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Talk:Risk of Missed PRRS PCR Detection

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This is an old revision of this page, as edited by 570ceh (talk | contribs) at 16:52, 8 November 2011 (→PCR questions: new section). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Under Hazard to detection, did you mean for the indentation to occur that way or was that by accident? -Vic 570vca (talk) 17:45, 3 November 2011 (UTC)[reply]

I went ahead and removed the bullet points and added arrows and spaces instead to try to make it a bit more readable. However, I'm not sure I'm happy with how it turned out. Since you said you wanted a flow chart type effect, and since there appears to be only one line of succession in the "flowchart", would just a numbered list would be better?? Let me know what you think. -Vic 570vca (talk) 13:06, 4 November 2011 (UTC)[reply]

Maybe a little info on the implications of PRRS would be helpful in the opening paragraph. Also, I really liked your pathway format. Do you mind if I borrow your idea. - Dan (570ddt (talk) 01:30, 5 November 2011 (UTC))[reply]

Cleaned up the references and some minor spelling errors. Added a sentence to clarify what a false-negative result is in the context of this article. -Vic 570vca (talk) 18:26, 6 November 2011 (UTC)[reply]

Hi Wayne! This is nicely written. One item I would recommend: In the discussion of virus mutation and evolution, I would add a few sentences about the role of genetic recombination in increasing diversity. This appears to be a major contributor to the speed of evolution and there are plausible mechanisms and computational models. I do understand why you are choosing to focus on single nucleotide substitution since this may be more difficult to detect. Cheers! 570mpp (talk) 17:45, 7 November 2011 (UTC)[reply]

PCR questions

Hi! I have been doing some work with using real time PCR for detection of Salmonella in environmental samples, so I know just enough to be dangerous. Can you explain to me why working with the amplified PCR product from nested PCR could produce false positives? I am not sure if it is necessary to add that to your page, but I would like to know. Also, it was my understanding that the Taqman probes used for real-time PCR bind to double stranded DNA which results from other steps in the amplification process, but that the probes were not specific to a certain sequence themselves. I am a geologist trying to learn microbiology without a strong grasp of the fundementals, so forgive me if this is a stupid question! 570ceh (talk) 16:52, 8 November 2011 (UTC)[reply]

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