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Talk:Fluorescence resonance energy transfer

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This is an old revision of this page, as edited by 128.6.78.114 (talk) at 21:09, 22 December 2005 (Kappa squared comment is wrong.). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Hi

I think that is would be more illustrative to have a spectrum that shows no FRET also. With the present spectrum I think it is difficult to see what distinguishes a FRET measurement from just taking an ordinary emission spectrum.


I think it would also be usefull to label the FRET graph.

I was thinking about creating a page for BRET (Bioluminescence Resonance Energy Transfer), but I am hesitating: It could just be a section in FRET. It's basically the same thing as FRET except that the photon emission from the donor is biochemically catalysed (by Luciferase-type donor]]) instead of being the result of exciting the donor with an external source. Result: You don't get background noise from the fluorescence of the donor in the results. I am too new to wikipedia to decide :) Looie 16:26, 16 November 2005 (UTC)[reply]

I resolved my moral dillema and simply added a small paragraph to the current page. Looie 04:11, 17 November 2005 (UTC)[reply]

Kappa squared comment is wrong.

Kappa squared only equals 2/3 when BOTH dyes are isotropically averaged. This should be obvious to anybody who considers the dipole electric field pattern in Three dimensional space. I have corrected the misstatement but I have not included a proper discussion of Kappa Squared. --128.6.78.114 21:09, 22 December 2005 (UTC)[reply]

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