Golgi apparatus: Difference between revisions
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[[File:Blausen 0435 GolgiApparatus.png|thumb|left|3D rendering of Golgi apparatus]] |
[[File:Blausen 0435 GolgiApparatus.png|thumb|left|3D rendering of Golgi apparatus]] |
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[[File:Golgi apparatus (borderless version)-en.svg|thumb|400px|Diagram of a single "stack" of Golgi]] |
[[File:Golgi apparatus (borderless version)-en.svg|thumb|400px|Diagram of a single "stack" of Golgi]] |
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In most [[eukaryote]]s, the Golgi apparatus is made up of a series of compartments consisting of two main networks: the ''cis'' Golgi network (CGN) and the ''trans'' Golgi network (TGN). The CGN is a collection of fused, flattened membrane-enclosed disks known as [[cisternae]] (singular: ''cisterna''), originating from vesicular clusters that bud off the [[endoplasmic reticulum]]. A mammalian cell typically contains 40 to 100 stacks.<ref name="pmid18385516">{{cite journal |vauthors=Duran JM, Kinseth M, Bossard C, Rose DW, Polishchuk R, Wu CC, Yates J, Zimmerman T, Malhotra V | title = The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis. | journal = Mol. Biol. Cell. | volume = 19 | issue = 6 | pages = 2579–87 | year = 2008| pmid = 18385516 | doi = 10.1091/mbc.E07-10-0998 | pmc=2397314}}</ref> Between four and eight cisternae are usually present in a stack; however, in some [[protists]] as many as sixty cisternae have been observed.<ref name="url_molexpress"/> This collection of cisternae is broken down into ''cis'', medial, and ''trans'' compartments. The TGN is the final cisternal structure, from which [[protein]]s are packaged into [[Vesicle (biology and chemistry)|vesicle]]s destined to [[lysosome]]s, secretory vesicles, or the cell surface. The TGN is usually positioned adjacent to the stacks of the Golgi apparatus, but can also be separate from the stacks. The TGN may act as an early [[endosome]] in [[yeast]] and [[plant]]s.<ref name="pmid20605430"/> |
In most [[eukaryote]]s, the Golgi apparatus is made up of a series of compartments consisting of two main networks: the ''cis'' Golgi network (CGN) and the ''trans'' Golgi network (TGN). The CGN is a collection of fused, flattened membrane-enclosed disks known as [[cisternae]] (singular: ''cisterna'', also called "dictyosomes"), originating from vesicular clusters that bud off the [[endoplasmic reticulum]]. A mammalian cell typically contains 40 to 100 stacks.<ref name="pmid18385516">{{cite journal |vauthors=Duran JM, Kinseth M, Bossard C, Rose DW, Polishchuk R, Wu CC, Yates J, Zimmerman T, Malhotra V | title = The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis. | journal = Mol. Biol. Cell. | volume = 19 | issue = 6 | pages = 2579–87 | year = 2008| pmid = 18385516 | doi = 10.1091/mbc.E07-10-0998 | pmc=2397314}}</ref> Between four and eight cisternae are usually present in a stack; however, in some [[protists]] as many as sixty cisternae have been observed.<ref name="url_molexpress"/> This collection of cisternae is broken down into ''cis'', medial, and ''trans'' compartments. The TGN is the final cisternal structure, from which [[protein]]s are packaged into [[Vesicle (biology and chemistry)|vesicle]]s destined to [[lysosome]]s, secretory vesicles, or the cell surface. The TGN is usually positioned adjacent to the stacks of the Golgi apparatus, but can also be separate from the stacks. The TGN may act as an early [[endosome]] in [[yeast]] and [[plant]]s.<ref name="pmid20605430"/> |
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There are structural and organizational differences in the Golgi apparatus among eukaryotes. In some yeasts, Golgi stacking is not observed. ''[[Pichia pastoris]]'' does have stacked Golgi, while ''[[Saccharomyces cerevisiae]]'' does not.<ref name="pmid20605430"/> In plants, the individual stacks of the Golgi apparatus seem to operate independently.<ref name="pmid20605430"/> |
There are structural and organizational differences in the Golgi apparatus among eukaryotes. In some yeasts, Golgi stacking is not observed. ''[[Pichia pastoris]]'' does have stacked Golgi, while ''[[Saccharomyces cerevisiae]]'' does not.<ref name="pmid20605430"/> In plants, the individual stacks of the Golgi apparatus seem to operate independently.<ref name="pmid20605430"/> |
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Revision as of 00:06, 31 January 2017
| Cell biology | |
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| Animal cell diagram | |
 Components of a typical animal cell:
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The Golgi apparatus (/ˈɡoʊldʒiː/), also known as the Golgi complex, Golgi body, or simply the Golgi, is an organelle found in most eukaryotic cells.[1] It was identified in 1897 by the Italian scientist Camillo Golgi and named after him in 1898.[2]
Part of the cellular endomembrane system, the Golgi apparatus packages proteins into membrane-bound vesicles inside the cell before the vesicles are sent to their destination. The Golgi apparatus resides at the intersection of the secretory, lysosomal, and endocytic pathways. It is of particular importance in processing proteins for secretion, containing a set of glycosylation enzymes that attach various sugar monomers to proteins as the proteins move through the apparatus.
