Jump to content

Separation anxiety in dogs: Difference between revisions

From Wikipedia, the free encyclopedia
Browse history interactively
← Previous editNext edit →
Content deleted Content added
VisualWikitext
Pixels3587 (talk | contribs)
117 edits
Additional information added to "Submissive and Excitement Urination" under "Behavioural related-problems"
Pixels3587 (talk | contribs)
117 edits
Additional information added to "Submissive and Excitement Urination" under "Behavioural related-problems"
Line 58: Line 58:
***
***
* Submissive and Excitement Urination<ref name=":13">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>
* Submissive and Excitement Urination<ref name=":13">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>
**Submissive and excitement-related urination usually presents in puppy's and the behaviour reduces with age. Excitement-related urination occurs during greetings or playtime, however urination can occur due to submissive behaviour which can be related to fear and nervousness<ref name=":16">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>.
**Submissive and excitement-related urination usually presents in puppy's and the behaviour reduces with age. Excitement-related urination occurs during greetings or playtime, however urination can occur due to submissive behaviour which can be related to fear and nervousness<ref name=":16">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>. In these cases, the dog may change its posture, approaching or fleeing individuals with its head and tail pointed downwards, crouching, and/or rolling over and exposing its belly; presenting submissiveness<ref name=":17">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>.
* Urine and Fecal Marking<ref name=":14">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>
* Urine and Fecal Marking<ref name=":14">{{Cite journal|last=Lindell|first=Ellen|date=2017|title=Canine House Soiling|url=https://files.brief.vet/migration/article/35531/ab_canine-house-soiling-35531-article.pdf|journal=Clinicians Brief|pages=111-114}}</ref>
* Boredom
* Boredom

Revision as of 05:50, 13 November 2021

Separation anxiety in dogs describes a condition in which a dog exhibits distress and behavior problems when separated from its handler. Separation anxiety typically manifests within minutes of departure of the handler.[1] It is not fully understood why some dogs suffer from separation anxiety and others do not.[2] The behavior may be secondary to an underlying medical condition.[3] With chronic stress, impairments to physiological health can manifest. Increased stress in the dog alters hormone levels, thus decreasing natural immunity to various health problems.[4] A visit to the veterinarian is always recommended if a dog's behavior changes suddenly.

Signs and symptoms

Dogs suffering from separation anxiety typically exhibit these behaviors:

  • Following handler excessively
  • Pacing
  • Excessive salivating
  • Excessive shaking (usually seen in smaller breeds like the Chihuahua and Yorkshire Terrier)
  • Vomiting
  • Destructive chewing
  • Barking, howling, whining
  • Urination, defecation in the house
  • Coprophagia[5]
  • Self harm
  • Digging and scratching at doors or windows in an attempt to reunite with the handler[6]
  • Escaping
  • Anorexia[7]
  • Overactivity (excessive greeting, restlessness)[7]

Cause

The cause of dog separation anxiety is unknown, but may be triggered by:

  • a traumatic event[8]
  • a change in routine[6]
  • major life change (e.g., new home, new baby, death of a family member, abandonment to a shelter[5])
  • extreme attachment or dependency on the owner[9]

Diagnosis

It can be difficult to differentiate separation anxiety from other disorders in dogs since there are no specific diagnostic tests available. There is no known genetic component to this disorder; it can occur at any point in the dog's life but has been shown to coincide with times of stress and major life changes.  

Research presented among strays indicated diagnoses of separation anxiety to be 56 percent higher, and in mixed breeds, 67 percent higher[10]. Therefore, the dogs' previous and current living conditions have an impact on the diagnosis, as many of these animals developed separation anxiety due to traumatic experiences.   

Diagnoses are made by examining dogs' behaviour before and during departure, when the owner is absent, and when the owner returns home. It is encouraged by pet professionals to record behavioural responses after departure to increase the probability of an accurate diagnosis.  By observing the dogs behaviour, professionals can exclude external factors that could influence the behaviour that would not have been identifiable without the recording[11]. Repetitive behaviours and autonomic responses such as tachycardia, tachypnea, and trembling may also be identified on a recording in the owners absence[11]. As some of the behaviours presented by the canine could be related to other medical or behavioural problems, behavioural response characteristics must be examined to provide an accurate diagnosis.

