Insertion sequence

Insertion sequences (also known as insertion elements, ISs, insertion sequence elements, or IS elements) are short DNA sequences that act as simple transposable elements; insertion sequences only code for a transposition enzyme (a transposase). The transposase coding region in an insertion sequence is usually flanked by inverted repeats. Insertion sequences are generally around 700 to 2500 bp in length. Generally, a particular insertion sequence is named according to the form ISn, where n is a number (IS1, IS2, etc.). Although insertion sequences are usually discussed in the context of prokaryotic genomes, eukaryotic insertion sequences also exist.

In addition to occurring autonomously, insertion sequences may also occur as parts of composite transposons; in a composite transposon (also known as a "complex transposon" or sometimes simply referred to as a "transposon"), two insertion sequences flank one or more non-transposition genes.

References

• Campbell, Neil A. and Reece, Jane B. (2002). Biology (6th ed.), pp. 345-346. San Francisco: Benjamin Cummings. ISBN 0-8053-6624-5.
• Mahillon, Jacques and Chandler, Michael (1998). "Insertion sequences". Microbiology and Molecular Biology Reviews 62 (3), 725-774. Retrieved January 11, 2006.
• Prescott, Lansing M.; Harley, John P.; and Klein, Donald A. (2002). Microbiology (5th ed.), pp. 298-299. New York: McGraw-Hill. ISBN 0-07-232041-9.
• Shuler, Michael L. and Kargi, Fikret (2002). Bioprocess Engineering: Basic Concepts (2nd ed.), pp. 220. Upper Saddle River, NJ: Prentice Hall PTR. ISBN 0-13-081908-5.

External link

• IS Finder