Talk:DNA sequencing

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This article was the subject of a Wiki Education Foundation-supported course assignment, between 12 May 2020 and 22 June 2020. Further details are available on the course page. Student editor(s): Brizileigh (article contribs). Peer reviewers: Cstaheli.

Whoa, where's the "layman's terms"?

Aww, I was hoping to learn how DNA was sequenced, but I reckon if you are an individual that understands "... initiated at a specific site on the template DNA by using a short oligonucleotide 'primer' complementary to the template at that region.", then you probably already know how DNA is sequenced. Common, there's got to be someone out there that is talented enough with words and biology to make this topic accessible to anyone who tries. :) -Tom

I'll come back later and give it a try. I'll leave a note here so I'll remember to come back.Nbauman 19:15, 3 November 2006 (UTC)

This is quandry. Since Wikipedia is an encyclopedia it should be written for senior in high school level. But a senior should know what the four common DNA nucleotides are, that DNA has polarity, what a primer is, and that a primer annealing to a complementary strand could be used to initiate polymerization. But as I write this I can understand. To a molecular biologist DNA sequencing is as straight forward as it gets. To try to explain DNA sequencing and having to explain it all the way down to the basics of DNA polarity is a mind boggling task.

As a 3rd year molecular biology student at a rather prestigious research university I think I can say that DNA sequencing is not "as straight forward as it gets". This page could really use a diagram like the one on the PCR page. Sure, one might be well off to brush up on DNA Replication and PAGE before trying to tackle the intricacies of sequencing, but it might not hurt for the page to be a little clearer on why knowing the length of various segments tells one anything about which nucleotide goes where. As those in the know, we have a responsibility to share our knowledge with others, and I really think that its attitudes like this that have led whatever majority of americans to believe that the world was created in 6 days. So lets be helpful, if only for our own sake ArmyOfFluoride (talk) 05:16, 19 March 2009 (UTC) No such thing as DNA or not, doesn't matter. Be/can be any no matter what and any can be perfect. — Preceding unsigned comment added by VunslK (talk • contribs) 07:51, 28 September 2018 (UTC)

THE REPLICATION AND DETERMINATION OF DNA IN ITS RAW FORM.

If this is possible that a DNA strand can be copied by tRNA mRNA and create a polypeptide sequence, and we know we can replicate it. over the years I have studied this subject and heard the some where someone is mapping the dna sequence of a human being as the dna is all similar obviously not the same or we would all be carbon copies of each other like the bits that make us tall short different eye colour ect. then why do we insist on hacking people open and mending holes in hearts etc if a sample of tissue can be replicated and grown on a mouse back for example how long will it be that it is a simple thing called copy and paste we use it all the time in computers copy the affected area and past a new bit back in this can be put in the form of an injection or hyper spray and as scar tissue is formed over an area of a wound it’s the body’s way of compensating for the loss of information to that area why can’t we do the same.

About the Sequence of the Pieces of DNA

If DNA is destroyed into millions of pieces before reading, how do they know which piece is after the other?--95.10.139.121 (talk) 17:53, 26 May 2015 (UTC)

This is the problem of sequence assembly, either by mapping the pieces to an existing DNA sequence, or by assembling the fragments without any other information (de novo transcriptome assembly). --Amkilpatrick (talk) 19:00, 27 May 2015 (UTC).

Don't you need to sequence overlapping fragments ? (that's how I did it 1978). We were happy when we could read more than 100 bp per sequence. Renebach (talk) 13:06, 22 October 2016 (UTC)

much improved

from the last time I was here; at least, I don't think there are any really stupid errors (sad to say, there were) The whole next gen thing seems a little disorganized and repetitive; perhaps re organizing: by commercial, by date of intro, and non commercial, by date of first real sequence (say 100 bp or bases), and prospsective, no actual sequence data yet

if you talk to real sequencers, they will tell you that post sequence bio informatics is half of the work, so that section needs to be expanded - eg, a sequnce isn't any good if you don't know what to do with it — Preceding unsigned comment added by 50.245.17.105 (talk) 15:52, 31 December 2015 (UTC)

Added cPAS section to text and Table to describe technologies I work for BGI and MGI who build and sell DNA sequencers. I have added text with appropriate references to back up claims. I have not added any text to advantages or disadvantages columns in the Table so as not make any misleading comments. (AntoBeck (talk) 04:07, 5 July 2018 (UTC))

I feel like most methods under 'Methods in development' are included in the definition High-throughput methods

I propose to make Methods in dev under High-throughput methods for more clarity.Walidou47 (talk) 13:11, 11 February 2020 (UTC)