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Diauxic growth

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+#REDIRECT [[Diauxic growth]]
-'''Diauxie''' is a French word meaning double growth. The word is used in English in [[cell biology]] to describe the growth phases of a [[microorganism]] in batch culture as it [[metabolism|metabolizes]] a mixture of two sugars. Rather than metabolizing the two available sugars simultaneously, microbial cells commonly consume them in a sequential pattern, resulting in two separate growth phases.  During the first phase, cells preferentially metabolize the sugar on which it can grow faster (often [[glucose]] but not always). Only after the first sugar has been exhausted do the cells switch to the second. At the time of the "diauxic shift", there is often a lag period during which the cell produces the [[enzymes]] needed to metabolize the second sugar.
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-[[Jacques Monod]] discovered diauxic growth in 1941 during his experiments with ''[[Escherichia coli]]'' and ''[[Bacillus subtilis]]''.   While growing these bacteria on various combination of sugars during his doctoral thesis research, Monod observed that often two distinct growth phases are clearly visible in batch culture.<ref>{{cite web|last = Mulligan|first = Martin|title = Induction|url=http://www.mun.ca/biochem/courses/4103/topics/induction.html|accessdate = 2007-01-01}}</ref>  Focusing on the well defined diauxic growth of ''E. coli'' on the mixture of glucose and [[lactose]], his later work led to the [[lac operon]] model of [[gene expression]].
-Diauxie occurs because organisms use multiple sets of [[genes]] to metabolize the different nutrients or sugars they encounter. If an organism allocates energy and other resources to metabolize a sugar that can only support a slower growth rate compared to the faster growth rate on a different sugar, such an organism will be at a reproductive disadvantage. Through [[evolution]], organisms have developed the ability to regulate their genetic control mechansims so as only to express those genes resulting in the fastest growth rate. For example, when grown in the presence of both glucose and maltose, ''[[Lactococcus lactis]]'' will produce enzymes to metabolize glucose first, altering its gene expression to use maltose only after the supply of glucose has been exhausted.
-In the case of the baker's or brewer's [[yeast]] ''[[Saccharomyces cerevisiae]]'' growing on glucose with plenty of aeration, the diauxic growth pattern is commonly observed in batch culture.  During the first growth phase, when there is plenty of glucose and oxygen available, the yeast cells prefer [[glucose]] [[Fermentation (biochemistry)|fermentation]] to [[aerobic respiration]] even though aerobic respiration is  the more efficient pathway to grow on glucose.   The intracellular genetic regulatory mechanisms have evolved to enforce this choice, as fermentation provides a faster growth rate for the yeast cells than the aerobic respiration of glucose.  After glucose is depleted, the fermentative product ethanol is oxidised in a noticeably slower second growth phase, if oxygen is available.
-== Mechanism ==
-In the 1940s, Monod hypothesized that a single enzyme could adapt to metabolize different sugars. It took 15 years of further work to show that this was incorrect. During his work on the [[lac operon]] of ''E. coli'', [[Joshua Lederberg]] isolated [[β-galactosidase]] and found it in greater quantities in colonies grown on lactose compared to other sugars. [[Melvin Cohn]] in Monod's lab at the Pasteur Institute then found that β-galactosides induced enzyme activity. The idea of enzyme adaptation was thus replaced with the concept of [[enzyme induction]], in which a molecule induces expression of a gene or operon, often by binding to a repressor protein and preventing it from attaching to the operator.<ref>{{cite web|last = Mulligan|first = Martin|title = Induction|url=http://www.mun.ca/biochem/courses/4103/topics/induction.html|accessdate = 2007-01-01}}</ref>
-In the case of the bacterial diauxic shift from glucose to lactose metabolism, glucose initially inhibits the ability of the enzyme [[adenylate cyclase]] to synthesize [[cyclic AMP]] (cAMP). cAMP, in turn, is required for the [[Catabolite Activator Protein|catabolite activator protein]] (CAP) to bind to DNA and activate the [[transcription (genetics)|transcription]] of the lac operon, which includes genes necessary for lactose metabolism. The presence of lactose is sensed through the activity of the [[lac repressor]], which inhibits transcription of the lac operon unless it is bound by lactose. Thus, if glucose is present, cAMP levels remain low, so CAP is unable to activate transcription of the lac operon, regardless of the presence or absence of lactose. Upon the exhaustion of the glucose supply, cAMP levels rise, allowing CAP to activate the genes necessary for the metabolism of other food sources, including lactose if it is present.<ref>{{cite web|last = Brown|first = T.A.|title = Transient Changes in Gene Activity|url=http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=genomes.section.7882|accessdate = 2007-01-01}}</ref>
-==References==
-<references/>
-[[de:Diauxie]]
-[[nl:Catabolietrepressie]]
 [[Category:Cell biology]]