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==Applications==
==Applications==
Time-lapse microscopy can be used to observe any microscopic object over time.
Time-lapse microscopy can be used to observe any microscopic object over time.
However, its main use is within [[cell biology]] to observe artificially [[cell culture|cultured cells]].
However, its main use is within [[cell biology]] to observe artificially [[cell culture|cultured cells]]. Depending on the cell culture, different microscopy techniques can be applied to enhance characteristics of the cells as most cells are transparent.<ref name="NobelPhase">{{cite web
Most cells are completely transparent. This makes it difficult to observe living cells in a light microscope.
To allow observation, cells has therefore traditionally been [[cell staining|stained]] before observation.
Unfortunately, the staining process kills the cells.<ref name="NobelPhase">{{cite web
| title=The Phase Contrast Microscope
| title=The Phase Contrast Microscope
| publisher=Nobel Media AB
| publisher=Nobel Media AB
| url=http://www.nobelprize.org/educational/physics/microscopes/phase}}</ref>
| url=http://www.nobelprize.org/educational/physics/microscopes/phase}}</ref>


The development of less destructive staining methods and methods to observe unstained cells has led to that
To enhance observations further, cells has therefore traditionally been [[cell staining|stained]] before observation. Unfortunately, the staining process kills the cells. The development of less destructive staining methods and methods to observe unstained cells has led to that cell biologists increasingly observe living cells. This is known as [[live cell imaging]].

cell biologists increasingly observe living cells. This is known as [[live cell imaging]].
Time-lapse microscopy is the method that extends live cell imaging from a single observation in time
Time-lapse microscopy is the method that extends live cell imaging from a single observation in time
to the observation of cellular dynamics over long periods of time.<ref name="Coutu">{{cite pmid|23943879}}</ref><ref>{{cite pmid|19953685}}</ref>
to the observation of cellular dynamics over long periods of time.<ref name="Coutu">{{cite pmid|23943879}}</ref><ref>{{cite pmid|19953685}}</ref>. Time-lapse microscopy is primarily used in research, but is clinically used [[IVF]] clinics as studies has proven it to increase pregnancy rates, lower abortion rates and predict [[aneuploidy]]<ref>http://www.fertstert.org/article/S0015-0282(12)02068-7/abstract</ref><ref>http://www.rbmojournal.com/article/S1472-6483(13)00070-9/abstract</ref>


Modern approaches are further extending time-lapse microscopy observations beyond making movies of cellular dynamics.
Modern approaches are further extending time-lapse microscopy observations beyond making movies of cellular dynamics.

Revision as of 20:20, 21 October 2013

Time-lapse microscope
A time-lapse microscope. Notice the transparent cell incubator which is necessary to keep cells alive during observation.
Other names(Time-lapse) microcinematograph, (Time-lapse) video microscope, Time-lapse cinemicrograph
UsesObservation of slow microscopic processes
InventorJean Comandon and other contemporaries
Related itemsTime-lapse photography, Live cell imaging
A time-lapse microscopy movie of dividing cancer cells.

Time-lapse microscopy is time-lapse photography applied to microscopy. Microscope image sequences are recorded and then viewed at a greater speed to give an accelerated view of the microscopic process.

Before the introduction of the video tape recorder in the 1960s, time-lapse microscopy recordings where made on photographic film. During this period, time-lapse microscopy was referred to as microcinematography. With the increasing use of video recorders, the term time-lapse video microscopy was gradually adopted. Today, the term video is increasingly dropped, reflecting that a digital still camera is used to record the individual image frames, instead of a video recorder.

Applications

Time-lapse microscopy can be used to observe any microscopic object over time. However, its main use is within cell biology to observe artificially cultured cells. Depending on the cell culture, different microscopy techniques can be applied to enhance characteristics of the cells as most cells are transparent.[1]

To enhance observations further, cells has therefore traditionally been stained before observation. Unfortunately, the staining process kills the cells. The development of less destructive staining methods and methods to observe unstained cells has led to that cell biologists increasingly observe living cells. This is known as live cell imaging.

Time-lapse microscopy is the method that extends live cell imaging from a single observation in time to the observation of cellular dynamics over long periods of time.[2][3]. Time-lapse microscopy is primarily used in research, but is clinically used IVF clinics as studies has proven it to increase pregnancy rates, lower abortion rates and predict aneuploidy[4][5]

Modern approaches are further extending time-lapse microscopy observations beyond making movies of cellular dynamics. Traditionally, cells have been observed in a microscope and measured in a cytometer. Increasingly this boundary is blurred as cytometric techniques are being integrated with imaging techniques for monitoring and measuring dynamic activities of cells and subcellular structures.[2][6][7]

Jean Comandon, pioneer of microcinematography, recorded this time-lapse film in c. 1910, using an ultramicroscope. The film show living spiral shaped syphilis bacteria moving among red blood cells of frog. Notice the back-and-forth movement, characterizing the disease-causing form.

History

One of the microcinematographs used at the Marey Institute during the late 19th century.

