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Acme is Ancient Greek (ακμή; English transliteration: akmē) for the peak, zenith or prime. It may refer to:

Acme Corporation, a fictional company originating in Looney Tunes cartoons, later a generic fictional company name
ACME Detective Agency, a fictional detective agency in the Carmen Sandiego computer games and television shows
Acme (album), an album by the Jon Spencer Blues Explosion
Acme Novelty Library, a comics serial written and drawn by Chris Ware

Acme (computer virus), a computer virus which infects DOS .EXE executable files
ACME (health software) automated determination of cause of death, used by several governments for the generation of national mortality statistics
Acme (text editor), a text editor and development environment originally for the Plan 9 from Bell Labs operating system
Acme thread form, a screw thread used in specialized applications such as vises and leadscrews
Acme zone, a biostratigraphic zone where a fossil reaches its peak abundance
Arginine catabolic mobile element, a genetic system used by bacteria to tolerate skin polyamine defense compounds
Automatic Certificate Management Environment, a protocol for communications between a certificate authority and its users' web servers
Summit or acme, a topographic term

Acme Aircraft Co, later known as Sierradyne, an aircraft manufacturer based in Torrance, California
Acme Aircraft Corporation, an aircraft manufacturer based in Rockford, Illinois, during the 1920s
Acme Boots, a manufacturer of western footwear owned by Berkshire Hathaway
Acme Bread Company, a bakery in Berkeley, California
Acme Brick, a brick manufacturer that is a subsidiary of Berkshire Hathaway
ACME Comics & Collectibles, a comics and collectibles store in Sioux City, Iowa
ACME Communications, a television broadcasting company, based in Santa Ana, California
Acme Fresh Market, a chain of grocery stores in northeast Ohio
Acme Markets, a chain of grocery stores in the northeast
Acme (automobile) (Acme Motor Car Company), an early motor car manufacturer
ACME Newspictures
Acme Packet, a VoIP Session Border Controller manufacturer
Acme Tackle Company, a producer of fishing tackle in Providence, Rhode Island
Acme Truck Line, a national transportation service based in Gretna, Louisiana
Acme United Corporation, a scissors manufacturer founded in 1867 in Naugatuck, Connecticut
Air Craft Marine Engineering, a short-lived aircraft manufacturer established in 1954

Acme Motor Co, a defunct motorcycle manufacturer based in Coventry
Acme Press Ltd., a defunct comics publisher (and retailer) in England
Acme Space, an architecture firm based in London
Acme Studios, a charity based in London
Acme Whistles, a major producer of whistles
Advisory Committee on Mathematics Education, a British organisation established in 2002 by the Royal Society and the Joint Mathematical Council
Acme Software, a former name of Rockstar North

Acme Farm Supply Building, a listed building in Nashville, Tennessee
ACME Comedy Theatre, a sketch comedy and improvisational theater located near Hollywood

Accentor-class minesweeper, a US Navy class sometimes called the Accentor/Acme class
- USS Acme (AMc-61), a coastal minesweeper launched in 1941
- USS Acme (MSO-508), a minesweeper launched in 1955
USS Abarenda (IX-131) SS Acme, original name of the, a storage tanker
Acme (1876), a schooner built in Sydney, Australia
Acme (steamboat), operated in the state of Washington from 1899 to 1910

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 * [[Acme zone]], a biostratigraphic zone where a fossil reaches its peak abundance
 * [[Arginine catabolic mobile element]], a genetic system used by bacteria to tolerate skin polyamine defense compounds
-* [[Automated Certificate Management Environment]], a protocol for communications between a certificate authority and its users' web servers
+* [[Automatic Certificate Management Environment]], a protocol for communications between a certificate authority and its users' web servers
 * [[Summit]] or acme, a topographic term