Jump to content

Stichodactyla toxin: Difference between revisions

From Wikipedia, the free encyclopedia
Content deleted Content added
Gchandy (talk | contribs)
m Kv1.6 and KCa3.1/KCNN4 are also blocked by ShK. <ref></ref>
Gchandy (talk | contribs)
Line 24: Line 24:


== Target ==
== Target ==
ShK toxin blocks the K<sup>+</sup> channels K<sub>v</sub>1.1, K<sub>v</sub>1.3, K<sub>v</sub>1.6 and K<sub>v</sub>3.2<ref name="Kalman_1998">{{cite journal | author = Kalman K, Pennington MW, Lanigan MD, Nguyen A, Rauer H, Mahnir V, Paschetto K, Kem WR, Grissmer S, Gutman GA, Christian EP, Cahalan MD, Norton RS, Chandy KG | title = ShK-Dap22, a potent K<sub>v</sub>1.3-specific immunosuppressive polypeptide | journal = J. Biol. Chem. | volume = 273 | issue = 49 | pages = 32697–707 | year = 1998 | month = December | pmid = 9830012 | doi = 10.1074/jbc.273.49.32697| url = }}</ref><ref name="Rauer_1999">{{cite journal | author = Rauer H, Pennington M, Cahalan M, Chandy KG | title = Structural conservation of the pores of calcium-activated and voltage-gated potassium channels determined by a sea anemone toxin | journal = J. Biol. Chem. | volume = 274 | issue = 31 | pages = 21885–92 | year = 1999 | month = July | pmid = 10419508 | doi = 10.1074/jbc.274.31.21885| url = }}</ref><ref name="Middleton_2003">{{cite journal | author = Middleton RE, Sanchez M, Linde AR, Bugianesi RM, Dai G, Felix JP, Koprak SL, Staruch MJ, Bruguera M, Cox R, Ghosh A, Hwang J, Jones S, Kohler M, Slaughter RS, McManus OB, Kaczorowski GJ, Garcia ML | title = Substitution of a single residue in Stichodactyla helianthus peptide, ShK-Dap22, reveals a novel pharmacological profile | journal = Biochemistry | volume = 42 | issue = 46 | pages = 13698–707 | year = 2003 | month = November | pmid = 14622016 | doi = 10.1021/bi035209e | url = }}</ref><ref name="Beeton_2005">{{cite journal | author = Beeton C, Pennington MW, Wulff H, Singh S, Nugent D, Crossley G, Khaytin I, Calabresi PA, Chen CY, Gutman GA, Chandy KG | title = Targeting effector memory T cells with a selective peptide inhibitor of K<sub>v</sub>1.3 channels for therapy of autoimmune diseases | journal = Mol. Pharmacol. | volume = 67 | issue = 4 | pages = 1369–81 | year = 2005 | month = April | pmid = 15665253 | doi = 10.1124/mol.104.008193 | url = }}</ref><ref name="Yan_2005">{{cite journal | author = Yan L, Herrington J, Goldberg E, Dulski PM, Bugianesi RM, Slaughter RS, Banerjee P, Brochu RM, Priest BT, Kaczorowski GJ, Rudy B, Garcia ML | title = Stichodactyla helianthus peptide, a pharmacological tool for studying K<sub>v</sub>3.2 channels | journal = Mol. Pharmacol. | volume = 67 | issue = 5 | pages = 1513–21 | year = 2005 | month = May | pmid = 15709110 | doi = 10.1124/mol.105.011064 | url = }}</ref> The peptide binds to all four subunits in the K<sub>v</sub>1.3 tetramer through its interaction with the shallow vestibule at the outer entrance of the ion conduction pathway.<ref name="Kalman_1998"/><ref name="Rauer_1999"/><ref name="Lanigan_2002">{{cite journal | author = Lanigan MD, Kalman K, Lefievre Y, Pennington MW, Chandy KG, Norton RS | title = Mutating a critical lysine in ShK toxin alters its binding configuration in the pore-vestibule region of the voltage-gated potassium channel, K<sub>v</sub>1.3 | journal = Biochemistry | volume = 41 | issue = 40 | pages = 11963–71 | year = 2002 | month = October | pmid = 12356296| doi = 10.1021/bi026400b }}</ref> The peptide's Lysine<sup>22</sup> residue occludes the channel pore like a "cork in a bottle". This blocks the entrance to the pore.<ref name="Chandy_2004">{{cite journal | author = Chandy KG, Wulff H, Beeton C, Pennington M, Gutman GA, Cahalan MD | title = K<sup>+</sup> channels as targets for specific immunomodulation | journal = Trends Pharmacol. Sci. | volume = 25 | issue = 5 | pages = 280–9 | year = 2004 | month = May | pmid = 15120495 | doi = 10.1016/j.tips.2004.03.010 | url = | pmc = 2749963 }}</ref><ref name="Norton_2004">{{cite journal | author = Norton RS, Pennington MW, Wulff H | title = Potassium channel blockade by the sea anemone toxin ShK for the treatment of multiple sclerosis and othfer autoimmune diseases | journal = Curr. Med. Chem. | volume = 11 | issue = 23 | pages = 3041–52 | year = 2004 | month = December | pmid = 15578998 | doi = | url = http://www.bentham-direct.org/pages/content.php?CMC/2004/00000011/00000023/0003C.SGM | issn = }}</ref>
