNA sequencing and having to explain it all the way down to the basics of DNA polarity is a mind boggling task.
-As a 3rd year molecular biology student at a rather prestigious research university I think I can say that DNA sequencing is not "as straight forward as it gets".  This page could really use a diagram like the one on the [[PCR]] page.  Sure, one might be well off to brush up on [[DNA Replication]] and [[PAGE]] before trying to tackle the intricacies of sequencing, but it might not hurt for the page to be a little clearer on why knowing the length of various segments tells one anything about which nucleotide goes where.  As those in the know, we have a responsibility to share our knowledge with others, and I really think that its attitudes like this that have led whatever majority of americans to believe that the world was created in 6 days.  So lets be helpful, if only for our own sake [[User:ArmyOfFluoride|ArmyOfFluoride]] ([[User talk:ArmyOfFluoride|talk]]) 05:16, 19 March 2009 (UTC)
-No such thing as DNA or not, doesn't matter. Be/can be any no matter what and any can be perfect.  <!-- Template:Unsigned --><small class="autosigned">—&nbsp;Preceding [[Wikipedia:Signatures|unsigned]] comment added by [[User:VunslK|VunslK]] ([[User talk:VunslK#top|talk]] • [[Special:Contributions/VunslK|contribs]]) 07:51, 28 September 2018 (UTC)</small> <!--Autosigned by SineBot-->
 == THE REPLICATION AND DETERMINATION OF DNA IN ITS RAW  FORM. ==
@@ Line 35: / Line 22: @@
 If this is possible that a DNA strand can be copied by tRNA mRNA and create a polypeptide sequence, and we know we can replicate it. over the years I have studied this subject and heard the some where someone is mapping the dna sequence of a human being as the dna is all similar obviously not the same or we would all be carbon copies of each other like the bits that make us tall short different eye colour ect. then why do we insist on hacking people open and mending holes in hearts etc if a sample of tissue can be replicated and grown on a mouse back for example how long will it be that it is a simple thing called copy and paste we use it all the time in computers copy the affected area and past a new bit back in this can be put in the form of an injection or hyper spray and as scar tissue is formed over an area of a wound it’s the body’s way of compensating for the loss of information to that area why can’t we do the same.
+== Media ==
-== About the Sequence of the Pieces of DNA ==
-If DNA is destroyed into millions of pieces before reading, how do they know which piece is after the other?--[[Special:Contributions/95.10.139.121|95.10.139.121]] ([[User talk:95.10.139.121|talk]]) 17:53, 26 May 2015 (UTC)
+Adding images to the different ways of sequencing DNA could really help with understanding how they work. [[User:EliasMari|EliasMari]] ([[User talk:EliasMari|talk]]) 03:00, 26 September 2022 (UTC)
-:This is the problem of [[sequence assembly]], either by mapping the pieces to an existing DNA sequence, or by assembling the fragments without any other information ([[de novo transcriptome assembly]]). --[[User:Amkilpatrick|Amkilpatrick]] ([[User talk:Amkilpatrick|talk]]) 19:00, 27 May 2015 (UTC).
+==Wiki Education assignment: Zoology==
-Don't you need to sequence overlapping fragments ? (that's how I did it 1978). We were happy when we could read more than 100 bp per sequence. [[User:Renebach|Renebach]] ([[User talk:Renebach|talk]]) 13:06, 22 October 2016 (UTC)
+{{dashboard.wikiedu.org assignment | course = Wikipedia:Wiki_Ed/University_of_Puerto_Rico/Zoology_(Spring) | assignments = [[User:Nanalpr|Nanalpr]] | start_date = 2023-01-30 | end_date = 2023-04-30 }}
+<span class="wikied-assignment" style="font-size:85%;">— Assignment last updated by [[User:Nanalpr|Nanalpr]] ([[User talk:Nanalpr|talk]]) 02:22, 22 February 2023 (UTC)</span>
-== Review ==
-=== Proposal for future changes and general review ===
-General suggestions for improvement for this page:
-To begin with I think this page is relatively well put together, but needs some refinement and addition to reflect the current advances in technology.  The introductory section should be improved by the addition of a sentence the introduces the shift from Sanger to next generation sequencing (NGS).  The section '''The four canonical bases''' should probably be removed.
+== complaint ==
-Under '''Use of Sequencing''' the information is cursory and mostly lacking in citations.  Most of these subsections could be vastly improved with an additional sentence explaining methodology and a proper citation (e.g. '''Under Evolutionary Biology''' emphasize the use of comparative genomics to evaluate the similarity and differences of organisms in order to determine their lineage).  This could be cited using a review of phylogenetic studies or the manual for bootstrap.
+this section
+https://en.wikipedia.org/wiki/DNA_sequencing#Microscopy-based_techniques
+is sort of clickbaitish: this is a dead method that has never actually worked very well if at all yet posters keep pumping it (not, IMO, hyperbole)
-Under '''History''' then '''Next Generation sequencing methods''' only the Roche 454 technology is mentioned.  This should be replaced by a general explanation of the advent of NGS and what it has enabled.