Urination and defecation are prominent behavioural response within canines experiencing separation anxiety. Elimination must only occur within one to thirty minutes after companions departure or when the canine is prevented from being near it's companion[12]. Destructive behaviour to its environment or itself can also be present immediately after departure of the owner. Behavioural responses such as chewing, digging, and scratching could apply to any personal objects or areas in the environment, but is usually directed towards possible exits (i.e., doors and windows)[13]. Vocal responses such as whining, howling, and barking could precede departure and/or begin following departure[13]. This may persist for a longer period in comparison to other behavioural responses presented preceding departure of the owner. In severe cases, excessive vocalization can lead to damage to the dog's voice box, losing the ability to bark or howl for a period. Behaviours need not persist until return of the owner, apart from vocalization, but may be present at arrival. As well, the above behaviours must be correlated with signs of distress and restlessness within the period of time leading to or immediately after the behavioural response[14].

Common Misdiagnoses

As behaviours presented through separation anxiety can be linked to many other behavioural and medical disorders, careful diagnostic criteria must be followed.

As a preventative measure, and to exclude other possible explanations for the behavioural responses, diagnostic testing is performed. A physical examination is performed, and tests relating to the animals age and medical history may be required. In relation to the elimination behaviour, a urinalysis is suggested and a stool sample may be required if it presents as abnormal or the dog is expressing other digestive issues[15].

Medical Problems

  • Urinary Incontinence related to Medical Problems
    • A large number of medical problems can influence canine house soiling, including urinary tract infection, weak sphincter caused by old age, hormone-related problems, bladder stones, diabetes, abnormalities of the genitalia, and neurological problems[16]. It is most common among large breed, spayed female dogs, but it can occur in intact females, male dogs, and cats[17]. Urethral sphincter incompetence is the primary cause of urinary incontinence[18]. It is important to seek veterinary aid if urinary incontinence presents frequently.
  • Medication
    • Medication can affect canine behaviour in relation to elimination, destruction, and vocalization. If negative behavioural patterns persist in relation to these symptoms, it is important to seek veterinary advice. It may be necessary to change or adjust the canine's prescription if it is inducing negative behavioural responses.
  • Incomplete Housetraining[19]
    • In the case of house training issues, there is no pattern or correlation within the behaviours, as elimination, destruction, and vocalization frequencies do not change[20]. However, it is more prominent in younger dogs. Behavioural responses related to incomplete housetraining do not exhibit distress or restlessness and the canine is usually not aware that they are participating in a wrongful behaviour.
  • Submissive and Excitement Urination[21]
    • Submissive and excitement-related urination usually presents in puppy's and the behaviour reduces with age. Excitement-related urination occurs during greetings or playtime, however urination can occur due to submissive behaviour which can be related to fear and nervousness[22]. In these cases, the dog may change its posture, approaching or fleeing individuals with its head and tail pointed downwards, crouching, and/or rolling over and exposing its belly; presenting submissiveness[23].
  • Urine and Fecal Marking[24]
  • Boredom
  • Abuse

Treatment

Dogs suffering from separation anxiety are often "owner addicts." Setting boundaries can boost a dog's confidence when away from the owner.[25]

Various techniques can help dogs cope with separation anxiety:

  • Leaving and returning home quietly[26]
  • Providing plenty of exercise, play, and fun[27][28]
  • Practicing the routine of leaving the house to desensitize the dog
  • Feeding the dog before departure
  • Leaving the radio/TV on
  • Medicating the dog[29]

Drug therapy

Dogs can be treated with psychotropic drugs used to treat similar psychological disorders displayed in humans in order to mitigate the behavior related to these disorders, such as anti-depressants or anti-anxiety drugs. These connections between human and animal psychopharmacology can help to explain how similar neurobiology can be among different species.[30] Selective Serotonin Reuptake Inhibitors, or SSRIs, or tricyclic anti-depressants are used to treat anxious and depressive behavior in animals. One study tracked the effectiveness of clomipramine, a tricyclic anti-depressant, in reducing compulsive behaviors through administration of a tricyclic anti-depressant in dogs. Behaviors displayed by these dogs include but are not limited to tail-chasing, shadow-chasing, circling and chewing. The study found that after one month of daily administration of the tricyclic anti-depressant clomipramine, these compulsive behaviors decreased or disappeared in 16 out of 24 dogs. Slight to moderate behavior mitigation was shown in 5 dogs. These results suggest that clomipramine can be beneficial to canines displaying anxiety behaviors.[31]

