Buffered charcoal yeast extract agar: Difference between revisions
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'''Buffered charcoal yeast extract (BCYE)''' [[agar]] is a [[Growth medium#Selective media|selective growth medium]] used to [[microbiological culture|culture]] or grow certain types of [[bacteria]], particularly the [[Gram-negative]] species ''[[Legionella pneumophila]]''.<ref>{{cite web|url=https://www.cdc.gov/legionella/files/LegionellaProcedures-508.pdf |title=Archived copy |accessdate=2012-05-11 |url-status=dead |archiveurl=https://web.archive.org/web/20120612040950/http://www.cdc.gov/legionella/files/LegionellaProcedures-508.pdf |archivedate=2012-06-12 }}</ref> It has also been used for the laboratory diagnosis of [[Acanthamoeba keratitis|''Acanthamoeba'' keratitis]],<ref name="pmid9780107">{{cite journal |author=Penland RL, Wilhelmus KR |title=Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar |journal=Am. J. Ophthalmol. |volume=126 |issue=4 |pages=590–2 |date=October 1998 |pmid=9780107 |doi= 10.1016/S0002-9394(98)00125-1}}</ref> ''[[Francisella]]'' and ''[[Nocardia]]''. |
'''Buffered charcoal yeast extract (BCYE)''' [[agar]] is a [[Growth medium#Selective media|selective growth medium]] used to [[microbiological culture|culture]] or grow certain types of [[bacteria]], particularly the [[Gram-negative]] species ''[[Legionella pneumophila]]''.<ref>{{cite web|url=https://www.cdc.gov/legionella/files/LegionellaProcedures-508.pdf |title=Archived copy |accessdate=2012-05-11 |url-status=dead |archiveurl=https://web.archive.org/web/20120612040950/http://www.cdc.gov/legionella/files/LegionellaProcedures-508.pdf |archivedate=2012-06-12 }}</ref> It has also been used for the laboratory diagnosis of [[Acanthamoeba keratitis|''Acanthamoeba'' keratitis]],<ref name="pmid9780107">{{cite journal |author=Penland RL, Wilhelmus KR |title=Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar |journal=Am. J. Ophthalmol. |volume=126 |issue=4 |pages=590–2 |date=October 1998 |pmid=9780107 |doi= 10.1016/S0002-9394(98)00125-1}}</ref> ''[[Francisella]]'' and ''[[Nocardia]]''. |
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The growth medium is prepared from the following components: |
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|+Components of a BCYE agar medium<ref>{{cite web |last1=Aryal |first1=Sagar |title=BCYE Agar- Composition, Principle, Preparation, Results, Uses |url=https://microbenotes.com/buffered-charcoal-yeast-extract-bcye/ |website=Microbe Notes |date=4 January 2022}}</ref> |
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! Ingredient !! Amount (gram/liter) !! Purpose |
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|Agar||12.0||Solidifying |
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|[[Yeast extract]]||10.0||Vitamins, nitrogen, and carbon |
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|[[ACES (buffer)|ACES buffer]]||10.0||Buffer |
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|[[Activated charcoal]]||2.0||Decomposes hydrogen peroxide, a toxic metabolite of ''Legionella'' |
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|[[Potassium hydroxide]]||2.8||To neutralize alpha-ketoglutarate |
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|[[Alpha-ketoglutarate]]||1.0 |
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|rowspan=3|Selectively stimulate growth |
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|[[L-cysteine]]||0.4 |
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|[[Ferric pyrophosphate]]||0.2 |
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This culture is to be differentiated from regular ''yeast extract agar'', which is not selective. |
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Its selectivity stems from the inclusion of [[activated charcoal]]. Without it, the medium itself is able to support the growth of a variety of organisms. |
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==References== |
==References== |
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Revision as of 05:15, 19 January 2022
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila.[1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis,[2] Francisella and Nocardia.
| Ingredient | Amount (gram/liter) | Purpose |
|---|---|---|
| Agar | 12.0 | Solidifying |
| Yeast extract | 10.0 | Vitamins, nitrogen, and carbon |
| ACES buffer | 10.0 | Buffer |
| Activated charcoal | 2.0 | Decomposes hydrogen peroxide, a toxic metabolite of Legionella |
| Potassium hydroxide | 2.8 | To neutralize alpha-ketoglutarate |
| Alpha-ketoglutarate | 1.0 | Selectively stimulate growth |
| L-cysteine | 0.4 | |
| Ferric pyrophosphate | 0.2 |
This culture is to be differentiated from regular yeast extract agar, which is not selective.
References
- ^ "Archived copy" (PDF). Archived from the original (PDF) on 2012-06-12. Retrieved 2012-05-11.
{{cite web}}: CS1 maint: archived copy as title (link) - ^ Penland RL, Wilhelmus KR (October 1998). "Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar". Am. J. Ophthalmol. 126 (4): 590–2. doi:10.1016/S0002-9394(98)00125-1. PMID 9780107.
- ^ Aryal, Sagar (4 January 2022). "BCYE Agar- Composition, Principle, Preparation, Results, Uses". Microbe Notes.
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