The Cheese Mites by Martin Duncan from 1903 is one of the earliest microcinematographic films.[8] However, the early development of scientific microcinematography took place in Paris. The first reported time-lapse microscope was assembled in the late 1890s at the Marey Institute, founded by the pioneer of chronophotography, Étienne-Jules Marey.[9][10][11] It was, however, Jean Comandon who made the first significant scientific contributions in around 1910.[12][13]

Comandon was a trained microbiologist specializing in syphilis research. Inspired by Victor Henri's microcinematic work on Brownian motion,[14][15][16] he used the newly invented ultramicroscope to study the movements of the syphilis bacteria.[17] At the time, the ultramicroscope was the only microscope in which the thin spiral shaped bacteria was visible. Using an enormous cinema camera bolted to the fragile microscope, he demonstrated visually that the movement of the disease-causing bacteria is uniquely different from the non-disease-causing form. Comandon’s films proved instrumental in teaching doctors how to distinguish the two forms.[18][19]

Comandon's extensive pioneering work inspired others to adopt microcinematography. Heniz Rosenberger builds a microcinematograph in the mid 1920s. In collerboration with Alexis Carrel, they used the device to further develop Carrel's cell culturing techniques.[20] Similar work was conducted by Warren Lewis.[21]

During the second World War II Carl Zeiss AG released the first phase contrast microscope on the market. With this new microscope, cellular details could for the first time be observed without using lethal stains.[1] By setting up some of the first time-lapse experiments with chicken fibroblasts and a phase contrast microscope, Michael Abercrombie described the basis of our current understanding of cell migration in 1953.[22][23]

With the broad introduction of the digital camera at the begnning of this century, time-lapse microscopy has been made dramatically more accessible and is currently experiencing an unrepresented raise in scientific publications.[2]

Historic time-lapse microscopy films

See also

References

  1. ^ a b "The Phase Contrast Microscope". Nobel Media AB.
  2. ^ a b c Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 23943879, please use {{cite journal}} with |pmid=23943879 instead.
  3. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 19953685, please use {{cite journal}} with |pmid=19953685 instead.
  4. ^ http://www.fertstert.org/article/S0015-0282(12)02068-7/abstract
  5. ^ http://www.rbmojournal.com/article/S1472-6483(13)00070-9/abstract
  6. ^ Mihaylova, Emilia, ed. (2013). "Chapter 6: Cells and Holograms – Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells". Holography - Basic Principles and Contemporary Applications. INTECH. doi:10.5772/54505. {{cite book}}: Unknown parameter |authors= ignored (help)
  7. ^ Egelberg, Peter. "Time-lape Image Cytometry". Phase Holographic Imaging AB.
  8. ^ Rohrer, Finlo. "Cheese mites and other wonders". BBC News Magazine. Retrieved 2011-04-24.
  9. ^ Talbot, Frederick A. (1913). Practical cinematography and its applications. W. Heinemann.
  10. ^ "Le cinéma au service de la science". Institut national de l'audiovisuel. Retrieved 2013-01-09.
  11. ^ Landecker, Hannah (2006). "Microcinematography and the History of Science and Film". Isis. 97. The University of Chicago Press on behalf of The History of Science Society: 121–132. doi:10.1086/501105.
  12. ^ "Jean Comandon (1877-1970)". Institut Pasteur.
  13. ^ "MICROBES CAUGHT IN ACTION.; Moving Pictures of Them a Great Aid In Medical Research". The New York Times. October 31, 1909.
  14. ^ Bigg, Charlotte (2011). "Chapter 6: A visual history of Jean Perrin's Brownian motion curves". In Daston, Lorraine; Lunbeck, Elizabeth (eds.). Histories of Scientific Observation (PDF). The University of Chicago Press.
  15. ^ Bigg, Charlotte (2008). "Evident atoms: visuality in Jean Perrin's Brownian motion research" (PDF). Studies in History and Philosophy of Science Part A. 39 (3): 312–322. doi:10.1016/j.shpsa.2008.06.003.
  16. ^ Henri, Victor (1908). "Étude cinématographique des mouvements browniens". C r Hebd Seances Acad Sci (146): 1024-1026.
  17. ^ Landecker, Hannah (2005). "Cellular Features: Microcinematography and Film Theory". Critical Inquiry. 31 (4). University of Chicago Press: 903–937. doi:10.1086/444519.
  18. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 19974144, please use {{cite journal}} with |pmid=19974144 instead.
  19. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 15009025, please use {{cite journal}} with |pmid=15009025 instead.
  20. ^ Rosenberger, Heinz (1929). "Micro-Cinematography in Medical Research". J DENT RES. 9 (3): 343–352. doi:10.1177/00220345290090030501.
  21. ^ "Warren H. (Warren Harmon) Lewis papers, ca. 1913-1964". American Philosophical Society. Retrieved 2011-04-24.
  22. ^ Hoyos-Flight, Monica. "Milestone 2: Nature Milestones in Cytoskeleton". Nature Publishing Group.
  23. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 13083622, please use {{cite journal}} with |pmid=13083622 instead.
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