Anxiety disorders can also be treated with dog appeasing pheromones similar to those given off by their mothers.[32] The pheromone-containing products are sold as collars and sprays under the brand name Adaptil.[32]

Anti-depressant treatment

Fluoxetine, an SSRI used by humans under the brand name Prozac, is prescribed to dogs under the brand name Reconcile. A study found that dogs who were being simultaneously treated with Reconcile while undergoing a type of behavior therapy known as behavioral modulation were more successful at mitigating behaviors related to separation anxiety when compared to the control group of dogs receiving only a placebo with behavior modulation treatment. After 8 weeks of treatment, 72% of the dogs given fluoxetine displayed fewer adverse behaviors (e.g., excessive salivation, inappropriate urination/defecation) while only 50% of the control group mitigated these behaviors.[33]

In another study conducted in 2015, dogs expressing symptoms of separation anxiety were given fluoxetine tablets and a standard behavior modification plan for two months.[34] Owner interviews, spatial cognitive bias tests, questionnaires and relations between cognitive bias and drug treatment were all taken into consideration. Results showed that the clinical treatment of fluoxetine seemed to produce a shift in cognitive bias in the canine subjects, emphasizing that pharmacological therapy not only can positively affect behavior, but also an animal's psychological state.

The most common adverse effects were decreased appetite, experienced by 23% of the dogs in the study, and lethargy, experienced by 39% of the dogs in the study. Some canines actually experienced worsening anxiety and aggressive behavior.[35]

In a study using the anti-depressant clomipramine, nine dogs underwent withdrawal after discontinuing treatment. Five of those dogs were successful in overcoming the withdrawal, while four dogs relapsed. Although the study's sample sizes were relatively small, it illuminated one of the many variables regarding psychoactive drug withdrawal.[36]

Benzodiazepine treatment

Benzodiazepines, such as alprazolam, are anxiolytic medications. Benzodiazepines are beneficial in the treatment of stimuli-evoking anxiety, or phobias. One study on storm phobias found that 30 out of the 32 canines involved in the study had reductions in anxious behaviors after being treated with alprazolam. However, this study found that the best way to benefit from benzodiazepine treatment is when it is being used in conjunction with behavior modulation treatment and an anti-depressant.[37]

The study found that canines can develop dependence to these types of medications and experience a withdrawal process similar to one experienced by humans. For example, their seizure threshold is lowered and anxiety relapse can occur after stopping benzodiazepine treatment.[38] Similarly to treatment of human anxiety disorders, benzodiazepines are a last resort treatment, due to their addiction potential.[39] Selective Serotonin Reuptake Inhibitors, or SSRIs, or tricyclic anti-depressants are used to treat anxious and depressive behavior in animals. One study tracked the effectiveness of clomipramine, a tricyclic anti-depressant, in reducing compulsive behaviors through administration of a tricyclic anti-depressant in dogs. Behaviors displayed by these dogs include but are not limited to tail-chasing, shadow-chasing, circling and chewing. The study found that after one month of daily administration of the tricyclic anti-depressant clomipramine, these compulsive behaviors decreased or disappeared in 16 out of 24 dogs. Slight to moderate behavior mitigation was shown in 5 dogs. These results suggest that clomipramine can be beneficial to canines displaying anxiety behaviors.[31]

Imepitoin, also known by its brand name Pexion, is a recently developed drug for treating primarily canine epilepsy. Imepitoin is a low-affinity agonist at the benzodiazepine site of the GABAA receptor, meaning it is able to loosely attach itself to the GABA receptor and mimic GABA. Gamma-aminobutyric acid (GABA) is a neurotransmitter that is used to counteract glutamate, the excitatory neurotransmitter responsible for eliciting anxious behaviors if levels are excessive, causing long-term anxiety disorders.[40] Because imepitoin is known to have anti-convulsant effects on laboratory rats and is already an anti-epilepsy drug treatment, researchers are curious as to whether or not it could decrease a canine's levels of separation anxiety, as one symptom of separation anxiety in dogs is excessive shaking, primarily in smaller breeds.

