Trizol: Difference between revisions
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TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature. |
TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature. |
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When used, it resembles cough syrup, bright pink. The smell of the [[phenol]] and guanidinium thiocyanate is extremely strong. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components. |
When used, it resembles cough syrup, bright pink. The smell of the [[phenol]] and guanidinium thiocyanate is extremely strong and reminiscent of a brown onion. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components. |
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It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIzol (specifically [[phenol]]) and [[Chloroform]]. |
It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIzol (specifically [[phenol]]) and [[Chloroform]]. |
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Revision as of 13:16, 8 August 2012
TRIzol is a chemical solution used in RNA/DNA/protein extraction, by the reference paper from Piotr Chomczyński and Sacchi, N. in 1987.[1]
TRIzol is the brand name of the product from Ambion part of Life Technologies,[2] and the brand name from MRC,[3] which was founded by Chomczynski, is TRI Reagent.
The correct name of the method is Guanidinium thiocyanate-phenol-chloroform extraction. The use of TRIzol can result in DNA and RNA yields comparable to other extraction methods. An alternative method for RNA extraction is phenol extraction and TCA/acetone precipitation. Chloroform be exchanged with 1-bromo-3-chloropropane when using the new generation TRI Reagent.
TRIzol is light sensitive and is often stored in a dark-colored, glass container covered in foil. It must be kept below room temperature.
When used, it resembles cough syrup, bright pink. The smell of the phenol and guanidinium thiocyanate is extremely strong and reminiscent of a brown onion. TRIzol works by maintaining RNA integrity during tissue homogenization, while at the same time disrupting and breaking down cells and cell components.
It reacts strongly with chloroform and is thus used in this form of RNA extraction. Caution should be taken while using TRIzol (specifically phenol) and Chloroform.
Hazards
Exposure to TRIzol can be a serious health hazard. While mild exposure to skin can cause irritation and loss of sensation, overexposure can lead to respiratory arrest, seizure and death.[4][5]
References
- ^ Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156-9.
- ^ Life Technologies
- ^ Molecular Research Center, Inc.
- ^ Material Safety data sheet from Life technologies.
- ^ Material Safety data sheet from MRC
External links
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