K562 cells
K562 cells were the first human immortalised myelogenous leukaemia line to be established. K562 cells are of the erythroleukemia type, and the line is derived from a 53 year old female CML patient in blast crisis.[1] [2] The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes[3] and erythrocytes.[4]
In culture they exhibit much less clumping than many other suspension lines, presumably due to the downregulation of surface adhesion molecules by bcr:abl.[citation needed] K562s can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes[5] and are easily killed by natural killer cells[6] as they lack the MHC complex required to inhibit NK activity.[2] They also lack any trace of Epstein-Barr virus and other herpesviruses. In addition to the Philadelphia chromosome they also exhibit a second reciprocal translocation between the long arm of chromosome 15 with chromosome 17.[1]
Two sub-lines are available which express MHC class-I A2[7] and A3.[8]
The K562 cell line can be obtained from a number of biological resource centres such as the European Collection of Cell Cultures (ECACC) which is part of the Health Protection Agency Culture Collections. K562 data, such as growth curves, time lapse videos of growth, images and subculture routine information are available from ECACC.
References
- ^ a b Lozzio, C.B.; Lozzio, B.B. (1975), "Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome", Blood, 45 (3): 321–334, PMID 163658
- ^ a b Drexler, H.G. (2000), The Leukemia-Lymphoma Cell Line Factsbook, San Diego: Academic Press
- ^ Klein, E.; Ben-Bassat, H.; Neumann, H.; Ralph, P.; Zeuthen, J.; Polliack, A.; Vánky, F. (1976), "Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia", International Journal of Cancer, 18 (4): 421–431, doi:10.1002/ijc.2910180405, PMID 789258
- ^ Andersson, L.C.; Nilsson, K.; Gahmberg, C.G. (1979), "K562 - A human erythroleukemic cell line", International Journal of Cancer, 23 (2): 143–147, doi:10.1002/ijc.2910230202, PMID 367973
- ^ Lozzio, B.B.; Lozzio, C.B.; Bamberger, E.G.; Feliu, A.S. (1981), "A multipotential leukemia cell line (K-562) of human origin", Proceedings of the Society for Experimental Biology and Medicine, 166: 546–550, PMID 7194480
- ^ Lozzio, B.B.; Lozzio, C.B. (1979), "Properties and usefulness of the original K-562 human myelogenous leukemia cell line", Leukemia Research, 3 (6): 363–370, PMID 95026
- ^ Britten, C.M.; Meyer, R.G.; Kreer, T.; Drexler, I.; Wölfel, T.; Herr, W. (2002), "The use of HLA-A*0201-transfected K562 as standard antigen-presenting cells for CD8(+) T lymphocytes in IFN-gamma ELISPOT assays", Journal of Immunological Methods, 259 (1–2): 95–110, PMID 11730845
- ^ Clark, R.E.; Dodi, I.A.; Hill, S.C.; Lill, J.R.; Aubert, G.; Macintyre, A.R.; Rojas, J.; Bourdon, A.; Bonner, P.L.; Wang, L.; Christmas, S.E.; Travers, P.J.; Creaser, C.S.; Rees, R.C.; Madrigal, J.A. (2001), "Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein", Blood, 98 (10): 2887–2893, PMID 11698267
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