Excavata: Difference between revisions

Excavata; Temporal range: Neoproterozoic–present Pha. Proterozoic Archean Had.
	Giardia lamblia, a parasitic diplomonad
Scientific classification ; (obsolete as paraphyletic)
Domain:	Eukaryota
(unranked):	Excavata; (Cavalier-Smith), 2002
Phyla and classes
	see text

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Excavata is an extensive and diverse but paraphyletic group of unicellular Eukaryota.^[1]^[2] The group was first suggested by Simpson and Patterson in 1999^[3]^[4] and the name latinized and assigned a rank by Thomas Cavalier-Smith in 2002. It contains a variety of free-living and symbiotic protists, and includes some important parasites of humans such as Giardia and Trichomonas.^[5] Excavates were formerly considered to be included in the now obsolete Protista kingdom.^[6] They were distinguished from other lineages based on electron-microscopic information about how the cells are arranged (they have a distinctive ultrastructural identity).^[4] They are considered to be a basal flagellate lineage.^[7]

On the basis of phylogenomic analyses, the group was shown to contain three widely separated eukaryote groups, the discobids, metamonads, and malawimonads.^[8]^[9]^[10]^[11] A current view of the composition of the excavates is given below, indicating that the group is paraphyletic. Except for some Euglenozoa, all are non-photosynthetic.

Characteristics

Most excavates are unicellular, heterotrophic flagellates. Only some Euglenozoa are photosynthetic. In some (particularly anaerobic intestinal parasites), the mitochondria have been greatly reduced.^[5] Some excavates lack "classical" mitochondria, and are called "amitochondriate", although most retain a mitochondrial organelle in greatly modified form (e.g. a hydrogenosome or mitosome). Among those with mitochondria, the mitochondrial cristae may be tubular, discoidal, or in some cases, laminar. Most excavates have two, four, or more flagella.^[4] Many have a conspicuous ventral feeding groove with a characteristic ultrastructure, supported by microtubules—the "excavated" appearance of this groove giving the organisms their name.^[3]^[6] However, various groups that lack these traits are considered to be derived excavates based on genetic evidence (primarily phylogenetic trees of molecular sequences).^[6]

The Acrasidae slime molds are the only excavates to exhibit limited multicellularity. Like other cellular slime molds, they live most of their life as single cells, but will sometimes assemble into larger clusters.

Proposed group

Excavate relationships were always uncertain, suggesting that they are not a monophyletic group.^[12] Phylogenetic analyses often do not place malawimonads on the same branch as the other Excavata.^[13]

Excavates were thought to include multiple groups:

Kingdom/Superphylum	Included taxa	Representative genera (examples)	Description
Discoba or JEH or Eozoa	Tsukubea	Tsukubamonas
	Euglenozoa	Euglena, Trypanosoma	Many important parasites, one large group with plastids (chloroplasts)
	Heterolobosea (Percolozoa)	Naegleria, Acrasis	Most alternate between flagellate and amoeboid forms
	Jakobea	Jakoba, Reclinomonas	Free-living, sometimes loricate flagellates, with very gene-rich mitochondrial genomes
Metamonada or POD	Preaxostyla	Oxymonads, Trimastix	Amitochondriate flagellates, either free-living (Trimastix, Paratrimastix) or living in the hindguts of insects
	Fornicata	Giardia, Carpediemonas	Amitochondriate, mostly symbiotes and parasites of animals.
	Parabasalia	Trichomonas	Amitochondriate flagellates, generally intestinal commensals of insects. Some human pathogens.
	Anaeramoeba	Anaeramoeba ignava	Anaerobic protists with hydrogenosomes instead of mitochondria.
Neolouka	Malawimonadida	Malawimonas

Discoba or JEH clade

Euglenozoa and Heterolobosea (Percolozoa) or Eozoa (as named by Cavalier-Smith^[14]) appear to be particularly close relatives, and are united by the presence of discoid cristae within the mitochondria (Superphylum Discicristata). A close relationship has been shown between Discicristata and Jakobida,^[15] the latter having tubular cristae like most other protists, and hence were united under the taxon name Discoba, which was proposed for this supposedly monophyletic group.^[1]

Metamonads

Metamonads are unusual in not having classical mitochondria—instead they have hydrogenosomes, mitosomes or uncharacterised organelles. The oxymonad Monocercomonoides is reported to have completely lost homologous organelles. There are competing explanations.^[16]^[17]

Malawimonads

The malawimonads have been proposed to be members of Excavata owing to their typical excavate morphology, and phylogenetic affinity to other excavate groups in some molecular phylogenies. However, their position among eukaryotes remains elusive.^[2]

Ancyromonads

Ancyromonads are small free-living cells with a narrow longitudinal groove down one side of the cell. The ancyromonad groove is not used for "suspension feeding", unlike in "typical excavates" (e.g. malawimonads, jakobids, Trimastix, Carpediemonas, Kiperferlia, etc). Ancyromonads instead capture prokaryotes attached to surfaces. The phylogenetic placement of ancyromonads is poorly understood (in 2020), however some phylogenetic analyses place them as close relatives of malawimonads.^[9]

Evolution

Origin of the Eukaryotes

The conventional explanation for the origin of the Eukaryotes is that a heimdallarchaeian or another Archaea acquired an alphaproteobacterium^[18] as an endosymbiont, and that this became the mitochondrion, the organelle providing oxidative respiration to the eukaryotic cell.^[19]

Caesar al Jewari and Sandra Baldauf argue instead that the Eukaryotes possibly started with an endosymbiosis event of a Deltaproteobacterium or Gammaproteobacterium, accounting for the otherwise unexplained presence of anaerobic bacterial enzymes in Metamonada. The sister of the Preaxostyla within Metamonada represents the rest of the Eukaryotes which acquired an Alphaproteobacterium. In their scenario, the hydrogenosome and mitosome, both conventionally considered "mitochondrion-derived organelles", would predate the mitochondrion, and instead be derived from the earlier symbiotic bacterium.^[17]

Phylogeny

In 2023, using molecular phylogenetic analysis of 186 taxa, Al Jewari and Baldauf proposed a phylogenetic tree with the metamonad Parabasalia as basal Eukaryotes. Discoba and the rest of the Eukaryota appear to have emerged as sister taxon to the Preaxostyla, incorporating a single alphaproteobacterium as mitochondria by endosymbiosis. Thus the Fornicata are more closely related to e.g. animals than to Parabasalia. The rest of the Eukaryotes emerged within the Excavata as sister of the Discoba; as they are within the same clade but are not cladistically considered part of the Excavata yet, the Excavata are in this analysis highly paraphyletic.^[17]

	Heterolobosea

	Euglenozoa and allies

Neokaryotes

Amorphea (inc. animals, fungi)

	SAR

	Archaeplastida (inc. plants)

+ α‑proteobacterium

+ δ/γ‑proteobacterium

"Excavata"

The Anaeramoeba are associated with Parabasalia, but could turn out to be more basal as the root of a tree is often difficult to pinpoint.^[20]