+editors, can't we do something about this ???  <!-- Template:Unsigned --><small class="autosigned">—&nbsp;Preceding [[Wikipedia:Signatures|unsigned]] comment added by [[User:Cinnamon colbert|Cinnamon colbert]] ([[User talk:Cinnamon colbert#top|talk]] • [[Special:Contributions/Cinnamon colbert|contribs]]) 17:38, 30 December 2023 (UTC)</small> <!--Autosigned by SineBot-->
-Under '''Advanced methods and de novo sequencing''' Bridge PCR should be removed and the general techniques of NGS (and thus bridge PCR) put under '''Basic Methods'''.
-I love the new addition of '''DNA Nanoball Sequencing'''.  If somebody has the relevant information, the parameters of this method should be added into the table at the top of '''Next-generation sequencing methods'''.
-The '''Sample Preparation''' section, citations are needed and it should be broken into two sections.  One for DNA sample preparation and one for RNA sample preparation and subsequent cDNA conversion.
-Finally, if there is to be a section on '''Read Trimming''' we should link it out to a main article discussing it if possible and a section should be added on '''read assembly''' and/or '''alignment'''
-As a note of my personal background, I am a masters candidate specializing in NGS transcriptomic analysis.  I look forward to feedback and hope some others will take part in the upgrading/updating of this page over the next few weeks.
-[[User:Krusec|Krusec]] ([[User talk:Krusec|talk]]) 02:25, 22 October 2015 (UTC)
-:Hello All, in addition to [[User:Krusec|Krusec]]'s comments, it is a mammoth work but it is probably too technical for your average reader who simply came here to find out - in a nutshell - what is DNA sequencing. (Yes, how long is a piece of string.) However, it would benefit from one section near the beginning that is a very rough diagrammatic overview. I have cobbled together something from other DNA-related pages below but I am certainly no expert. Perhaps someone who is might tailor it, or something similar, for public consumption. We have the materials across Wikipedia; it is only a matter of assembling them. Regards, [[User:William Harris|<span style="color: green">William Harris</span>]] •  [[User talk:William Harris|<span style="color: green">talk • </span>]]  20:40, 18 December 2015 (UTC)
-{{Gallery|title=From specimen to species identification
-|width=150 | height=220|align=centre
-|File:Razboinichya canid.tif
-|The 33,000 year old skull of the [[Origin of the domestic dog#Altai dog - 33,000 BP|"Altai dog"]]
-|File:Eukaryote DNA-en.svg
-|Location of [[nuclear DNA]] within the chromosomes
-|File:ADN animation.gif
-|The structure of part of a DNA [[double helix]]
-|File:Radioactive Fluorescent Seq.jpg
-|An example of the results of automated chain-termination [[DNA sequencing]]
-|File:dna-SNP.svg
-|DNA molecule 1 differs from DNA molecule 2 at a single base-pair location - a [[single-nucleotide polymorphism]]
-|File:Canis lepophagus timeline.png
-|[[Phylogenetic tree]] and timeline towards the dog (Tedford 2009)
-}}
-==much improved==
-from the last time I was here; at least, I don't think there are any really stupid errors (sad to say, there were)
-The whole next gen thing seems a little disorganized and repetitive; perhaps re organizing: by commercial, by date of intro, and non commercial, by date of first real sequence (say 100 bp or bases), and prospsective, no actual sequence data yet
+==complaint==
-if you talk to real sequencers, they will tell you that post sequence bio informatics is half of the work, so that section needs to be expanded - eg, a sequnce isn't any good if you don't know what to do with it  <small class="autosigned">—&nbsp;Preceding [[Wikipedia:Signatures|unsigned]] comment added by [[Special:Contributions/50.245.17.105|50.245.17.105]] ([[User talk:50.245.17.105|talk]]) 15:52, 31 December 2015 (UTC)</small><!-- Template:Unsigned IP --> <!--Autosigned by SineBot-->
+this section
+https://en.wikipedia.org/wiki/DNA_sequencing#Sample_preparation
+is really wierd; it just lists robots but comnpanies like Sage Science, Circulomics,etc et are not mentioned  <!-- Template:Unsigned --><small class="autosigned">—&nbsp;Preceding [[Wikipedia:Signatures|unsigned]] comment added by [[User:Cinnamon colbert|Cinnamon colbert]] ([[User talk:Cinnamon colbert#top|talk]] • [[Special:Contributions/Cinnamon colbert|contribs]]) 17:54, 30 December 2023 (UTC)</small> <!--Autosigned by SineBot-->
-'''Added cPAS section to text and Table to describe technologies'''
-I work for BGI and MGI who build and sell DNA sequencers. I have added text with appropriate references to back up claims. I have not added any text to advantages or disadvantages columns in the Table so as not make any misleading comments. ([[User:AntoBeck|AntoBeck]] ([[User talk:AntoBeck|talk]]) 04:07, 5 July 2018 (UTC))