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Freezing point depression osmometer

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This article is about the theory of freezing point depression osmometry applied on the osmometer technique. For the phenomenon in pure fluids, see supercooling.
Freezing point depression osmometer
Freezing point depression
ClassificationOsmolarity, Analytical chemistry
AnalytesA phenomenon caused by solutes that can measure with this technique

The freezing point depression osmometer is an osmometer that is used in determining a solution's osmotic concentration as its osmotically active aspects depress its freezing point. Osmometry further involves other techniques, including membrane osmometry, which determines the osmotic pressure of solutions, and vapor pressure osmometry, which assesses the concentration of particles that minimize a solution's vapor pressure and melting, as well as the freezing points of aqueous solutions.[1]

Freezing point depression osmometry is commonly used in distinct contexts. In the past, it has been used to assess the osmotic strength of colloids and solutions. The osmometer uses the solution's freezing point depression to establish its strength. It is used to determine the level of osmotically appropriate body fluid in various chemicals dissolved in the blood using the relationship in which a mole of dissolved substance reduces the freezing point of water by 1.86 °C (35.35 °F). Being efficient, the freezing point depression osmometer is used in various medical practices, including pharmaceutical manufacturing, quality control laboratories, and clinical chemistry.

Method

Freezing point depression osmometers are utilized to determine a solution's osmotic strength. It is the most preferred method that is applicable to various activities in the medical field. It is used in assessing the osmotic strength of colloids as well as solutions.[2]

The freezing point depression osmometer operates by using the solution's freezing point to determine the concentration of the solution. It uses a nanoliter nanometer, a device that facilitates the establishment of the solution's melting and freezing points. Calibration, loading, deep freezing, and determination are the four separate procedures involved in determining the freezing and melting points. The concentration of the solution can be determined by knowing the number of particles present in it, which can be done by determining the freezing point of the solution.

When particles are dissolved in a solution, their freezing point is lowered compared to that of the original solvent. A further increase in the solute decreases the freezing point even further. The freezing point depression osmometer uses the solution's freezing point to establish its concentration.[3] The freezing point depression osmometer is calibrated using standards that are within the solution's osmolality range.

Freezing point depression
Freezing point
Principle graph of chemical potential against temperature explaining freezing point depression and boiling point elevation
ManufacturersTomas er
Other techniques
RelatedMelting-point depression, Boiling-point elevation

History of use

The use of osmometers began in the late nineteenth century after van’t Hoff won a Nobel Prize for his research and discovery that the relationship between the osmotic pressure of dilute colloid solutions and concentration was consistent with the ideal gas law.[4] Since then, osmometers have been used to measure the osmotic strength of a diluted solution at different levels of concentration.

The historical use of the method has been validated by Guerrero et al.[5] in an analytical study, in which they tested the urine osmolality of 1,991 dogs. The study discloses the past achievements of professionals who, using the approach, established intervals and the impacts of sex, age, and reproductive status. The historical use of the method is further disclosed by Hale et al.[6] who elaborate on its advantages over other conventional concentration osmometers, which rely on the osmotic pressure profile.

Current usage in medical fields

Freezing point depression osmometers are applied in various areas of the medical field. The approach is used in determining the colloidal aspects of solutions. [7] According to Hale et al., the freezing point depression osmometer has certain advantages over other conventional approaches, which may explain its increased application in the medical sector. In the present day, the method is applied, among other areas, in measuring osmolarity in lens care solutions as well as eye drops, promoting eye health.[8] It is further used in clinical chemistry, pharmaceutical, and quality control laboratories, where it facilitates different processes. As compared to the other methods, the freezing point depression osmometer has a high level of precision and accuracy, making its application in clinical practices safe. It is applied in various processes that involve the manufacturing of drugs.[9] Urine osmolality is also used to measure urine concentration accurately and thus determine renal function and body fluid homeostasis.

Evaluation of its use

Osmometry is widely used in pharmaceuticals, quality control laboratories, and clinical chemistry to measure the osmolality in aqueous solutions accurately. The freezing point depression osmometer is a preferred method in the medical sector due to its high precision. It is commonly used in medical clinics to assist with various pharmaceutical practices,[10] including the development of lens care solutions and eye drops that promote eye health. The method has been successful in the past and continues to be effective today in different medical settings.