Discovery
Owing to its large size and distinctive structure, the Golgi apparatus was one of the first organelles to be discovered and observed in detail. It was discovered in 1898 by Italian physician Camillo Golgi during an investigation of the nervous system.[3][2] After first observing it under his microscope, he termed the structure the internal reticular apparatus. Some doubted the discovery at first, arguing that the appearance of the structure was merely an optical illusion created by the observation technique used by Golgi. With the development of modern microscopes in the 20th century, the discovery was confirmed.[4] Early references to the Golgi referred to it by various names including the "Golgi–Holmgren apparatus", "Golgi–Holmgren ducts", and "Golgi–Kopsch apparatus".[2] The term "Golgi apparatus" was used in 1910 and first appeared in the scientific literature in 1913.[2]
Subcellular localization
Among eukaryotes, the subcellular localization of the Golgi apparatus differs. In mammals, a single Golgi apparatus complex is usually located near the cell nucleus, close to the centrosome. Tubular connections are responsible for linking the stacks together. Localization and tubular connections of the Golgi apparatus are dependent on microtubules. If microtubules are experimentally depolymerized, then the Golgi apparatus loses connections and becomes individual stacks throughout the cytoplasm.[5] In yeast, multiple Golgi apparatuses are scattered throughout the cytoplasm (as observed in Saccharomyces cerevisiae). In plants, Golgi stacks are not concentrated at the centrosomal region and do not form Golgi ribbons.[6] Organization of the plant Golgi depends on actin cables and not microtubules.[6] The common feature among Golgi is that they are adjacent to endoplasmic reticulum (ER) exit sites.[7]
Structure
-en.svg)
In most eukaryotes, the Golgi apparatus is made up of a series of compartments consisting of two main networks: the cis Golgi network (CGN) and the trans Golgi network (TGN). The CGN is a collection of fused, flattened membrane-enclosed disks known as cisternae (singular: cisterna, also called "dictyosomes"), originating from vesicular clusters that bud off the endoplasmic reticulum. A mammalian cell typically contains 40 to 100 stacks.[8] Between four and eight cisternae are usually present in a stack; however, in some protists as many as sixty cisternae have been observed.[4] This collection of cisternae is broken down into cis, medial, and trans compartments. The TGN is the final cisternal structure, from which proteins are packaged into vesicles destined to lysosomes, secretory vesicles, or the cell surface. The TGN is usually positioned adjacent to the stacks of the Golgi apparatus, but can also be separate from the stacks. The TGN may act as an early endosome in yeast and plants.[6]
There are structural and organizational differences in the Golgi apparatus among eukaryotes. In some yeasts, Golgi stacking is not observed. Pichia pastoris does have stacked Golgi, while Saccharomyces cerevisiae does not.[6] In plants, the individual stacks of the Golgi apparatus seem to operate independently.[6]
The Golgi apparatus tends to be larger and more numerous in cells that synthesize and secrete large amounts of substances; for example, the antibody-secreting plasma B cells of the immune system have prominent Golgi complexes.
