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==Do Not Change-Homework==
==Do Not Change-Homework==


Science Fair Procedure
Technology has changed our lives. From man invented the flint, he has constantly tried to use tools to improve our standard for living . Since then, we have invented the wheel, the car, the bootleg DVD…

Technology has helped us in many ways. We have been able do things efficiently and to a greater extent. We
Materials:
· 1500 ml Water
· 3 Glass bowls
· 9 disposable bowls
· Cortex Magnoliae Officialis
· Sculletaria Baicalensis
· Zanthoxylum Burganeum Maxin
· Pots
· Stove
· 250 ml Graduated Cylinder
· 70% Ethanol (sephitol)
· Marker
· Coffee filter
· Funnel
· Test Tubes
· Innoculating Loop
· Pre-prepared Escherichia Coli
· 2 Test-tubes containing nutrients and suspension fluid
· Alcohol Lamp
· Matches
· Gloves
· Petri Dish
· 7 glass vials
· 50% Ampicillin Solution
· Pipette Gun
· Sterile Water
· 1 hole punch
· Ethanol Solution
· Bowl
· Tweezers
· Filter Paper
· 14 Agar plates
· Pipettes

Procedure:

I-Extraction
1.) Gathered Materials:
· 1500 ml Water
· 3 Glass bowls
· 9 disposable bowls
· Cortex Magnoliae Officialis
· Sculletaria Baicalensis
· Zanthoxylum Burganeum Maxin
· Pots
· Stove
· 250 ml Graduated Cylinder
· 70% Ethanol (sephitol)
· Marker
· Coffee filter
· Funnel
· Test Tubes

2.) Measure 50g of each herb into disposable bowls labeled 1-3

3.) Place each bowl of herbs with 500 ml of water in 3 pots by pouring the entire contents of the graduated cylinder twice.

4.) Boil on a stove at low heat.
· Start Time: 2:05
· End Time: 3:25

5.) Removed pots from stove

6.) Sterilized Funnel

7.) Opened test tube and inserted funnel

8.) Filter is folded and inserted into funnel

9.) Solution is poured and filtered into the test tubes.

10.) Refrigerate solutions.

II.) Suspension of Escherichia Coli
1.) Gathered Materials
· Innoculating Loop
· Pre-prepared Escherichia Coli
· 2 Test-tubes containing nutrients and suspension fluid
· Alcohol Lamp
· Matches
· Gloves
· 70% alcohol solution

2.) Wear gloves

3.) Sterilize hands by spraying 70% alcohol solution

4.) Sterilize inoculating loop by using an alcohol lamp

5.) Applied small amounts of E. Coli to each tube and the Petri dish with inoculating loop by scraping the bottom of the bacteria culture and stirring in the tube.

6.) Innoculated at room temperature
· Refrigerated 48 hours later to prevent overgrowth.

III.) Transfer/ Aliquote of solutions

1.) Gathered Materials
· 7 glass vials
· 3 herbal solutions
· 50% Ampicillin solution
· Pipette Gun
· Sterile Water
· Marker
· Gloves
· 70% Alcohol Solution

2.) Labeled 7 Vials
· 1-Sculletaria Baicalensis
· 2-Cortex Magnoliae Officialis
· 3-Zanthoxylum Burganeum Maxim
· 4-Ampicillin-50% solution
· 5-Ampicillin-10% solution
· 6-Ampicillin-2% solution
· 7-Sterile Water

3.) aliquote 1 ml of prepared sample 1,2,3 into vials 1,2,3. (2g of raw material per ml)

4.) apply 800 ml of sterile water into vials 5 and 6

5.) aliquote 1 ml of Ampicillin 50% solution into vial 4

6.) Put 200ml of Ampicillin 50% solution into vial 5

7.)


== смертью ==
== смертью ==

Revision as of 02:00, 23 January 2007

Welcome to Insane Ostrich Village. Cannibalism is the national religion.

