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'''TaqI''' is a [[restriction enzyme]] isolated from the [[bacterium]] ''[[Thermus aquaticus]]'' in 1978.<ref>{{cite journal|date=February 1978| first= S.| last= Sato| title=A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I| journal=J. Biochem. (Tokyo)| volume=83| issue=2| pages=633–5|pmid=204628 }}</ref> It has a [[recognition sequence]] of
'''TaqI''' is a [[restriction enzyme]] isolated from the [[bacterium]] ''[[Thermus aquaticus]]'' in 1978.<ref>{{cite journal|date=February 1978| first= S.| last= Sato| title=A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I| journal=J. Biochem.| volume=83| issue=2| pages=633–5|pmid=204628 }}</ref> It has a [[recognition sequence]] of


5'TCGA
5'TCGA

Revision as of 15:28, 27 August 2017

TaqI is a restriction enzyme isolated from the bacterium Thermus aquaticus in 1978.[1] It has a recognition sequence of

5'TCGA
3'AGCT

and makes the cut

5'---T   CGA---3'
3'---AGC   T---5'

References

  1. ^ Sato, S. (February 1978). "A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I". J. Biochem. 83 (2): 633–5. PMID 204628.