Cell culture: Difference between revisions
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===Cell line cross-contamination=== |
===Cell line cross-contamination=== |
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Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest that anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line.<ref>Drexler, H.G., Dirks,W.G. and MacLeod, R.A.F. (1999) False human hematopoetic cell lines: cross-contaminations and misinterpretations. ''Leukemia'' '''13''', 1601–1607. [http://www.ncbi.nlm.nih.gov/pubmed/10516762 PMID: 10516762.]</ref> <ref>Drexler, H.G. ''et al''. (2001) Cross-contamination: HS-Sultan is not a myeloma but a Burkitt lymphoma cell line. ''Blood'' '''98''', 3495–6. [http://www.ncbi.nlm.nih.gov/pubmed/11732505 PMID: 11732505] </ref><ref>Cabrera, C.M. ''et al''. (2006) Identity tests: Determination of cross contamination. ''Cytotechnology'' '''51''', 45–50. [http://dx.doi.org/10.1007/s10616-006-9013-8 DOI: 10.1007/s10616-006-9013-8]</ref> Problems with cell line cross contamination even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. <ref name=chatterjee>Chatterjee, R. (2007) Cell biology. Cases of mistaken identity. ''Science'' '''315''', 928–931 [http://www.ncbi.nlm.nih.gov/pubmed/17303729 PMID: 17303729]</ref> <ref>Liscovitch, M. and Ravid, D. (2007) A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived from OVCAR-8 human ovarian carcinoma cells. ''Cancer Lett''. '''245''', 350–352. [http://www.ncbi.nlm.nih.gov/pubmed/16504380 PMID: 16504380] </ref> Major cell line repositories including the American Type Culture Collection (ATCC) and the European Collection of Cell Cultures (ECACC) have received cell line submissions from researchers that were misidentified by the researcher.<ref name=chatterjee/><ref>MacLeod, R.A.F. ''et al''. (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. ''Int. J. Cancer'' '''83''', 555–563 [http://www.ncbi.nlm.nih.gov/pubmed/10508494 PMID: 10508494]</ref> Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions. ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.<ref name=dunham>Dunham, J.H. and Guthmiller, P. (2008) [http://www.promega.com/cnotes/cn022/cn022_15.pdf Doing good science: Authenticating cell line identity.] ''Cell Notes'' '''22''', 15–17.</ref> |
Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest that anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line.<ref>Drexler, H.G., Dirks,W.G. and MacLeod, R.A.F. (1999) False human hematopoetic cell lines: cross-contaminations and misinterpretations. ''Leukemia'' '''13''', 1601–1607. [http://www.ncbi.nlm.nih.gov/pubmed/10516762 PMID: 10516762.]</ref> <ref>Drexler, H.G. ''et al''. (2001) Cross-contamination: HS-Sultan is not a myeloma but a Burkitt lymphoma cell line. ''Blood'' '''98''', 3495–6. [http://www.ncbi.nlm.nih.gov/pubmed/11732505 PMID: 11732505] </ref><ref>Cabrera, C.M. ''et al''. (2006) Identity tests: Determination of cross contamination. ''Cytotechnology'' '''51''', 45–50. [http://dx.doi.org/10.1007/s10616-006-9013-8 DOI: 10.1007/s10616-006-9013-8]</ref> Problems with cell line cross contamination even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. <ref name=chatterjee>Chatterjee, R. (2007) Cell biology. Cases of mistaken identity. ''Science'' '''315''', 928–931 [http://www.ncbi.nlm.nih.gov/pubmed/17303729 PMID: 17303729]</ref> <ref>Liscovitch, M. and Ravid, D. (2007) A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived from OVCAR-8 human ovarian carcinoma cells. ''Cancer Lett''. '''245''', 350–352. [http://www.ncbi.nlm.nih.gov/pubmed/16504380 PMID: 16504380] </ref> Major cell line repositories including the American Type Culture Collection (ATCC) and the European Collection of Cell Cultures (ECACC) have received cell line submissions from researchers that were misidentified by the researcher.<ref name=chatterjee/><ref name=macleod>MacLeod, R.A.F. ''et al''. (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. ''Int. J. Cancer'' '''83''', 555–563 [http://www.ncbi.nlm.nih.gov/pubmed/10508494 PMID: 10508494]</ref> Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions.<ref>Masters, John R. (2002): HeLa cells 50 years on: the good, the bad and the ugly. ''[[Nature Reviews Cancer]]'' '''2''':315-319. See [[List of contaminated cell lines]].</ref> ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.<ref name=dunham>Dunham, J.H. and Guthmiller, P. (2008) [http://www.promega.com/cnotes/cn022/cn022_15.pdf Doing good science: Authenticating cell line identity.] ''Cell Notes'' '''22''', 15–17.</ref> |
