K562 cells: Difference between revisions
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| id = }}</ref> as they lack the MHC complex required to inhibit NK activity.<ref name="drexler2000" /> They also lack any trace of [[Epstein-Barr virus]] and other herpesviruses. In addition to the [[Philadelphia chromosome]] they also exhibit a second reciprocal translocation between the long arm of [[chromosome]] 15 with chromosome 17.<ref name="lozzio75" /> |
| id = }}</ref> as they lack the [[Major histocompatibility complex|MHC]] complex required to inhibit NK activity.<ref name="drexler2000" /> They also lack any trace of [[Epstein-Barr virus]] and other herpesviruses. In addition to the [[Philadelphia chromosome]] they also exhibit a second reciprocal translocation between the long arm of [[chromosome]] 15 with chromosome 17.<ref name="lozzio75" /> |
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Two sub-lines are available which express MHC class-I A2<ref name="britten2002"> |
Two sub-lines are available which express MHC class-I A2<ref name="britten2002"> |
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Revision as of 06:10, 7 April 2014
K562 cells were the first human immortalised myelogenous leukemia line to be established. K562 cells are of the erythroleukemia type, and the line is derived from a 53 year old female CML patient in blast crisis.[1][2] The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes[3] and erythrocytes.[4]
In culture they exhibit much less clumping than many other suspension lines, presumably due to the downregulation of surface adhesion molecules by bcr:abl.[citation needed] K562s can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes[5] and are easily killed by natural killer cells[6] as they lack the MHC complex required to inhibit NK activity.[2] They also lack any trace of Epstein-Barr virus and other herpesviruses. In addition to the Philadelphia chromosome they also exhibit a second reciprocal translocation between the long arm of chromosome 15 with chromosome 17.[1]
Two sub-lines are available which express MHC class-I A2[7] and A3.[8]
References
- ^ a b Lozzio, C.B.; Lozzio, B.B. (1975), "Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome", Blood, 45 (3): 321–34, PMID 163658
- ^ a b Drexler, H.G. (2000), The Leukemia-Lymphoma Cell Line Factsbook, San Diego: Academic Press
- ^ Klein, E.; Ben-Bassat, H.; Neumann, H.; Ralph, P.; Zeuthen, J.; Polliack, A.; Vánky, F. (1976), "Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia", International Journal of Cancer, 18 (4): 421–31, doi:10.1002/ijc.2910180405, PMID 789258
- ^ Andersson, L.C.; Nilsson, K.; Gahmberg, C.G. (1979), "K562 - A human erythroleukemic cell line", International Journal of Cancer, 23 (2): 143–7, doi:10.1002/ijc.2910230202, PMID 367973
- ^ Lozzio, B.B.; Lozzio, C.B.; Bamberger, E.G.; Feliu, A.S. (1981), "A multipotential leukemia cell line (K-562) of human origin", Proceedings of the Society for Experimental Biology and Medicine, 166 (4): 546–50, PMID 7194480
- ^ Lozzio, B.B.; Lozzio, C.B. (1979), "Properties and usefulness of the original K-562 human myelogenous leukemia cell line", Leukemia Research, 3 (6): 363–70, doi:10.1016/0145-2126(79)90033-X, PMID 95026
- ^ Britten, C.M.; Meyer, R.G.; Kreer, T.; Drexler, I.; Wölfel, T.; Herr, W. (2002), "The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8(+) T lymphocytes in IFN-gamma ELISPOT assays", Journal of Immunological Methods, 259 (1–2): 95–110, doi:10.1016/S0022-1759(01)00499-9, PMID 11730845
- ^ Clark, R.E.; Dodi, I.A.; Hill, S.C.; Lill, J.R.; Aubert, G.; Macintyre, A.R.; Rojas, J.; Bourdon, A.; et al. (2001), "Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein", Blood, 98 (10): 2887–93, doi:10.1182/blood.V98.10.2887, PMID 11698267
External links
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