List of RNA structure prediction software
This list of RNA structure prediction software is a compilation of software tools and web portals used for RNA structure prediction.
Single sequence secondary structure prediction
Name | Description | Knots [Note 1] |
Links | References |
---|---|---|---|---|
CentroidFold | Secondary structure prediction based on generalized centroid estimator | no | sourcecode webserver | [1] |
CentroidHomfold | Secondary structure prediction by using homologous sequence information | no | sourcecode webserver | [2] |
CONTRAfold | Secondary structure prediction method based on conditional log-linear models (CLLMs), a flexible class of probabilistic models which generalize upon SCFGs by using discriminative training and feature-rich scoring. | no | sourcecode webserver | [3] |
CyloFold | Secondary structure prediction method based on placement of helices allowing complex pseudoknots. | yes | webserver | [4] |
KineFold | Folding kinetics of RNA sequences including pseudoknots by including an implementation of the partition function for knots. | yes | linuxbinary, webserver | [5][6] |
Mfold | MFE (Minimum Free Energy) RNA structure prediction algorithm. | no | sourcecode, webserver | [7] |
Pknots | A dynamic programming algorithm for optimal RNA pseudoknot prediction using the nearest neighbour energy model. | yes | sourcecode | [8] |
PknotsRG | A dynamic programming algorithm for the prediction of a restricted class of RNA pseudoknots. | yes | sourcecode, webserver | [9] |
RNA123 | Secondary structure prediction via thermodynamic-based folding algorithms and novel structure-based sequence alignment specific for RNA. | yes | webserver | |
RNAfold | MFE RNA structure prediction algorithm. Includes an implementation of the partition function for computing basepair probabilities and circular RNA folding. | no | sourcecode, webserver | |
RNAshapes | MFE RNA structure prediction based on abstract shapes. Shape abstraction retains adjacency and nesting of structural features, but disregards helix lengths, thus reduces the number of suboptimal solutions without losing significant information. Furthermore, shapes represent classes of structures for which probabilities based on Boltzmann-weighted energies can be computed. | no | source & binaries, webserver | [14][15] |
RNAstructure | A program to predict lowest free energy structures and base pair probabilities for RNA or DNA sequences. Programs are also available to predict Maximum Expected Accuracy structures and these can include pseudoknots. Structure prediction can be constrained using experimental data, including SHAPE, enzymatic cleavage, and chemical modification accessibility. Graphical user interfaces are available for Windows and for Mac OS-X/Linux. Programs are also available for use with Unix-style text interfaces. Additionally, a C++ class library is available. | yes | source & binaries | |
Sfold | Statistical sampling of all possible structures. The sampling is weighted by partition function probabilities. | no | webserver | [18][19][20][21] |
UNAFold | The UNAFold software package is an integrated collection of programs that simulate folding, hybridization, and melting pathways for one or two single-stranded nucleic acid sequences. | no | sourcecode | [22] |
Crumple | Crumple is simple, cleanly written software for producing the full set of possible secondary structures for a single sequence, given optional constraints. | no | sourcecode | [23] |
Sliding Windows & Assembly | Sliding windows and assembly is a tool chain for folding long series of similar hairpins. | no | sourcecode | [23] |
|
Single sequence tertiary structure prediction
Name | Description | Knots [Note 1] |
Links | References |
---|---|---|---|---|
BARNACLE | A Python library for the probabilistic sampling of RNA structures that are compatible with a given nucleotide sequence and that are RNA-like on a local length scale. | yes | sourcecode | [24] |
FARNA | Automated de novo prediction of native-like RNA tertiary structures . | yes | sourcecode | [25] |
iFoldRNA | three-dimensional RNA structure prediction and folding | yes | webserver | [26] |
MC-Fold MC-Sym Pipeline | Thermodynamics and Nucleotide cyclic motifs for RNA structure prediction algorithm. 2D and 3D structures. | yes | sourcecode, webserver | [27] |
NAST | Coarse-grained modeling of large RNA molecules with knowledge-based potentials and structural filters | ? | sourcecode | [28] |
RNA123 | An integrated platform for de novo and homology modeling of RNA 3D structures, where coordinate file input, sequence editing, sequence alignment, structure prediction and analysis features are all accessed from a single intuitive graphical user interface. | yes | webserver | |
|
Comparative methods
The single sequence methods mentioned above have a difficult job detecting a small sample of reasonable secondary structures from a large space of possible structures. A good way to reduce the size of the space is to use evolutionary approaches. Structures that have been conserved by evolution are far more likely to be the functional form. The methods below use this approach.