Gallery

Euglena (Euglenozoa: Euglenoida)
Trypanosoma brucei (Euglenozoa: Kinetoplastida)
Bodo sp. (Euglenozoa: Kinetoplastida)
Percolomonas sp. (Percolozoa)
Stephanopogon sp. (Percolozoa)
Stages of Naegleria sp. (Percolozoa: Heterolobosea)
Acrasis rosea (Percolozoa: Heterolobosea)
Jakobids (Jakobida)
Trichomonas vaginalis (Metamonada: Parabasalia)
Retortamonas sp., left (Metamonada: Fornicata: Retortamonadida)
Giardia sp. (Metamonada: Fornicata: Diplomonadida)

References

^ ^a ^b Hampl, Vladimir; Hug, Laura; Leigh, Jessica W.; et al. (2009). "Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic "supergroups"". PNAS. 106 (10): 3859–3864. Bibcode:2009PNAS..106.3859H. doi:10.1073/pnas.0807880106. PMC 2656170. PMID 19237557.
^ ^a ^b Simpson, Alastair G. B.; Inagaki, Yuji; Roger, Andrew J. (2006). "Comprehensive multigene phylogenies of excavate protists reveal the evolutionary positions of "primitive" eukaryotes". Molecular Biology and Evolution. 23 (3): 615–625. doi:10.1093/molbev/msj068. PMID 16308337.
^ ^a ^b Simpson, Alastair G.B.; Patterson, David J. (December 1999). "The ultrastructure of Carpediemonas membranifera (Eukaryota) with reference to the 'excavate hypothesis'". European Journal of Protistology. 35 (4): 353–370. doi:10.1016/S0932-4739(99)80044-3.
^ ^a ^b ^c Simpson, Alastair G. B. (November 2003). "Cytoskeletal organization, phylogenetic affinities and systematics in the contentious taxon Excavata (Eukaryota)". International Journal of Systematic and Evolutionary Microbiology. 53 (6): 1759–1777. doi:10.1099/ijs.0.02578-0. PMID 14657103.
^ ^a ^b Dawkins, Richard; Wong, Yan (2016). The Ancestor's Tale. Houghton Mifflin Harcourt. ISBN 978-0544859937.
^ ^a ^b ^c Cavalier-Smith, Thomas (2002). "The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa". International Journal of Systematic and Evolutionary Microbiology. 52 (2): 297–354. doi:10.1099/00207713-52-2-297. PMID 11931142.
^ Dawson, Scott C.; Paredez, Alexander R. (2013). "Alternative cytoskeletal landscapes: cytoskeletal novelty and evolution in basal excavate protists". Current Opinion in Cell Biology. 25 (1): 134–141. doi:10.1016/j.ceb.2012.11.005. PMC 4927265. PMID 23312067.
^ Burki, Fabien; Roger, Andrew J.; Brown, Matthew W.; et al. (January 2020). "The New Tree of Eukaryotes". Trends in Ecology & Evolution. 35 (1): 43–55. doi:10.1016/j.tree.2019.08.008. PMID 31606140. S2CID 204545629.
^ ^a ^b Brown, Matthew W.; Heiss, Aaron A.; Kamikawa, Ryoma; et al. (2018-01-19). "Phylogenomics Places Orphan Protistan Lineages in a Novel Eukaryotic Super-Group". Genome Biology and Evolution. 10 (2): 427–433. doi:10.1093/gbe/evy014. PMC 5793813. PMID 29360967.
^ Heiss, Aaron A.; Kolisko, Martin; Ekelund, Fleming; et al. (4 April 2018). "Combined morphological and phylogenomic re-examination of malawimonads, a critical taxon for inferring the evolutionary history of eukaryotes". Royal Society Open Science. 5 (4): 171707. Bibcode:2018RSOS....571707H. doi:10.1098/rsos.171707. PMC 5936906. PMID 29765641.
^ Keeling, Patrick J.; Burki, Fabien (19 August 2019). "Progress towards the Tree of Eukaryotes". Current Biology. 29 (16): R808–R817. Bibcode:2019CBio...29.R808K. doi:10.1016/j.cub.2019.07.031. PMID 31430481.
^ Laura Wegener Parfrey; Erika Barbero; Elyse Lasser; Micah Dunthorn; Debashish Bhattacharya; David J Patterson; Laura A Katz (December 2006). "Evaluating support for the current classification of eukaryotic diversity". PLOS Genetics. 2 (12): e220. doi:10.1371/JOURNAL.PGEN.0020220. ISSN 1553-7390. PMC 1713255. PMID 17194223. Wikidata Q21090155.
^ Tice, Alexander K.; Žihala, David; Pánek, Tomáš; et al. (2021). "PhyloFisher: A phylogenomic package for resolving eukaryotic relationships". PLOS Biology. 19 (8): e3001365. doi:10.1371/journal.pbio.3001365. PMC 8345874. PMID 34358228.
^ Cavalier-Smith, Thomas (23 December 2009). "Kingdoms Protozoa and Chromista and the eozoan root of the eukaryotic tree". Biology Letters. 6 (3). The Royal Society: 342–345. doi:10.1098/rsbl.2009.0948. ISSN 1744-9561. PMC 2880060. PMID 20031978.
^ Rodríguez-Ezpeleta, Naiara; Brinkmann, Henner; Burger, Gertraud; et al. (2007). "Toward Resolving the Eukaryotic Tree: The Phylogenetic Positions of Jakobids and Cercozoans". Current Biology. 17 (16): 1420–1425. Bibcode:2007CBio...17.1420R. doi:10.1016/j.cub.2007.07.036. PMID 17689961.
^ Bui, Elisabeth T.; Bradley, Peter J.; Johnson, Patricia J. (3 September 1996). "A common evolutionary origin for mitochondria and hydrogenosomes". Proceedings of the National Academy of Sciences. 93 (18): 9651–9656. Bibcode:1996PNAS...93.9651B. doi:10.1073/pnas.93.18.9651. ISSN 0027-8424. PMC 38483. PMID 8790385.
^ ^a ^b ^c Al Jewari, Caesar; Baldauf, Sandra L. (2023-04-28). "An excavate root for the eukaryote tree of life". Science Advances. 9 (17): eade4973. Bibcode:2023SciA....9E4973A. doi:10.1126/sciadv.ade4973. ISSN 2375-2548. PMC 10146883. PMID 37115919.
^ Tria, F.D.K.; Brueckner, J.; Skejo, J.; Xavier, J.C.; Kapust, N.; Knopp, M.; et al. (7 May 2021). "Gene Duplications Trace Mitochondria to the Onset of Eukaryote Complexity". Genome Biology and Evolution. 13 (5). doi:10.1093/gbe/evab055. PMC 8175051. PMID 33739376.
^ ^a ^b Eme, Laura; Tamarit, Daniel; Caceres, Eva F.; et al. (2023-03-09). "Inference and reconstruction of the heimdallarchaeial ancestry of eukaryotes". Nature. 618 (7967): 992–999. bioRxiv 10.1101/2023.03.07.531504. doi:10.1038/s41586-023-06186-2. PMC 10307638. PMID 37316666.
^ Stairs, Courtney W.; Táborský, Petr; Salomaki, Eric D.; et al. (December 2021). "Anaeramoebae are a divergent lineage of eukaryotes that shed light on the transition from anaerobic mitochondria to hydrogenosomes". Current Biology. 31 (24): 5605–5612.e5. Bibcode:2021CBio...31E5605S. doi:10.1016/j.cub.2021.10.010. ISSN 0960-9822. PMID 34710348. S2CID 240054026.

