User:Cpozlov: Difference between revisions
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I intend to add to the information describing what a nick is (molecular structure as well as function), and create a section for mismatch repair to add to the pre-existing information about the nick’s role. I’d like to add another section about how and when DNA ligase interacts with nicks. |
I intend to add to the information describing what a nick is (molecular structure as well as function), and create a section for mismatch repair to add to the pre-existing information about the nick’s role. I’d like to add another section about how and when DNA ligase interacts with nicks. |
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'''Proposed Headings:''' |
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== Role In Mismatch Repair == |
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== Nick Ligation == |
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'''Example sentences that will be in the article:''' |
'''Example sentences that will be in the article:''' |
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Single-stranded and double stranded nicks are repaired by DNA ligase, an enzyme with similar structural elements to DNA polymerase.<ref>{{cite journal|last1=Cherepanov|first1=Alexei V.|last2=de Vries|first2=Simon|title=Dynamic mechanism of nick recognition by DNA ligase|journal=European Journal of Biochemistry|date=11 December 2002|volume=269|issue=24|pages=5993–5999|url=http://onlinelibrary.wiley.com/doi/10.1046/j.1432-1033.2002.03309.x/epdf}}</ref> During DNA replication, DNA ligase creates a complex with the nicked DNA between Okazaki fragments. The complex lets DNA ligase momentarily unwind part of the double-helix to expose the nick for ligation.<ref>{{cite journal|last1=Pascal|first1=John M.|last2=O'Brien|first2=Patrick J.|last3=Tomkinson|first3=Alan E.|last4=Ellenberger|first4=Tom|title=Human DNA ligase I completely encircles and partially unwinds nicked DNA|journal=Nature|date=25 November 2004|volume=432|pages=473-478|url=http://www.nature.com/nature/journal/v432/n7016/full/nature03082.html}}</ref> |
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Single-stranded and double stranded nicks are repaired by DNA ligase, an enzyme with similar structural elements to DNA polymerase. DNA ligase performs nick repair after random DNA damage, as well as when the formation of nicks is deliberate. During DNA replication, DNA polymerase creates Okazaki fragments that have nicks between them, which are ligated by DNA ligase. |
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Revision as of 21:51, 15 October 2015
Chris P from the University of Western Ontario Bio 3595A Advanced Genetics class
| This user is a student editor in Wikipedia:Wiki_Ed/Western_University/Bio3595A_Advanced_Genetics_(Fall_2015). Student assignments should always be carried out using a course page set up by the instructor. It is usually best to develop assignments in your sandbox. After evaluation, the additions may go on to become a Wikipedia article or be published in an existing article. |
Training for Students complete!
What the stub already contains: The article currently gives a basic overview of what a nick is in DNA. It also describes the role that nicks play in DNA mismatch repair.
I intend to add to the information describing what a nick is (molecular structure as well as function), and create a section for mismatch repair to add to the pre-existing information about the nick’s role. I’d like to add another section about how and when DNA ligase interacts with nicks.
Proposed Headings:
Role In Mismatch Repair
Nick Ligation
Example sentences that will be in the article:
Single-stranded and double stranded nicks are repaired by DNA ligase, an enzyme with similar structural elements to DNA polymerase.[1] During DNA replication, DNA ligase creates a complex with the nicked DNA between Okazaki fragments. The complex lets DNA ligase momentarily unwind part of the double-helix to expose the nick for ligation.[2]
Cpozlov (talk) 05:36, 21 September 2015 (UTC)
- ^ Cherepanov, Alexei V.; de Vries, Simon (11 December 2002). "Dynamic mechanism of nick recognition by DNA ligase". European Journal of Biochemistry. 269 (24): 5993–5999.
- ^ Pascal, John M.; O'Brien, Patrick J.; Tomkinson, Alan E.; Ellenberger, Tom (25 November 2004). "Human DNA ligase I completely encircles and partially unwinds nicked DNA". Nature. 432: 473–478.