One study conducted in 2016 did not test its effects on separation anxiety specifically, but rather investigated its abilities to reduce fear and anxiety-related behaviors. The study was conducted via an online survey completed by the canine participants' owners, including data on the number of how monthly seizures the dogs experienced.[41] Results showed a significant reduction of average seizures per month, but no significant differences in behavior regarding the five anxiety-related measures examined (dog-directed fear, stranger-directed fear, non-social fear, pain sensitivity and separation-related behavior), concluding that imepitoin did not definitively affect anxiety-related behavior in dogs. However, it was noted by researchers conducting the study that the participants' anxiety levels could not have been high enough in the beginning of the study for the dogs to show a significant reduction in anxiety-related behavior.

Another research study in June 2017 tested imepitoin's abilities to reduce anxiety-related behaviors in canines,[42] but unlike the previous study, researchers evaluated the dogs personally for canine temperament using a Positive and Negative Activation Scale (PANAS), rather than having the participants' owners evaluate the dogs through an online survey. Average weekly reaction (AWR) scores in response to anxiety-inducing stimuli and owners' diary entries were also taken into account. Results displayed significantly lower AWR scores for anxiety alongside a reduction in negative activation on the PANAS, concluding that imepitoin is a drug-therapy option to positively reduce canines' anxiety-related behaviors. However, imepitoin is not being prescribed to treat separation anxiety in canines.

Nelumbinis Semen treatment

One cause of separation anxiety in canines is chronic stress. A study in 2012 tested Nelumbinis Semen, the seeds of the herb Nelumbo nucifera, and its anti-depressant effects on animals experiencing stress.[43] This study did not test directly on canines, but rather rats, and aimed to apply the principles found by the study to other animals like dogs. The study, however, did test oral toxicity specifically on canines. After testing different dosage amounts of the Nelumbinis Semen, scientists determined that 400 mg per the animal's weight in kilograms was the ideal amount to lower immobility when the animal was faced with a stressful situation. In addition, Nelumbinis Semen was not found to be toxic when administered to dogs. The study supports the possibility that alternative and "natural" ingredients may be used as a substitute for drug-based therapy.

Other scientific findings

A study conducted in 2016 used primary metabolite profiling through a combination of liquid chromatography and mass spectrometry to identify possible biomarkers for anxiety-related behavior.[44] Primary metabolites are directly involved in more "natural" processes, such as reproduction and development,[45] so abnormal differences could result in differences of mental development. Results identified changes in thirteen metabolites between dogs who had separation anxiety and those who did not; these changes included differences in hypoxanthine, indoxysulfate and phospholipids, all which control oxidative stress, tryptophan levels, and lipid metabolisms. Researchers concluded that biomarkers like primary metabolites play a prominent role in canine anxiety.

