Colcemid: Difference between revisions
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==Mechanism of Action== |
==Mechanism of Action== |
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Colcemid is a microtubule-depolymerizing drug like [[vinblastine]]. It acts by two distinct mechanisms. At very low concentration it binds to microtubule plus end to suppress microtubule dynamics.<ref>{{cite journal |last1=Jordan |first1=Mary Ann |last2=Wilson |first2=Leslie |title=Microtubules as a target for anticancer drugs |journal=Nature reviews. Cancer |volume=4 |issue=4 |pages=253–65 |year=2004 |pmid=15057285 |doi=10.1038/nrc1317}}</ref> Recent study has found at higher concentration colcemid can promote microtubule detachment from microtubule organizing center. Detached microtubules with unprotected minus end |
Colcemid is a microtubule-depolymerizing drug like [[vinblastine]]. It acts by two distinct mechanisms. At very low concentration it binds to microtubule plus end to suppress microtubule dynamics.<ref>{{cite journal |last1=Jordan |first1=Mary Ann |last2=Wilson |first2=Leslie |title=Microtubules as a target for anticancer drugs |journal=Nature reviews. Cancer |volume=4 |issue=4 |pages=253–65 |year=2004 |pmid=15057285 |doi=10.1038/nrc1317}}</ref> Recent study has found at higher concentration colcemid can promote microtubule detachment from microtubule organizing center. Detached microtubules with unprotected minus end depolymerize with time. Cytotoxicity of the cells seems to correlate better with [[microtubule]] detachment.<ref name=pmid20696757>{{cite journal |last1=Yang |first1=Hailing |last2=Ganguly |first2=Anutosh |last3=Cabral |first3=Fernando |title=Inhibition of Cell Migration and Cell Division Correlates with Distinct Effects of Microtubule Inhibiting Drugs |journal=The Journal of Biological Chemistry |volume=285 |issue=42 |pages=32242–50 |year=2010 |pmid=20696757 |pmc=2952225 |doi=10.1074/jbc.M110.160820}}</ref> Lower concentration affect microtubule dynamics and cell migration.<ref name=pmid20696757/> |
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== Uses == |
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Colcemid is used primarily for scientific research in cells. It is used in a variety of ways, however, until recently, was used mostly for the study of [[mitosis]] in cells. For example, microtubules are necessary for the splitting of cells. More importantly, the movement of chromosomes during the M phase. Colcemid inhibition of microtubules causes [[aneuploidy]] in mitotic cells where the microtubules fall apart or are suppressed before they can complete their function of pulling chromosomes into the daughter cell, or non- dysjunction of chromosomes<ref>{{Cite journal|title = Mitotic slippage underlies the relationship between p53 dysfunction and the induction of large micronuclei by colcemid|url = http://mutage.oxfordjournals.org/content/28/4/457|journal = Mutagenesis|date = 2013-07-01|issn = 0267-8357|pmid = 23702691|pages = 457-464|volume = 28|issue = 4|doi = 10.1093/mutage/get021|language = en|first = Kiyohiro|last = Hashimoto|first2 = Takeshi|last2 = Todo}}</ref> |
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==References== |
==References== |
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Revision as of 17:48, 14 November 2015
 | This article needs additional citations for verification. (September 2010) |
Used in cytogenetics, colcemid, also known as demecolcine, is related to colchicine but it is less toxic. It depolymerises microtubules and limits microtubule formation (inactivates spindle fibre formation), thus arresting cells in metaphase and allowing cell harvest and karyotyping to be performed.
Mechanism of Action
Colcemid is a microtubule-depolymerizing drug like vinblastine. It acts by two distinct mechanisms. At very low concentration it binds to microtubule plus end to suppress microtubule dynamics.[1] Recent study has found at higher concentration colcemid can promote microtubule detachment from microtubule organizing center. Detached microtubules with unprotected minus end depolymerize with time. Cytotoxicity of the cells seems to correlate better with microtubule detachment.[2] Lower concentration affect microtubule dynamics and cell migration.[2]
Uses
Colcemid is used primarily for scientific research in cells. It is used in a variety of ways, however, until recently, was used mostly for the study of mitosis in cells. For example, microtubules are necessary for the splitting of cells. More importantly, the movement of chromosomes during the M phase. Colcemid inhibition of microtubules causes aneuploidy in mitotic cells where the microtubules fall apart or are suppressed before they can complete their function of pulling chromosomes into the daughter cell, or non- dysjunction of chromosomes[3]
References
- ^ Jordan, Mary Ann; Wilson, Leslie (2004). "Microtubules as a target for anticancer drugs". Nature reviews. Cancer. 4 (4): 253–65. doi:10.1038/nrc1317. PMID 15057285.
- ^ a b Yang, Hailing; Ganguly, Anutosh; Cabral, Fernando (2010). "Inhibition of Cell Migration and Cell Division Correlates with Distinct Effects of Microtubule Inhibiting Drugs". The Journal of Biological Chemistry. 285 (42): 32242–50. doi:10.1074/jbc.M110.160820. PMC 2952225. PMID 20696757.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Hashimoto, Kiyohiro; Todo, Takeshi (2013-07-01). "Mitotic slippage underlies the relationship between p53 dysfunction and the induction of large micronuclei by colcemid". Mutagenesis. 28 (4): 457–464. doi:10.1093/mutage/get021. ISSN 0267-8357. PMID 23702691.