ColE1: Difference between revisions

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ColE1 is a plasmid found in bacteria. Its name derives from the fact that it carries a gene for colicin E1 (the cea gene). It also codes for immunity from this product with the imm gene. In addition the plasmid has a series of mobility (mob) genes.

Replication

ColE1 replication begins at the oriV origin. 555bp upstream from this point, RNA polymerase-initiated transcription of RNAII which acts as a pre-primer and begins the synthesis of the leader strand. The transcript folds into a secondary structure which stabilises the interaction between the nascent RNA and the origin's DNA. This hybrid is attached by RNase H, which cleaves the RNA strand exposing a 3' hydroxyl group. This allows the extension of the leading strand by DNA Polymerase I. Lagging strand synthesis is later initiated by a primase encoded by the host cell.

Copy number control

RNAI is a counter-transcript to a section of RNAII and so binds to its 5' end. This alters the folding of RNAII so that the DNA-RNA hybrid is not stabilised and cleavage does not occur. This ensures that at high copy numbers (and so high RNAI concentration), replication is slowed down. Rom is a secondary replication repressor, it stabilises the RNAI-RNAII hybrid. Rom may be especially important at preventing runaway replication at slow growth rates.

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 ==Replication==
-ColE1 replication begins at the ''oriV'' origin.  555bp upstream from this point, RNA polymerase initiated transcription of RNAII which acts as a pre-primer and begins the synthesis of the [[DNA strand|leader strand]].  The transcript folds into a secondary structure which stabilises the interaction between the nascent RNA and the origin's DNA.  This hybrid is attached by [[RNase H]], which cleaves the RNA strand exposing a 3' hydroxyl group.  This allows the extension of the leading strand by DNA Polymerase I.  Lagging strand synthesis is later initiated by a primase encoded by the host cell.
+ColE1 replication begins at the ''oriV'' origin.  555bp upstream from this point, RNA polymerase-initiated transcription of RNAII which acts as a pre-primer and begins the synthesis of the [[DNA strand|leader strand]].  The transcript folds into a secondary structure which stabilises the interaction between the nascent RNA and the origin's DNA.  This hybrid is attached by [[RNase H]], which cleaves the RNA strand exposing a 3' hydroxyl group.  This allows the extension of the leading strand by DNA Polymerase I.  Lagging strand synthesis is later initiated by a primase encoded by the host cell.
 ==Copy number control==