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Another recent study from The Journal of Alternative and Complementary Medicine found that grapefruit-seed extract was effective in killing over "800 bacterial and viral strains, 100 strains of fungus, and a large number of single and multi-celled parasites."² No other naturally-occuring anti-microbial can come close to these results.
Another recent study from The Journal of Alternative and Complementary Medicine found that grapefruit-seed extract was effective in killing over "800 bacterial and viral strains, 100 strains of fungus, and a large number of single and multi-celled parasites."² No other naturally-occuring anti-microbial can come close to these results.

== References ==
{{reflist}}


== External links ==
== External links ==

Revision as of 15:52, 13 November 2012

Grapefruit seed extract (GSE), also known as citrus seed extract, is a liquid derived from the seeds, pulp, and white membranes of grapefruit.[1] Self-made natural GSE processed in the laboratory without solvents or synthetic agents is prepared by grinding the grapefruit seed and juiceless pulp, then mixing with glycerin.[1] Commercially available GSE sold to consumers are made from the seed, pulp, glycerin, and synthetic preservatives all blended together.[1] Grapefruit seed extract is sold as a food supplement and used in cosmetics because it is a claimed natural antimicrobial.[2]

An early proponent was Dr. Jacob Harich (1919–1996).[3] A long time promoter of GSE was Aubrey Hampton, the founder of Aubrey Organics.[4] Some marketers of GSE affirm this extract to be a safe, natural, and an effective preservative.[5] This extract has been stated by some practitioners of alternative medicine to possess antibacterial, antiviral, and antifungal properties.[3] It has been recommended by some nutritionists for the treatment of candidiasis, earache, throat infections, and diarrhea.[3] GSE is promoted at health food shops and on the internet.[6][7]

There are conflicting scientific demonstrations of the efficacy of GSE.[1] Suspicions about the true nature of the active compounds in GSEs arose when synthetic additives were found in commercial products.[2] Suspected counterfeit grapefruit seed extracts were adulterated with synthetic preservatives.[8][9][10] When preservatives were not present in some of the extracts, laboratory tests found the natural extracts had no natural antimicrobial attributes of their own.[5] Some manufacturers of GSE have stated that their extract has compounds nearly identical to benzethonium chloride, but a 2001 study supervised by chemist G. Takeoka has documented that commercial GSE preparations contain the synthetic compound benzethonium chloride that could not have been made from GSE.[5]

History

The grapefruit is a subtropical citrus tree grown for its fruit which was originally named the "forbidden fruit" of Barbados.[11] The fruit was first documented in 1750 by Rev. Griffith Hughes describing specimens from Barbados.[12] All parts of the fruit have uses. The fruit is mainly consumed for a tangy juice.[13] The peel is expressed into an aromatherapy oil[14] and is also a source of dietary fiber.[15] The seed and pulp as a by-product of the juice industry is sold as cattle feed[16] and is manufactured for use to make an extract.[1] GSE was originally developed by Dr. Jacob Harich, a nuclear physicist. In 1963, he journeyed to Florida, the heart of grapefruit country in the U.S., and began researching and later marketing of GSE.[3] Aubrey Hampton, founder of Aubrey Organics, has promoted citrus seed extract, a component in "Aubrey’s Preservative", for more than 25 years.[4] Today, GSE is mostly promoted at health food shops and on the internet.[6][7]

Efficacy

Anecdotal claims for an antimicrobial effect are based on a few abstract scientific experiments which show some antimicrobial activity for commercial grapefruit seed extracts and an ethanol grapefruit seed extract but not a natural GSE.[17][18][19] The preliminary studies for the antimicrobial effect state that the Citricidal commercial brand of GSE was used for testing.[17][18] There is considerable scientific evidence that the anti-microbial activity associated with grapefruit seed extract is attributable to the contamination or adulteration of commercial GSE preparations with synthetic antimicrobials or preservatives.[2] According to the 2001 GSE study, branded commercial GSE preparations were contaminated with synthetic benzethonium chloride that was implausible to be made from grapefruit seeds.[5] Some of the marketers claimed that their product does not contain benzethonium chloride and the error was due to the similarity in molecular weight of the quaternary ammonium compound which was formed through a proprietary manufacturing process.[5] It is inconceivable that the preservative benzethonium chloride is formed during any extraction and/or processing of grapefruit seeds and pulp.[5] Researchers conclusively demonstrated the presence of benzethonium chloride in commercial GSE using various analytical methods including HPLC, one- and two-dimensional NMR, PIXE analysis, and electrospray ionization MS.[5] Another in vitro study claimed GSE had antibacterial properties.[19] As with the branded in vitro test[17][18] this extract was contaminated.[5] The ethanolic grapefruit seed and pulp extract tested was prepared or contaminated with ethanol, a chemical solvent used in the extraction process.[19] Ethanol, also known as ethyl alcohol, is effective in inhibiting bacteria.[20]