References

  1. ^ "Guide to Separation Anxiety in Dogs". PawLeaks (2020).
  2. ^ of the US, Humane Society. "Separation Anxiety". Retrieved 9 August 2012.
  3. ^ MD, Pet. "Separation Anxiety in Dogs". Retrieved 9 August 2012.
  4. ^ Dreschel, Nancy (2010). "The effects of fear and anxiety on health and lifespan in pet dogs". Applied Animal Behaviour Science. 125 (3): 157–162. doi:10.1016/j.applanim.2010.04.003.
  5. ^ a b ASPCA. "Common Dog Behavior Issues". ASPCA. Retrieved November 30, 2016.
  6. ^ a b "Separation Anxiety : The Humane Society of the United States". Retrieved 2012-03-08.
  7. ^ a b Ogata, Niwako (2016-11-01). "Separation anxiety in dogs: What progress has been made in our understanding of the most common behavioral problems in dogs?". Journal of Veterinary Behavior. 16: 28–35. doi:10.1016/j.jveb.2016.02.005. ISSN 1558-7878.
  8. ^ PAWS. "Separation Anxiety". PAWS. Retrieved 2020-09-30.
  9. ^ Takeuchi, Yukari (2000). "Evaluation of treatments for separation anxiety in dogs". Journal of the American Veterinary Medical Association. 217 (3): 342–345. doi:10.2460/javma.2000.217.342.
  10. ^ McCrave, Elizabeth A. (1991). "Diagnostic Criteria for Separation Anxiety in the Dog". Veterinary Clinics of North America: Small Animal Practice. 21 (2): 247–255. doi:10.1016/S0195-5616(91)50030-9.
  11. ^ a b Sherman, Barbara L. (2008). "Understanding Behavior: Separation Anxiety in Dogs". Behaviour Compendium. 30: 27–42.
  12. ^ McCrave, Elizabeth A. (1991). "Diagnostic Criteria for Separation Anxiety in the Dog". Veterinary Clinics of North America: Small Animal Practice. 21 (2): 247–255. doi:10.1016/S0195-5616(91)50030-9.
  13. ^ a b McCrave, Elizabeth A. (1991). "Diagnostic Criteria for Separation Anxiety in the Dog". Veterinary Clinics of North America: Small Animal Practice. 21 (2): 247–255. doi:10.1016/S0195-5616(91)50030-9.
  14. ^ McCrave, Elizabeth A. (1991). "Diagnostic Criteria for Separation Anxiety in the Dog". Veterinary Clinics of North America: Small Animal Practice. 21 (2): 247–255. doi:10.1016/S0195-5616(91)50030-9.
  15. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  16. ^ Brooks, Wendy (2021). "Urinary Incontinence in Dogs and Cats". Veterinary Partner.
  17. ^ Dowling, Patricia M. (2015). "Urinary Incontinence". Merck Veterinary Manual.
  18. ^ Dowling, Patricia M. (2015). "Urinary Incontinence". Merck Veterinary Manual.
  19. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  20. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  21. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  22. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  23. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  24. ^ Lindell, Ellen (2017). "Canine House Soiling" (PDF). Clinicians Brief: 111–114.
  25. ^ Kilcommons, Brian (2007-04-28). "How to Cure Your Dog's Separation Anxiety". Retrieved 9 August 2012.
  26. ^ "Separation Anxiety In Dogs and How to Deal with It - Coping with Destructive and Obsessive Compulsive Behaviors". Retrieved 2012-03-09.
  27. ^ "Reduce Your Pet's Anxiety When They're Home Alone". www.petsafe.net. Retrieved 2021-10-28.
  28. ^ "Dog Training - Dog Separation Anxiety". 2015-12-29. Retrieved 2016-01-11.
  29. ^ "Separation Anxiety". ASPCA. Retrieved 2020-10-01.
  30. ^ Hamby, Tori Medication now used to treat pet’s behavioral disorders September 30, 2012 http://www.huntersvilleherald.com/news/2012/09/30/medication-now-used-to-treat-pets-behavioral-disorders/
  31. ^ a b Seksel, K; Lindeman, MJ (2001). "Use of clomipramine in treatment of obsessive-compulsive disorder, separation anxiety and noise phobia in dogs: A preliminary, clinical study". Australian Veterinary Journal. 79 (4): 252–6. doi:10.1111/j.1751-0813.2001.tb11976.x. PMID 11349411.
  32. ^ a b Bowman, Alisa (September 2015). "Soothing Separation Anxiety". Prevention. 67 (9): 161–163.