See also

Further reading

  • Skoog, D.A.; West, D.M.; Holler, F.J. Fundamentals of Analytical Chemistry New York: Saunders College Publishing, 5th Edition, 1988.
  • Bard, A.J.; Faulkner, L.R. Electrochemical Methods: Fundamentals and Applications. New York: John Wiley & Sons, 2nd Edition, 2000.
  • Bettencourt da Silva, R; Bulska, E; Godlewska-Zylkiewicz, B; Hedrich, M; Majcen, N; Magnusson, B; Marincic, S; Papadakis, I; Patriarca, M; Vassileva, E; Taylor, P; Analytical measurement: measurement uncertainty and statistics, 2012, ISBN 978-92-79-23070-7.

References

  1. ^ Guerrero, Samantha; Pastor, Josep; Tvarijonaviciute, Asta; Cerón, José Joaquín; Balestra, Graziano; Caldin, Marco (2017-08-14). "Analytical validation and reference intervals for freezing point depression osmometer measurements of urine osmolality in dogs". Journal of Veterinary Diagnostic Investigation. 29 (6): 791–796. doi:10.1177/1040638717726114. ISSN 1040-6387. PMID 28803509.
  2. ^ SUZUKI, Masahiko; ITO, Kiyoko; FUSHIMI, Chigusa; KONDO, Tamotsu (1993). "A Study of Cyclodextrin Complex Formation by a Freezing Point Depression Method". Chemical & Pharmaceutical Bulletin. 41 (5): 942–945. doi:10.1248/cpb.41.942. ISSN 0009-2363.
  3. ^ SUZUKI, Masahiko; ITO, Kiyoko; FUSHIMI, Chigusa; KONDO, Tamotsu (1993). "A Study of Cyclodextrin Complex Formation by a Freezing Point Depression Method". Chemical & Pharmaceutical Bulletin. 41 (5): 942–945. doi:10.1248/cpb.41.942. ISSN 0009-2363.
  4. ^ Hale, Christopher S.; McBride, Devin W.; Batarseh, Ramsey; Hughey, Jordan; Vang, Kevin; Rodgers, V. G. J. (March 2019). "Development and applications of a concentrating membrane osmometer for colloid solutions". Review of Scientific Instruments. 90 (3): 034102. Bibcode:2019RScI...90c4102H. doi:10.1063/1.5065512. ISSN 0034-6748. PMID 30927796.
  5. ^ Guerrero, Samantha; Pastor, Josep; Tvarijonaviciute, Asta; Cerón, José Joaquín; Balestra, Graziano; Caldin, Marco (2017-08-14). "Analytical validation and reference intervals for freezing point depression osmometer measurements of urine osmolality in dogs". Journal of Veterinary Diagnostic Investigation. 29 (6): 791–796. doi:10.1177/1040638717726114. ISSN 1040-6387. PMID 28803509.
  6. ^ Hale, Christopher S.; McBride, Devin W.; Batarseh, Ramsey; Hughey, Jordan; Vang, Kevin; Rodgers, V. G. J. (March 2019). "Development and applications of a concentrating membrane osmometer for colloid solutions". Review of Scientific Instruments. 90 (3): 034102. Bibcode:2019RScI...90c4102H. doi:10.1063/1.5065512. ISSN 0034-6748. PMID 30927796.
  7. ^ Hale, Christopher S.; McBride, Devin W.; Batarseh, Ramsey; Hughey, Jordan; Vang, Kevin; Rodgers, V. G. J. (March 2019). "Development and applications of a concentrating membrane osmometer for colloid solutions". Review of Scientific Instruments. 90 (3): 034102. Bibcode:2019RScI...90c4102H. doi:10.1063/1.5065512. ISSN 0034-6748. PMID 30927796.
  8. ^ Pena-Verdeal, Hugo; García-Resúa, Carlos; Miñones, Mercedes; Giraldez, Maria J.; Yebra-Pimentel, Eva (September 2015). "Accuracy of a Freezing Point Depression Technique Osmometer". Optometry and Vision Science. 92 (9): e273 – e283. doi:10.1097/opx.0000000000000669. ISSN 1040-5488. PMID 26164315.
  9. ^ Nolfi, Jerry; Caffery, Barbara (May 2017). "Randomized comparison of in vivo performance of two point-of-care tear film osmometers". Clinical Ophthalmology. 11: 945–950. doi:10.2147/opth.s135068. ISSN 1177-5483. PMC 5449174. PMID 28579744.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  10. ^ SUZUKI, Masahiko; ITO, Kiyoko; FUSHIMI, Chigusa; KONDO, Tamotsu (1993). "A Study of Cyclodextrin Complex Formation by a Freezing Point Depression Method". Chemical & Pharmaceutical Bulletin. 41 (5): 942–945. doi:10.1248/cpb.41.942. ISSN 0009-2363.
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