ShK toxin blocks the K<sup>+</sup> channels K<sub>v</sub>1.1, K<sub>v</sub>1.3, K<sub>v</sub>1.6, K<sub>v</sub>3.2 and K<sub>Ca<3.1,<ref name="Kalman_1998">{{cite journal | author = Kalman K, Pennington MW, Lanigan MD, Nguyen A, Rauer H, Mahnir V, Paschetto K, Kem WR, Grissmer S, Gutman GA, Christian EP, Cahalan MD, Norton RS, Chandy KG | title = ShK-Dap22, a potent K<sub>v</sub>1.3-specific immunosuppressive polypeptide | journal = J. Biol. Chem. | volume = 273 | issue = 49 | pages = 32697–707 | year = 1998 | month = December | pmid = 9830012 | doi = 10.1074/jbc.273.49.32697| url = }}</ref><ref name="Rauer_1999">{{cite journal | author = Rauer H, Pennington M, Cahalan M, Chandy KG | title = Structural conservation of the pores of calcium-activated and voltage-gated potassium channels determined by a sea anemone toxin | journal = J. Biol. Chem. | volume = 274 | issue = 31 | pages = 21885–92 | year = 1999 | month = July | pmid = 10419508 | doi = 10.1074/jbc.274.31.21885| url = }}</ref><ref name="Middleton_2003">{{cite journal | author = Middleton RE, Sanchez M, Linde AR, Bugianesi RM, Dai G, Felix JP, Koprak SL, Staruch MJ, Bruguera M, Cox R, Ghosh A, Hwang J, Jones S, Kohler M, Slaughter RS, McManus OB, Kaczorowski GJ, Garcia ML | title = Substitution of a single residue in Stichodactyla helianthus peptide, ShK-Dap22, reveals a novel pharmacological profile | journal = Biochemistry | volume = 42 | issue = 46 | pages = 13698–707 | year = 2003 | month = November | pmid = 14622016 | doi = 10.1021/bi035209e | url = }}</ref><ref name="Beeton_2005">{{cite journal | author = Beeton C, Pennington MW, Wulff H, Singh S, Nugent D, Crossley G, Khaytin I, Calabresi PA, Chen CY, Gutman GA, Chandy KG | title = Targeting effector memory T cells with a selective peptide inhibitor of K<sub>v</sub>1.3 channels for therapy of autoimmune diseases | journal = Mol. Pharmacol. | volume = 67 | issue = 4 | pages = 1369–81 | year = 2005 | month = April | pmid = 15665253 | doi = 10.1124/mol.104.008193 | url = }}</ref><ref name="Yan_2005">{{cite journal | author = Yan L, Herrington J, Goldberg E, Dulski PM, Bugianesi RM, Slaughter RS, Banerjee P, Brochu RM, Priest BT, Kaczorowski GJ, Rudy B, Garcia ML | title = Stichodactyla helianthus peptide, a pharmacological tool for studying K<sub>v</sub>3.2 channels | journal = Mol. Pharmacol. | volume = 67 | issue = 5 | pages = 1513–21 | year = 2005 | month = May | pmid = 15709110 | doi = 10.1124/mol.105.011064 | url = }}</ref> The peptide binds to all four subunits in the K<sub>v</sub>1.3 tetramer through its interaction with the shallow vestibule at the outer entrance of the ion conduction pathway.<ref name="Kalman_1998"/><ref name="Rauer_1999"/><ref name="Lanigan_2002">{{cite journal | author = Lanigan MD, Kalman K, Lefievre Y, Pennington MW, Chandy KG, Norton RS | title = Mutating a critical lysine in ShK toxin alters its binding configuration in the pore-vestibule region of the voltage-gated potassium channel, K<sub>v</sub>1.3 | journal = Biochemistry | volume = 41 | issue = 40 | pages = 11963–71 | year = 2002 | month = October | pmid = 12356296| doi = 10.1021/bi026400b }}</ref> The peptide's Lysine<sup>22</sup> residue occludes the channel pore like a "cork in a bottle". This blocks the entrance to the pore.<ref name="Chandy_2004">{{cite journal | author = Chandy KG, Wulff H, Beeton C, Pennington M, Gutman GA, Cahalan MD | title = K<sup>+</sup> channels as targets for specific immunomodulation | journal = Trends Pharmacol. Sci. | volume = 25 | issue = 5 | pages = 280–9 | year = 2004 | month = May | pmid = 15120495 | doi = 10.1016/j.tips.2004.03.010 | url = | pmc = 2749963 }}</ref><ref name="Norton_2004">{{cite journal | author = Norton RS, Pennington MW, Wulff H | title = Potassium channel blockade by the sea anemone toxin ShK for the treatment of multiple sclerosis and othfer autoimmune diseases | journal = Curr. Med. Chem. | volume = 11 | issue = 23 | pages = 3041–52 | year = 2004 | month = December | pmid = 15578998 | doi = | url = http://www.bentham-direct.org/pages/content.php?CMC/2004/00000011/00000023/0003C.SGM | issn = }}</ref>