***...you need to learn the wiki-formating

_____________???

Do Not Change-Homework

Science Fair Procedure

Materials: · 1500 ml Water · 3 Glass bowls · 9 disposable bowls · Cortex Magnoliae Officialis · Sculletaria Baicalensis · Zanthoxylum Burganeum Maxin · Pots · Stove · 250 ml Graduated Cylinder · 70% Ethanol (sephitol) · Marker · Coffee filter · Funnel · Test Tubes · Innoculating Loop · Pre-prepared Escherichia Coli · 2 Test-tubes containing nutrients and suspension fluid · Alcohol Lamp · Matches · Gloves · Petri Dish · 7 glass vials · 50% Ampicillin Solution · Pipette Gun · Sterile Water · 1 hole punch · Ethanol Solution · Bowl · Tweezers · Filter Paper · 14 Agar plates · Pipettes

Procedure:

I-Extraction 1.) Gathered Materials: · 1500 ml Water · 3 Glass bowls · 9 disposable bowls · Cortex Magnoliae Officialis · Sculletaria Baicalensis · Zanthoxylum Burganeum Maxin · Pots · Stove · 250 ml Graduated Cylinder · 70% Ethanol (sephitol) · Marker · Coffee filter · Funnel · Test Tubes

2.) Measure 50g of each herb into disposable bowls labeled 1-3

3.) Place each bowl of herbs with 500 ml of water in 3 pots by pouring the entire contents of the graduated cylinder twice.

4.) Boil on a stove at low heat. · Start Time: 2:05 · End Time: 3:25

5.) Removed pots from stove

6.) Sterilized Funnel

7.) Opened test tube and inserted funnel

8.) Filter is folded and inserted into funnel

9.) Solution is poured and filtered into the test tubes.

10.) Refrigerate solutions.

II.) Suspension of Escherichia Coli 1.) Gathered Materials · Innoculating Loop · Pre-prepared Escherichia Coli · 2 Test-tubes containing nutrients and suspension fluid · Alcohol Lamp · Matches · Gloves · 70% alcohol solution

2.) Wear gloves

3.) Sterilize hands by spraying 70% alcohol solution

4.) Sterilize inoculating loop by using an alcohol lamp

5.) Applied small amounts of E. Coli to each tube and the Petri dish with inoculating loop by scraping the bottom of the bacteria culture and stirring in the tube.

6.) Innoculated at room temperature · Refrigerated 48 hours later to prevent overgrowth.

III.) Transfer/ Aliquote of solutions

1.) Gathered Materials · 7 glass vials · 3 herbal solutions · 50% Ampicillin solution · Pipette Gun · Sterile Water · Marker · Gloves · 70% Alcohol Solution

2.) Labeled 7 Vials · 1-Sculletaria Baicalensis · 2-Cortex Magnoliae Officialis · 3-Zanthoxylum Burganeum Maxim · 4-Ampicillin-50% solution · 5-Ampicillin-10% solution · 6-Ampicillin-2% solution · 7-Sterile Water

3.) aliquote 1 ml of prepared sample 1,2,3 into vials 1,2,3. (2g of raw material per ml)

4.) apply 800 ml of sterile water into vials 5 and 6

5.) aliquote 1 ml of Ampicillin 50% solution into vial 4

6.) Put 200ml of Ampicillin 50% solution into vial 5

7.)

смертью

стран соединяйтесь!!!

Со смертью пожизненного президента Сапармурата Ниязова у правительства Туркменистана появляется шанс положить конец многолетним репрессиям и открыть новую главу для прав человека в этой богатой газом центральноазиатской стране. За 21 год своего правления Ниязов превратил Туркменистан в одно из самых закрытых и репрессивных государств мира. Туркменбаши не терпел никакой оппозиции и гражданского общества, отправляя за решетку или в эмиграцию оппозиционеров, правозащитников, журналистов и членов их семей. Постоянные политически чистки и гротескный культ личности напоминали сталинизм. Социально-экономическая политика Ниязова отбрасывала страну назад. Хьюман Райтс Вотч призывает правительство Туркменистана немедленно освободить всех политзаключенных, отменить ограничения на свободу передвижения в стране, а также на въезд и выезд, и провести свободные и справедливые президентские выбор В условиях, когда голос Соединенных Штатов утратил вес из-за использования администрацией Буша-младшего пыток и содержания под стражей без суда, образовавшуюся брешь в глобальном правозащитном лидерстве должен заполнить Евросоюз, говорится в публикуемом сегодня Всемирном докладе Хьюман Райтс Вотч

75.82.205.58 12:36, 13 January 2007 (UTC)[reply]

Hey B**j*m*n Z***

you know on the sequoia page i think you should change the vice principle cause i dunno what the new persons name is. also click on her link. isn't this how you feel too?


Josephseagullstalin 01:53, 17 January 2007 (UTC)[reply]