External links

[pmid19237557-1] Hampl, Vladimir; Hug, Laura; Leigh, Jessica W.; et al. (2009). "Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic "supergroups"". PNAS. 106 (10): 3859–3864. Bibcode:2009PNAS..106.3859H. doi:10.1073/pnas.0807880106. PMC 2656170. PMID 19237557.

[simpson-2] Simpson, Alastair G. B.; Inagaki, Yuji; Roger, Andrew J. (2006). "Comprehensive multigene phylogenies of excavate protists reveal the evolutionary positions of "primitive" eukaryotes". Molecular Biology and Evolution. 23 (3): 615–625. doi:10.1093/molbev/msj068. PMID 16308337.

[Simpson1999-3] Simpson, Alastair G.B.; Patterson, David J. (December 1999). "The ultrastructure of Carpediemonas membranifera (Eukaryota) with reference to the 'excavate hypothesis'". European Journal of Protistology. 35 (4): 353–370. doi:10.1016/S0932-4739(99)80044-3.

[Simpson2003-4] Simpson, Alastair G. B. (November 2003). "Cytoskeletal organization, phylogenetic affinities and systematics in the contentious taxon Excavata (Eukaryota)". International Journal of Systematic and Evolutionary Microbiology. 53 (6): 1759–1777. doi:10.1099/ijs.0.02578-0. PMID 14657103.

[Ancestor-5] Dawkins, Richard; Wong, Yan (2016). The Ancestor's Tale. Houghton Mifflin Harcourt. ISBN 978-0544859937.

[pmid11931142-6] Cavalier-Smith, Thomas (2002). "The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa". International Journal of Systematic and Evolutionary Microbiology. 52 (2): 297–354. doi:10.1099/00207713-52-2-297. PMID 11931142.

[7] Dawson, Scott C.; Paredez, Alexander R. (2013). "Alternative cytoskeletal landscapes: cytoskeletal novelty and evolution in basal excavate protists". Current Opinion in Cell Biology. 25 (1): 134–141. doi:10.1016/j.ceb.2012.11.005. PMC 4927265. PMID 23312067.

[pmid31606140-8] Burki, Fabien; Roger, Andrew J.; Brown, Matthew W.; et al. (January 2020). "The New Tree of Eukaryotes". Trends in Ecology & Evolution. 35 (1): 43–55. doi:10.1016/j.tree.2019.08.008. PMID 31606140. S2CID 204545629.

[Brown_2018-9] Brown, Matthew W.; Heiss, Aaron A.; Kamikawa, Ryoma; et al. (2018-01-19). "Phylogenomics Places Orphan Protistan Lineages in a Novel Eukaryotic Super-Group". Genome Biology and Evolution. 10 (2): 427–433. doi:10.1093/gbe/evy014. PMC 5793813. PMID 29360967.

[10] Heiss, Aaron A.; Kolisko, Martin; Ekelund, Fleming; et al. (4 April 2018). "Combined morphological and phylogenomic re-examination of malawimonads, a critical taxon for inferring the evolutionary history of eukaryotes". Royal Society Open Science. 5 (4): 171707. Bibcode:2018RSOS....571707H. doi:10.1098/rsos.171707. PMC 5936906. PMID 29765641.

[11] Keeling, Patrick J.; Burki, Fabien (19 August 2019). "Progress towards the Tree of Eukaryotes". Current Biology. 29 (16): R808–R817. Bibcode:2019CBio...29.R808K. doi:10.1016/j.cub.2019.07.031. PMID 31430481.

[12] Laura Wegener Parfrey; Erika Barbero; Elyse Lasser; Micah Dunthorn; Debashish Bhattacharya; David J Patterson; Laura A Katz (December 2006). "Evaluating support for the current classification of eukaryotic diversity". PLOS Genetics. 2 (12): e220. doi:10.1371/JOURNAL.PGEN.0020220. ISSN 1553-7390. PMC 1713255. PMID 17194223. Wikidata Q21090155.

[13] Tice, Alexander K.; Žihala, David; Pánek, Tomáš; et al. (2021). "PhyloFisher: A phylogenomic package for resolving eukaryotic relationships". PLOS Biology. 19 (8): e3001365. doi:10.1371/journal.pbio.3001365. PMC 8345874. PMID 34358228.

[Cavalier-Smith_2009-14] Cavalier-Smith, Thomas (23 December 2009). "Kingdoms Protozoa and Chromista and the eozoan root of the eukaryotic tree". Biology Letters. 6 (3). The Royal Society: 342–345. doi:10.1098/rsbl.2009.0948. ISSN 1744-9561. PMC 2880060. PMID 20031978.

[15] Rodríguez-Ezpeleta, Naiara; Brinkmann, Henner; Burger, Gertraud; et al. (2007). "Toward Resolving the Eukaryotic Tree: The Phylogenetic Positions of Jakobids and Cercozoans". Current Biology. 17 (16): 1420–1425. Bibcode:2007CBio...17.1420R. doi:10.1016/j.cub.2007.07.036. PMID 17689961.

[16] Bui, Elisabeth T.; Bradley, Peter J.; Johnson, Patricia J. (3 September 1996). "A common evolutionary origin for mitochondria and hydrogenosomes". Proceedings of the National Academy of Sciences. 93 (18): 9651–9656. Bibcode:1996PNAS...93.9651B. doi:10.1073/pnas.93.18.9651. ISSN 0027-8424. PMC 38483. PMID 8790385.

[Al_Jewari_Baldauf_2023-17] Al Jewari, Caesar; Baldauf, Sandra L. (2023-04-28). "An excavate root for the eukaryote tree of life". Science Advances. 9 (17): eade4973. Bibcode:2023SciA....9E4973A. doi:10.1126/sciadv.ade4973. ISSN 2375-2548. PMC 10146883. PMID 37115919.

[18] Tria, F.D.K.; Brueckner, J.; Skejo, J.; Xavier, J.C.; Kapust, N.; Knopp, M.; et al. (7 May 2021). "Gene Duplications Trace Mitochondria to the Onset of Eukaryote Complexity". Genome Biology and Evolution. 13 (5). doi:10.1093/gbe/evab055. PMC 8175051. PMID 33739376.

[Eme_2023-19] Eme, Laura; Tamarit, Daniel; Caceres, Eva F.; et al. (2023-03-09). "Inference and reconstruction of the heimdallarchaeial ancestry of eukaryotes". Nature. 618 (7967): 992–999. bioRxiv 10.1101/2023.03.07.531504. doi:10.1038/s41586-023-06186-2. PMC 10307638. PMID 37316666.