  33. ^ Simpson, BS; Landsberg, GM; Reisner, IR; Ciribassi, JJ; Horwitz, D; Houpt, KA; Kroll, TL; Luescher, A; et al. (2007). "Effects of reconcile (fluoxetine) chewable tablets plus behavior management for canine separation anxiety". Veterinary Therapeutics. 8 (1): 18–31. PMID 17447222.
  34. ^ Karagiannis, Christos I.; Burman, Oliver HP; Mills, Daniel S. (2015-12-01). "Dogs with separation-related problems show a "less pessimistic" cognitive bias during treatment with fluoxetine (Reconcile™) and a behaviour modification plan". BMC Veterinary Research. 11 (1): 80. doi:10.1186/s12917-015-0373-1. ISSN 1746-6148. PMC 4393593. PMID 25889323.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  35. ^ Irimajiri, M; Luescher, AU; Douglass, G; Robertson-Plouch, C; Zimmermann, A; Hozak, R (2009). "Randomized, controlled clinical trial of the efficacy of fluoxetine for treatment of compulsive disorders in dogs". Journal of the American Veterinary Medical Association. 235 (6): 705–9. doi:10.2460/javma.235.6.705. PMID 19751167.
  36. ^ Crowell-Davis, Sharon L.; Seibert, Lynne M.; Sung, Wailani; Parthasarathy, Valli; Curtis, Terry M. (2003). "Use of clomipramine, alprazolam, and behavior modification for treatment of storm phobia in dogs". Journal of the American Veterinary Medical Association. 222 (6): 744–8. doi:10.2460/javma.2003.222.744. PMID 12675296.
  37. ^ Crowell-Davis, Sharon L.; Seibert, Lynne M.; Sung, Wailani; Parthasarathy, Valli; Curtis, Terry M. (2003). "Use of clomipramine, alprazolam, and behavior modification for treatment of storm phobia in dogs". Journal of the American Veterinary Medical Association. 222 (6): 744–8. doi:10.2460/javma.2003.222.744. PMID 12675296.
  38. ^ Frey, Hans-Hasso; Philippin, Hans-Peter; Scheuler, Wolfgang (1984). "Development of tolerance to the anticonvulsant effect of diazepam in dogs". European Journal of Pharmacology. 104 (1–2): 27–38. doi:10.1016/0014-2999(84)90365-0. PMID 6437848.
  39. ^ Hamby, Tori Medication now used to treat pet’s behavioral disorders September 30, 2012 http://www.huntersvilleherald.com/news/2012/09/30/medication-now-used-to-treat-pets-behavioral-disorders/
  40. ^ Lydiard, R. Bruce (2003). "The role of GABA in anxiety disorders". The Journal of Clinical Psychiatry. 64 Suppl 3: 21–27. ISSN 0160-6689. PMID 12662130.
  41. ^ Packer, Rowena M. A.; De Risio, Luisa; Volk, Holger A. (2017-04-07). "Investigating the potential of the anti-epileptic drug imepitoin as a treatment for co-morbid anxiety in dogs with idiopathic epilepsy". BMC Veterinary Research. 13 (1): 90. doi:10.1186/s12917-017-1000-0. ISSN 1746-6148. PMC 5383962. PMID 28388948.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  42. ^ McPeake, Kevin J.; Mills, Daniel S. (2017-06-13). "The use of imepitoin (Pexion™) on fear and anxiety related problems in dogs - a case series". BMC Veterinary Research. 13 (1): 173. doi:10.1186/s12917-017-1098-0. ISSN 1746-6148. PMC 5470190. PMID 28610629.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  43. ^ Chung, Hwan-Suck; Lee, Hye Jeong; Shim, Insop; Bae, Hyunsu (2012-05-28). "Assessment of anti-depressant effect of nelumbinis semen on rats under chronic mild stress and its subchronic oral toxicity in rats and beagle dogs". BMC Complementary and Alternative Medicine. 12: 68. doi:10.1186/1472-6882-12-68. ISSN 1472-6882. PMC 3407502. PMID 22640371.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  44. ^ Puurunen, Jenni; Tiira, Katriina; Lehtonen, Marko; Hanhineva, Kati; Lohi, Hannes (2016-02-12). "Non-targeted metabolite profiling reveals changes in oxidative stress, tryptophan and lipid metabolisms in fearful dogs". Behavioral and Brain Functions. 12 (1): 7. doi:10.1186/s12993-016-0091-2. ISSN 1744-9081. PMC 4751666. PMID 26867941.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  45. ^ "Plant Physiology|Digital Textbook Library". www.tankonyvtar.hu. Retrieved 2017-08-08.
Retrieved from "https://en.wikipedia.org/enwiki/w/index.php?title=Separation_anxiety_in_dogs&oldid=1054996427"