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'''Nisin''' is a polycyclic antibacterial [[peptide]] produced by the [[bacterium]] ''[[Lactococcus lactis]]'' that is used as a food [[preservative]]. It has 34 [[amino acid]] residues, including the uncommon amino acids [[lanthionine]] (Lan), [[methyllanthionine]] (MeLan), [[didehydroalanine]] (Dha), and [[didehydroaminobutyric acid]] (Dhb). These unusual amino acids are introduced by [[posttranslational modification]] of the precursor peptide. In these reactions a [[ribosome|ribosomally]] synthesized 57-mer is converted to the final peptide. The unsaturated amino acids originate from [[serine]] and [[threonine]], and the enzyme-catalysed addition of [[cysteine]] residues to the didehydro amino acids result in the multiple (5) [[thioether]] bridges. |
'''Nisin''' is a polycyclic antibacterial [[peptide]] produced by the [[bacterium]] ''[[Lactococcus lactis]]'' that is used as a food [[preservative]]. It has 34 [[amino acid]] residues, including the uncommon amino acids [[lanthionine]] (Lan), [[methyllanthionine]] (MeLan), [[didehydroalanine]] (Dha), and [[didehydroaminobutyric acid]] (Dhb). These unusual amino acids are introduced by [[posttranslational modification]] of the precursor peptide.<ref>{{cite journal | pmid=2493449 | date=1989 | last1=Kaletta | first1=C. | last2=Entian | first2=K. D. | title=Nisin, a peptide antibiotic: Cloning and sequencing of the nisA gene and posttranslational processing of its peptide product | journal=Journal of Bacteriology | volume=171 | issue=3 | pages=1597–2101 | doi=10.1128/jb.171.3.1597-1601.1989 | pmc=209786 }}</ref> In these reactions a [[ribosome|ribosomally]] synthesized 57-mer is converted to the final peptide. The unsaturated amino acids originate from [[serine]] and [[threonine]], and the enzyme-catalysed addition of [[cysteine]] residues to the didehydro amino acids result in the multiple (5) [[thioether]] bridges. |
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[[Subtilin]] and [[epidermin]] are related to nisin. All are members of a class of molecules known as [[lantibiotics]]. |
[[Subtilin]] and [[epidermin]] are related to nisin. All are members of a class of molecules known as [[lantibiotics]]. |
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==Properties== |
==Properties== |
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While most [[bacteriocins]] generally inhibit only closely related species, nisin is a rare example of a "broad-spectrum" bacteriocin effective against many [[Gram-positive]] organisms, including [[lactic acid bacteria]] (commonly associated with avoiding food spoilage),'' [[Listeria monocytogenes]]'' (a known pathogen), ''[[Staphylococcus aureus]]'', ''[[Bacillus cereus]]'', ''[[Clostridium botulinum]]'', etc.<ref name=":0">{{Cite journal|last1=And|first1=H. Chen|last2=Hoover|first2=D. G.|date=2003|title=Bacteriocins and their Food Applications|url=https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1541-4337.2003.tb00016.x|journal=Comprehensive Reviews in Food Science and Food Safety|language=en|volume=2|issue=3|pages=82–100|doi=10.1111/j.1541-4337.2003.tb00016.x|pmid=33451234 |issn=1541-4337}}</ref> It is also particularly effective against spores. [[Gram-negative]] bacteria are protected by their outer membrane but may become susceptible to nisin action after a heat shock or when this is coupled with the chelator [[EDTA]]. When used in combination with EDTA, nisin can inhibit ''[[E. coli O157:H7]]'' and ''[[Salmonella enterica]]''.<ref name=":0" /> Nisin, as a class I bacteriocin, is very stable at acidic pHs and is more heat stable at lower pHs.<ref name=":0" /> The mode of action of Nisin against pathogens such as [[Listeria monocytogenes |
While most [[bacteriocins]] generally inhibit only closely related species, nisin is a rare example of a "broad-spectrum" bacteriocin effective against many [[Gram-positive]] organisms, including [[lactic acid bacteria]] (commonly associated with avoiding food spoilage),'' [[Listeria monocytogenes]]'' (a known pathogen), ''[[Staphylococcus aureus]]'', ''[[Bacillus cereus]]'', ''[[Clostridium botulinum]]'', etc.<ref name=":0">{{Cite journal|last1=And|first1=H. Chen|last2=Hoover|first2=D. G.|date=2003|title=Bacteriocins and their Food Applications|url=https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1541-4337.2003.tb00016.x|journal=Comprehensive Reviews in Food Science and Food Safety|language=en|volume=2|issue=3|pages=82–100|doi=10.1111/j.1541-4337.2003.tb00016.x|pmid=33451234 |issn=1541-4337}}</ref> It is also particularly effective against spores. [[Gram-negative]] bacteria are protected by their outer membrane but may become susceptible to nisin action after a heat shock or when this is coupled with the chelator [[EDTA]]. When used in combination with EDTA, nisin can inhibit ''[[E. coli O157:H7]]'' and ''[[Salmonella enterica]]''.<ref name=":0" /> Nisin, as a class I bacteriocin, is very stable at acidic pHs and is more heat stable at lower pHs.<ref name=":0" /> The mode of action of Nisin against pathogens such as ''[[Listeria monocytogenes]]'' is to dissipate the membrane potential and pH gradient.<ref name=":0" /> |