In all eukaryotes, each cisternal stack has a cis entry face and a trans exit face. These faces are characterized by unique morphology and biochemistry.[9] Within individual stacks are assortments of enzymes responsible for selectively modifying protein cargo. These modifications influence the fate of the protein. The compartmentalization of the Golgi apparatus is advantageous for separating enzymes, thereby maintaining consecutive and selective processing steps: enzymes catalyzing early modifications are gathered in the cis face cisternae, and enzymes catalyzing later modifications are found in trans face cisternae of the Golgi stacks.[5][9]
Function
The Golgi apparatus is a major collection and dispatch station of protein products received from the endoplasmic reticulum (ER). Proteins synthesized in the ER are packaged into vesicles, which then fuse with the Golgi apparatus. These cargo proteins are modified and destined for secretion via exocytosis or for use in the cell. In this respect, the Golgi can be thought of as similar to a post office: it packages and labels items which it then sends to different parts of the cell or to the extracellular space. The Golgi apparatus is also involved in lipid transport and lysosome formation.[10]
The structure and function of the Golgi apparatus are intimately linked. Individual stacks have different assortments of enzymes, allowing for progressive processing of cargo proteins as they travel from the cist ernae to the trans Golgi face.[5][9] Enzymatic reactions within the Golgi stacks occur exclusively near its membrane surfaces, where enzymes are anchored. This feature is in contrast to the ER, which has soluble proteins and enzymes in its lumen. Much of the enzymatic processing is post-translational modification of proteins. For example, phosphorylation of oligosaccharides on lysosomal proteins occurs in the early CGN.[5] Cis cisterna are associated with the removal of mannose residues.[5][9] Removal of mannose residues and addition of N-acetylglucosamine occur in medial cisternae.[5] Addition of galactose and sialic acid occurs in the trans cisternae.[5] Sulfation of tyrosines and carbohydrates occurs within the TGN.[5] Other general post-translational modifications of proteins include the addition of carbohydrates (glycosylation)[11] and phosphates (phosphorylation). Protein modifications may form a signal sequence that determines the final destination of the protein. For example, the Golgi apparatus adds a mannose-6-phosphate label to proteins destined for lysosomes. Another important function of the Golgi apparatus is in the formation of proteoglycans. Enzymes in the Golgi append proteins to glycosaminoglycans, thus creating proteoglycans.[12] Glycosaminoglycans are long unbranched polysaccharide molecules present in the extracellular matrix of animals.
Vesicular transport
The vesicles that leave the rough endoplasmic reticulum are transported to the cis face of the Golgi apparatus, where they fuse with the Golgi membrane and empty their contents into the lumen. Once inside the lumen, the molecules are modified, then sorted for transport to their next destinations.
Those proteins destined for areas of the cell other than either the endoplasmic reticulum or the Golgi apparatus are moved through the Golgi cisternae towards the trans face, to a complex network of membranes and associated vesicles known as the trans-Golgi network (TGN). This area of the Golgi is the point at which proteins are sorted and shipped to their intended destinations by their placement into one of at least three different types of vesicles, depending upon the signal sequence they carry.
| Types | Description | Example |
|---|---|---|
| Exocytotic vesicles (constitutive) | Vesicle contains proteins destined for extracellular release. After packaging, the vesicles bud off and immediately move towards the plasma membrane, where they fuse and release the contents into the extracellular space in a process known as constitutive secretion. | Antibody release by activated plasma B cells |
| Secretory vesicles (regulated) | Vesicles contain proteins destined for extracellular release. After packaging, the vesicles bud off and are stored in the cell until a signal is given for their release. When the appropriate signal is received they move toward the membrane and fuse to release their contents. This process is known as regulated secretion. | Neurotransmitter release from neurons |
| Lysosomal vesicles | Vesicles contain proteins and ribosomes destined for the lysosome, a degradative organelle containing many acid hydrolases, or to lysosome-like storage organelles. These proteins include both digestive enzymes and membrane proteins. The vesicle first fuses with the late endosome, and the contents are then transferred to the lysosome via unknown mechanisms. | Digestive proteases destined for the lysosome |