[20] Stairs, Courtney W.; Táborský, Petr; Salomaki, Eric D.; et al. (December 2021). "Anaeramoebae are a divergent lineage of eukaryotes that shed light on the transition from anaerobic mitochondria to hydrogenosomes". Current Biology. 31 (24): 5605–5612.e5. Bibcode:2021CBio...31E5605S. doi:10.1016/j.cub.2021.10.010. ISSN 0960-9822. PMID 34710348. S2CID 240054026.

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@@ Line 1: / Line 1: @@
 {{short description|Supergroup of unicellular organisms belonging to the domain Eukaryota}}{{For|an explanation of very similar terms|Eukaryota}}{{Automatic taxobox
-| fossil_range = {{long fossil range|Neoproterozoic|present}}
+| fossil_range          = {{long fossil range|Neoproterozoic|present}}
 | classification_status = obsolete as paraphyletic
-| taxon = Excavata
+| taxon                 = Excavata
-| authority = ([[Thomas Cavalier-Smith|Cavalier-Smith]]), 2002
+| authority             = ([[Thomas Cavalier-Smith|Cavalier-Smith]]), 2002
-| image = Giardia lamblia.jpg
+| image                 = Giardia lamblia.jpg
-| image_caption = ''[[Giardia lamblia]]'', a parasitic diplomonad
+| image_caption         = ''[[Giardia lamblia]]'', a parasitic diplomonad
-| subdivision_ranks = Phyla and classes
+| subdivision_ranks     = Phyla and classes
-| subdivision = ''see text''
+| subdivision           = ''see text''
 }}
 [[File:Excavata cell schemes.svg|thumb|Three types of excavate cells. Top: [[Jakobida]], 1-nucleus, 2-anterior flagellum, 3-ventral/posterior flagellum, 4-ventral feeding groove. Middle: [[Euglenozoa]], 1-nucleus, 2-flagellar pocket/reservoir, 3-dorsal/anterior flagellum, 4-ventral/posterior flagellum, 5-cytostome/feeding apparatus. Bottom: [[Metamonada]], 1-anterior flagella, 2-parabasal body, 3-undulating membrane, 4-posterior flagellum, 5-nucleus, 6-[[axostyle]].]]
-'''Excavata''' is an extensive and diverse group of unicellular [[Eukaryota]].<ref>{{cite journal|last1=Hampl|first1=V.|last2=Hug|first2=L.|last3=Leigh|first3=J. W.|last4=Dacks|first4=J. B.|last5=Lang|first5=B. F.|last6=Simpson|first6=A. G. B.|last7=Roger|first7=A. J.|title=Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic "supergroups"|journal=Proceedings of the National Academy of Sciences|year=2009|volume=106|issue=10|pages=3859-864|doi=10.1073/pnas.0807880106|pmid=19237557|pmc=2656170|bibcode=2009PNAS..106.3859H|doi-access=free}}</ref><ref name="pmid19237557">{{cite journal |vauthors=Hampl V, Hug L, Leigh JW |title=Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic "supergroups" |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=106 |issue=10 |pages=3859–64 |year=2009 |pmid=19237557 |doi=10.1073/pnas.0807880106 |pmc=2656170 |bibcode=2009PNAS..106.3859H|display-authors=etal|doi-access=free }}</ref><ref name=simpson>{{Cite journal | doi = 10.1093/molbev/msj068 |year=2006 |author1=Simpson, Ag |author2=Inagaki, Y |author3=Roger, Aj | title = Comprehensive multigene phylogenies of excavate protists reveal the evolutionary positions of "primitive" eukaryotes | volume = 23 | issue = 3 | pages = 615–25 | pmid = 16308337 | journal = Molecular Biology and Evolution | doi-access = free }}</ref> The group was first suggested by Simpson and Patterson in 1999<ref name="Simpson1999">{{Cite journal |last1=Simpson |first1=Alastair G.B. |last2=Patterson |first2=David J. |date=Dec 1999 |title=The ultrastructure of Carpediemonas membranifera (Eukaryota) with reference to the 'excavate hypothesis' |journal=European Journal of Protistology |volume=35 |issue=4 |pages=353–370 |doi=10.1016/S0932-4739(99)80044-3}}</ref><ref name="Simpson2003">{{Cite journal |last=Simpson |first=Alastair G.B. |date=2003-11-01 |title=Cytoskeletal organization, phylogenetic affinities and systematics in the contentious taxon Excavata (Eukaryota) |journal=International Journal of Systematic and Evolutionary Microbiology |volume=53 |issue=6 |pages=1759–1777 |doi=10.1099/ijs.0.02578-0 |doi-access=free |pmid=14657103}}</ref> and the name latinized and assigned a rank by [[Thomas Cavalier-Smith]] in 2002. It contains a variety of free-living and symbiotic protists, and includes some important parasites of humans such as ''[[Giardia]]'' and ''[[Trichomonas]]''.<ref name="Ancestor">{{cite book |title=The Ancestor's Tale |isbn=978-0544859937 |last1=Dawkins  |first1=Richard |last2=Wong |first2=Yan |year=2016 |title-link=The Ancestor's Tale |author1-link=Richard Dawkins }}</ref> Excavates were formerly considered to be included in the now obsolete [[Protists|Protista]] kingdom.<ref name="pmid11931142">{{cite journal|last1=Cavalier-Smith|first1=T|title=The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa.|journal=International Journal of Systematic and Evolutionary Microbiology|year=2002|volume=52|issue=2|pages=297–354|doi=10.1099/00207713-52-2-297|pmid=11931142 }}</ref> They were distinguished from other lineages based on electron-microscopic information about how the cells are arranged (they have a distinctive [[ultrastructural identity]]).<ref name="Simpson2003" /> They are considered to be a basal [[flagellate]] lineage.<ref>{{cite journal|last1=Dawson|first1=Scott C|last2=Paredez|first2=Alexander R|title=Alternative cytoskeletal landscapes: cytoskeletal novelty and evolution in basal excavate protists|journal=Current Opinion in Cell Biology|year=2013|volume=25|issue=1|pages=134–141|doi=10.1016/j.ceb.2012.11.005|pmid=23312067|pmc=4927265}}</ref>