Name | Description | Number of sequences [Note 1] |
Alignment [Note 2] |
Structure [Note 3] |
Knots [Note 4] |
Link | References |
---|---|---|---|---|---|---|---|
Carnac | Comparative analysis combined with MFE folding. | any | no | yes | no | sourcecode, webserver | [29][30] |
CentroidAlifold | Common secondary structure prediction based on generalized centroid estimator | any | yes | no | no | sourcecode webserver | [31] |
CentroidAlign | Fast and accurate multiple aligner for RNA sequences | any | yes | no | no | sourcecode | [32] |
CMfinder | an expectation maximization algorithm using covariance models for motif description. Uses heuristics for effective motif search, and a Bayesian framework for structure prediction combining folding energy and sequence covariation. | yes | yes | no | sourcecode, webserver, website | [33] | |
CONSAN | implements a pinned Sankoff algorithm for simultaneous pairwise RNA alignment and consensus structure prediction. | 2 | yes | yes | no | sourcecode | [34] |
Dynalign | an algorithm that improves the accuracy of structure prediction by combining free energy minimization and comparative sequence analysis to find a low free energy structure common to two sequences without requiring any sequence identity. | 2 | yes | yes | no | sourcecode | [35][36][37] |
FoldalignM | A multiple RNA structural RNA alignment method, to a large extend based on the PMcomp program. | any | yes | yes | no | sourcecode | [38] |
GraphClust | Fast RNA structural clustering method of local RNA secondary structures. Predicted clusters are refined using LocARNA and CMsearch. Due to the linear time complexity for clustering it is possible to analyse large RNA datasets. | any | yes | yes | no | sourcecode | [39] |
KNetFold | Computes a consensus RNA secondary structure from an RNA sequence alignment based on machine learning. | any | input | yes | yes | linuxbinary, webserver | [40] |
LARA | Produce a global fold and alignment of ncRNA families using integer linear programming and Lagrangian relaxation. | any | yes | yes | no | sourcecode | [41] |
LocaRNA | LocaRNA is the successor of PMcomp with an improved time complexity. It is a variant of Sankoff's algorithm for simultaneous folding and alignment, which takes as input pre-computed base pair probability matrices from McCaskill's algorithm as produced by RNAfold -p. Thus the method can also be viewed as way to compare base pair probability matrices. | any | yes | yes | no | sourcecode | [42] |
MASTR | A sampling approach using Markov chain Monte Carlo in a simulated annealing framework, where both structure and alignment is optimized by making small local changes. The score combines the log-likelihood of the alignment, a covariation term and the basepair probabilities. | any | yes | yes | no | sourcecode | [43][44] |
Multilign | This method uses multiple Dynalign calculations to find a low free energy structure common to any number of sequences. It does not require any sequence identity. | any | yes | yes | no | sourcecode | [45] |
Murlet | a multiple alignment tool for RNA sequences using iterative alignment based on Sankoff's algorithm with sharply reduced computational time and memory. | any | yes | yes | no | webserver | [46] |
MXSCARNA | a multiple alignment tool for RNA sequences using progressive alignment based on pairwise structural alignment algorithm of SCARNA. | any | yes | yes | no | webserver sourcecode | [47] |
PARTS | A method for joint prediction of alignment and common secondary structures of two RNA sequences using a probabilistic model based on pseudo free energies obtained from precomputed base pairing and alignment probabilities. | 2 | yes | yes | no | sourcecode | [48] |
Pfold | Folds alignments using a SCFG trained on rRNA alignments. | input | yes | no | webserver | [49][50] | |
PETfold | Formally integrates both the energy-based and evolution-based approaches in one model to predict the folding of multiple aligned RNA sequences by a maximum expected accuracy scoring. The structural probabilities are calculated by RNAfold and Pfold. | any | input | yes | no | sourcecode | [51] |