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To address this problem of cell line cross-contamination, researchers are encouraged to authenticate their cell lines at an early passage to establish the identity of the cell line. Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. There are many methods for identifying cell lines including isoenzyme analysis, human lymphocyte antigen (HLA) typing and STR analysis.<ref name=dunham/> |
To address this problem of cell line cross-contamination, researchers are encouraged to authenticate their cell lines at an early passage to establish the identity of the cell line. Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. There are many methods for identifying cell lines including isoenzyme analysis, human lymphocyte antigen (HLA) typing and STR analysis.<ref name=dunham/> |
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[[Image:HeLa Hoechst 33258.jpg|thumb|right|300px|One of the earliest human cell lines, descended from [[Henrietta Lacks]], who died of the cancer that those cells originated from, the cultured [[HeLa]] cells shown here have been stained with [[Hoechst stain|Hoechst]] turning their [[Cell nucleus|nuclei]] blue.]] |
[[Image:HeLa Hoechst 33258.jpg|thumb|right|300px|One of the earliest human cell lines, descended from [[Henrietta Lacks]], who died of the cancer that those cells originated from, the cultured [[HeLa]] cells shown here have been stained with [[Hoechst stain|Hoechst]] turning their [[Cell nucleus|nuclei]] blue.]] |
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Cell lines that originate with [[human]]s have been somewhat controversial in [[bioethics]], as they may outlive their parent organism and later be used in the discovery of lucrative medical treatments. In the pioneering decision in this area, the [[John Moore v. the Regents of the University of California|Supreme Court of California]] held in 1990 that human patients have no property rights in cell lines derived from organs removed with their consent. <ref>[http://online.ceb.com/calcases/C3/51C3d120.htm ceb]</ref> |
Cell lines that originate with [[human]]s have been somewhat controversial in [[bioethics]], as they may outlive their parent organism and later be used in the discovery of lucrative medical treatments. In the pioneering decision in this area, the [[John Moore v. the Regents of the University of California|Supreme Court of California]] held in 1990 that human patients have no property rights in cell lines derived from organs removed with their consent. <ref>[http://online.ceb.com/calcases/C3/51C3d120.htm ceb]</ref> |
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It is estimated that about 20% of human cell lines are not the kind of cells they were generally assumed to be.<ref>(MacLeoud ''et al''. 1999)</ref> The reason for this is that some cell lines exhibit vigorous growth and thus can cross-contaminate cultures of other cell lines, in time overgrowing and displacing the original cells. The most common contaminant is the [[HeLa]] cell line. While this may not be of significance when general properties such as cell [[metabolism]] are researched, it is highly relevant e.g. in medical research focusing on a specific type of cell. Results of such research will be at least flawed, if not outright wrong in their conclusion, with possible consequences if therapeutic approaches are developed based on it. <ref>(Masters 2002). See [[List of contaminated cell lines]].</ref> |
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===Generation of hybridomas=== |
===Generation of hybridomas=== |
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Revision as of 17:27, 28 October 2008
Cell culture is the process by which prokaryotic or eukaryotic cells are grown under controlled conditions. In practice the term "cell culture" has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture.
