Bile esculin agar: Difference between revisions
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==Uses== |
==Uses== |
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Bile Esculin Agar is used primarily to differentiate ''[[Enterococcus]]'' from ''[[Streptococcus]]''. Members of the genus [[Enterococcus]] are capable of growing in the presence of 4% bile (oxgall) and hydrolyzing esculin to glucose and esculetin. Esculetin combines with ferric ions to produce a black complex. |
Bile Esculin Agar is used primarily to differentiate ''[[Enterococcus]]'' from ''[[Streptococcus]]''. Members of the genus [[Enterococcus]] are capable of growing in the presence of 4% bile (oxgall) and hydrolyzing esculin to [[glucose]] and [[esculetin]]. Esculetin combines with ferric ions to produce a black complex. |
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For some purposes, certain bacteria are able to hydrolyze [[aesculin]]. A plate containing aesculin will fluoresce a pale blue under UV radiation. Some bacteria can hydrolise this, leading to UV '''dark''' colonies, as opposed to UV '''light''' ones. |
For some purposes, certain bacteria are able to hydrolyze [[aesculin]]. A plate containing aesculin will fluoresce a pale blue under UV radiation. Some bacteria can hydrolise this, leading to UV '''dark''' colonies, as opposed to UV '''light''' ones. |
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Revision as of 19:33, 23 September 2011
Bile Esculin Agar (BEA) is a selective differential agar used to isolate and identify members of the genus Enterococcus,[1] also known as "group D streptococci".[2]
Composition and process
Bile salts are the selective ingredient, while esculin is the differential component. Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium.
Test results must be interpreted in conjunction with gram stain morphology.
Uses
Bile Esculin Agar is used primarily to differentiate Enterococcus from Streptococcus. Members of the genus Enterococcus are capable of growing in the presence of 4% bile (oxgall) and hydrolyzing esculin to glucose and esculetin. Esculetin combines with ferric ions to produce a black complex.
For some purposes, certain bacteria are able to hydrolyze aesculin. A plate containing aesculin will fluoresce a pale blue under UV radiation. Some bacteria can hydrolise this, leading to UV dark colonies, as opposed to UV light ones.
When new techniques are produced to identify enterococci, they are often compared to the use of bile esculin agar.[3]
References
- ^ "BSCI424 Laboratory Media". Retrieved 2008-11-18.
- ^ Lindell SS, Quinn P (1975). "Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae". J. Clin. Microbiol. 1 (5): 440–3. PMC 275140. PMID 1176613.
{{cite journal}}: Unknown parameter|month=ignored (help) - ^ Grabsch EA, Ghaly-Derias S, Gao W, Howden BP (2008). "Comparative study of selective chromogenic (chromID VRE) and bile esculin agars for isolation and identification of vanB-containing vancomycin-resistant enterococci from feces and rectal swabs". J. Clin. Microbiol. 46 (12): 4034–6. doi:10.1128/JCM.00944-08. PMC 2593271. PMID 18832121.
{{cite journal}}: Unknown parameter|month=ignored (help)CS1 maint: multiple names: authors list (link)
External links