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Nisin is soluble in water and effective at levels nearing the parts-per-billion range. Nisin concentration can be measured using various techniques such as [[chromatography]] or by a simple [[Agar diffusion test|agar diffusion bioassay]].<ref>{{cite journal|last1=Chandrasekar|first1=Vaishnavi|title=Modeling development of inhibition zones in an agar diffusion bioassay|journal=Food Science |
Nisin is soluble in water and effective at levels nearing the parts-per-billion range. Nisin concentration can be measured using various techniques such as [[chromatography]] or by a simple [[Agar diffusion test|agar diffusion bioassay]].<ref>{{cite journal|last1=Chandrasekar|first1=Vaishnavi|title=Modeling development of inhibition zones in an agar diffusion bioassay|journal=Food Science & Nutrition|volume=3|issue=5|pages=394–403|doi=10.1002/fsn3.232|pmid=26405525|pmc=4576963|year=2015}}</ref> |
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== Applications == |
== Applications == |
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Nisin has also been used in food packaging applications and can serve as a preservative by controlled release onto the food surface from the polymer packaging.<ref>{{Cite journal|last=Chandrasekar|first=Vaishnavi|year=2017|title=Release Kinetics of Nisin from Chitosan–Alginate Complex Films|journal=Journal of Food Science|volume=81|issue=10|pages=E2503–E2510|doi=10.1111/1750-3841.13443|pmid=27635864}}</ref> |
Nisin has also been used in food packaging applications and can serve as a preservative by controlled release onto the food surface from the polymer packaging.<ref>{{Cite journal|last=Chandrasekar|first=Vaishnavi|year=2017|title=Release Kinetics of Nisin from Chitosan–Alginate Complex Films|journal=Journal of Food Science|volume=81|issue=10|pages=E2503–E2510|doi=10.1111/1750-3841.13443|pmid=27635864}}</ref> |
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In combination with [[miconazole]] it has been studied as a possible treatment for infections of ''[[ |
In combination with [[miconazole]], it has been studied as a possible treatment for infections of ''[[Clostridioides difficile (bacteria)|Clostridioides difficile]]''.{{citation needed|date=June 2021}} |
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== Further reading == |
== Further reading == |
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* https://phys.org/news/2024-02-common-food-unexpected-effects-gut.amp |
* https://phys.org/news/2024-02-common-food-unexpected-effects-gut.amp |
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* Encyclopedia of Food Microbiology - Page 187 books.google.ae/books?{{ISBN|0123847338}} |
* Encyclopedia of Food Microbiology - Page 187 books.google.ae/books?{{ISBN|0123847338}} |
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* {{cite journal | doi = 10.1021/acschembio.3c00577 | title=Activity of Gut-Derived Nisin-like Lantibiotics against Human Gut Pathogens and Commensals| year=2024 | journal=ACS Publications | last1 = Zhang | first1 = Zhenrun J. | last2 = Wu | first2= Chunyu | last3 = Moreira | first3 = Ryan | last4 = Dorantes | first4 = Darian | last5 = Pappas | first5 = Téa | last6 = Sundararajan | first6 = Anitha | last7 = Huaiying | first7 = Lin | last8 = Pamer | first8= Eric G. | last9= van der Donk | first9= Wilfred A.| volume=19| issue=2| pages=357–369| doi-access = free | pmid=38293740| pmc = 10877564 }} |
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* This Common Food Preservative May Not Be as Harmless as We Thought : ScienceAlert [https://www.sciencealert.com/this-common-food-preservative-may-not-be-as-harmless-as-we-thought] |
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==References== |
==References== |
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Latest revision as of 07:10, 27 November 2024
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| Identifiers | |
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3D model (JSmol)
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| ChEBI | |
| ChEMBL | |
| ChemSpider | |
| ECHA InfoCard | 100.014.370 |
| E number | E234 (preservatives) |
PubChem CID
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| UNII | |
CompTox Dashboard (EPA)
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| Properties | |
| C143H230N42O37S7 | |
| Molar mass | 3354.07 g/mol |
| Appearance | powder |
| Density | 1.402 g/mL |
| Boiling point | 2,966 °C (5,371 °F; 3,239 K) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Nisin is a polycyclic antibacterial peptide produced by the bacterium Lactococcus lactis that is used as a food preservative. It has 34 amino acid residues, including the uncommon amino acids lanthionine (Lan), methyllanthionine (MeLan), didehydroalanine (Dha), and didehydroaminobutyric acid (Dhb). These unusual amino acids are introduced by posttranslational modification of the precursor peptide.[1] In these reactions a ribosomally synthesized 57-mer is converted to the final peptide. The unsaturated amino acids originate from serine and threonine, and the enzyme-catalysed addition of cysteine residues to the didehydro amino acids result in the multiple (5) thioether bridges.
Subtilin and epidermin are related to nisin. All are members of a class of molecules known as lantibiotics.