Independent studies have shown that commercial preparations contain the compound benzalkonium chloride, which is a synthetic antimicrobial commonly used in disinfectants and cleaning products, the related compound benzethonium chloride, the antibiotic triclosan, or the preservative methylparaben.[1][6][21] Some samples were shown to contain up to 22% benzalkonium chloride by weight, despite the known allergenicity[22] and toxicity[23] of the compound at higher doses.[6] These chemicals were not present in grapefruit seed extracts prepared in the laboratory, and GSE preparations without the contaminants were found to possess no detectable antimicrobial effect.[1] Although citrus seed extract is sold in health food markets,[6] there is no good evidence for any natural antimicrobial activity.[1][7]

A study that examined the antiviral properties of GSE found that GSE had no efficacy as a disinfectant for feline calicivirus and feline parvovirus.[24] Grapefruit seed extract has been advocated to be a powerful antimicrobial with proven activity against bacteria and fungi. However, independent studies have shown the efficacy of grapefruit seed extract as an antimicrobial is not demonstrated. Although citrus seed extract is claimed to be a highly effective plant-based preservative by some natural personal care manufacturers, studies asserted that the universal antimicrobial benefits associated with GSE are merely from the presence of synthetic preservatives. Natural GSE has no antimicrobial properties.[1][2] Newer studies found many commercial grapefruit seed extracts contaminated with synthetic preservatives.[8][9][10] In a 2007 study the main synthetic antimicrobial agent identified in commercial GSE samples was benzethonium chloride.[8] In a 2008 study synthetic disinfectants such as benzethonium or benzalkonium chloride were identified in most of the commercial GSE products.[9] In a 2008 study suspected counterfeit products were determined to contain benzethonium chloride in grapefruit seed extract samples.[10]

Methodology

Grapefruit seed extracts are added to cosmetics, food supplements, and pesticides for their antimicrobial effect, but suspicions about the true nature of the active compounds in GSEs arose when synthetic additives were found in commercial products.[2] Various analytical methods were developed to determine the constituents or compounds in GSE samples which include particle-induced x-ray emission (PIXE),[5] electrospray ionization mass spectrometry (ESI-MS),[5] high performance liquid chromatography (HPLC),[2] liquid chromatography-mass spectrometry (LC-MS),[9] and nuclear magnetic resonance (NMR) analysis.[10]

Properties

There is evidence that the extract of citrus grapefruit seed has antioxidant properties.[25][26] Analysis shows the constituents of the seed extract and pulp are flavonoids,[27][28] ascorbic acid (commonly known as vitamin C[29][30][31]), tocopherols, citric acid,[25] limonoids,[32][33][34] sterols, and minerals.[35] A study suggests the consumption of the bioactive compounds found in grapefruit seed and pulp may help suppress the development of colon cancer.[36] Another study showed the flavonoid naringin is an excellent plasma lipid lowering and plasma antioxidant active elevating flavonone.[37]

Preparations

Testing was conducted by the U. of Georgia, in Athens, GA, to evaluate grapefruit seed extractin tests against E. Coli, Salmonella sps., and Staph aureus. Roger Wyatt, Ph.D., and Microbiologist for the U. of GA, reported,

"Our studies indicate excellent potential for these products(GSE). ...The toxicological that I have reviewed indicated that this product and the active ingredient poses very low toxicity. As you know this is important because most disinfectants that are currently used in either animal or human environments have moderate to high toxicity and extreme care must be exercised when these products are used. The lack of any significant toxicological properties of (GSE) is also impressive when one views the efficacy data where extemely small concentrations of the product can be used with markedbeneficial results." Dr. Wyatt continued, "In view of the reports that we have discussed, the wide spectrum of activity that (GSE) offers(antiviral, antibacterial; Gram+ and Gram-, antimycotic, and antiprotozoan) will undoubtedly aid in its acceptability."

Dr. Wyatt's findings have also been confirmed by a variety of clinics and labs, as well as universities from around the world, including the University of Arkansas, the U. of Malaya, the U. of Sao Paulo, and the University Ricardo Palma, to name a few.