+'''Excavata''' is an extensive and diverse but [[paraphyletic]] group of unicellular [[Eukaryota]].<ref name="pmid19237557">{{cite journal |last1=Hampl |first1=Vladimir |last2=Hug |first2=Laura |last3=Leigh |first3=Jessica W. |last4=Dacks |first4=Joel B. |last5=Lang |first5=B. Franz |last6=Simpson |first6=Alastair G. B. |last7=Roger |first7=Andrew J. |display-authors=3 |title=Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic "supergroups" |journal=PNAS |volume=106 |issue=10 |pages=3859–3864 |year=2009 |pmid=19237557 |doi=10.1073/pnas.0807880106 |pmc=2656170 |bibcode=2009PNAS..106.3859H |doi-access=free  }}</ref><ref name=simpson>{{Cite journal |last1=Simpson |first1=Alastair G. B. |last2=Inagaki |first2=Yuji |last3=Roger |first3=Andrew J. |doi=10.1093/molbev/msj068 |year=2006 |title=Comprehensive multigene phylogenies of excavate protists reveal the evolutionary positions of "primitive" eukaryotes |volume=23 |issue=3 |pages=615–625 |pmid=16308337 |journal=Molecular Biology and Evolution |doi-access=free  }}</ref> The group was first suggested by Simpson and Patterson in 1999<ref name="Simpson1999">{{Cite journal |date=December 1999 |title=The ultrastructure of Carpediemonas membranifera (Eukaryota) with reference to the 'excavate hypothesis' |journal=European Journal of Protistology |volume=35 |issue=4 |pages=353–370 |doi=10.1016/S0932-4739(99)80044-3 |last1=Simpson |first1=Alastair G.B. |last2=Patterson |first2=David J. }}</ref><ref name="Simpson2003">{{Cite journal |last=Simpson |first=Alastair G. B. |date= November 2003 |title=Cytoskeletal organization, phylogenetic affinities and systematics in the contentious taxon Excavata (Eukaryota) |journal=International Journal of Systematic and Evolutionary Microbiology |volume=53 |issue=6 |pages=1759–1777 |doi=10.1099/ijs.0.02578-0 |doi-access=free |pmid=14657103}}</ref> and the name latinized and assigned a rank by [[Thomas Cavalier-Smith]] in 2002. It contains a variety of free-living and symbiotic protists, and includes some important parasites of humans such as ''[[Giardia]]'' and ''[[Trichomonas]]''.<ref name="Ancestor">{{cite book |title=The Ancestor's Tale |isbn=978-0544859937 |last1=Dawkins  |first1=Richard |last2=Wong |first2=Yan |year=2016 |title-link=The Ancestor's Tale |publisher=Houghton Mifflin Harcourt |author1-link=Richard Dawkins }}</ref> Excavates were formerly considered to be included in the now obsolete [[Protists|Protista]] kingdom.<ref name="pmid11931142">{{cite journal|last1=Cavalier-Smith |first1=Thomas |title=The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa.|journal=International Journal of Systematic and Evolutionary Microbiology |year=2002 |volume=52 |issue=2 |pages=297–354 |doi=10.1099/00207713-52-2-297 |pmid=11931142 }}</ref> They were distinguished from other lineages based on electron-microscopic information about how the cells are arranged (they have a distinctive [[ultrastructural identity]]).<ref name="Simpson2003" /> They are considered to be a [[Basal_(phylogenetics)|basal]] [[flagellate]] lineage.<ref>{{cite journal |last1=Dawson |first1=Scott C. |last2=Paredez |first2=Alexander R. |title=Alternative cytoskeletal landscapes: cytoskeletal novelty and evolution in basal excavate protists|journal=Current Opinion in Cell Biology |year=2013 |volume=25 |issue=1 |pages=134–141 |doi=10.1016/j.ceb.2012.11.005 |pmid=23312067 |pmc=4927265}}</ref>
-On the basis of phylogenomic analyses, The group was shown to contain three monophyletic groups (discobids, metamonads, and malawimonads), the relationships among which and indeed their monophyly are uncertain <ref>Burki, F., Roger, A. J., Brown, M.W., Simpson,. A.G.B. 2019.  The new tree of eukaryotes. Trends in Ecology and evolution, 35: 43-55.</ref> <ref>{{cite journal |last1=Brown |first1=MW |last2=Heiss |first2=AA |last3=Kamikawa |first3=R |last4=Inagaki |first4=Y |last5=Yabuki |first5=A |last6=Tice |first6=AK |last7=Shiratori |first7=T |last8=Ishida |first8=KI |last9=Hashimoto |first9=T |last10=Simpson |first10=AGB |last11=Roger |first11=AJ |title=Phylogenomics Places Orphan Protistan Lineages in a Novel Eukaryotic Super-Group. |journal=Genome Biology and Evolution |date=1 February 2018 |volume=10 |issue=2 |pages=427–433 |doi=10.1093/gbe/evy014 |pmid=29360967|pmc=5793813 }}</ref><ref>{{cite journal |last1=Heiss |first1=Aaron A. |last2=Kolisko |first2=Martin |last3=Ekelund |first3=Fleming |last4=Brown |first4=Matthew W. |last5=Roger |first5=Andrew J. |last6=Simpson |first6=Alastair G. B. |title=Combined morphological and phylogenomic re-examination of malawimonads, a critical taxon for inferring the evolutionary history of eukaryotes |journal=Royal Society Open Science |date=4 April 2018 |volume=5 |issue=4 |pages=171707 |doi=10.1098/rsos.171707|pmid=29765641 |pmc=5936906 |bibcode=2018RSOS....571707H }}</ref><ref>{{cite journal |last1=Keeling |first1=PJ |last2=Burki |first2=F |title=Progress towards the Tree of Eukaryotes. |journal=Current Biology |date=19 August 2019 |volume=29 |issue=16 |pages=R808–R817 |doi=10.1016/j.cub.2019.07.031 |pmid=31430481|doi-access=free }}</ref> A current view of the composition of the excavates is given below.  Except for some [[Euglenozoa]],  all are non-photosynthetic.
+On the basis of phylogenomic analyses, the group was shown to contain three widely separated eukaryote groups, the discobids, metamonads, and malawimonads.<ref name="pmid31606140">{{cite journal | last1 = Burki | first1 = Fabien | last2 = Roger | first2 = Andrew J. | last3 = Brown | first3 = Matthew W. | last4 = Simpson | first4 = Alastair G.B. |display-authors=3 | title = The New Tree of Eukaryotes | journal = Trends in Ecology & Evolution | volume = 35 | issue = 1 | pages = 43–55 | date = January 2020 | pmid = 31606140 | doi = 10.1016/j.tree.2019.08.008  | s2cid = 204545629 | doi-access = free }}</ref><ref name = "Brown_2018" /><ref>{{cite journal |last1=Heiss |first1=Aaron A. |last2=Kolisko |first2=Martin |last3=Ekelund |first3=Fleming |last4=Brown |first4=Matthew W. |last5=Roger |first5=Andrew J. |last6=Simpson |first6=Alastair G. B. |display-authors=3 |title=Combined morphological and phylogenomic re-examination of malawimonads, a critical taxon for inferring the evolutionary history of eukaryotes |journal=Royal Society Open Science |date=4 April 2018 |volume=5 |issue=4 |pages=171707 |doi=10.1098/rsos.171707 |pmid=29765641 |pmc=5936906 |bibcode=2018RSOS....571707H }}</ref><ref>{{cite journal |last1=Keeling |first1= Patrick J. |last2=Burki |first2= Fabien |title=Progress towards the Tree of Eukaryotes |journal=Current Biology |date=19 August 2019 |volume=29 |issue=16 |pages=R808–R817 |doi=10.1016/j.cub.2019.07.031 |pmid=31430481 |doi-access=free |bibcode= 2019CBio...29.R808K }}</ref> A current view of the composition of the excavates is given below, indicating that the group is paraphyletic. Except for some [[Euglenozoa]],  all are non-[[Photosynthesis|photosynthetic]].