ShK blocks the K<sub>v</sub>1.3 channel in T cells with a K<sub>d</sub> of about 11 pM.<ref name="Kalman_1998"/><ref name="Rauer_1999"/><ref name="Beeton_2003">{{cite journal | author = Beeton C, Wulff H, Singh S, Botsko S, Crossley G, Gutman GA, Cahalan MD, Pennington M, Chandy KG | title = A novel fluorescent toxin to detect and investigate K<sub>v</sub>1.3 channel up-regulation in chronically activated T lymphocytes | journal = J. Biol. Chem. | volume = 278 | issue = 11 | pages = 9928–37 | year = 2003 | month = March | pmid = 12511563 | doi = 10.1074/jbc.M212868200 | url = }}</ref> It blocks the neuronal K<sub>v</sub>1.1 and K<sub>v</sub>1.6 channels with K<sub>d</sub>s of 16 pM and 200 pM respectively.<ref name="Beeton_2005"/> The K<sub>v</sub>3.2 and K<sub>Ca</sub>3.1 channels are more than 1000 times less sensitive to the peptide.<ref name="Kalman_1998"/><ref name="Rauer_1999"/><ref name="Beeton_2005"/><ref name="Yan_2005"/>
ShK blocks the K<sub>v</sub>1.3 channel in T cells with a K<sub>d</sub> of about 11 pM.<ref name="Kalman_1998"/><ref name="Rauer_1999"/><ref name="Beeton_2003">{{cite journal | author = Beeton C, Wulff H, Singh S, Botsko S, Crossley G, Gutman GA, Cahalan MD, Pennington M, Chandy KG | title = A novel fluorescent toxin to detect and investigate K<sub>v</sub>1.3 channel up-regulation in chronically activated T lymphocytes | journal = J. Biol. Chem. | volume = 278 | issue = 11 | pages = 9928–37 | year = 2003 | month = March | pmid = 12511563 | doi = 10.1074/jbc.M212868200 | url = }}</ref> It blocks the neuronal K<sub>v</sub>1.1 and K<sub>v</sub>1.6 channels with K<sub>d</sub>s of 16 pM and 200 pM respectively.<ref name="Beeton_2005"/> The K<sub>v</sub>3.2 and K<sub>Ca</sub>3.1 channels are more than 1000 times less sensitive to the peptide.<ref name="Kalman_1998"/><ref name="Rauer_1999"/><ref name="Beeton_2005"/><ref name="Yan_2005"/>

