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TRIzol is a widely used^[1] chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987.^[2]

TRIzol is the brand name of guanidinium thiocyanate from the Ambion part of Life Technologies,^[3] and Tri-Reagent is the brand name from MRC,^[4] which was founded by Chomczynski.

Uses in extraction

The correct name of the method is guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield.^[5]^[6] An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.

DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct-zol Miniprep kit by Zymo Research.^[7] This method eliminates the use of Chloroform and 1-bromo-3-chloropropane completely, bypassing phase-separation and precipitation steps.^[8]

TRIzol is light-sensitive and is often stored in a dark-colored, glass container covered in foil. It is stored at room temperature.

When used, it resembles cough syrup, bright pink. The smell of the phenol is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.

Hazards

Vigilant caution should be taken while using TRIzol (due to the phenol and chloroform).

TRIzol is labeled as acute oral, dermal, and inhalation toxicity besides skin corrosion/irritation in the manufacturer MDS.^[9]

Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns, permanent scarring and kidney failure.

Experiments should be performed under a chemical hood, with lab coat, nitrile gloves and a plastic apron. ^[10]^[11]

TRIzol waste should never be mixed with bleach or acids: the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases.

References

^ "Total RNA isolation: Trizol vs RNeasy". www.bioastrum.com. Retrieved 2021-08-18.
^ Chomczynski, P; Sacchi, N (April 1987). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Anal. Biochem. 162 (1): 156–9. doi:10.1016/0003-2697(87)90021-2. PMID 2440339.
^ Life Technologies
^ Molecular Research Center, Inc.
^ "RNA isolation" (PDF). Cell Analysis Facility. Archived from the original (PDF) on 2021-08-18. Retrieved 2021-08-18.
^ "Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database". brd.nci.nih.gov. Retrieved 2021-08-18.
^ "Direct-zol RNA Microprep Kits".
^ "Nucleic acid purification".
^ "TRIZOl MDS" (PDF).
^ Material Safety data sheet from Life technologies. Archived 2008-07-21 at the Wayback Machine
^ Material Safety data sheet from MRC Archived 2008-12-28 at the Wayback Machine

External links

RNA extraction using trizol protocol on OpenWetWare

[1] "Total RNA isolation: Trizol vs RNeasy". www.bioastrum.com. Retrieved 2021-08-18.

[2] Chomczynski, P; Sacchi, N (April 1987). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Anal. Biochem. 162 (1): 156–9. doi:10.1016/0003-2697(87)90021-2. PMID 2440339.

[3] Life Technologies

[4] Molecular Research Center, Inc.

[5] "RNA isolation" (PDF). Cell Analysis Facility. Archived from the original (PDF) on 2021-08-18. Retrieved 2021-08-18.

[6] "Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database". brd.nci.nih.gov. Retrieved 2021-08-18.

[7] "Direct-zol RNA Microprep Kits".

[8] "Nucleic acid purification".

[9] "TRIZOl MDS" (PDF).

[10] Material Safety data sheet from Life technologies. Archived 2008-07-21 at the Wayback Machine

[11] Material Safety data sheet from MRC Archived 2008-12-28 at the Wayback Machine

