High-power field: Difference between revisions
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A '''high-power field''' ('''HPF'''), when used in relation to [[microscopy]], references the [[field of view]] under the maximum magnification power of the objective being used. Often, this represents a 400-fold [[magnification]] when referenced in scientific papers. |
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==Area== |
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Area per high-power field for some microscope types: |
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*'''Olympus''' BX50, BX40 or BH2 or AO: 0.096 mm<sup>2</sup><ref name=stanford/> |
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*'''AO''' with 10x eyepiece: 0.12 mm<sup>2</sup><ref name=stanford/> |
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*'''Olympus''' with 10x eyepiece: 0.16 mm<sup>2</sup><ref name=stanford/> |
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*'''Nikon Eclipse E400''' with 10x eyepiece and 40x objective: 0.25mm<sup>2</sup><ref>{{cite web|url=https://patholines.org/Neuroendocrine_tumors_of_the_midgut|title=Neuroendocrine tumors of the midgut|author=Mikael Häggström|date=2020-01-29|website=Patholines.org}}</ref> |
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*'''Leitz Ortholux''': 0.27 mm<sup>2</sup><ref name=stanford/> |
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*'''Leitz Diaplan''': 0.31 mm<sup>2</sup><ref name=stanford/> |
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==Examples of usage== |
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The area provides a reference unit, for example in [[reference ranges for urine tests]].<ref name=southwest>[http://pathcuric1.swmed.edu/PathDemo/nrrt.htm Normal Reference Range Table] {{webarchive|url=https://web.archive.org/web/20111225185659/http://pathcuric1.swmed.edu/PathDemo/nrrt.htm |date=2011-12-25 }} from the [[University of Texas Southwestern Medical Center]]. Used in interactive case study companion to pathologic basis of disease.</ref> |
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Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based |
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on the degree of differentiation, the average number of |
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[[mitoses]] per '''high-power field''', cellularity, [[pleomorphism (cytology)|pleomorphism]], |
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and an estimate of the extent of [[necrosis]] (presumably a |
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reflection of rate of growth). Mitotic counts and necrosis |
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are the most important predictors.<ref>Robbins Basic Pathology, 9e pg 792</ref> |
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The following grading is part of [[classification of breast cancer]]: |
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{|class="wikitable" |
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|+ Mitotic count per 10 high-power fields (HPFs)<ref name=stanford>Unless otherwise specified in list/table, then reference is: {{cite web|url=http://surgpathcriteria.stanford.edu/breast/infductcabr/grading.html|title=Infiltrating Ductal Carcinoma of the Breast (Carcinoma of No Special Type)|website=[[Stanford University School of Medicine]]|accessdate=2019-10-02|archive-date=2019-09-11|archive-url=https://web.archive.org/web/20190911054536/http://surgpathcriteria.stanford.edu/breast/infductcabr/grading.html|url-status=dead}}</ref> |
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!colspan=5| Area per HPF !!rowspan=2| Score |
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|- |
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! 0.096 mm<sup>2</sup> !! 0.12 mm<sup>2</sup> !! 0.16 mm<sup>2</sup>> !! 0.27 mm<sup>2</sup> !! 0.31 mm<sup>2</sup> |
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|- |
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| 0-3 || 0-4 || 0-5 || 0-9 || 0-11 || 1 |
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|- |
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| 4-7 || 5-8 || 6-10 || 10-19 || 12-22 || 2 |
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|- |
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| >7 || >8 || >10 || >19 || >22 || 3 |
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|} |
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==References== |
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{{Reflist}} |
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{{Portal bar|Science}} |
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[[Category:Microscopy]] |
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{{optics-stub}} |
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Latest revision as of 13:52, 7 August 2024
A high-power field (HPF), when used in relation to microscopy, references the field of view under the maximum magnification power of the objective being used. Often, this represents a 400-fold magnification when referenced in scientific papers.
Area
[edit]Area per high-power field for some microscope types:
- Olympus BX50, BX40 or BH2 or AO: 0.096 mm2[1]
- AO with 10x eyepiece: 0.12 mm2[1]
- Olympus with 10x eyepiece: 0.16 mm2[1]
- Nikon Eclipse E400 with 10x eyepiece and 40x objective: 0.25mm2[2]
- Leitz Ortholux: 0.27 mm2[1]
- Leitz Diaplan: 0.31 mm2[1]
Examples of usage
[edit]The area provides a reference unit, for example in reference ranges for urine tests.[3]
Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based on the degree of differentiation, the average number of mitoses per high-power field, cellularity, pleomorphism, and an estimate of the extent of necrosis (presumably a reflection of rate of growth). Mitotic counts and necrosis are the most important predictors.[4]
The following grading is part of classification of breast cancer:
| Area per HPF | Score | ||||
|---|---|---|---|---|---|
| 0.096 mm2 | 0.12 mm2 | 0.16 mm2> | 0.27 mm2 | 0.31 mm2 | |
| 0-3 | 0-4 | 0-5 | 0-9 | 0-11 | 1 |
| 4-7 | 5-8 | 6-10 | 10-19 | 12-22 | 2 |
| >7 | >8 | >10 | >19 | >22 | 3 |
References
[edit]- ^ a b c d e f Unless otherwise specified in list/table, then reference is: "Infiltrating Ductal Carcinoma of the Breast (Carcinoma of No Special Type)". Stanford University School of Medicine. Archived from the original on 2019-09-11. Retrieved 2019-10-02.
- ^ Mikael Häggström (2020-01-29). "Neuroendocrine tumors of the midgut". Patholines.org.
- ^ Normal Reference Range Table Archived 2011-12-25 at the Wayback Machine from the University of Texas Southwestern Medical Center. Used in interactive case study companion to pathologic basis of disease.
- ^ Robbins Basic Pathology, 9e pg 792
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