Revision as of 15:09, 9 November 2012

ShK domain-like
Rainbow colored cartoon diagram (N-terminus = blue, C-terminus = red) of an NMR solution structure of the ShK toxin.[1] Sidechains of cysteine residues involved in disulfide linkages are displayed as sticks and the sulfur atoms in these links are colored yellow.
Identifiers
SymbolShK
PfamPF01549
InterProIPR003582
SMARTSM00254
SCOP21roo / SCOPe / SUPFAM
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary
PDB1bei​, 1bgk​, 1c2u​, 1roo​, 1rc9

Stichodactyla toxin (ShK) is a peptide toxin that blocks the voltage-gated potassium channels: Kv1.1, Kv1.3, Kv1.6, Kv3.2 and KCa3.1.

Structure

ShK is a 35-residue basic peptide first discovered in the sea anemone Stichodactyla helianthus by Professor Olga Castaneda from the University of Havana, Cuba, and her collaborators in Sweden. The formula is C169H274N54O48S7. [2] It is cross-linked by three disulfide bridges: Cys3-Cys35, Cys12-Cys28, and Cys17-Cys32 (see figure below).[3][4] The amino acid sequence of the ShK toxin is Arg-Ser-Cys-Ile-Asp-Thr-Ile-Pro-Lys-Ser-Arg-Cys-Thr-Ala-Phe-Gln-Cys-Lys-His-Ser-Met-Lys-Tyr-Arg-Leu-Ser-Phe-Cys-Arg-Lys-Thr-Cys-Gly-Thr-Cys.[2] ShK is stabilized by three disulfide bridges and consists of two short α-helices comprising residues 14-19 and 21-24.[1] The N-terminal eight residues of ShK adopt an extended conformation, followed by a pair of interlocking turns that resemble a 310 helix, while its C-terminal Cys35 residue forms a nearly head-to-tail cyclic structure through a disulfide bond with Cys3.[5] Protein domains with structural resemblance to ShK have been described in 402 proteins, most of them from C. elegans (InterProIPR003582). Other proteins containing domains with similar structures include the cysteine-rich secretory protein snake toxins natrin, triflin, and stecrisp, the Toxocara canis mucins and the human protein Tpx-1.[6][7][8][9][10]

Schematic diagram of the primary structure of the ShK peptide highlighting the three disulfide (–S–S–) linkages.

Target

ShK toxin blocks the K+ channels Kv1.1, Kv1.3, Kv1.6, Kv3.2 and KCa<3.1,[11][12][13][14][15] The peptide binds to all four subunits in the Kv1.3 tetramer through its interaction with the shallow vestibule at the outer entrance of the ion conduction pathway.[11][12][16] The peptide's Lysine22 residue occludes the channel pore like a "cork in a bottle". This blocks the entrance to the pore.[17][18]

ShK blocks the Kv1.3 channel in T cells with a Kd of about 11 pM.[11][12][19] It blocks the neuronal Kv1.1 and Kv1.6 channels with Kds of 16 pM and 200 pM respectively.[14] The Kv3.2 and KCa3.1 channels are more than 1000 times less sensitive to the peptide.[11][12][14][15]

Several ShK analogs have been generated to enhance specificity for the Kv1.3 channel over the Kv1.1, Kv1.6 and Kv3.2 channels. The first analog that showed some degree of specificity was ShK-Dap22.[11] Attaching a fluorescein to the N-terminus of the peptide via a hydrophilic AEEA linker (2-aminoethoxy-2-ethoxy acetic acid; mini-PEG) resulted in a peptide, ShK-F6CA, with 100-fold specificity for Kv1.3 over Kv1.1 and related channels.[19] Based on this surprising finding additional analogs were made. ShK-170 [a.k.a. ShK(L5)],contains a L-phosphotyrosine in place of the fluorescein in ShK-F6CA. It blocks Kv1.3 with a Kd of 69 pM and shows exquisite specificity for Kv1.3.[14] However, it is chemically unstable. To improve stability a new analog, ShK-186 [a.k.a. SL5], was made with the C-terminal carboxyl of ShK-170 replaced by an amide; ShK-186 is otherwise identical to ShK-170.[20][21] However, ShK-186 is also chemically unstable.[21] ShK-192 is a new analog with increased stability. It contains norleucine21 in place of methionine21 to avoid methionine oxidation, and the terminal phosphotyrosine is replaced by a non-hydrolyzable para-phosphonophenylalanine (Ppa) group.[21] The D-diasteromer of ShK is also stable but blocks Kv1.3 with 2800-fold potency than the L-form (Kd = 36 nM) and it only exhibits 2-fold specificity for Kv1.3 over Kv1.1.[22]