 ==Characteristics==
@@ Line 29: / Line 27: @@
 {{anchor|Discoba}}
-Excavate relationships were always uncertain, suggesting that they are not a [[monophyletic]] group.<ref>{{cite Q|Q21090155}}</ref> The malawimonads often do not branch with the other Excavata.<ref>{{Cite journal |last1=Tice |first1=Alexander K. |last2=Žihala |first2=David |last3=Pánek |first3=Tomáš |last4=Jones |first4=Robert E. |last5=Salomaki |first5=Eric D. |last6=Nenarokov |first6=Serafim |last7=Burki |first7=Fabien |last8=Eliáš |first8=Marek |last9=Eme |first9=Laura |last10=Roger |first10=Andrew J. |last11=Rokas |first11=Antonis |last12=Shen |first12=Xing-Xing |last13=Strassert |first13=Jürgen F. H. |last14=Kolísko |first14=Martin |last15=Brown |first15=Matthew W. |date=2021 |title=PhyloFisher: A phylogenomic package for resolving eukaryotic relationships |journal=PLOS Biology |volume=19 |issue=8 |pages=e3001365 |doi=10.1371/journal.pbio.3001365 |pmc=8345874 |pmid=34358228}}</ref>
+Excavate relationships were always uncertain, suggesting that they are not a [[monophyletic]] group.<ref>{{cite Q|Q21090155|doi-access=free}}</ref> Phylogenetic analyses often do not place malawimonads on the same branch as the other Excavata.<ref>{{Cite journal |last1=Tice |first1=Alexander K. |last2=Žihala |first2=David |last3=Pánek |first3=Tomáš |last4=Jones |first4=Robert E. |last5=Salomaki |first5=Eric D. |last6=Nenarokov |first6=Serafim |last7=Burki |first7=Fabien |last8=Eliáš |first8=Marek |last9=Eme |first9=Laura |last10=Roger |first10=Andrew J. |last11=Rokas |first11=Antonis |last12=Shen |first12=Xing-Xing |last13=Strassert |first13=Jürgen F. H. |last14=Kolísko |first14=Martin |last15=Brown |first15=Matthew W. | display-authors = 3 |date=2021 |title=PhyloFisher: A phylogenomic package for resolving eukaryotic relationships |journal=PLOS Biology |volume=19 |issue=8 |pages=e3001365 |doi=10.1371/journal.pbio.3001365 |pmc=8345874 |pmid=34358228 |doi-access=free }}</ref>
 Excavates were thought to include multiple groups:
@@ Line 46: / Line 44: @@
 |[[Jakobea]] || ''[[Jakoba]]'', ''[[Reclinomonas]]'' || Free-living, sometimes loricate flagellates, with very gene-rich mitochondrial genomes
 |-
-|rowspan=3|[[Metamonada]]&nbsp;or&nbsp;POD||[[Preaxostyla]] || [[Oxymonads]], ''[[Trimastix]]'' || [[Amitochondriate]] flagellates, either free-living (''[[Trimastix]]'', ''Paratrimastix'') or living in the hindguts of insects
+|rowspan=4|[[Metamonada]]&nbsp;or&nbsp;POD||[[Preaxostyla]] || [[Oxymonads]], ''[[Trimastix]]'' || [[Amitochondriate]] flagellates, either free-living (''[[Trimastix]]'', ''Paratrimastix'') or living in the hindguts of insects
 |-
 |[[Fornicata]]|| ''[[Giardia]]'', ''[[Carpediemonas]]'' || Amitochondriate, mostly symbiotes and parasites of animals.
 |-
 |[[Parabasalia]]|| ''[[Trichomonas]]'' || Amitochondriate flagellates, generally intestinal [[Commensalism|commensals]] of insects. Some human pathogens.
+|-
+|[[Anaeramoeba]]|| ''Anaeramoeba ignava'' ||Anaerobic protists with [[hydrogenosome]]s instead of mitochondria.
 |-
 |[[Neolouka]]
@@ Line 59: / Line 59: @@
 ===Discoba or JEH clade===
-Euglenozoa and Heterolobosea (Percolozoa) or Eozoa (Cavalier-Smith) appear to be particularly close relatives, and are united by the presence of discoid [[crista]]e within the [[mitochondrion|mitochondria]] (Superphylum [[Discicristata]]). A close relationship has been shown between Discicristata and [[Jakobida]],<ref>{{cite journal | title=Toward Resolving the Eukaryotic Tree: The Phylogenetic Positions of Jakobids and Cercozoans | author=Naiara Rodríguez-Ezpeleta | author2=Henner Brinkmann | author3=Gertraud Burger | author4=Andrew J. Roger | author5=Michael W. Gray | author6=Hervé Philippe | author7=B. Franz Lang | name-list-style=amp| doi=10.1016/j.cub.2007.07.036| journal=Curr. Biol. | year=2007 | volume=17 | issue=16 | pages=1420–1425 | pmid=17689961| doi-access=free }}</ref> the latter having tubular cristae like most other protists, and hence were united under the taxon name '''Discoba''', which was proposed for this apparently monophyletic group.<ref name="pmid19237557" />
+Euglenozoa and Heterolobosea (Percolozoa) or Eozoa (as named by Cavalier-Smith<ref name="Cavalier-Smith 2009">{{cite journal |last=Cavalier-Smith |first=Thomas |title=Kingdoms Protozoa and Chromista and the eozoan root of the eukaryotic tree |journal=Biology Letters |publisher=The Royal Society |volume=6 |issue=3 |date=23 December 2009 |issn=1744-9561 |doi=10.1098/rsbl.2009.0948 |pages=342–345|pmid=20031978 |pmc=2880060 }}</ref>) appear to be particularly close relatives, and are united by the presence of discoid [[crista]]e within the [[mitochondrion|mitochondria]] (Superphylum [[Discicristata]]). A close relationship has been shown between Discicristata and [[Jakobida]],<ref>{{cite journal |title=Toward Resolving the Eukaryotic Tree: The Phylogenetic Positions of Jakobids and Cercozoans |last1=Rodríguez-Ezpeleta |first1=Naiara |last2=Brinkmann |first2=Henner |last3=Burger |first3=Gertraud |last4=Roger |first4=Andrew J. |last5=Gray |first5=Michael W. |last6=Philippe |first6=Hervé |last7=Lang |first7=B. Franz |display-authors=3 |doi=10.1016/j.cub.2007.07.036 |journal=Current Biology |year=2007 |volume=17 |issue=16 |pages=1420–1425 |pmid=17689961 |doi-access=free  |bibcode=2007CBio...17.1420R }}</ref> the latter having tubular cristae like most other protists, and hence were united under the taxon name '''Discoba''', which was proposed for this supposedly monophyletic group.<ref name="pmid19237557" />
