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Myelin basic protein

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MBP
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesMBP, myelin basic protein
External IDsOMIM: 159430; MGI: 96925; HomoloGene: 1788; GeneCards: MBP; OMA:MBP - orthologs
Orthologs
SpeciesHumanMouse
Entrez

4155

17196

Ensembl

ENSG00000197971

ENSMUSG00000041607

UniProt

P02686

P04370

RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)Chr 18: 76.98 – 77.13 MbChr 18: 82.49 – 82.6 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse
Myelin_MBP
Identifiers
SymbolMyelin_MBP
PfamPF01669
InterProIPR000548
PROSITEPDOC00492
SCOP21bx2 / SCOPe / SUPFAM
OPM superfamily274
OPM protein2lug
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the nervous system. The myelin sheath is a multi-layered membrane, unique to the nervous system, that functions as an insulator to greatly increase the velocity of axonal impulse conduction.[5] MBP maintains the correct structure of myelin, interacting with the lipids in the myelin membrane.[6][7]

MBP was initially sequenced in 1971 after isolation from bovine myelin membranes.[8] MBP knockout mice called shiverer mice were subsequently developed and characterized in the early 1980s. Shiverer mice exhibit decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The human gene for MBP is on chromosome 18;[9] the protein localizes to the CNS and to various cells of the hematopoietic lineage.

The pool of MBP in the central nervous system is very diverse, with several splice variants being expressed and a large number of post-translational modifications on the protein, which include phosphorylation, methylation, deamidation, and citrullination. These forms differ by the presence or the absence of short (10 to 20 residues) peptides in various internal locations in the sequence. In general, the major form of MBP is a protein of about 18.5 Kd (170 residues).

In melanocytic cell types, MBP gene expression may be regulated by MITF.[10]

Gene expression

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well-characterized myelin basic proteins. This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.[11]

Structure

Myelin basic protein is a net-positive, membrane-associated, intrinsically disordered protein. It is basic, with an isoelectric point of 10, and is thought to associate with negatively charged phospholipid heads of the plasma membrane. Due to post-translational modifications, it can exist as various charge isomers known as C1-C6 or C8. C1-C6 each undergo ADP-ribosylation at Arg9 and Arg54; in addition, C1 is the least modified (and most positively charged), C2 is deamidated, and C3-C6 undergo a combination of phosphorylation, deamidation, and deimination. C8 is the most distinct, containing 6-11 citrullinations beyond C1, each of which decreases its positive charge and alters its ability to hydrogen bond. Additionally, they may undergo acylation of the N-terminal alanine, methylation of Arg107, and addition of an azido-GTP at Gln3.[12]

Myelin basic protein has been classified as an intrinsically disordered protein that has no stable secondary structure in solution.[13] Like most IDPs, it has a high net charge and a low mean hydrophobicity, minimizing the hydrophobic effect that drives traditional protein folding. It does contain some exceptions to normal IDP amino acid content. For example, MBP has more arginine and less glutamic acid than most IDPs. However, this is likely because those changes are necessary for MBP to be sufficiently basic and positively charged to correctly interact with the membrane.[14] Notably, MBP has been shown to adopt a more stable secondary structure on interaction with lipids.[15][16] Experiments have predicted this structure to contain beta sheet and regions of amphipathic helix.

Role in disease

Interest in MBP has centered on its role in demyelinating diseases, in particular, multiple sclerosis (MS). The target antigen of the autoimmune response in MS has not yet been identified. However, several studies have shown a role for antibodies against MBP in the pathogenesis of MS.[17] Some studies have linked a genetic predisposition to MS to the MBP gene, though a majority have not.

A "molecular mimicry" hypothesis of multiple sclerosis has been suggested, in which T cells are, in essence, confusing MBP with human herpesvirus-6. Researchers in the United States created a synthetic peptide with a sequence identical to that of an HHV-6 peptide. Elevated levels of MBP have been found in the cerebrospinal fluid of patients with HIV infections and signs of encephalopathy, and although MBP does not seem to be a sensitive diagnostic marker of HIV encephalopathy, it has been suggested that it may serve a role as a prognostic indicator of disease progression.[18] It is able to show that T cells were activated by this peptide. These activated T cells also recognized and initiated an immune response against a synthetically created peptide sequence that is identical to part of human MBP. During their research, they found that the levels of these cross-reactive T cells are significantly elevated in multiple sclerosis patients.[19]

Some research has shown that inoculating an animal with MBP to generate an MBP-specific immune response against it increases blood–brain barrier permeability. Permeability is enhanced when the animal is inoculated against non-specific proteins.[20]

A targeted immune response to MBP has been implicated in lethal rabies infection. The inoculation of MBP generates increases the permeability of the blood–brain barrier (BBB), allowing immune cells to enter the brain, the primary site of rabies virus replication. In a study of mice infected with Silver-haired bat rabies virus (SHBRV), the mortality rate of mice treated with MBP improved 20%-30% over the untreated control group. It is significant to note that healthy uninfected mice treated with MBP showed an increase in mortality rate between 0% and 40%.[21]

