Jurkat cells: Difference between revisions
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'''Jurkat cells''' (pronounced yūr′kat) are an [[Biological_immortality#Cell_lines|immortalized line]] of [[T lymphocyte]] cells that are used to study acute T cell [[leukemia]], [[Cell signaling|T cell signaling]], and the expression of various [[chemokine receptors]] susceptible to viral entry, particularly [[HIV]]. Jurkat cells are also useful in science because of their ability to produce [[interleukin 2]]. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation. |
'''Jurkat cells''' (pronounced yūr′kat) are an [[Biological_immortality#Cell_lines|immortalized line]] of [[T lymphocyte]] cells that are used to study acute T cell [[leukemia]], [[Cell signaling|T cell signaling]], and the expression of various [[chemokine receptors]] susceptible to viral entry, particularly [[HIV]]. Jurkat cells are also useful in science because of their ability to produce [[interleukin 2]]. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation. |
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The Jurkat cell line (originally called JM) was established in the late 1970s from the peripheral blood of a 14 year old boy with T cell leukemia. |
The Jurkat cell line (originally called JM) was established in the late 1970s from the peripheral blood of a 14 year old boy with T cell leukemia.<ref>{{cite journal |author=Schneider U, Schwenk H, Bornkamm G |title=Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma |journal=Int J Cancer |volume=19 |issue=5 |pages=621–6 |year=1977 |pmid=68013 |doi=10.1002/ijc.2910190505}}</ref> Different derivatives of the Jurkat cell line can now be obtained from cell culture banks<ref>[http://www.atcc.org/ American Type Culture Collection (ATCC)]</ref> that have been mutated to lack certain genes. |
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==Examples of derivatives== |
==Examples of derivatives== |
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==Cell line contamination== |
==Cell line contamination== |
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Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) that could potentially affect experimental outcomes and infect lab technicians. This infection may also change the virulence and tropism of the virus by way of phenotypic mixing and/or recombination.<ref>{{cite journal |pmc= 2593302 |year=2008 |month=Dec |last1=Takeuchi |first1=Y |last2= McClure |last3= Pizzato |title=Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research |volume=82 |issue=24 |pages=12585–12588 |journal=Journal of Virology |doi=10.1128/JVI.01726-08 |first2=MO |first3=M |pmid=18842727}}</ref> |
Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) that could potentially affect experimental outcomes and infect lab technicians. This infection may also change the virulence and tropism of the virus by way of phenotypic mixing and/or recombination.<ref>{{cite journal |pmc= 2593302 |year=2008 |month=Dec |last1=Takeuchi |first1=Y |last2= McClure |last3= Pizzato |title=Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research |volume=82 |issue=24 |pages=12585–12588 |journal=Journal of Virology |doi=10.1128/JVI.01726-08 |first2=MO |first3=M |pmid=18842727}}</ref> |
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==External links== |
==External links== |
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*{{MeshName|Jurkat Cells}} |
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[[Category:Cell lines]] |
[[Category:Cell lines]] |
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Revision as of 20:35, 7 August 2012
Jurkat cells (pronounced yūr′kat) are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
The Jurkat cell line (originally called JM) was established in the late 1970s from the peripheral blood of a 14 year old boy with T cell leukemia.[1] Different derivatives of the Jurkat cell line can now be obtained from cell culture banks[2] that have been mutated to lack certain genes.
Examples of derivatives
- The JCaM1.6 cell line is deficient in Lck kinase activity due to the deletion of part of the lck gene (exon 7) from the Lck transcript.
- J.RT3-T3.5 cells have a mutation in the T cell receptor beta chain locus precluding expression of this chain. This affects the cells in several ways. They do not express surface CD3 or produce the T cell receptor alpha/beta heterodimer. Since they are deficient in the TCR complex, these cells are a useful tool for transfection studies using T cell receptor alpha and beta chain genes and are widely used in labs in which T cell receptor gene transfer technologies are studied.
- The I 9.2 and I 2.1 cell lines. The I 2.1 cell line is functionally defective for FADD and the I 9.2 cell line is functionally defective for caspase-8, both defective molecules being essential to apoptosis or programmed cell death of cells.
- The D1.1 cell line does not express CD4 molecule, an important co-receptor in the activation pathway of helper T cells.
- The J.gamma1 subline contains no detectable phospholipase C-gamma1 (PLC-γ1) protein and therefore has profound defects in T cell receptor (TCR) calcium mobilization, and nuclear factor of activated T-cells (NFAT) activation (an important transcription factor in T cells).
Cell line contamination
Jurkat J6 cells have been found to produce a xenotropic murine leukemia virus (X-MLV) that could potentially affect experimental outcomes and infect lab technicians. This infection may also change the virulence and tropism of the virus by way of phenotypic mixing and/or recombination.[3]
References
- ^ Schneider U, Schwenk H, Bornkamm G (1977). "Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma". Int J Cancer. 19 (5): 621–6. doi:10.1002/ijc.2910190505. PMID 68013.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ American Type Culture Collection (ATCC)
- ^ Takeuchi, Y; McClure, MO; Pizzato, M (2008). "Identification of Gammaretroviruses Constitutively Released from Cell Lines Used for Human Immunodeficiency Virus Research". Journal of Virology. 82 (24): 12585–12588. doi:10.1128/JVI.01726-08. PMC 2593302. PMID 18842727.
{{cite journal}}: Unknown parameter|month=ignored (help)
External links
- Jurkat Cells at the U.S. National Library of Medicine Medical Subject Headings (MeSH)