Depurination: Difference between revisions
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'''Depurination''' is a [[DNA]] alteration in which the hydrolysis of the beta-N-glycocidic link between a [[purine]] base (Adenine or Guanine) and the [[deoxyribose]]-[[phosphate]] backbone occurs. After a depurination, the sugar phosphate backbone remains and the sugar ring has a [[hydroxyl]] (-OH) group in the place of the Adenine or Guanine. Studies estimate that as many as 10,000 Purines are lost this way each day in a typical human cell, however this represents only 1/30,000 of the human genome.<ref name=instability> Lindahl T. (1993) ''[[Instability and decay of the primary structure of DNA.]]'' Nature 103; 362:709-715; {{}} </ref> One of the main causes of depurination is the presence of endogenous metabolites in cell undergoing chemical reactions. This breaks the bond linking the purine with the pentose sugar. Depurinations are efficiently repaired by portions of the [[ |
'''Depurination''' is a [[DNA]] alteration in which the hydrolysis of the beta-N-glycocidic link between a [[purine]] base (Adenine or Guanine) and the [[deoxyribose]]-[[phosphate]] backbone occurs. After a depurination, the sugar phosphate backbone remains and the sugar ring has a [[hydroxyl]] (-OH) group in the place of the Adenine or Guanine. Studies estimate that as many as 10,000 Purines are lost this way each day in a typical human cell, however this represents only 1/30,000 of the human genome.<ref name=instability> Lindahl T. (1993) ''[[Instability and decay of the primary structure of DNA.]]'' Nature 103; 362:709-715; {{}} </ref> One of the main causes of depurination is the presence of endogenous metabolites in cell undergoing chemical reactions. This breaks the bond linking the purine with the pentose sugar. Depurinations are efficiently repaired by portions of the [[Base excision repair]] (BER) pathway, and very few depurinations lead to mutations. |
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N.B. depurination can occur on pyrimidine bases also, but at a substantially lower rate |
N.B. depurination can occur on pyrimidine bases also, but at a substantially lower rate |
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Revision as of 17:20, 9 April 2009
Depurination is a DNA alteration in which the hydrolysis of the beta-N-glycocidic link between a purine base (Adenine or Guanine) and the deoxyribose-phosphate backbone occurs. After a depurination, the sugar phosphate backbone remains and the sugar ring has a hydroxyl (-OH) group in the place of the Adenine or Guanine. Studies estimate that as many as 10,000 Purines are lost this way each day in a typical human cell, however this represents only 1/30,000 of the human genome.[1] One of the main causes of depurination is the presence of endogenous metabolites in cell undergoing chemical reactions. This breaks the bond linking the purine with the pentose sugar. Depurinations are efficiently repaired by portions of the Base excision repair (BER) pathway, and very few depurinations lead to mutations.
N.B. depurination can occur on pyrimidine bases also, but at a substantially lower rate
References
- ^ Lindahl T. (1993) Instability and decay of the primary structure of DNA. Nature 103; 362:709-715; {{}}
Hartwell, Hood, Goldberg, Reynolds, Silver, Veres. Genetics: From Genes to Genomes. Second Edition. McGraw-Hill Companies Inc. New York, NY: 2004.
Weinberg. The Biology of Cancer. First Edition. Garland Science. 2006
Alberts, Johnson, Lewis, Raff, Roberts, Walter. "Molecular Biology of the Cell". Fourth Edition. Garland Science. New York, NY: 2002.
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