Poly(A)-binding protein: Difference between revisions
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#REDIRECT [[PABPC1]] |
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[[Image:Poly(A)-binding protein.png|thumb|right|300px|Poly(A) RNA Binding Protein PABP, PDB 1CVJ]] |
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'''Poly(A)-binding protein''' is a [[RNA-binding protein]] which binds to the [[poly(A) tail]] of mRNA. The poly(A) tail is located on the 3' end of mRNA. The nuclear [[isoforms]] selectively binds to around 50 nucleotides and stimulates the activity of [[Polyadenylate polymerase]]. |
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The expression of mammalian Poly(A)-binding protein is regulated at the translational level by a feed-back mechanism: the mRNA encoding PABP contains in its 5' [[UTR]] an A-rich sequence which binds Poly(A)-binding protein. This leads to repression of translation |
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The cytosolic isoform of eukaryotes Poly(A) binding protein binds to the initiation factor [[eIF-4G]] via its C-terminal domain. EIF-4G is bound to [[eIF-4E]], another initiation factor bound to the [[5' cap]] on the 5' end of mRNA. This binding forms the characteristic loop structure of eukaryotic protein synthesis. Poly(A)-binding protein interacting proteins in the cytosol compete for the eIF-4G binding sites. Poly(A)-binding protein has also been shown to interact with a termination factor ([[eRF3]]) |
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==Rotavirus NSP3== |
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[[Rotavirus]] [[RNA-binding protein]] [[NSP3(Rotavirus)|NSP3]] interacts with [[Eukaryotic initiation factor|eIF4GI]] and evicts the poly(A) binding protein from [[Eukaryotic initiation factor|eIF4F]]. And NSP3A, by taking the place of PABP on [[Eukaryotic initiation factor|eIF4GI]], is responsible for the shut-off of cellular protein synthesis.<ref name=pmid9755181>{{Cite journal |unused_data=DUPLICATE DATA: doi=10.1093/emboj/17.19.5811 |pmid=9755181 |doi=10.1093/emboj/17.19.5811 |year=1998 |last1=Piron |first1=M |last2=Vende |last3=Cohen |last4=Poncet |title=Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F |volume=17 |issue=19 |pages=5811–21 |journal=The EMBO journal |format=Free full text |first2=P |first3=J |first4=D |pmc=1170909}}</ref> |
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[[Image:Rotavirus Translation.svg|thumb|200px|center|Cellular vs Rotavirus Translation]] |
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==Genes== |
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* ''[[PABPC1]]'', ''[[PABPC3]]'', ''[[PABPC4]]'', {{Gene|PABPC5}} |
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==References== |
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{{Reflist}} |
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==External links== |
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* {{MeshName|Poly(A)-Binding+Proteins}} |
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{{Biochem-stub}} |
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{{RNA-binding proteins}} |
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[[Category:RNA-binding proteins]] |
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Revision as of 17:17, 18 December 2010
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