PMcomp/PMmulti | PMcomp is a variant of Sankoff's algorithm for simultaneous folding and alignment, which takes as input pre-computed base pair probability matrices from McCaskill's algorithm as produced by RNAfold -p. Thus the method can also be viewed as way to compare base pair probability matrices. PMmulti is a wrapper program that does progressive multiple alignments by repeatedly calling pmcomp | yes | yes | no | sourcecode, webserver | [52] | |
RNAG | A Gibbs sampling method to determine a conserved structure and the structural alignment. | any | yes | yes | no | sourcecode | [53] |
R-COFFEE | uses RNAlpfold to compute the secondary structure of the provided sequences. A modified version of T-Coffee is then used to compute the multiple sequence alignment having the best agreement with the sequences and the structures. R-Coffee can be combined with any existing sequence alignment method. | any | yes | yes | no | sourcecode, webserver | [54][55] |
TurboFold | This algorithm predicts conserved structures in any number of sequences. It uses probabilistic alignment and partition functions to map conserved pairs between sequences, and then iterates the partition functions to improve structure prediction accuracy | any | no | yes | yes | sourcecode | [56][57] |
RNA123 | The structure based sequence alignment (SBSA) algorithm within RNA123 utilizes a novel suboptimal version of the Needleman-Wunsch global sequence alignment method that fully accounts for secondary structure in the template and query. It also utilizes two separate substitution matrices that are optimized for RNA helices and single stranded regions. The SBSA algorithm provides >90% accurate sequence alignments even for structures as large as bacterial 23S rRNA (~2800 nts). | any | yes | yes | yes | webserver | |
RNAalifold | Folds precomputed alignments using a combination of free-energy and a covariation measures. Ships with the Vienna package. | any | input | yes | no | homepage | [10][58] |
RNAcast | enumerates the near-optimal abstract shape space, and predicts as the consensus an abstract shape common to all sequences, and for each sequence, the thermodynamically best structure which has this abstract shape. | any | no | yes | no | sourcecode, webserver | [59] |
RNAforester | Compare and align RNA secondary structures via a "forest alignment" approach. | any | yes | input | no | sourcecode, webserver | [60][61] |
RNAmine | Frequent stem pattern miner from unaligned RNA sequences is a software tool to extract the structural motifs from a set of RNA sequences. | any | no | yes | no | webserver | [62] |
RNASampler | A probabilistic sampling approach that combines intrasequence base pairing probabilities with intersequence base alignment probabilities. This is used to sample possible stems for each sequence and compare these stems between all pairs of sequences to predict a consensus structure for two sequences. The method is extended to predict the common structure conserved among multiple sequences by using a consistency-based score that incorporates information from all the pairwise structural alignments. | any | yes | yes | yes | sourcecode | [63] |
SCARNA | Stem Candidate Aligner for RNA (Scarna) is a fast, convenient tool for structural alignment of a pair of RNA sequences. It aligns two RNA sequences and calculates the similarities of them, based on the estimated common secondary structures. It works even for pseudoknotted secondary structures. | 2 | yes | yes | no | webserver | [64] |
SimulFold | simultaneously inferring RNA structures including pseudoknots, alignments, and trees using a Bayesian MCMC framework. | any | yes | yes | yes | sourcecode | [65] |
Stemloc | a program for pairwise RNA structural alignment based on probabilistic models of RNA structure known as Pair stochastic context-free grammars. | any | yes | yes | no | sourcecode | [66] |
StrAl | an alignment tool designed to provide multiple alignments of non-coding RNAs following a fast progressive strategy. It combines the thermodynamic base pairing information derived from RNAfold calculations in the form of base pairing probability vectors with the information of the primary sequence. | yes | no | no | sourcecode, webserver | [67] | |