Kv1.3 and KCa3.1 regulate membrane potential and calcium signaling of T cells.[17] Calcium entry through the CRAC channel is promoted by potassium efflux through the Kv1.3 and KCa3.1 potassium channels.[20] Blockade of Kv1.3 channels in effector-memory T cells by ShK-186 suppresses calcium signaling, cytokine production (interferon-gamma, interleukin 2) and cell proliferation.[17][23][20] In vivo, ShK-186 paralyzes effector-memory T cells at the sites of inflammation and prevent their reactivation in inflamed tissues.[24] In contrast, ShK-186 does not affect the homing to and motility within lymph nodes of naive and central memory T cells, most likely because these cells express the KCa3.1 channel and are therefore protected from the effect of Kv1.3 blockade.[24] In proof-of-concept studies, ShK and its analogs have prevented and treated disease in rat models of multiple sclerosis, rheumatoid arthritis, and delayed type hypersensitivity.[19][25][14][25]

As ShK toxin binds to the synaptosomal membranes, it facilitates an acetylcholine release at avian neuromuscular junctions while the Kv3.2 channels are expressed in neurons that fire at a high frequency (such as cortical GABAergic interneurons), due to their fast activation and deactivation rates.[15] By blocking Kv3.2, ShK toxin depolarises the cortical GABAergic interneurons. Kv3.2 is also expressed in pancreatic beta cells. These cells are thought to play a role in their delayed-rectifier current, which regulates glucose-dependent firing. Therefore, ShK, as a Kv3.2 blocker, might be useful in the treatment of type-2 diabetes, although inhibition of the delayed-rectifier current has not yet been observed in human cells even when very high ShK concentrations were used.[15]

Toxicity

Toxicity of ShK toxin in mice is quite low. The median paralytic dose is about 25 mg/kg bodyweight (which translates to 0.5 mg per 20 g mouse). In rats the therapeutic safety index was greater than 75-fold.

ShK-Dap22 is less toxic, even a dose of 1.0 mg dose did not cause hyperactivity, seizures or mortality. The median paralytic dose was 200 mg/kg body weight.[11]

ShK-170 [a.k.a. ShK(L5)] does not cause significant toxicity in vitro. The peptide was not toxic to human and rat lymphoid cells incubated for 48 h with 100 nM of ShK-170 (>1200 times greater than the Kv1.3 half-blocking dose). The same high concentration of ShK-170 was negative in the Ames test on tester strain TA97A, suggesting that it is not a mutagen. ShK-170 had no effect on heart rate or heart rate variability parameters in either the time or the frequency domain in rats. It does not block the hERG (Kv11.1) channel that is associated with drug-associated cardiac arrhythmias. Repeated daily administration of the peptide by subcutaneous injection (10 µg/kg/day) for 2 weeks to rats does not cause any changes in blood counts, blood chemistry or in the proportion of thymocyte or lymphocyte subsets. Furthermore, the rats administered the peptide gain weight normally.

ShK-186 [a.k.a. SL5] is also safe. Repeated daily administration by subcutaneous injection of ShK-186 (100 µg/kg/day) for 4 weeks to rats does not cause any changes in blood counts, blood chemistry or histopathology.[20] Furthermore, ShK-186 did not compromise the protective immune response to acute influenza viral infection or acute bacterial (Chlamydia) infection at concentrations that were effective in ameliorating autoimmune diseases in rat models.[24] Interestingly, rats repeatedly administered ShK-186 for a month by subcutaneous injection (500 µg/kg/day) did not develop anti-ShK antibodies.[25] The reason for the low immunogenicity of the peptide is not well understood.