Animal cell culture became a common laboratory technique in the 1950s,[1] but the concept of maintaining live cell lines separated from their original tissue source was discovered in the 19th century.[2]
History
The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, potassium, calcium and magnesium suitable for maintaining the beating of an isolated animal heart outside of the body.[1] In 1885 Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture.[3] Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907-1910, establishing the methodology of tissue culture.[4]
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. The Salk polio vaccine was one of the first products mass-produced using cell culture techniques. This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures.
Best book for cell culture is Freshney- Animal cell culture
Concepts in mammalian cell culture
Isolation of cells
Cells can be isolated from tissues for ex vivo culture in several ways. Cells can be easily purified from blood, however only the white cells are capable of growth in culture. Mononuclear cells can be released from soft tissues by enzymatic digestion with enzymes such as collagenase, trypsin, or pronase, which break down the extracellular matrix. Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. This method is known as explant culture.
Cells that are cultured directly from a subject are known as primary cells. With the exception of some derived from tumours, most primary cell cultures have limited lifespan. After a certain number of population doublings cells undergo the process of senescence and stop dividing, while generally retaining viability.
An established or immortalised cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. There are numerous well established cell lines representative of particular cell types.
Maintaining cells in culture
Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37°C, 5% CO2 for mammalian cells) in a cell incubator. Culture conditions vary widely for each cell type, and variation of conditions for a particular cell type can result in different phenotypes being expressed.
Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the growth medium. Recipes for growth media can vary in pH, glucose concentration, growth factors, and the presence of other nutrients. The growth factors used to supplement media are often derived from animal blood, such as calf serum. One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in biotechnology medical applications. Current practice is to minimize or eliminate the use of these ingredients wherever possible, but this cannot always be accomplished.
Cells can be grown in suspension or adherent cultures. Some cells naturally live in suspension, without being attached to a surface, such as cells that exist in the bloodstream. There are also cell lines that have been modified to be able to survive in suspension cultures so that they can be grown to a higher density than adherent conditions would allow. Adherent cells require a surface, such as tissue culture plastic, which may be coated with extracellular matrix components to increase adhesion properties and provide other signals needed for growth and differentiation. Most cells derived from solid tissues are adherent. Another type of adherent culture is organotypic culture which involves growing cells in a three-dimensional environment as opposed to two-dimensional culture dishes. This 3D culture system is biochemically and physiologically more similar to in vivo tissue, but is technically challenging to maintain because of many factors (e.g. diffusion).
Cell line cross-contamination
Cell line cross-contamination can be a problem for scientists working with cultured cells. Studies suggest that anywhere from 15–20% of the time, cells used in experiments have been misidentified or contaminated with another cell line.[5] [6][7] Problems with cell line cross contamination even been detected in lines from the NCI-60 panel, which are used routinely for drug-screening studies. [8] [9] Major cell line repositories including the American Type Culture Collection (ATCC) and the European Collection of Cell Cultures (ECACC) have received cell line submissions from researchers that were misidentified by the researcher.[8][10] Such contamination poses a problem for the quality of research produced using cell culture lines, and the major repositories are now authenticating all cell line submissions.[11] ATCC uses short tandem repeat (STR) DNA fingerprinting to authenticate its cell lines.[12]
To address this problem of cell line cross-contamination, researchers are encouraged to authenticate their cell lines at an early passage to establish the identity of the cell line. Authentication should be repeated before freezing cell line stocks, every two months during active culturing and before any publication of research data generated using the cell lines. There are many methods for identifying cell lines including isoenzyme analysis, human lymphocyte antigen (HLA) typing and STR analysis.[12]
Manipulation of cultured cells
As cells generally continue to divide in culture, they generally grow to fill the available area or volume. This can generate several issues:
- Nutrient depletion in the growth media
- Accumulation of apoptotic/necrotic (dead) cells.
- Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing known as contact inhibition or senescence.
- Cell-to-cell contact can stimulate cellular differentiation.
Among the common manipulations carried out on culture cells are media changes, passaging cells, and transfecting cells. These are generally performed using tissue culture methods that rely on sterile technique. Sterile technique aims to avoid contamination with bacteria, yeast, or other cell lines. Manipulations are typically carried out in a biosafety hood or laminar flow cabinet to exclude contaminating micro-organisms. Antibiotics can also be added to the growth media.
Media changes
In the case of adherent cultures, the media can be removed directly by aspiration and replaced.
Passaging cells
Passaging (also known as subculture or splitting cells) involves transferring a small number of cells into a new vessel. Cells can be cultured for a longer time if they are split regularly, as it avoids the senescence associated with prolonged high cell density. Suspension cultures are easily passaged with a small amount of culture containing a few cells diluted in a larger volume of fresh media. For adherent cultures, cells first need to be detached; this is commonly done with a mixture of trypsin-EDTA, however other enzyme mixes are now available for this purpose. A small number of detached cells can then be used to seed a new culture.
Transfection and transduction
Another common method for manipulating cells involves the introduction of foreign DNA by transfection. This is often performed to cause cells to express a protein of interest. More recently, the transfection of RNAi constructs have been realized as a convenient mechanism for suppressing the expression of a particular gene/protein.
DNA can also be inserted into cells using viruses, in methods referred to as transduction, infection or transformation. Viruses, as parasitic agents, are well suited to introducing DNA into cells, as this is a part of their normal course of reproduction.
Established human cell lines
Cell lines that originate with humans have been somewhat controversial in bioethics, as they may outlive their parent organism and later be used in the discovery of lucrative medical treatments. In the pioneering decision in this area, the Supreme Court of California held in 1990 that human patients have no property rights in cell lines derived from organs removed with their consent. [13]
Generation of hybridomas
It is possible to fuse normal cells with an immortalised cell line. This method is used to produce monoclonal antibodies. In brief, lymphocytes isolated from the spleen (or possibly blood) of an immunised animal are combined with an immortal myeloma cell line (B cell lineage) to produce a hybridoma which has the antibody specifity of the primary lymphoctye and the immortality of the myleoma. Selective growth medium (HA or HAT) is used to select against unfused myeloma cells; primary lymphoctyes die quickly in culture and only the fused cells survive. These are screened for production of the required antibody, generally in pools to start with and then after single cloning.
Applications of cell culture
Mass culture of animal cell lines is fundamental to the manufacture of viral vaccines and many products of biotechnology. Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes, synthetic hormones, immunobiologicals (monoclonal antibodies, interleukins, lymphokines), and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures, more complex proteins that are glycosylated (carbohydrate-modified), currently must be made in animal cells. An important example of such a complex protein is the hormone erythropoietin. The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants.
Tissue culture and engineering
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells ex vivo.
Vaccines
Vaccines for polio, measles, mumps, rubella, and chickenpox are currently made in cell cultures. Due to the H5N1 pandemic threat, research into using cell culture for influenza vaccines is being funded by the United States government. Novel ideas in the field include recombinant DNA-based vaccines, such as one made using human adenovirus (a common cold virus) as a vector,[14] [15] or the use of adjuvants. [16]
Culture of non-mammalian cells
Plant cell culture methods
Plant cell cultures are typically grown as cell suspension cultures in liquid medium or as callus cultures on solid medium. The culturing of undifferentiated plant cells and calli requires the proper balance of the plant growth hormones auxin and cytokinin.
Bacterial/Yeast culture methods
For bacteria and yeast, small quantities of cells are usually grown on a solid support that contains nutrients embedded in it, usually a gel such as agar, while large-scale cultures are grown with the cells suspended in a nutrient broth.
