MRC-5: Difference between revisions
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{{short description|Cell line}} |
{{short description|Cell line}} |
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[[File:MERS Coronavirus Particles (14702511068).jpg|thumb|MRC-5 cell]] |
[[File:MERS Coronavirus Particles (14702511068).jpg|thumb|MRC-5 cell]] |
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'''MRC-5''' ([[Medical Research Council (United Kingdom)|Medical Research Council]] cell strain 5) is a diploid [[cell culture]] line composed of [[fibroblast]]s, originally developed from the [[lung]] tissue of a 14-week-old [[abortion|aborted]] Caucasian male [[fetus]].<ref name=atcc>{{cite web|title=MRC-5 (ATCC® CCL-171™)|url=http://www.atcc.org/Products/All/CCL-171.aspx|accessdate=2016-05-19}}</ref><ref name=Coriell>{{cite web|title=AG05965-D Fibroblast from Skin, Lung|url=https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AG05965-D|accessdate=2016-05-19}}</ref> The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 cells themselves are known to reach [[Cellular senescence|senescence]] in around 45 population doublings.<ref name=Coriell/><ref name=Jacobs1970>{{cite journal|last=Jacobs|first=J. P.|last2=Jones|first2=C. M.|last3=Baille|first3=J. P.|title=Characteristics of a Human Diploid Cell Designated MRC-5|journal=Nature|date=1970|volume=227|issue=5254|pages=168–70|doi=10.1038/227168a0|pmid=4316953}}</ref><ref name=Jacobs1976>{{cite journal|last=Jacobs|first=J. P.|title=The Status of Human Diploid Cell Strain MRC-5 as an Approved Substrate for the Production of Viral Vaccines|journal=Journal of Biological Standardization|date=1976|volume=4|issue=2|pages=97–99|doi=10.1016/0092-1157(76)90018-4|pmid=932048}}</ref> |
'''MRC-5''' ([[Medical Research Council (United Kingdom)|Medical Research Council]] cell strain 5) is a diploid [[cell culture]] line composed of [[fibroblast]]s, originally developed from the [[lung]] tissue of a 14-week-old [[abortion|aborted]] Caucasian male [[fetus]].<ref name=atcc>{{cite web|title=MRC-5 (ATCC® CCL-171™)|url=http://www.atcc.org/Products/All/CCL-171.aspx|accessdate=2016-05-19}}</ref><ref name=Coriell>{{cite web|title=AG05965-D Fibroblast from Skin, hou hi hou houden hsuegbsksibr. Lung|url=https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=AG05965-D|accessdate=2016-05-19}}</ref> The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 cells themselves are known to reach [[Cellular senescence|senescence]] in around 45 population doublings.<ref name=Coriell/><ref name=Jacobs1970>{{cite journal|last=Jacobs|first=J. P.|last2=Jones|first2=C. M.|last3=Baille|first3=J. P.|title=Characteristics of a Human Diploid Cell Designated MRC-5|journal=Nature|date=1970|volume=227|issue=5254|pages=168–70|doi=10.1038/227168a0|pmid=4316953}}</ref><ref name=Jacobs1976>{{cite journal|last=Jacobs|first=J. P.|title=The Status of Human Diploid Cell Strain MRC-5 as an Approved Substrate for the Production of Viral Vaccines|journal=Journal of Biological Standardization|date=1976|volume=4|issue=2|pages=97–99|doi=10.1016/0092-1157(76)90018-4|pmid=932048}}</ref> |
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==Applications== |
==Applications== |
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Revision as of 19:59, 11 November 2021
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MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted Caucasian male fetus.[1][2] The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 cells themselves are known to reach senescence in around 45 population doublings.[2][3][4]
Applications
MRC-5 cells are currently used to produce several vaccines including for hepatitis A, varicella and polio.[5]
Culture and society
During the COVID-19 pandemic, anti-vaccination and anti-abortion activists believed that MRC-5 was an ingredient of the Oxford–AstraZeneca COVID-19 vaccine, citing a study from the University of Bristol. David Matthews, a co-author for this study, clarified that MRC-5 was solely used for testing purposes to determine "how the Oxford vaccine behaves when it is inside a genetically normal human cell."[6] The manufacturing of the vaccine used the HEK 293 fetal cell line, the kidney cells of an aborted female fetus, though the cells are filtered out of the final product.[6]
See also
References
- ^ "MRC-5 (ATCC® CCL-171™)". Retrieved 2016-05-19.
- ^ a b "AG05965-D Fibroblast from Skin, hou hi hou houden hsuegbsksibr. Lung". Retrieved 2016-05-19.
- ^ Jacobs, J. P.; Jones, C. M.; Baille, J. P. (1970). "Characteristics of a Human Diploid Cell Designated MRC-5". Nature. 227 (5254): 168–70. doi:10.1038/227168a0. PMID 4316953.
- ^ Jacobs, J. P. (1976). "The Status of Human Diploid Cell Strain MRC-5 as an Approved Substrate for the Production of Viral Vaccines". Journal of Biological Standardization. 4 (2): 97–99. doi:10.1016/0092-1157(76)90018-4. PMID 932048.
- ^ "Fact-checking Congress's fetal tissue report".
- ^ a b Kim, Noah Y. (November 18, 2020). "The Oxford-AstraZeneca vaccine does not contain aborted fetal tissue". Politifact. Retrieved October 8, 2021.
External links
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