TFold | A tool for predicting non-coding RNA secondary structures including pseudoknots. It takes in input an alignment of RNA sequences and returns the predicted secondary structure(s).It combines criteria of stability, conservation and covariation in order to search for stems and pseudoknots. Users can change different parameters values, set (or not) some known stems (if there are) which are taken into account by the system, choose to get several possible structures or only one, search for pseudoknots or not, etc. | any | yes | yes | yes | webserver | [68] |
WAR | a webserver that makes it possible to simultaneously use a number of state of the art methods for performing multiple alignment and secondary structure prediction for noncoding RNA sequences. | yes | yes | no | webserver | [69] | |
Xrate | a program for analysis of multiple sequence alignments using phylogenetic grammars, that may be viewed as a flexible generalization of the "Pfold" program. | any | yes | yes | no | sourcecode | [70] |
|
Inter molecular interactions: RNA-RNA
Many ncRNAs function by binding to other RNAs. For example, miRNAs regulate protein coding gene expression by binding to 3' UTRs, small nucleolar RNAs guide post-transcriptional modifications by binding to rRNA, U4 spliceosomal RNA and U6 spliceosomal RNA bind to each other forming part of the spliceosome and many small bacterial RNAs regulate gene expression by antisense interactions E.g. GcvB, OxyS and RyhB.
Name | Description | Intra-molecular structure | Comparative | Link | References |
---|---|---|---|---|---|
GUUGle | A utility for fast determination of RNA-RNA matches with perfect hybridization via A-U, C-G, and G-U base pairing. | no | no | webserver | [71] |
IntaRNA | Efficient target prediction incorporating the accessibility of target sites | yes | no | sourcecode webserver | [72][73][74] |
NUPACK | Computes the full unpseudoknotted partition function of interacting strands in dilute solution. Calculates the concentrations, mfes, and base-pairing probabilities of the ordered complexes below a certain complexity. Also computes the partition function and basepairing of single strands including a class of pseudoknotted structures. Also enables design of ordered complexes. | yes | no | NUPACK | [75] |
OligoWalk/RNAstructure | Predicts bimolecular secondary structures with and without intramolecular structure. Also predicts the hybridization affinity of a short nucleic acid to an RNA target. | yes | no | [1] | [76] |
piRNA | calculates the partition function and thermodynamics of RNA-RNA interactions. It considers all possible joint secondary structure of two interacting nucleic acids that do not contain pseudoknots, interaction pseudoknots, or zigzags. | yes | no | linuxbinary | [77] |
RNAaliduplex | Based upon RNAduplex with bonuses for covarying sites | no | yes | sourcecode | [10] |
RNAcofold | works much like RNAfold, but allows to specify two RNA sequences which are then allowed to form a dimer structure. | yes | no | sourcecode | [10][78] |
RNAduplex | computes optimal and suboptimal secondary structures for hybridization. The calculation is simplified by allowing only inter-molecular base pairs. | no | no | sourcecode | [10] |
RNAhybrid | a tool for finding the minimum free energy hybridisation of a long and a short RNA. | no | no | sourcecode, webserver | [79][80] |
RNAup | calculates the thermodynamics of RNA-RNA interactions. RNA-RNA binding is decomposed into two stages. (1) First the probability that a sequence interval (e.g. a binding site) remains unpaired is computed. (2) Then the binding energy given that the binding site is unpaired is calculated as the optimum over all possible types of bindings. | yes | no | sourcecode | [10][81] |
Inter molecular interactions: MicroRNA:UTR
MicroRNAs regulate protein coding gene expression by binding to 3' UTRs, there are tools specifically designed for predicting these interactions. For an evaluation of target prediction methods on high-throughput experimental data see (Selbach et al., Nature 2008) [82] and (Alexiou et al., Bioinformatics 2009)[83]
Name | Description | Species Specific | Intra-molecular structure | Comparative | Link | References |
---|---|---|---|---|---|---|
Diana-microT | DIANA-microT 3.0 is an algorithm based on several parameters calculated individually for each microRNA and it combines conserved and non-conserved microRNA recognition elements into a final prediction score. | human, mouse | no | yes | webserver | [84] |