Viral culture methods
The culture of viruses requires the culture of cells of mammalian, plant, fungal or bacterial origin as hosts for the growth and replication of the virus. Whole wild type viruses, recombinant viruses or viral products may be generated in cell types other than their natural hosts under the right conditions. Depending on the species of the virus, infection and viral replication may result in host cell lysis and formation of a viral plaque.
Common cell lines
- Human cell lines
- National Cancer Institute's 60 cancer cell lines
- ESTDAB database http://www.ebi.ac.uk/ipd/estdab/directory.html
- DU145 (Prostate cancer)
- Lncap (Prostate cancer)
- MCF-7 (breast cancer)
- MDA-MB-438 (breast cancer)
- PC3 (Prostate cancer)
- T47D ([breast cancer])
- THP-1 (acute myeloid leukemia)
- U87 (glioblastoma)
- SHSY5Y Human neuroblastoma cells, cloned from a myeloma
- Saos-2 cells (bone cancer)
- Primate cell lines
- Vero (African green monkey Chlorocebus kidney epithelial cell line initiated 1962)
- Rat tumor cell lines
- Mouse cell lines
- Plant cell lines
- Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell)
- Other species cell lines
- zebrafish ZF4 and AB9 cells.
- Madin-Darby Canine Kidney (MDCK) epithelial cell line
- Xenopus A6 kidney epithelial cells.
List of cell lines
| Cell line | Meaning | Organism | Origin tissue | Morphology | Link |
|---|---|---|---|---|---|
| 293-T | Human | kidney (embryonic) | Derivative of HEK 293 | ||
| 3T3 cells | "3-day transfer, inoculum 3 x 105 cells" | Mouse | embryonic fibroblast | Also known as NIH 3T3 | |
| 721 | Human | melanoma | |||
| 9L | Rat | glioblastoma | |||
| A172 | human | glioblastoma | malignant glioma | ||
| A20 | murine | B lymphoma | B lymphocyte | ||
| A253 | human | head and neck carcinoma | submandibular duct | ||
| A431 | human | skin epithelium | squamous carcinoma | Biotech insitute | |
| A-549 | human | lungcarcinoma | epithelium | DSMZ | |
| ALC | murine | bone marrow | stroma | NCBI | |
| B16 | murine | Melanoma | |||
| B35 | rat | Neuroblastoma | ATCC | ||
| BCP-1 cells | Human | PBMC | HIV+ lymphoma | ATCC | |
| bEnd.3 | brain endothelial | mouse | brain / cerebral Cortex | endothelium | ATCC |
| BHK-21 | "Baby Hamster Kidney Fibroblast cells" | Hamster | kidney | fibroblast | Olympus |
| BR 293 | human | breast | breast cancer | ||
| BxPC3 | Biopsy xenograph of pancreatic carcinoma line 3 | human | pancreatic adenocarcinoma | epithelial | ATCC |
| C3H-10T1/2 | Mouse | Embryonic mesenchymal cell line | |||
| C6/36 | Asian tiger mosquito | larval tissue | |||
| Cal-27 | human | tongue | squamous cell carcinoma | Biotech institute | |
| CHO | Chinese hamster ovary | hamster | Ovary | epithelium | - |
| COS-7 | Cercopithecus aethiops, origin-defective SV-40 | ape - Cercopithecus aethiops (Chlorocebus) | kidney | fibroblast | ATCC |
| CML T1 | Chronic Myelod Leukaemia T-lymphocyte 1 | human | CML acute phase | T cell leukaemia | Blood |
| CMT | canine mammary tumor | dog | mammary gland | epithelium | |
| CT26 | murine | Colorectal Carcinoma | Colon | ||
| D17 | canine | osteosarcoma | |||
| DH82 | canine | histiocytosis | monocyte/macrophage | J Vir Meth | |
| DU145 | human | Androgen insensitive carcinoma | Prostate | ||
| EL4 | mouse | T cell leukaemia | |||
| EM2 | human | CML blast crisis | Ph+ CML line | Biotech institute | |
| EM3 | human | CML blast crisis | Ph+ CML line | Biotech institute | |
| FM3 | human | Metastatic lymph node | melanoma | Biotech institute | |
| H1299 | human | lung | lung cancer | ||
| HB54 | hybridoma | hybridoma | secretes L243 mAb (against HLA-DR) | Human Immunology | |
