Double distilled water
Double distilled water (abbreviated "ddH2O" or "Bidest. water") is prepared by double distillation of deionized water. It is used, among other things, when single distillation does not lead to sufficiently pure water for some applications in biochemistry.
Bidest. water is used when pure, sterile water is essential. Whereas distilled water is enough for most chemical reactions, a molecular biologist attempting to create sterile, enzyme-free media might use bidest. water to ensure sterility.
Modern molecular biology labs typically use water purification systems such as Milli-Q (by Millipore) to create ultra-pure water.
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