This round is the first in which Conference National teams join those from lower reaches of the National League System. Matches took place on the 12th December. The fixture between Maidstone United and Histon was postponed in December due to a waterlogged pitch and rearranged for 15th December. This match, however, did not go ahead because of a frozen pitch and neither did the third attempt at playing this fixture on 22nd December. The match was then scheduled to take place on 5th January, but this was also postponed due to bad weather. The match was then scheduled to take place on 12th January, but was subsequently postponed once more.[1][2][3] The game was eventually played on 19th January.
Matches were to take place on Saturday, 9 January 2010, but the bad weather that engulfed the country meant that all were postponed and rescheduled provisionally for between the 11th and 13th January.[4] Most of the games were further postponed to between the 18th and 20th January.