MicroTar | An animal miRNA target prediction tool based on miRNA-target complementarity and thermodynamic data. | no | no | no | sourcecode | [85] |
miTarget | microRNA target gene prediction using a support vector machine. | no | no | no | webserver | [86] |
PicTar | Combinatorial microRNA target predictions. | 8 vertebrates | no | yes | predictions | [87] |
PITA | Incorporates the role of target-site accessibility, as determined by base-pairing interactions within the mRNA, in microRNA target recognition. | no | yes | no | executable, webserver, predictions | [88] |
RNA22 | The first link (predictions) provides RNA22 predictions for all protein coding transcripts in human, mouse, roundworm, and fruit fly. It allows you to visualize the predictions within a cDNA map and also find transcripts where multiple miR's of interest target. The second web-site link (custom) first finds putative microRNA binding sites in the sequence of interest, then identifies the targeted microRNA. | no | no | no | predictions custom | [89] |
RNAhybrid | a tool for finding the minimum free energy hybridisation of a long and a short RNA. | no | no | no | sourcecode, webserver | [79][80] |
Sylamer | Sylamer is a method for finding significantly over or under-represented words in sequences according to a sorted gene list. Typically it is used to find significant enrichment or depletion of microRNA or siRNA seed sequences from microarray expression data. | no | no | no | sourcecode webserver | [90][91] |
TAREF | TAREF stands for TARget REFiner. It predicts microRNA targets on the basis of multiple feature information derived from the flanking regions of the predicted target sites where traditional structure prediction approach may not be successful to assess the openness. It also provides an option to use encoded pattern to refine filtering. | Yes | no | no | server/sourcecode | [92] |
p-TAREF | p-TAREF stands for plant TARget REFiner. It identifies plant microRNA targets on the basis of multiple feature information derived from the flanking regions of the predicted target sites where traditional structure prediction approach may not be successful to assess the openness. It also provides an option to use encoded pattern to refine filtering. It first time employed power of machine learning approach with scoring scheme through Support Vector Regression(SVR) while considering structural and alignment aspects of targeting in plants with plant specific models. p-TAREF has been implemented in concurrent architecture in server as well as standalone form, making it one of the very few available target identification tools able to run concurrently on simple desktops while performing huge transcriptome level analysis accurately and fast. Besides this, it also provides an option to experimentally validate the predicted targets, on the spot, using expression data, which has been integrated in its back-end, to draw confidence on prediction along with SVR score.p-TAREF performance benchmarking has been done extensively through different tests and compared with other plant miRNA target identification tools. p-TAREF was found better performing. | Yes | no | no | server/standalone | |
TargetScan | Predicts biological targets of miRNAs by searching for the presence of conserved 8mer and 7mer sites that match the seed region of each miRNA. Predictions are ranked using site number, site type, and site context, which includes factors that influence target-site accessibility. | vertebrates, flies, nematodes | evaluated indirectly | yes | sourcecode, webserver | [93][94][95][96] |
ncRNA gene prediction software
Name | Description | Number of sequences [Note 1] |
Alignment [Note 2] |
Structure [Note 3] |
Link | References |
---|---|---|---|---|---|---|
Alifoldz | Assessing a multiple sequence alignment for the existence of an unusual stable and conserved RNA secondary structure. | any | input | yes | sourcecode | [97] |
EvoFold | a comparative method for identifying functional RNA structures in multiple-sequence alignments. It is based on a probabilistic model-construction called a phylo-SCFG and exploits the characteristic differences of the substitution process in stem-pairing and unpaired regions to make its predictions. | any | input | yes | linuxbinary | [98] |