Precautions

Grapefruit can have a number of interactions with certain drugs, increasing the potency of many compounds,[38] including warfarin (coumadin).[39] Grapefruit has components that inhibit the CYP3A4 enzyme in the intestine.[38] Thus, it is this effect that increases the rate of absorption of several drugs.[40] There are concerns about the safety of ingesting synthetic preservatives found in grapefruit seed extract.[7]

Controversy

GSE usage has been popularized by many companies within the scope of the health food industry.[5] There are books exclusively dedicated to GSE.[3][41][42] This extract is promoted by marketers on the internet, there are health food stores that recommend its use, and proponents maintain its safe.[5][7] Some consumers believe this extract is an effective natural preservative even though multiple scientific studies have concluded that the universal antimicrobial activity is merely from contamination with synthetic antimicrobials.[1][2][5][6][21][39]

GSE is marketed as an all-natural and perfectly safe health food product, when it is neither. Various Citrus species have been used as a food and medicine in Chinese and Ayurvedic medicine for thousands of years. While they have many many benefits, neither Chinese nor Ayurvedic medicine has ever advocated the use of Grapefruit seed or any other Citrus seed in acute infectious disease: this alone should provide some clue as to its effects or lack thereof.

— Todd Caldecott, [7]


Grapefruit Seed Extract (GSE) has very high amounts of disease-fighting, free-radical eliminating antioxidants and phytonutrients called bioflavonoids. One of these powerful bioflavanoids (plant antioxidants) include the chemical component hesperidin, a well-known natural immune-system stimulator and booster.4


Chemical analysis of grapefruit seed extract shows additional antioxidant constituents including Vitamin C, sterols, tocopherols, citric acid, liminoids, and other trace minerals. Perhaps most exciting in regard to GSE's health benefits is its anti-cancer potentials. Studies done on the compounds in GSE's seed and pulp have found that these bioactive agents may be able to inhibit the occurrence of colon cancer. 6

A recent study done by Microbiologists from the University of Georgia found that GSE was a very effective non-toxic disinfectant. Sadly, commonly-used disinfectant have high levels of toxicity and are harmful to humans, animals and the environment. The same study found that GSE demonstrates a "wide spectrum of activity," including antiviral, antimycotic, and antiprotozoan capacities against many bacteria and viruses, including E.Coli.¹ It was also found to be an effective cleanser as a a pre-surgical soap.¹

Another recent study from The Journal of Alternative and Complementary Medicine found that grapefruit-seed extract was effective in killing over "800 bacterial and viral strains, 100 strains of fungus, and a large number of single and multi-celled parasites."² No other naturally-occuring anti-microbial can come close to these results.

  1. ^ a b c d e f g h i j von Woedtke T, Schlüter B, Pflegel P, Lindequist U, Jülich WD (1999). "Aspects of the antimicrobial efficacy of grapefruit seed extract and its relation to preservative substances contained". Pharmazie. 54 (6): 452–6. PMID 10399191. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  2. ^ a b c d e f g Ganzera M, Aberham A, Stuppner H (2006). "Development and validation of an HPLC/UV/MS method for simultaneous determination of 18 preservatives in grapefruit seed extract". J. Agric. Food Chem. 54 (11): 3768–72. doi:10.1021/jf060543d. PMID 16719494. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  3. ^ a b c d e Sims, Judith. Gale Encyclopedia of Alternative Medicine. Available in e-book format. Thomson Gale. 2001.
  4. ^ a b Melissa Meisel. Cosmetics Go Green. Happi, Rodman Publishing, June 2007.
  5. ^ a b c d e f g h i j k l m Takeoka G, Dao L, Wong RY, Lundin R, Mahoney N (2001). "Identification of benzethonium chloride in commercial grapefruit seed extracts". J. Agric. Food Chem. 49 (7): 3316–20. doi:10.1021/jf010222w. PMID 11453769. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  6. ^ a b c d e f Takeoka GR, Dao LT, Wong RY, Harden LA (2005). "Identification of benzalkonium chloride in commercial grapefruit seed extracts". J. Agric. Food Chem. 53 (19): 7630–6. doi:10.1021/jf0514064. PMID 16159196. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
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  29. ^ Andrew W. Saul, Ph.D. Vitamin C Has Been Known To Fight 30 Major Diseases... For Over 50 Years. Orthomolecular Medicine News Service. March 15, 2006.
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