In the food industry, nisin is obtained from the culturing of L. lactis on natural substrates, such as dextrose, and it is not chemically synthesized.
It was originally isolated in the late 1930s, and produced since the 1950s as Nisaplin from naturally occurring sources by Aplin and Barrett in laboratories in Beaminster in Dorset (now owned by International Flavors & Fragrances), and approved as an additive for food use in the US in the late 1960s.[2]
Properties
[edit]While most bacteriocins generally inhibit only closely related species, nisin is a rare example of a "broad-spectrum" bacteriocin effective against many Gram-positive organisms, including lactic acid bacteria (commonly associated with avoiding food spoilage), Listeria monocytogenes (a known pathogen), Staphylococcus aureus, Bacillus cereus, Clostridium botulinum, etc.[3] It is also particularly effective against spores. Gram-negative bacteria are protected by their outer membrane but may become susceptible to nisin action after a heat shock or when this is coupled with the chelator EDTA. When used in combination with EDTA, nisin can inhibit E. coli O157:H7 and Salmonella enterica.[3] Nisin, as a class I bacteriocin, is very stable at acidic pHs and is more heat stable at lower pHs.[3] The mode of action of Nisin against pathogens such as Listeria monocytogenes is to dissipate the membrane potential and pH gradient.[3]
Nisin is soluble in water and effective at levels nearing the parts-per-billion range. Nisin concentration can be measured using various techniques such as chromatography or by a simple agar diffusion bioassay.[4]
Applications
[edit]Food production
[edit]Nisin is used in processed cheese, meats, beverages, etc. during production to extend shelf life by suppressing Gram-positive spoilage and pathogenic bacteria.[citation needed] In foods, it is common to use nisin at levels ranging from ~1-25 ppm, depending on the food type and regulatory approval. As a food additive, nisin has an E number of E234.
Other
[edit]Due to its naturally selective spectrum of activity, it is also employed as a selective agent in microbiological media to isolate gram-negative bacteria, yeast, and moulds.
Nisin has also been used in food packaging applications and can serve as a preservative by controlled release onto the food surface from the polymer packaging.[5]
In combination with miconazole, it has been studied as a possible treatment for infections of Clostridioides difficile.[citation needed]
Further reading
[edit]- Fukase, Koichi; Kitazawa, Manabu; Sano, Akihiko; Shimbo, Kuniaki; Fujita, Hiroshi; Horimoto, Shingo; Wakamiya, Tateaki; Shiba, Tetsuo (1988). "Total synthesis of peptide antibiotic nisin". Tetrahedron Letters. 29 (7): 795–798. doi:10.1016/s0040-4039(00)80212-9. (Total synthesis)
- Buchman, GW; Banerjee, S; Hansen, JN (1988). "Structure, expression, and evolution of a gene encoding the precursor of nisin, a small protein antibiotic". J Biol Chem. 263 (31): 16260–6. doi:10.1016/S0021-9258(18)37587-2. PMID 3141403. (Biosynthesis)
- http://medicalxpress.com/news/2012-10-common-food-tumor-growth.html
- https://phys.org/news/2024-02-common-food-unexpected-effects-gut.amp
- Encyclopedia of Food Microbiology - Page 187 books.google.ae/books?ISBN 0123847338
- Zhang, Zhenrun J.; Wu, Chunyu; Moreira, Ryan; Dorantes, Darian; Pappas, Téa; Sundararajan, Anitha; Huaiying, Lin; Pamer, Eric G.; van der Donk, Wilfred A. (2024). "Activity of Gut-Derived Nisin-like Lantibiotics against Human Gut Pathogens and Commensals". ACS Publications. 19 (2): 357–369. doi:10.1021/acschembio.3c00577. PMC 10877564. PMID 38293740.
- This Common Food Preservative May Not Be as Harmless as We Thought : ScienceAlert [1]
References
[edit]- ^ Kaletta, C.; Entian, K. D. (1989). "Nisin, a peptide antibiotic: Cloning and sequencing of the nisA gene and posttranslational processing of its peptide product". Journal of Bacteriology. 171 (3): 1597–2101. doi:10.1128/jb.171.3.1597-1601.1989. PMC 209786. PMID 2493449.
- ^ ageconsearch
.umn .edu /bitstream /90779 /2 /CP%2001%2005%20Nisin%20Report .pdf - ^ a b c d And, H. Chen; Hoover, D. G. (2003). "Bacteriocins and their Food Applications". Comprehensive Reviews in Food Science and Food Safety. 2 (3): 82–100. doi:10.1111/j.1541-4337.2003.tb00016.x. ISSN 1541-4337. PMID 33451234.
- ^ Chandrasekar, Vaishnavi (2015). "Modeling development of inhibition zones in an agar diffusion bioassay". Food Science & Nutrition. 3 (5): 394–403. doi:10.1002/fsn3.232. PMC 4576963. PMID 26405525.
- ^ Chandrasekar, Vaishnavi (2017). "Release Kinetics of Nisin from Chitosan–Alginate Complex Films". Journal of Food Science. 81 (10): E2503–E2510. doi:10.1111/1750-3841.13443. PMID 27635864.