MSARi | heuristic search for statistically significant conservation of RNA secondary structure in deep multiple sequence alignments. | any | input | yes | sourcecode | [99] |
QRNA | This is the code from Elena Rivas that accompanies a submitted manuscript "Noncoding RNA gene detection using camparative sequence analysis". QRNA uses comparative genome sequence analysis to detect conserved RNA secondary structures, including both ncRNA genes and cis-regulatory RNA structures. | 2 | input | yes | sourcecode | [100][101] |
RNAz | program for predicting structurally conserved and thermodynamic stable RNA secondary structures in multiple sequence alignments. It can be used in genome wide screens to detect functional RNA structures, as found in noncoding RNAs and cis-acting regulatory elements of mRNAs. | any | input | yes | sourcecode, webserver RNAz 2 | [102][103][104] |
Xrate | a program for analysis of multiple sequence alignments using phylogenetic grammars, that may be viewed as a flexible generalization of the "Evofold" program. | any | yes | yes | sourcecode | [70] |
|
Family specific gene prediction software
Name | Description | Family | Link | References |
---|---|---|---|---|
ARAGORN | ARAGORN detects tRNA and tmRNA in nucleotide sequences. | tRNA tmRNA | webserver source | [105] |
miRNAminer | Given a search query, candidate homologs are identified using BLAST search and then tested for their known miRNA properties, such as secondary structure, energy, alignment and conservation, in order to assess their fidelity. | MicroRNA | webserver | [106] |
RISCbinder | Prediction of guide strand of microRNAs. | Mature miRNA | webserver | [107] |
RNAmicro | A SVM-based approach that, in conjunction with a non-stringent filter for consensus secondary structures, is capable of recognizing microRNA precursors in multiple sequence alignments. | MicroRNA | homepage | [108] |
RNAmmer | RNAmmer uses HMMER to annotate rRNA genes in genome sequences. Profiles were built using alignments from the European ribosomal RNA database[109] and the 5S Ribosomal RNA Database.[110] | rRNA | webserver source | [111] |
SnoReport | Uses a combination of RNA secondary structure prediction and machine learning that is designed to recognize the two major classes of snoRNAs, box C/D and box H/ACA snoRNAs, among ncRNA candidate sequences. | snoRNA | sourcecode | [112] |
SnoScan | Search for C/D box methylation guide snoRNA genes in a genomic sequence. | C/D box snoRNA | sourcecode, webserver | [113][114] |
snoSeeker | snoSeeker includes two snoRNA-searching programs, CDseeker and ACAseeker, specific to the detection of C/D snoRNAs and H/ACA snoRNAs, respectively. snoSeeker has been used to scan four human–mammal whole-genome alignment (WGA) sequences and identified 54 novel candidates including 26 orphan candidates as well as 266 known snoRNA genes. | snoRNA | webserver,stand-alone | [115] |
tRNAscan-SE | a program for the detection of transfer RNA genes in genomic sequence. | tRNA | sourcecode, webserver | [114][116] |
miRNAFold | A fast ab initio software for searching for microRNA precursors in genomes. | microRNA | webserver | [117] |
RNA homology search software
Name | Description | Link | References |
---|---|---|---|
ERPIN | "Easy RNA Profile IdentificatioN" is an RNA motif search program reads a sequence alignement and secondary structure, and automatically infers a statistical "secondary structure profile" (SSP). An original Dynamic Programming algorithm then matches this SSP onto any target database, finding solutions and their associated scores. | sourcecode webserver | [118][119][120] |
Infernal | "INFERence of RNA ALignment" is for searching DNA sequence databases for RNA structure and sequence similarities. It is an implementation of a special case of profile stochastic context-free grammars called covariance models (CMs). | sourcecode | [121][122][123] |
PHMMTS | "pair hidden Markov models on tree structures" is an extension of pair hidden Markov models defined on alignments of trees. | sourcecode, webserver | [124] |
RaveNnA | A slow and rigorous or fast and heuristic sequence-based filter for covariance models. | sourcecode | [125][126] |
RSEARCH | Takes a single RNA sequence with its secondary structure and utilizes a local alignment algorithm to search a database for homologous RNAs. | sourcecode | [127] |