Many groups are developing Kv1.3 blockers for the treatment of autoimmune diseases.[26]

Use

Because ShK toxin is a specific inhibitor of Kv1.1, Kv1.3, Kv1.6, Kv3.2 and KCa3.1, it may serve as a useful pharmacological tool for studying these channels.[15][19] The Kv1.3 specific ShK analogs, ShK-170, ShK-186 and ShK-192, have been demonstrated to be effective in rat models of autoimmune diseases, and these or related analogs might have use as therapeutics for human autoimmune diseases.

Kv1.3 is also considered a therapeutic target for the treatment of obesity,[27][28] for enhancing peripheral insulin sensitivity in patients with type-2 diabetes mellitus,[29] and for preventing bone resorption in periodontal disease.[30] Furthermore, because pancreatic beta cells, which have Kv3.2 channels, are thought to play a role in glucose-dependent firing, ShK, as a Kv3.2 blocker, might be useful in the treatment of type-2 diabetes, although inhibition of the delayed-rectifier current has not yet been observed in human cells even when very high ShK concentrations were used.[14]

References

  1. ^ a b PDB: 1ROO​; Tudor JE, Pallaghy PK, Pennington MW, Norton RS (1996). "Solution structure of ShK toxin, a novel potassium channel inhibitor from a sea anemone". Nat. Struct. Biol. 3 (4): 317–20. doi:10.1038/nsb0496-317. PMID 8599755. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  2. ^ a b Castañeda O, Sotolongo V, Amor AM, Stöcklin R, Anderson AJ, Harvey AL, Engström A, Wernstedt C, Karlsson E (1995). "Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus". Toxicon. 33 (5): 603–13. doi:10.1016/0041-0101(95)00013-C. PMID 7660365. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  3. ^ Pennington MW, Mahnir VM, Khaytin I, Zaydenberg I, Byrnes ME, Kem WR (1996). "An essential binding surface for ShK toxin interaction with rat brain potassium channels". Biochemistry. 35 (51): 16407–11. doi:10.1021/bi962463g. PMID 8987971. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  4. ^ Pennington MW, Lanigan MD, Kalman K, Mahnir VM, Rauer H, McVaugh CT, Behm D, Donaldson D, Chandy KG, Kem WR, Norton RS (1999). "Role of disulfide bonds in the structure and potassium channel blocking activity of ShK toxin". Biochemistry. 38 (44): 14549–58. doi:10.1021/bi991282m. PMID 10545177. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  5. ^ Pohl J, Hubalek F, Byrnes ME, Nielsen KR, Woods A and Pennington MW (1995). "Assignment of the three disulfide bonds in ShK toxin: A potent potassium channel inhibitor from the sea anemone Stichodactyla helianthus" (PDF). Letters in Peptide Science. 1 (6): 291–297. doi:10.1007/BF00119770.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  6. ^ Wang F, Li H, Liu MN, Song H, Han HM, Wang QL, Yin CC, Zhou YC, Qi Z, Shu YY, Lin ZJ, Jiang T (2006). "Structural and functional analysis of natrin, a venom protein that targets various ion channels". Biochem. Biophys. Res. Commun. 351 (2): 443–8. doi:10.1016/j.bbrc.2006.10.067. PMID 17070778. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  7. ^ Shikamoto Y, Suto K, Yamazaki Y, Morita T, Mizuno H (2005). "Crystal structure of a CRISP family Ca2+ -channel blocker derived from snake venom". J. Mol. Biol. 350 (4): 735–43. doi:10.1016/j.jmb.2005.05.020. PMID 15953617. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  8. ^ Guo M, Teng M, Niu L, Liu Q, Huang Q, Hao Q (2005). "Crystal structure of the cysteine-rich secretory protein stecrisp reveals that the cysteine-rich domain has a K+ channel inhibitor-like fold". J. Biol. Chem. 280 (13): 12405–12. doi:10.1074/jbc.M413566200. PMID 15596436. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  9. ^ Gibbs GM, Scanlon MJ, Swarbrick J, Curtis S, Gallant E, Dulhunty AF, O'Bryan MK (2006). "The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling". J. Biol. Chem. 281 (7): 4156–63. doi:10.1074/jbc.M506849200. PMID 16339766. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  10. ^ Loukas A, Hintz M, Linder D, Mullin NP, Parkinson J, Tetteh KK, Maizels RM (2000). "A family of secreted mucins from the parasitic nematode Toxocara canis bears diverse mucin domains but shares similar flanking six-cysteine repeat motifs". J. Biol. Chem. 275 (50): 39600–7. doi:10.1074/jbc.M005632200. PMID 10950959. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  11. ^ a b c d e f Kalman K, Pennington MW, Lanigan MD, Nguyen A, Rauer H, Mahnir V, Paschetto K, Kem WR, Grissmer S, Gutman GA, Christian EP, Cahalan MD, Norton RS, Chandy KG (1998). "ShK-Dap22, a potent Kv1.3-specific immunosuppressive polypeptide". J. Biol. Chem. 273 (49): 32697–707. doi:10.1074/jbc.273.49.32697. PMID 9830012. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  12. ^ a b c d Rauer H, Pennington M, Cahalan M, Chandy KG (1999). "Structural conservation of the pores of calcium-activated and voltage-gated potassium channels determined by a sea anemone toxin". J. Biol. Chem. 274 (31): 21885–92. doi:10.1074/jbc.274.31.21885. PMID 10419508. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  13. ^ Middleton RE, Sanchez M, Linde AR, Bugianesi RM, Dai G, Felix JP, Koprak SL, Staruch MJ, Bruguera M, Cox R, Ghosh A, Hwang J, Jones S, Kohler M, Slaughter RS, McManus OB, Kaczorowski GJ, Garcia ML (2003). "Substitution of a single residue in Stichodactyla helianthus peptide, ShK-Dap22, reveals a novel pharmacological profile". Biochemistry. 42 (46): 13698–707. doi:10.1021/bi035209e. PMID 14622016. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  14. ^ a b c d e f Beeton C, Pennington MW, Wulff H, Singh S, Nugent D, Crossley G, Khaytin I, Calabresi PA, Chen CY, Gutman GA, Chandy KG (2005). "Targeting effector memory T cells with a selective peptide inhibitor of Kv1.3 channels for therapy of autoimmune diseases". Mol. Pharmacol. 67 (4): 1369–81. doi:10.1124/mol.104.008193. PMID 15665253. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  15. ^ a b c d e Yan L, Herrington J, Goldberg E, Dulski PM, Bugianesi RM, Slaughter RS, Banerjee P, Brochu RM, Priest BT, Kaczorowski GJ, Rudy B, Garcia ML (2005). "Stichodactyla helianthus peptide, a pharmacological tool for studying Kv3.2 channels". Mol. Pharmacol. 67 (5): 1513–21. doi:10.1124/mol.105.011064. PMID 15709110. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  16. ^ Lanigan MD, Kalman K, Lefievre Y, Pennington MW, Chandy KG, Norton RS (2002). "Mutating a critical lysine in ShK toxin alters its binding configuration in the pore-vestibule region of the voltage-gated potassium channel, Kv1.3". Biochemistry. 41 (40): 11963–71. doi:10.1021/bi026400b. PMID 12356296. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  17. ^ a b c Chandy KG, Wulff H, Beeton C, Pennington M, Gutman GA, Cahalan MD (2004). "K+ channels as targets for specific immunomodulation". Trends Pharmacol. Sci. 25 (5): 280–9. doi:10.1016/j.tips.2004.03.010. PMC 2749963. PMID 15120495. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  18. ^ Norton RS, Pennington MW, Wulff H (2004). "Potassium channel blockade by the sea anemone toxin ShK for the treatment of multiple sclerosis and othfer autoimmune diseases". Curr. Med. Chem. 11 (23): 3041–52. PMID 15578998. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  19. ^ a b c d Beeton C, Wulff H, Singh S, Botsko S, Crossley G, Gutman GA, Cahalan MD, Pennington M, Chandy KG (2003). "A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes". J. Biol. Chem. 278 (11): 9928–37. doi:10.1074/jbc.M212868200. PMID 12511563. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  20. ^ a b c d Beeton C, Wulff H, Standifer NE, Azam P, Mullen KM, Pennington MW, Kolski-Andreaco A, Wei E, Grino A, Counts DR, Wang PH, LeeHealey CJ, S Andrews B, Sankaranarayanan A, Homerick D, Roeck WW, Tehranzadeh J, Stanhope KL, Zimin P, Havel PJ, Griffey S, Knaus HG, Nepom GT, Gutman GA, Calabresi PA, Chandy KG (2006). "Kv1.3 channels are a therapeutic target for T cell-mediated autoimmune diseases". Proc. Natl. Acad. Sci. U.S.A. 103 (46): 17414–9. doi:10.1073/pnas.0605136103. PMC 1859943. PMID 17088564. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  21. ^ a b c Pennington MW, Beeton C, Galea CA, Smith BJ, Chi V, Monaghan KP, Garcia A, Rangaraju S, Giuffrida A, Plank D, Crossley G, Nugent D, Khaytin I, Lefievre Y, Peshenko I, Dixon C, Chauhan S, Orzel A, Inoue T, Hu X, Moore RV, Norton RS, Chandy KG (2009). "Engineering a stable and selective peptide blocker of the Kv1.3 channel in T lymphocytes". Mol. Pharmacol. 75 (4): 762–73. doi:10.1124/mol.108.052704. PMC 2684922. PMID 19122005. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  22. ^ Beeton C, Smith BJ, Sabo JK, Crossley G, Nugent D, Khaytin I, Chi V, Chandy KG, Pennington MW, Norton RS (2008). "The D-diastereomer of ShK toxin selectively blocks voltage-gated K+ channels and inhibits T lymphocyte proliferation". J. Biol. Chem. 283 (2): 988–97. doi:10.1074/jbc.M706008200. PMID 17984097. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  23. ^ Wulff H, Calabresi PA, Allie R, Yun S, Pennington M, Beeton C, Chandy KG (2003). "The voltage-gated Kv1.3 K+ channel in effector memory T cells as new target for MS". J. Clin. Invest. 111 (11): 1703–13. doi:10.1172/JCI16921. PMC 156104. PMID 12782673. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  24. ^ a b c Matheu MP, Beeton C, Garcia A, Chi V, Rangaraju S, Safrina O, Monaghan K, Uemura MI, Li D, Pal S, de la Maza LM, Monuki E, Flügel A, Pennington MW, Parker I, Chandy KG, Cahalan MD (2008). "Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block". Immunity. 29 (4): 602–14. doi:10.1016/j.immuni.2008.07.015. PMC 2732399. PMID 18835197. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  25. ^ a b c Beeton C, Wulff H, Barbaria J, Clot-Faybesse O, Pennington M, Bernard D, Cahalan MD, Chandy KG, Béraud E (2001). "Selective blockade of T lymphocyte K+ channels ameliorates experimental autoimmune encephalomyelitis, a model for multiple sclerosis". Proc. Natl. Acad. Sci. U.S.A. 98 (24): 13942–7. doi:10.1073/pnas.241497298. PMC 61146. PMID 11717451. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  26. ^ Wulff H, Beeton C, Chandy KG (2003). "Potassium channels as therapeutic targets for autoimmune disorders". Curr Opin Drug Discov Devel. 6 (5): 640–7. PMID 14579513. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  27. ^ Tucker K, Overton JM, Fadool DA (2008). "Kv1.3 gene-targeted deletion alters longevity and reduces adiposity by increasing locomotion and metabolism in melanocortin-4 receptor-null mice". Int J Obes (Lond). 32 (8): 1222–32. doi:10.1038/ijo.2008.77. PMC 2737548. PMID 18542083. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  28. ^ Xu J, Koni PA, Wang P, Li G, Kaczmarek L, Wu Y, Li Y, Flavell RA, Desir GV (2003). "The voltage-gated potassium channel Kv1.3 regulates energy homeostasis and body weight". Hum. Mol. Genet. 12 (5): 551–9. doi:10.1093/hmg/ddg049. PMID 12588802. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  29. ^ Xu J, Wang P, Li Y, Li G, Kaczmarek LK, Wu Y, Koni PA, Flavell RA, Desir GV (2004). "The voltage-gated potassium channel Kv1.3 regulates peripheral insulin sensitivity". Proc. Natl. Acad. Sci. U.S.A. 101 (9): 3112–7. doi:10.1073/pnas.0308450100. PMC 365752. PMID 14981264. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  30. ^ Valverde P, Kawai T, Taubman MA (2005). "Potassium channel-blockers as therapeutic agents to interfere with bone resorption of periodontal disease". J. Dent. Res. 84 (6): 488–99. doi:10.1177/154405910508400603. PMID 15914584. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)