 ===Metamonads===
-Metamonads are unusual in not having classical mitochondria—instead they have [[hydrogenosome]]s, [[mitosome]]s or uncharacterised organelles. The oxymonad ''[[Monocercomonoides]]'' is reported to have completely lost homologous organelles. There are competing explanations.<ref>{{Cite journal |last=Bui |first=E T |last2=Bradley |first2=P J |last3=Johnson |first3=P J |date=1996-09-03 |title=A common evolutionary origin for mitochondria and hydrogenosomes. |url=https://pnas.org/doi/full/10.1073/pnas.93.18.9651 |journal=Proceedings of the National Academy of Sciences |volume=93 |issue=18 |pages=9651–9656 |doi=10.1073/pnas.93.18.9651 |issn=0027-8424 |pmc=38483 |pmid=8790385}}</ref><ref name="Al Jewari Baldauf 2023" />
+Metamonads are unusual in not having classical mitochondria—instead they have [[hydrogenosome]]s, [[mitosome]]s or uncharacterised organelles. The oxymonad ''[[Monocercomonoides]]'' is reported to have completely lost homologous organelles. There are competing explanations.<ref>{{Cite journal |date=3 September 1996 |title=A common evolutionary origin for mitochondria and hydrogenosomes |journal=Proceedings of the National Academy of Sciences |volume=93 |issue=18 |pages=9651–9656 |doi=10.1073/pnas.93.18.9651 |issn=0027-8424 |pmc=38483 |pmid=8790385 |bibcode=1996PNAS...93.9651B |doi-access=free |last1=Bui |first1= Elisabeth T. |last2=Bradley |first2=Peter J. |last3=Johnson |first3=Patricia J. }}</ref><ref name="Al Jewari Baldauf 2023" />
 ===Malawimonads===
@@ Line 71: / Line 71: @@
 ===Ancyromonads===
-[[Ancyromonadida|Ancyromonads]] are small free-living cells with a narrow longitudinal groove down one side of the cell.  The ancyromonad groove is not used for "suspension feeding", unlike in "typical excavates" (e.g. malawimonads, jakobids, ''Trimastix'', ''Carpediemonas'', ''Kiperferlia'', etc).  Ancyromonads instead capture prokaryotes attached to surfaces. The phylogenetic placement of ancyromonads is poorly understood (in 2020), however some phylogenetic analyses place them as close relatives of malawimonads.<ref>{{Cite journal|last1=Brown|first1=Matthew W|last2=Heiss|first2=Aaron A|last3=Kamikawa |first3=Ryoma |last4=Inagaki |first4=Yuji|last5=Yabuki|first5=Akinori|last6=Tice|first6=Alexander K|last7=Shiratori |first7=Takashi|last8=Ishida|first8=Ken-Ichiro|last9=Hashimoto|first9=Tetsuo|last10=Simpson|first10=Alastair |last11=Roger |first11=Andrew|date=2018-01-19|title=Phylogenomics Places Orphan Protistan Lineages in a Novel Eukaryotic Super-Group |journal=Genome Biology and Evolution|volume=10|issue=2|pages=427–433|doi=10.1093/gbe/evy014|pmc=5793813|pmid=29360967}}</ref>
+[[Ancyromonadida|Ancyromonads]] are small free-living cells with a narrow longitudinal groove down one side of the cell.  The ancyromonad groove is not used for "suspension feeding", unlike in "typical excavates" (e.g. malawimonads, jakobids, ''Trimastix'', ''Carpediemonas'', ''Kiperferlia'', etc).  Ancyromonads instead capture prokaryotes attached to surfaces. The phylogenetic placement of ancyromonads is poorly understood (in 2020), however some phylogenetic analyses place them as close relatives of malawimonads.<ref name = "Brown_2018">{{Cite journal |last1=Brown |first1=Matthew W. |last2=Heiss |first2=Aaron A. |last3=Kamikawa |first3=Ryoma |last4=Inagaki |first4=Yuji |last5=Yabuki |first5=Akinori |last6=Tice |first6=Alexander K. |last7=Shiratori |first7=Takash i|last8=Ishida |first8=Ken-Ichiro |last9=Hashimoto |first9=Tetsuo |last10=Simpson |first10=Alastair |last11=Roger |first11=Andrew |display-authors=3 |date=2018-01-19|title=Phylogenomics Places Orphan Protistan Lineages in a Novel Eukaryotic Super-Group |journal=Genome Biology and Evolution |volume=10 |issue=2 |pages=427–433 |doi=10.1093/gbe/evy014 |pmc=5793813 |pmid=29360967}}</ref>
 == Evolution ==
 === Origin of the Eukaryotes ===
 {{further|Eukaryogenesis}}
-The conventional explanation for the origin of the Eukaryotes is that a [[heimdallarchaeia]]n<ref name="Eme 2023">{{Cite web |last=Eme |first=Laura |last2=Tamarit |first2=Daniel |last3=Caceres |first3=Eva F. |last4=Stairs |first4=Courtney W. |last5=Anda |first5=Valerie De |last6=Schön |first6=Max E. |last7=Seitz |first7=Kiley W. |last8=Dombrowski |first8=Nina |last9=Lewis |first9=William H. |last10=Homa |first10=Felix |last11=Saw |first11=Jimmy H. |last12=Lombard |first12=Jonathan |last13=Nunoura |first13=Takuro |last14=Li |first14=Wen-Jun |last15=Hua |first15=Zheng-Shuang |display-authors=3 |date=2023-03-09 |title=Inference and reconstruction of the heimdallarchaeial ancestry of eukaryotes |website=bioRxiv |url=https://www.biorxiv.org/content/10.1101/2023.03.07.531504v1 |pages=2023.03.07.531504 |doi=10.1101/2023.03.07.531504v1}}</ref> or another Archaea acquired an [[Alphaproteobacteria|alphaproteobacterium]] as an [[Endosymbiont hypothesis|endosymbiont]], and that this became the [[mitochondrion]], the [[organelle]] providing [[Citric acid cycle|oxidative respiration]] to the eukaryotic cell. Caesar al Jewari and Sandra Baldauf argue instead that the Eukaryotes possibly started with an endosymbiosis event of a deltaproteobacterium or gammaproteobacterium, accounting for the otherwise unexplained presence of anaerobic bacterial enzymes in metamonada in this scenario. The sister of the Preaxostyla within Metamonada represents the rest of the Eukaryotes which acquired a alphaproteobacterium.<ref name="Al Jewari Baldauf 2023">{{Cite journal |last=Al Jewari |first=Caesar |last2=Baldauf |first2=Sandra L. |date=2023-04-28 |title=An excavate root for the eukaryote tree of life |url=https://www.science.org/doi/10.1126/sciadv.ade4973 |journal=Science Advances |volume=9 |issue=17 |pages=eade4973 |doi=10.1126/sciadv.ade4973 |issn=2375-2548 |pmc=10146883 |pmid=37115919}}</ref>
