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MiR-132

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This is an old revision of this page, as edited by A.j.perrin (talk | contribs) at 14:31, 4 November 2010 (→Role in Neuronal Cells). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

mir-132
Identifiers
Symbolmir-132
RfamRF00662
miRBase familyMIPF0000065
Other data
RNA typemicroRNA
Domain(s)Eukaryota;
PDB structuresPDBe

In molecular biology mir-132 microRNA is a short RNA molecule. MicroRNAs function to regulate the expression levels of other genes by several mechanisms, generally reducing protein levels though the cleavage of mRNAs or the repression of their translation. Several targets for miR-132 have been described, including mediators of inflammation and synaptic transmission.

Expression

miR-132 arises from the miR-212/132 cluster located in the intron of a non-coding gene on mouse chromosome 11. The transcription of this cluster was found to be enhanced by the transcription factor CREB (cAMP-response element binding protein). In neuronal cells BDNF (brain derived neurotrophic factor) is known to induce the transcription of this cluster; the pathway is thought to involve the BDNF-mediated activation of ERK1/2, which in turn activates MSK, another kinase enzyme. MSK-mediated phosphorylation of a serine residue on CREB may then enhance production of miR-132. MSK knockout mice still produce miR-132 in response to BDNF, but at a significantly lower level, indicating that there may be an alternative pathway operating. Activators of CREB phosphorylation, for instance forskolin, can also enhance miR-132 production in vitro. Neuronal stimulation is thought to increase the levels of miR-132 in the brain. One example is in the suprachiasmatic nucleus, where miR-132 is thought be involved in resetting the circadian clock in response to light. miR-132 levels are increased post-seizure, which strongly suggests a link with neuronal activation. Inflammatory mediators such as Lipopolysaccharide (LPS) are also implicated in inducing miR-132 expression.

Role in Neuronal Cells

miR-132 is enriched in neuronal cells. Recognition elements for this miRNA have been identified in a number of cellular mRNAs. One such mRNA is that of p250GAP, a GTPase activating protein linked to neuronal differentiation. miR-132 and its recognition site on p250GAP mRNA are highly conserved among vertebrates, and their interaction is suspected to have a role in vertebrate neurogenesis. By decreasing the levels of p250GAP, miR-132 promotes neuronal outgrowth and sprouting.

Another target for miR-132 is MeCP2, whose mRNA is expressed as a 'long' variant in neuronal cells. This variant contains a recognition element for miR-132 in its extended 3'UTR. miRNA-132 may be involved in a homeostatic mechanism that regulates MeCP2 levels in the brain. MeCP2 increases the levels of BDNF in the brain, which in turn will increase transcription from the miR-212/132 cluster. An rise in miRNA-132 level will then decrease the levels of MeCP2 and restore the balance. Failure to regulate MeCP2 levels is connected to neurological disorders including Rett syndrome.

The role of miR-132 in synaptic function is currently being studied. A BDNF-related increase in miR-132 is thought to bring about an increse in post-synaptic protein levels. miR-132 has been found to associate with Fragile X Mental Retardation Protein FMRP, and may be involved in the selection of mRNAs, including those regulating synaptic function, to undergo translational suppression via an FMRP-dependent mechansim.

See also

Further reading

  1. ^ Anand S, Majeti BK, Acevedo LM, Murphy EA, Mukthavaram R, Scheppke L, Huang M, Shields DJ, Lindquist JN, Lapinski PE, King PD, Weis SM, Cheresh DA (2010). "MicroRNA-132-mediated loss of p120RasGAP activates the endothelium to facilitate pathological angiogenesis". Nat Med. 16 (8): 909–14. PMID 20676106.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  2. ^ Lagos D, Pollara G, Henderson S, Gratrix F, Fabani M, Milne RS, Gotch F, Boshoff C (2010). "miR-132 regulates antiviral innate immunity through suppression of the p300 transcriptional co-activator". Nat Cell Biol. 12 (5): 513–9. PMID 20418869.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  3. ^ Edbauer D, Neilson JR, Foster KA, Wang CF, Seeburg DP, Batterton MN, Tada T, Dolan BM, Sharp PA, Sheng M (2010). "Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132". Neuron. 65 (3): 373–84. PMID 20159450.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  4. ^ O'Neill LA (2009). "Boosting the brain's ability to block inflammation via microRNA-132". Immunity. 31 (6): 854–5. PMID 20064444.
  5. ^ Shaked I, Meerson A, Wolf Y, Avni R, Greenberg D, Gilboa-Geffen A, Soreq H (2009). "MicroRNA-132 potentiates cholinergic anti-inflammatory signaling by targeting acetylcholinesterase". Immunity. 31 (6): 965–73. PMID 20005135.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  6. ^ Kawashima H, Numakawa T, Kumamaru E, Adachi N, Mizuno H, Ninomiya M, Kunugi H, Hashido K (2010). "Glucocorticoid attenuates brain-derived neurotrophic factor-dependent upregulation of glutamate receptors via the suppression of microRNA-132 expression". Neuroscience. 165 (4): 1301–11. PMID 19958814.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  7. ^ Strum JC, Johnson JH, Ward J, Xie H, Feild J, Hester A, Alford A, Waters KM (2009). "MicroRNA 132 regulates nutritional stress-induced chemokine production through repression of SirT1". Mol Endocrinol. 23 (11): 1876–84. PMID 19819989.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  8. ^ Nudelman AS, DiRocco DP, Lambert TJ, Garelick MG, Le J, Nathanson NM, Storm DR (2010). "Neuronal activity rapidly induces transcription of the CREB-regulated microRNA-132, in vivo". Hippocampus. 20 (4): 492–8. PMID 19557767.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  9. ^ Remenyi J, Hunter CJ, Cole C, Ando H, Impey S, Monk CE, Martin KJ, Barton GJ, Hutvagner G, Arthur JS (2010). "Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins". Biochem J. 428 (2): 281–91. PMID 20307261.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  10. ^ Vo N, Klein ME, Varlamova O, Keller DM, Yamamoto T, Goodman RH, Impey S (2005). "A cAMP-response element binding protein-induced microRNA regulates neuronal morphogenesis". Proc Natl Acad Sci USA. 102 (45): 16426–31. PMID 16260724.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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