Structator | Ultra fast software for searching for RNA structural motifs employing an innovative index-based bidirectional matching algorithm combined with a new fast fragment chaining strategy. | sourcecode | [128] |
Benchmarks
Name | Description | Structure[Note 1] | Alignment[Note 2] | Phylogeny | Links | References |
---|---|---|---|---|---|---|
BRalibase I | A comprehensive comparison of comparative RNA structure prediction approaches | yes | no | no | data | [129] |
BRalibase II | A benchmark of multiple sequence alignment programs upon structural RNAs | no | yes | no | data | [130] |
BRalibase 2.1 | A benchmark of multiple sequence alignment programs upon structural RNAs | no | yes | no | data | [131] |
BRalibase III | A critical assessment of the performance of homology search methods on noncoding RNA | no | yes | no | data | [132] |
CompaRNA | An independent comparison of single-sequence RNA secondary structure prediction programs | yes | no | no | CompaRNA | |
|
Alignment viewers/editors
Name | Description | Alignment[Note 1] | Structure[Note 2] | Link | References |
---|---|---|---|---|---|
4sale | A tool for Synchronous RNA Sequence and Secondary Structure Alignment and Editing | yes | yes | sourcecode | [133] |
Colorstock, SScolor, Raton | Colorstock, a command-line script using ANSI terminal color; SScolor, a Perl script that generates static HTML pages; and Raton, an AJAX web application generating dynamic HTML. Each tool can be used to color RNA alignments by secondary structure and to visually highlight compensatory mutations in stems. | yes | yes | sourcecode | [134] |
Integrated Genome Browser (IGB) | a multiple alignment viewer written in Java. | yes | no | sourcecode | [135] |
Jalview | a multiple alignment editor written in Java. | yes | no | sourcecode | [136][137] |
RALEE | a major mode for the Emacs text editor. It provides functionality to aid the viewing and editing of multiple sequence alignments of structured RNAs. | yes | yes | sourcecode | [138] |
SARSE | A graphical sequence editor for working with structural alignments of RNA. | yes | yes | sourcecode | [139] |
|
Inverse Folding/RNA design
Name | Description | Link | References |
---|---|---|---|
ETeRNA | An RNA folding game that challenges players to come up with sequences that fold into a target RNA structure. The best sequences for a given puzzle are synthesized and their structures are probed through chemical mapping. The sequences are then scored by the data's agreement to the target structure and feedback is provided to the players. | home page | -- |
NUPACK | Although NUPACK can be used to get useful statistics and properties of an RNA's structure as mentioned above, it's main goal is design of new sequences that fold into a desired structure. | home page | [75] |
RNAInverse | The ViennaRNA package provides RNAInverse, an algorithm for designing sequences with desired structure. | help page | [140] |
Secondary structure viewers/editors
Name | Description | Link | References |
---|---|---|---|
PseudoViewer | Automatically visualizing RNA pseudoknot structures as planar graphs. | webapp/binary | [141][142][143][144] |
RNA Movies | browse sequential paths through RNA secondary structure landscapes | sourcecode | [145][146] |
RNA2D3D | a program for generating, viewing, and comparing 3-dimensional models of RNA | binary | [147] |
RNAstructure | RNAstructure has a viewer for structures in ct files. It can also compare predicted structures using the circleplot program. Structures can be output as postscript files. | sourcecode | [148] |
RNAView/RnamlView | Use RNAView to automatically identify and classify the types of base pairs that are formed in nucleic acid structures. Use RnamlView to arrange RNA structures. | sourcecode | [149] |
VARNA | a tool for the automated drawing, visualization and annotation of the secondary structure of RNA, designed as a companion software for web servers and databases | sourcecode | [150] |
See also
References
- ^ Michiaki Hamada, Hisanori Kiryu, Kengo Sato, Toutai Mituyama, Kiyoshi Asai (2009). "Predictions of RNA secondary structure using generalized centroid estimators". Bioinformatics. 25 (4): 465–473. doi:10.1093/bioinformatics/btn601. PMID 19095700.
{{cite journal}}
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