+The conventional explanation for the origin of the Eukaryotes is that a [[heimdallarchaeia]]n or another Archaea acquired an [[Alphaproteobacteria|alphaproteobacterium]]<ref>{{cite journal |last1=Tria |first1=F.D.K. |last2=Brueckner |first2=J. |last3=Skejo |first3=J. |last4=Xavier |first4=J.C. |last5=Kapust |first5=N. |last6=Knopp |first6=M. |last7=Wimmer |first7=J.L.E. |last8=Nagies |first8=F.S.P. |last9=Zimorski |first9=V. |last10=Gould |first10=S.B. |last11=Garg |first11=S.G. |last12=Martin |first12=W.F. |display-authors=6 |title=Gene Duplications Trace Mitochondria to the Onset of Eukaryote Complexity. |journal=Genome Biology and Evolution |date=7 May 2021 |volume=13 |issue=5 |doi=10.1093/gbe/evab055 |pmid=33739376|pmc=8175051 }}</ref> as an [[Endosymbiont hypothesis|endosymbiont]], and that this became the [[mitochondrion]], the [[organelle]] providing [[Citric acid cycle|oxidative respiration]] to the eukaryotic cell.<ref name="Eme 2023">{{Cite journal |last1=Eme |first1=Laura |last2=Tamarit |first2=Daniel |last3=Caceres |first3=Eva F. |last4=Stairs |first4=Courtney W. |last5=Anda |first5=Valerie De |last6=Schön |first6=Max E. |last7=Seitz |first7=Kiley W. |last8=Dombrowski |first8=Nina |last9=Lewis |first9=William H. |last10=Homa |first10=Felix |last11=Saw |first11=Jimmy H. |last12=Lombard |first12=Jonathan |last13=Nunoura |first13=Takuro |last14=Li |first14=Wen-Jun |last15=Hua |first15=Zheng-Shuang |display-authors=3 |date=2023-03-09 |title=Inference and reconstruction of the heimdallarchaeial ancestry of eukaryotes |journal=Nature |volume=618 |issue=7967 |pages=992–999 |doi=10.1038/s41586-023-06186-2|biorxiv=10.1101/2023.03.07.531504|pmid=37316666 |pmc=10307638 }}</ref>
+Caesar al Jewari and Sandra Baldauf argue instead that the Eukaryotes possibly started with an endosymbiosis event of a [[Deltaproteobacterium]] or [[Gammaproteobacterium]], accounting for the otherwise unexplained presence of anaerobic bacterial enzymes in Metamonada. The sister of the Preaxostyla within Metamonada represents the rest of the Eukaryotes which acquired an Alphaproteobacterium. In their scenario, the [[hydrogenosome]] and [[mitosome]], both conventionally considered "mitochondrion-derived organelles", would predate the mitochondrion, and instead be derived from the earlier symbiotic bacterium.<ref name="Al Jewari Baldauf 2023">{{Cite journal |last1=Al Jewari |first1=Caesar |last2=Baldauf |first2=Sandra L. |date=2023-04-28 |title=An excavate root for the eukaryote tree of life |journal=Science Advances |volume=9 |issue=17 |pages=eade4973 |doi=10.1126/sciadv.ade4973 |issn=2375-2548 |pmc=10146883 |pmid=37115919 |bibcode=2023SciA....9E4973A }}</ref>
 === Phylogeny ===
-In 2023, using molecular phylogenetic analysis of 186 taxa, Al Jewari and Baldauf proposed a phylogenetic tree with the metamonad Parabasalia as basal Eukaryotes. Discoba and the rest of the Eukaryota appear to have emerged as [[Sister group|sister taxon]] to the Preaxostyla, incorporating a single alphaproteobacterium as mitochondria by endosymbiosis. Thus the Fornicata are more closely related to e.g. animals than to Parabasalia. The rest of the Eukaryotes emerged within the Excavata as sister of the Discoba.<ref name="Al Jewari Baldauf 2023"/>
+In 2023, using molecular phylogenetic analysis of 186 taxa, Al Jewari and Baldauf proposed a phylogenetic tree with the metamonad Parabasalia as basal Eukaryotes. Discoba and the rest of the Eukaryota appear to have emerged as [[Sister group|sister taxon]] to the Preaxostyla, incorporating a single alphaproteobacterium as mitochondria by endosymbiosis. Thus the Fornicata are more closely related to e.g. animals than to Parabasalia. The rest of the Eukaryotes emerged within the Excavata as sister of the Discoba; as they are within the same clade but are not [[cladistically]] considered part of the Excavata yet, the Excavata are in this analysis highly paraphyletic.<ref name="Al Jewari Baldauf 2023"/>
 {{clade
@@ Line 90: / Line 91: @@
 |1=[[Heimdallarchaeia|Hodarchaeales]]<ref name="Eme 2023"/>
 |label2=[[Eukaryota]]
-|sublabel2=<small>(+[[proteobacterium]])</small>
+|sublabel2=+ &delta;/&gamma;-proteobacterium
 |2={{clade
    |1=[[Parabasalid|Parabasalia]] |barbegin1=red
@@ Line 97: / Line 98: @@
       |2={{clade
          |1=[[Preaxostyla]] |bar1=red
-         |sublabel2=<small>(+[[Alphaproteobacteria|alphaproteobacterium]])</small>
+         |sublabel2=+ α-proteobacterium
          |2={{clade
             |label1=[[Discoba]] |barend1=red
@@ Line 112: / Line 113: @@
                |2={{clade
                   |1=[[SAR supergroup|SAR]]
-|sublabel2=<small>(+[[cyanobacterium]])</small>
                   |2=[[Archaeplastida]] (inc. plants)
                   }}
@@ Line 121: / Line 121: @@
    }}}}
 }}
+The [[Anaeramoeba]] are associated with Parabasalia, but could turn out to be more basal as the root of a tree is often difficult to pinpoint.<ref>{{Cite journal |last1=Stairs |first1=Courtney W. |last2=Táborský |first2=Petr |last3=Salomaki |first3=Eric D. |last4=Kolisko |first4=Martin |last5=Pánek |first5=Tomáš |last6=Eme |first6=Laura |last7=Hradilová |first7=Miluše |last8=Vlček |first8=Čestmír |last9=Jerlström-Hultqvist |first9=Jon |last10=Roger |first10=Andrew J. |last11=Čepička |first11=Ivan |display-authors=3 |date=December 2021 |title=Anaeramoebae are a divergent lineage of eukaryotes that shed light on the transition from anaerobic mitochondria to hydrogenosomes |journal=Current Biology |volume=31 |issue=24 |pages=5605–5612.e5 |doi=10.1016/j.cub.2021.10.010 |pmid=34710348 |s2cid=240054026 |issn=0960-9822|doi-access=free |bibcode=2021CBio...31E5605S }}</ref>
 == See also ==

Excavata Temporal range: Neoproterozoic–present Pha. Proterozoic Archean Had.

Giardia lamblia, a parasitic diplomonad
Scientific classification (obsolete as paraphyletic)
Domain:	Eukaryota
(unranked):	Excavata (Cavalier-Smith), 2002
Phyla and classes
see text