| HB55 | hybridoma | hybridoma | secretes MA2.1 mAb (against HLA-A2 and HLA-B17) | Journal of Immunology | |
| HCA2 | human | fibroblast | Journal of General Virology | ||
| HEK-293 | human embryonic kidney | human | kidney (embryonic) | epithelium | ATCC |
| HeLa | Henrietta Lacks | human | Cervical cancer | epithelium | DSMZ |
| Hepa1c1c7 | clone 7 of clone 1 hepatoma line 1 | mouse | Hepatoma | epithelial | ATCC |
| HL-60 | human leukemia | human | Myeloblast | bloodcells | DSMZ |
| HMEC | human mammary epithelial cell | human | epithelium | ||
| HT-29 | human | colon epithelium | adenocarcinoma | Biotech Institute | |
| HUVEC | human umbilical vein endothelial cells | human | Umbilical cord vein | endothelium | ICLC |
| Jurkat | human | T-Cell-Leukemia | white blood cells | DSMZ | |
| JY cells | human | lymphoblastoid | EBV immortalised B cell | ||
| K562 cells | human | lymphoblastoid | CML blast crisis | ||
| K812 | human | lymphoblastoid | erythroleukemia | ||
| KCL22 | human | lymphoblastoid | CML | ||
| KG1 | human | lymphoblastoid | AML | ||
| KYO1 | Kyoto 1 | human | lymphoblastoid | CML | DSMZ |
| LNCap | human | prostatic adenocarcinoma | epithelium | ATCC | |
| Ma-Mel 1, 2, 3....48 | human | a range of melanoma cell lines | |||
| MC-38 | mouse | adenocarcinoma | |||
| MCF-10A | Michigan Cancer Foundation | human | mammary gland | epithelium | ATCC |
| MDA-231 | human | breast | cancer | ||
| MDA-468 | human | breast | cancer | ||
| MDA-MB-435 | human | breast | melanoma or carcinoma (disputed) | Cambridge Pathology | |
| MDCK II | Madin Darby canine kidney | dog | kidney | epithelium | ATCC |
| MDCK II | Madin Darby canine kidney | dog | kidney | epithelium | ATCC |
| MONO-MAC 6 | human | WBC | myeloid metaplasic AML | Biotech Institute | |
| MTD-1A | mouse | epithelium | |||
| MyEnd | myocardial endothelial | mouse | endothelium | ||
| NIH-3T3 | NIH, 3-day transfer, inoculum 3 x 105 cells | mouse | embryo | fibroblast | ATCC |
| NALM-1 | peripheral blood | blast-crisis CML | Cancer Genetics and Cytogenetics | ||
| NW-145 | melanoma | ESTDAB | |||
| OPCN / OPCT cell lines | Onyvax[2] Prostate Cancer.... | Range of prostate tumour lines | Asterand | ||
| Peer | human | T cell leukemia | DSMZ | ||
| PNT-1A / PNT 2 | Prostate tumour lines | ||||
| RenCa | Renal Carcinoma | mouse | renal carcinoma | ||
| RMA/RMAS | mouse | T cell tumour | |||
| Saos-2 cells | human | Osteosarcoma | |||
| Sf-9 | Spodoptera frugiperda | insect - Spodoptera frugiperda (moth) | Ovary | DSMZ | |
| SkBr3 | human | breast carcinoma | |||
| T2 | human | T cell leukemia/B cell line hybridoma | DSMZ | ||
| T84 | human | colorectal Carcinoma / lungmetastasis | epithelium | ATCC | |
| THP1 cell line | human | monocyte | AML | ||
| U373 | human | glioblastoma-astrocytoma | epithelium | ||
| U87 | human | glioblastoma-astrocytoma | epithelial-like | Abcam | |
| U937 | human | leukaemic monocytic lymphoma | |||
| Vero cells | 'Vera Redno' ('green kidney') / 'Vero' ('truth') | African Green Monkey | kidney epithelium | ||
| WM39 | human | skin | primary melanoma | ||
| WT-49 | human | lymphoblastoid | |||
| X63 | mouse | melanoma | |||
| YAC-1 | mouse | lymphoma | Biotech Institute | ||
| YAR | human | B-cell | EBV transofrmed | Human Immunology |
Note: this list is a sample of available cell lines, and is not comprehensive
See also
- Biological immortality
- Cell culture assays
- List of contaminated cell lines
- Organ culture
- Plant tissue culture
- Tissue culture
References and notes
- ^ ""Cell Culture"". Retrieved 2006-04-19.