Current models of vesicular transport and trafficking
Model 1: Anterograde vesicular transport between stable compartments
- In this model, the Golgi is viewed as a set of stable compartments that work together. Each compartment has a unique collection of enzymes that work to modify protein cargo. Proteins are delivered from the ER to the cis face using COPII-coated vesicles. Cargo then progress toward the trans face in COPI-coated vesicles. This model proposes that COPI vesicles move in two directions: anterograde vesicles carry secretory proteins, while retrograde vesicles recycle Golgi-specific trafficking proteins.[13]
- Strengths: The model explains observations of compartments, polarized distribution of enzymes, and waves of moving vesicles. It also attempts to explain how Golgi-specific enzymes are recycled.[13]
- Weaknesses: Since the amount of COPI vesicles varies drastically among types of cells, this model cannot easily explain high trafficking activity within the Golgi for both small and large cargoes. Additionally, there is no convincing evidence that COPI vesicles move in both the anterograde and retrograde directions.[13]
- This model was widely accepted from the early 1980s until the late 1990s.[13]
Model 2: Cisternal progression/maturation
- In this model, the fusion of COPII vesicles from the ER begins the formation of the first cis-cisterna of the Golgi stack, which progresses later to become mature TGN cisternae. Once matured, the TGN cisternae dissolves to become secretory vesicles. While this progression occurs, COPI vesicles continually recycle Golgi-specific proteins by delivery from older to younger cisternae. Different recycling patterns may account for the differing biochemistry throughout the Golgi stack. Thus, the compartments within the Golgi are seen as discrete kinetic stages of the maturing Golgi apparatus.[13]
- Strengths: The model addresses the existence of Golgi compartments, as well as differing biochemistry within the cisternae, transport of large proteins, transient formation and disintegration of the cisternae, and retrograde mobility of native Golgi proteins, and it can account for the variability seen in the structures of the Golgi.[13]
- Weaknesses: This model cannot easily explain the observation of fused Golgi networks, tubular connections among cisternae, and differing kinetics of secretory cargo exit.[13]
Model 3: Cisternal progression/maturation with heterotypic tubular transport
- This model is an extension of the cisternal progression/maturation model. It incorporates the existence of tubular connections among the cisternae that form the Golgi ribbon, in which cisternae within a stack are linked. This model posits that the tubules are important for bidirectional traffic in the ER-Golgi system: they allow for fast anterograde traffic of small cargo and/or the retrograde traffic of native Golgi proteins.[13]
- Strengths: This model encompasses the strengths of the cisternal progression/maturation model that also explains rapid trafficking of cargo, and how native Golgi proteins can recycle independently of COPI vesicles.[13]
- Weaknesses: This model cannot explain the transport kinetics of large protein cargo, such as collagen. Additionally, tubular connections are not prevalent in plant cells. The roles that these connections have can be attributed to a cell-specific specialization rather than a universal trait. If the membranes are continuous, that suggests the existence of mechanisms that preserve the unique biochemical gradients observed throughout the Golgi apparatus.[13]
Model 4: Rapid partitioning in a mixed Golgi
- This rapid partitioning model is the most drastic alteration of the traditional vesicular trafficking point of view. Proponents of this model hypothesize that the Golgi works as a single unit, containing domains that function separately in the processing and export of protein cargo. Cargo from the ER move between these two domains, and randomly exit from any level of the Golgi to their final location. This model is supported by the observation that cargo exits the Golgi in a pattern best described by exponential kinetics. The existence of domains is supported by fluorescence microscopy data.[13]
- Strengths: Notably, this model explains the exponential kinetics of cargo exit of both large and small proteins whereas other models cannot.[13]
- Weaknesses: This model cannot explain the transport kinetics of large protein cargo, such as collagen. This model falls short on explaining the observation of discrete compartments and polarized biochemistry of the Golgi cisternae. It also does not explain formation and disintegration of the Golgi network, nor the role of COPI vesicles.[13]
