Archease: Difference between revisions
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In molecular biology, the |
In molecular biology, the ''archease''' [[Protein family|superfamily]] of [[protein]]s are represented in all three [[Domain (biology)|domains]] of [[life]]. Archease [[genes]] are generally located adjacent to [[gene]]s encoding [[protein]]s involved in DNA or RNA processing and therefore been predicted to be modulators or [[chaperone (protein)|chaperones]] involved in DNA or RNA [[metabolism#Regulation and control|metabolism]]. Many of the roles of archeases remain to be established experimentally. |
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The function of one of the archeases from the hyperthermophile ''Pyrococcus abyssi'' has been determined. The gene encoding the archease (PAB1946) is located in a bicistronic [[operon]] immediately [[upstream and downstream (DNA)|upstream]] from a second [[open reading frame]] (PAB1947), which encodes a tRNA m5C [[methyltransferase]]. The [[methyl]] [[transferase]] [[catalysis|catalyses]] m5C formation at several [[cytosine]]'s within tRNAs with preference for C49; the specificity of the methyltransferase reaction being increased by the archease. The archease exists in monomeric and oligomeric states, with only the oligomeric forms able to [[Molecular binding|bind]] the methyltransferase. [[Binding (molecular)|Binding]] prevents [[protein aggregation|aggregation]] and hinders dimerisation of the methyltransferase-tRNA [[Protein complex|complex]].<ref name="pmid17470432">{{cite journal | author = Auxilien S, El Khadali F, Rasmussen A, Douthwaite S, Grosjean H | title = Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase | journal = J. Biol. Chem. | volume = 282 | issue = 26 | pages = 18711â21 |date=June 2007 | pmid = 17470432 | doi = 10.1074/jbc.M607459200 | url = }}</ref> |
The function of one of the archeases from the hyperthermophile ''Pyrococcus abyssi'' has been determined. The gene encoding the archease (PAB1946) is located in a bicistronic [[operon]] immediately [[upstream and downstream (DNA)|upstream]] from a second [[open reading frame]] (PAB1947), which encodes a tRNA m5C [[methyltransferase]]. The [[methyl]] [[transferase]] [[catalysis|catalyses]] m5C formation at several [[cytosine]]'s within tRNAs with preference for C49; the specificity of the methyltransferase reaction being increased by the archease. The archease exists in monomeric and oligomeric states, with only the oligomeric forms able to [[Molecular binding|bind]] the methyltransferase. [[Binding (molecular)|Binding]] prevents [[protein aggregation|aggregation]] and hinders dimerisation of the methyltransferase-tRNA [[Protein complex|complex]].<ref name="pmid17470432">{{cite journal | author = Auxilien S, El Khadali F, Rasmussen A, Douthwaite S, Grosjean H | title = Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase | journal = J. Biol. Chem. | volume = 282 | issue = 26 | pages = 18711â21 |date=June 2007 | pmid = 17470432 | doi = 10.1074/jbc.M607459200 | url = }}</ref> |
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Revision as of 06:52, 24 February 2015
| Archease | |||||||||
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 solution structure of methanobacterium thermoautotrophicum protein 1598. ontario centre for structural proteomics target mth1598_1_140; northeast structural genomics target tt6 | |||||||||
| Identifiers | |||||||||
| Symbol | Archease | ||||||||
| Pfam | PF01951 | ||||||||
| InterPro | IPR002804 | ||||||||
| SCOP2 | 1jw3 / SCOPe / SUPFAM | ||||||||
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In molecular biology, the archease' superfamily of proteins are represented in all three domains of life. Archease genes are generally located adjacent to genes encoding proteins involved in DNA or RNA processing and therefore been predicted to be modulators or chaperones involved in DNA or RNA metabolism. Many of the roles of archeases remain to be established experimentally.
The function of one of the archeases from the hyperthermophile Pyrococcus abyssi has been determined. The gene encoding the archease (PAB1946) is located in a bicistronic operon immediately upstream from a second open reading frame (PAB1947), which encodes a tRNA m5C methyltransferase. The methyl transferase catalyses m5C formation at several cytosine's within tRNAs with preference for C49; the specificity of the methyltransferase reaction being increased by the archease. The archease exists in monomeric and oligomeric states, with only the oligomeric forms able to bind the methyltransferase. Binding prevents aggregation and hinders dimerisation of the methyltransferase-tRNA complex.[1]
The function of this family of archeases as chaperones is supported by structural analysis of the archease from Methanobacterium thermoautotrophicum, which shows homology to heat shock protein 33, a chaperone protein that inhibits the aggregation of partially denatured proteins.[2]
References
- ^ Auxilien S, El Khadali F, Rasmussen A, Douthwaite S, Grosjean H (June 2007). "Archease from Pyrococcus abyssi improves substrate specificity and solubility of a tRNA m5C methyltransferase". J. Biol. Chem. 282 (26): 18711â21. doi:10.1074/jbc.M607459200. PMID 17470432.
{{cite journal}}: C1 control character in|pages=at position 7 (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link) - ^ Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH (February 2002). "An NMR approach to structural proteomics". Proc. Natl. Acad. Sci. U.S.A. 99 (4): 1825â30. doi:10.1073/pnas.042684599. PMC 122278. PMID 11854485.
{{cite journal}}: C1 control character in|pages=at position 6 (help)CS1 maint: multiple names: authors list (link)