- ^ ""Some landmarks in the development of tissue and cell culture."". Retrieved 2006-04-19.
- ^ ""Animals and alternatives in testing."". Retrieved 2006-04-19.
- ^ Schiff, Judith Ann. ""An unsung hero of medical research."". Retrieved 2006-04-19. Yale Alumni Magazine, February 2002.
- ^ Drexler, H.G., Dirks,W.G. and MacLeod, R.A.F. (1999) False human hematopoetic cell lines: cross-contaminations and misinterpretations. Leukemia 13, 1601–1607. PMID: 10516762.
- ^ Drexler, H.G. et al. (2001) Cross-contamination: HS-Sultan is not a myeloma but a Burkitt lymphoma cell line. Blood 98, 3495–6. PMID: 11732505
- ^ Cabrera, C.M. et al. (2006) Identity tests: Determination of cross contamination. Cytotechnology 51, 45–50. DOI: 10.1007/s10616-006-9013-8
- ^ a b Chatterjee, R. (2007) Cell biology. Cases of mistaken identity. Science 315, 928–931 PMID: 17303729
- ^ Liscovitch, M. and Ravid, D. (2007) A case study in misidentification of cancer cell lines: MCF-7/AdrR cells (re-designated NCI/ADR-RES) are derived from OVCAR-8 human ovarian carcinoma cells. Cancer Lett. 245, 350–352. PMID: 16504380
- ^ MacLeod, R.A.F. et al. (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 83, 555–563 PMID: 10508494
- ^ Masters, John R. (2002): HeLa cells 50 years on: the good, the bad and the ugly. Nature Reviews Cancer 2:315-319. See List of contaminated cell lines.
- ^ a b Dunham, J.H. and Guthmiller, P. (2008) Doing good science: Authenticating cell line identity. Cell Notes 22, 15–17.
- ^ ceb
- ^ cold as vector news
- ^ cold as vector research abstract
- ^ adjuvant
- MacLeod, R. A. F. et al. (1999): Widespread intraspecies cross-contamination of human tumour cell lines. International Journal of Cancer 83:555–563.
- Masters, John R. (2002): HeLa cells 50 years on: the good, the bad and the ugly. Nature Reviews Cancer 2:315-319.
External links
- Health Protection Agency Culture Collections - including ECACC (European Collection of Cell Cultures)
- Witkowski JA. Experimental pathology and the origins of tissue culture: Leo Loeb's contribution. Med Hist. 1983 July; 27(3): 269–288.
- atcc
- Coriell Cell Repositories
- The National Centre for Cell Science (NCCS), Pune, India; national repository for cell lines/hybridomas etc.
- Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation (a protocol)
- Rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays (a protocol)
- Table of common cell lines from Alberts 4th ed.