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ATP test

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ATP Testing Method

ATP testing is the process of rapidly measuring active microorganisms through detection of a molecule called Adenosine Triphosphate, or ATP.

ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally-occurring firefly enzyme Luciferase using a Luminometer. The amount of light produced is directly proportional to the amount of biological energy present in the sample[1].

If there are microorganisms present, there is ATP!

ATP tests can be used to:

  • Control biological treatment reactors
  • Guide biocide dosing programs
  • Determine drinking water cleanliness
  • Manage fermentation processes
  • Assess soil activity
  • Determine corrosion / deposit process type
  • Measure equipment or product sanitation

1st Generation vs 2nd Generation

1st Generation ATP test formats are derived from hygiene monitoring uses where samples are relatively free of interferences. 2nd Generation tests are specifically designed for water, wastewater and industrial applications where, for the most part, samples contain a variety of components that can interfere with the ATP assay[2].

References