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@@ Line 1: / Line 1: @@
+{{Short description|Chemical solution used in RNA/DNA/protein extraction}}
-[[File:TRIzol reagent.JPG|thumb|right|300px|TRIzol reagent contains [[Guanidinium thiocyanate]] and [[Phenol]].]]
+[[File:TRIzol reagent.JPG|thumb|right|300px|TRIzol reagent contains [[guanidinium thiocyanate]] and [[phenol]].]]
-'''TRIzol''' is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from [[Piotr Chomczyński]] and Sacchi, N. in 1987.<ref>[http://www.ncbi.nlm.nih.gov/pubmed/2440339 Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156-9.]</ref>
+'''TRIzol''' is a widely used<ref>{{Cite web|title=Total RNA isolation: Trizol vs RNeasy|url=http://www.bioastrum.com/home/content/total-rna-isolation-trizol-vs-rneasy|access-date=2021-08-18|website=www.bioastrum.com|language=en}}</ref> chemical solution used in the extraction of [[DNA]], [[RNA]], and [[proteins]] from cells. The solution was initially used and published by [[Piotr Chomczyński]] and [[Nicoletta Sacchi]] in 1987.<ref>{{cite journal| pmid=2440339 | doi=10.1016/0003-2697(87)90021-2 | volume=162 | title=Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction | date=April 1987 | journal=Anal. Biochem. | pages=156–9 | last1 = Chomczynski | first1 = P | last2 = Sacchi | first2 = N| issue=1 }}</ref>
-TRIzol is the brand name of the product from Ambion part of [[Life Technologies (Thermo Fisher Scientific)|Life Technologies]],<ref>[http://www.invitrogen.com/site/us/en/home/brands/Product-Brand/Trizol.html Life Technologies]</ref> and the brand name from MRC,<ref>[http://www.mrcgene.com/ Molecular Research Center, Inc.]</ref> which was founded by Chomczynski, is TRI Reagent.
+TRIzol is the brand name of [[guanidinium thiocyanate]] from the Ambion part of [[Life Technologies (Thermo Fisher Scientific)|Life Technologies]],<ref>[http://www.invitrogen.com/site/us/en/home/brands/Product-Brand/Trizol.html Life Technologies]</ref> and Tri-Reagent is the brand name from MRC,<ref>[http://www.mrcgene.com/ Molecular Research Center, Inc.]</ref> which was founded by Chomczynski.
+==Uses in extraction==
-The correct name of the method is [[Guanidinium thiocyanate-phenol-chloroform extraction]]. The use of TRIzol can result in DNA and RNA yields comparable to other extraction methods. An alternative method for [[RNA extraction]] is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.
+The correct name of the method is [[guanidinium thiocyanate-phenol-chloroform extraction]]. The use of TRIzol can result in DNA yields comparable to other extraction methods, and it leads to >50% bigger RNA yield.<ref>{{Cite web |title=RNA isolation |url=https://depts.washington.edu/flowlab/Cell%20Analysis%20Facility/Cell%20sorting%20for%20RNA%20isolation.pdf |url-status=dead |website=Cell Analysis Facility |access-date=2021-08-18 |archive-date=2021-08-18 |archive-url=https://web.archive.org/web/20210818142832/https://depts.washington.edu/flowlab/Cell%20Analysis%20Facility/Cell%20sorting%20for%20RNA%20isolation.pdf }}</ref><ref>{{Cite web|title=Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. - Biospecimen Research Database|url=https://brd.nci.nih.gov/brd/paper/cardiovasc-pathol/2015/evaluation-of-optimal-rna-extraction-method-from-human-carotid/123390#:~:text=RNA%20yields%20were%20approximately%204.5-fold%20higher%20from%20snap-frozen,numbers%20(RIN)%20were%20significantly%20lower%20than%20using%20RNEasy.|access-date=2021-08-18|website=brd.nci.nih.gov}}</ref> An alternative method for [[RNA extraction]] is phenol extraction and TCA/acetone precipitation. Chloroform should be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.
+DNA and RNA from TRIzol and TRI reagent can also be extracted using the Direct-zol Miniprep kit by [[Zymo Research]].<ref>{{Cite web|url=https://www.zymoresearch.com/collections/direct-zol-rna-kits/products/direct-zol-rna-microprep-kits|title=Direct-zol RNA Microprep Kits}}</ref> This method eliminates the use of Chloroform and 1-bromo-3-chloropropane completely, bypassing phase-separation and precipitation steps.<ref>{{Cite web|url=https://patents.google.com/patent/EP2770056A1|title=Nucleic acid purification}}</ref>
-TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.
+TRIzol is light-sensitive and is often stored in a dark-colored, glass container covered in foil. It is stored at room temperature.
 When used, it resembles cough syrup, bright pink. The smell of the [[phenol]] is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
 == Hazards ==
-Caution should be taken while using TRIzol (specifically [[phenol]]) and [[Chloroform]].
+Vigilant caution should be taken while using TRIzol (due to the [[phenol]] and [[chloroform]]).
+TRIzol is labeled as acute oral, dermal, and inhalation toxicity besides skin corrosion/irritation in the manufacturer MDS.<ref>{{Cite web|title=TRIZOl MDS|url=http://tools.thermofisher.com/content/sfs/msds/2012/15596026_MTR-NALT_EN.pdf}}</ref>
-Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns and permanent scarring. A lab coat, gloves and a plastic apron are recommended <ref>[http://cellbiology.med.unsw.edu.au/units/lab/msds/Trizol.pdf Material Safety data sheet from Life technologies.]</ref><ref>[http://www.mrcgene.com/msdsrna.htm Material Safety data sheet from MRC]</ref>
+Exposure to TRIzol can be a serious health hazard. Exposure can lead to serious chemical burns, permanent scarring and kidney failure.
+Experiments should be performed under a chemical hood, with lab coat, nitrile gloves and a plastic apron. <ref>[http://cellbiology.med.unsw.edu.au/units/lab/msds/Trizol.pdf Material Safety data sheet from Life technologies.] {{Webarchive|url=https://web.archive.org/web/20080721185232/http://cellbiology.med.unsw.edu.au/units/lab/msds/Trizol.pdf |date=2008-07-21 }}</ref><ref>[http://www.mrcgene.com/msdsrna.htm Material Safety data sheet from MRC] {{Webarchive|url=https://web.archive.org/web/20081228194341/http://mrcgene.com/msdsrna.htm |date=2008-12-28 }}</ref>
+TRIzol waste should never be mixed with bleach or acids: the guanidinium thiocyanate in TRIzol reacts to form highly toxic gases.
 == References ==
@@ Line 24: / Line 34: @@
 [[Category:Molecular biology]]
 [[Category:Biochemistry methods]]
-{{product-stub}}