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Buffered charcoal yeast extract agar

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Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila.[1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis,[2] Francisella and Nocardia.

The growth medium is prepared from the following components:

Its selectivity stems from the inclusion of activated charcoal. Without it, the medium itself is able to support the growth of a variety of organisms.

References

  1. ^ "Archived copy" (PDF). Archived from the original (PDF) on 2012-06-12. Retrieved 2012-05-11.{{cite web}}: CS1 maint: archived copy as title (link)
  2. ^ Penland RL, Wilhelmus KR (October 1998). "Laboratory diagnosis of Acanthamoeba keratitis using buffered charcoal-yeast extract agar". Am. J. Ophthalmol. 126 (4): 590–2. doi:10.1016/S0002-9394(98)00125-1. PMID 9780107.