Interactions

Myelin basic protein has been shown to interact in vivo with proteolipid protein 1,[22][23] and in vitro with calmodulin, actin, tropomyosin, tubulin, clathrin, 2',3'-cyclic-nucleotide 3'-phosphodiesterase and multiple molecules of the immune system.[24]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000197971 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000041607 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Sakamoto Y, Kitamura K, Yoshimura K, Nishijima T, Uyemura K (March 1987). "Complete amino acid sequence of PO protein in bovine peripheral nerve myelin". The Journal of Biological Chemistry. 262 (9): 4208–14. PMID 2435734.
  6. ^ Deber CM, Reynolds SJ (April 1991). "Central nervous system myelin: structure, function, and pathology". Clinical Biochemistry. 24 (2): 113–34. doi:10.1016/0009-9120(91)90421-a. PMC 7130177. PMID 1710177.
  7. ^ Inouye H, Kirschner DA (January 1991). "Folding and function of the myelin proteins from primary sequence data". Journal of Neuroscience Research. 28 (1): 1–17. doi:10.1002/jnr.490280102. PMID 1710279. S2CID 8598890.
  8. ^ Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P (September 1971). "Basic A1 protein of the myelin membrane. The complete amino acid sequence". The Journal of Biological Chemistry. 246 (18): 5770–84. PMID 5096093.
  9. ^ Saxe DF, Takahashi N, Hood L, Simon MI (1985). "Localization of the human myelin basic protein gene (MBP) to region 18q22----qter by in situ hybridization". Cytogenetics and Cell Genetics. 39 (4): 246–9. doi:10.1159/000132152. PMID 2414074.
  10. ^ Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (December 2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell & Melanoma Research. 21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID 19067971. S2CID 24698373.
  11. ^ "Entrez Gene: myelin basic protein".
  12. ^ Boggs JM (September 2006). "Myelin basic protein: a multifunctional protein". Cellular and Molecular Life Sciences. 63 (17): 1945–61. doi:10.1007/s00018-006-6094-7. PMID 16794783. S2CID 1076002.
  13. ^ Anderson TR, Slotkin TA (August 1975). "Maturation of the adrenal medulla--IV. Effects of morphine". Biochemical Pharmacology. 24 (16): 1469–74. doi:10.1016/0006-2952(75)90020-9. PMID 7.
  14. ^ Harauz G, Ishiyama N, Hill CM, Bates IR, Libich DS, Farès C (May 2004). "Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis". Micron. 35 (7): 503–42. doi:10.1016/s0968-4328(04)00096-4. PMID 15219899.
  15. ^ Moroi K, Sato T (August 1975). "Comparison between procaine and isocarboxazid metabolism in vitro by a liver microsomal amidase-esterase". Biochemical Pharmacology. 24 (16): 1517–21. doi:10.1016/0006-2952(75)90029-5. PMID 8.
  16. ^ Marniemi J, Parkki MG (September 1975). "Radiochemical assay of glutathione S-epoxide transferase and its enhancement by phenobarbital in rat liver in vivo". Biochemical Pharmacology. 24 (17): 1569–72. doi:10.1016/0006-2952(75)90080-5. PMID 9.
  17. ^ Berger T, Rubner P, Schautzer F, Egg R, Ulmer H, Mayringer I, Dilitz E, Deisenhammer F, Reindl M (July 2003). "Antimyelin antibodies as a predictor of clinically definite multiple sclerosis after a first demyelinating event". The New England Journal of Medicine. 349 (2): 139–45. doi:10.1056/NEJMoa022328. PMID 12853586.
  18. ^ Pfister HW, Einhäupl KM, Wick M, Fateh-Moghadam A, Huber M, Schielke E, Goebel FD, Matuschke A, Heinrich B, Bogner JR (July 1989). "Myelin basic protein in the cerebrospinal fluid of patients infected with HIV". Journal of Neurology. 236 (5): 288–91. doi:10.1007/bf00314458. PMID 2474637. S2CID 12178626.
  19. ^ Tejada-Simon MV, Zang YC, Hong J, Rivera VM, Zhang JZ (February 2003). "Cross-reactivity with myelin basic protein and human herpesvirus-6 in multiple sclerosis". Annals of Neurology. 53 (2): 189–97. doi:10.1002/ana.10425. PMID 12557285. S2CID 43317994.
  20. ^ Namer IJ, Steibel J, Poulet P, Armspach JP, Mohr M, Mauss Y, Chambron J (February 1993). "Blood-brain barrier breakdown in MBP-specific T cell induced experimental allergic encephalomyelitis. A quantitative in vivo MRI study". Brain. 116 ( Pt 1) (1): 147–59. doi:10.1093/brain/116.1.147. PMID 7680933.
  21. ^ Roy A, Hooper DC (August 2007). "Lethal silver-haired bat rabies virus infection can be prevented by opening the blood-brain barrier". Journal of Virology. 81 (15): 7993–8. doi:10.1128/JVI.00710-07. PMC 1951307. PMID 17507463.
  22. ^ Wood DD, Vella GJ, Moscarello MA (October 1984). "Interaction between human myelin basic protein and lipophilin". Neurochemical Research. 9 (10): 1523–31. doi:10.1007/BF00964678. PMID 6083474. S2CID 9751765.
  23. ^ Edwards AM, Ross NW, Ulmer JB, Braun PE (January 1989). "Interaction of myelin basic protein and proteolipid protein". Journal of Neuroscience Research. 22 (1): 97–102. doi:10.1002/jnr.490220113. PMID 2467009. S2CID 33666906.
  24. ^ Harauz G, Ishiyama N, Hill CM, Bates IR, Libich DS, Farès C (2004). "Myelin basic protein-diverse conformational states of an intrinsically unstructured protein and its roles in myelin assembly and multiple sclerosis". Micron. 35 (7): 503–42. doi:10.1016/j.micron.2004.04.005. PMID 15219899.

Further reading

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

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