Model 5: Stable compartments as cisternal model progenitors
- This is the most recent model. In this model, the Golgi is seen as a collection of stable compartments defined by Rab (G-protein) GTPases.[13]
- Strengths: This model is consistent with numerous observations and encompasses some of the strengths of the cisternal progression/maturation model. Additionally, what is known of the Rab GTPase roles in mammalian endosomes can help predict putative roles within the Golgi. This model is unique in that it can explain the observation of "megavesicle" transport intermediates.[13]
- Weaknesses: This model does not explain morphological variations in the Golgi apparatus, nor define a role for COPI vesicles. This model does not apply well for plants, algae, and fungi in which individual Golgi stacks are observed (transfer of domains between stacks is not likely). Additionally, megavesicles are not established to be intra-Golgi transporters.[13]
Though there are multiple models that attempt to explain vesicular traffic throughout the Golgi, no individual model can independently explain all observations of the Golgi apparatus. Currently, the cisternal progression/maturation model is the most accepted among scientists, accommodating many observations across eukaryotes. The other models are still important in framing questions and guiding future experimentation. Among the fundamental unanswered questions are the directionality of COPI vesicles and role of Rab GTPases in modulating protein cargo traffic.[13]
Brefeldin A
Brefeldin A (BFA) is a fungal metabolite used experimentally to disrupt the secretion pathway as a method of testing Golgi function.[14] BFA blocks the activation of some ADP-ribosylation factors (ARFs).[15] ARFs are small GTPases which regulate vesicular trafficking through the binding of COPs to endosomes and the Golgi.[15] BFA inhibits the function of several guanine nucleotide exchange factors (GEFs) that mediate GTP-binding of ARFs.[15] Treatment of cells with BFA thus disrupts the secretion pathway, promoting disassembly of the Golgi apparatus and distributing Golgi proteins to the endosomes and ER.[14][15]
References
- ^ Pavelk M, Mironov AA (2008). The Golgi Apparatus: State of the art 110 years after Camillo Golgi's discovery. Berlin: Springer. ISBN 3-211-76309-0.[page needed]
- ^ a b c d Fabene PF, Bentivoglio M (1998). "1898–1998: Camillo Golgi and "the Golgi": one hundred years of terminological clones". Brain Res. Bull. 47 (3): 195–8. doi:10.1016/S0361-9230(98)00079-3. PMID 9865849.
- ^ Golgi, C. (1898). Intorno alla struttura delle cellule nervose. Bollettino della Società Medico-Chirurgica di Pavia 13(1): 316.
- ^ a b Davidson MW (2004-12-13). "The Golgi Apparatus". Molecular Expressions. Florida State University. Retrieved 2010-09-20.
- ^ a b c d e f g h Alberts, Bruce; et al. Molecular Biology of the Cell. Garland Publishing. ISBN 978-0-8153-1619-0.
- ^ a b c d e Nakano A, Luini A (2010). "Passage through the Golgi". Curr Opin Cell Biol. 22 (4): 471–8. doi:10.1016/j.ceb.2010.05.003. PMID 20605430.
- ^ Suda Y, Nakano A (2012). "The yeast Golgi apparatus". Traffic. 13 (4): 505–10. doi:10.1111/j.1600-0854.2011.01316.x. PMID 22132734.
- ^ Duran JM, Kinseth M, Bossard C, Rose DW, Polishchuk R, Wu CC, Yates J, Zimmerman T, Malhotra V (2008). "The role of GRASP55 in Golgi fragmentation and entry of cells into mitosis". Mol. Biol. Cell. 19 (6): 2579–87. doi:10.1091/mbc.E07-10-0998. PMC 2397314. PMID 18385516.
- ^ a b c d Day KJ, Staehelin LA, Glick BS (2013). "A three-stage model of Golgi structure and function". Histochem Cell Biol . 140 (3): 239–49. doi:10.1007/s00418-013-1128-3. PMC 3779436. PMID 23881164.
- ^ Campbell, Neil A (1996). Biology (4 ed.). Menlo Park, CA: Benjamin/Cummings. pp. 122, 123. ISBN 0-8053-1957-3.
- ^ William G. Flynne (2008). Biotechnology and Bioengineering. Nova Publishers. pp. 45–. ISBN 978-1-60456-067-1. Retrieved 13 November 2010.
- ^ Prydz K, Dalen KT (January 2000). "Synthesis and sorting of proteoglycans". J. Cell. Sci. 113. Pt 2: 193–205. PMID 10633071.
- ^ a b c d e f g h i j k l m n o p q Glick BS, Luini A (2011). "Models for Golgi traffic: a critical assessment". Cold Spring Harb Perspect Biol. 3 (11): a005215. doi:10.1101/cshperspect.a005215. PMID 21875986.
- ^ a b Marie M, Sannerud R, Avsnes Dale H, Saraste J (2008). "Take the 'A' train: on fast tracks to the cell surface". Cell Mol Life Sci. 65 (18): 2859–74. doi:10.1007/s00018-008-8355-0. PMID 18726174.
- ^ a b c d D'Souza-Schorey C, Chavrier P (2006). "ARF proteins: roles in membrane traffic and beyond". Nat Rev Mol Cell Biol. 7 (5): 347–58. doi:10.1038/nrm1910. PMID 16633337.
External links
