Cytoplasmic hybrid

"Cybrid" redirects here. For other uses, see Cybrid (disambiguation).

Animal view of different embryos developing in Xenopus laevis eggs: haploid [laevis]x laevis (middle) and [laevis]x tropicalis cybrid (bottom) embryos cleave and begin gastrulation synchroneously, about 50 minutes after diploid laevis x laevis (top) embryos. A star was added to the right of embryos at the onset of gastrulation (stage 10), when embryo-wide cellular movements begin.

A cytoplasmic hybrid (or cybrid, a portmanteau of the two words) is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast. Cytoplasts are enucleated cells. This enucleation can be effected by simultaneous application of centrifugal force and treatment of the cell with an agent that disrupts the cytoskeleton. A special case of cybrid formation involves the use of rho-zero cells as the whole cell partner in the hippocampus of the brain. Rho-zero cells are cells which have been depleted of their own mitochondrial DNA by prolonged incubation with ethidium bromide, a chemical which inhibits mitochondrial DNA replication. The rho-zero cells do retain mitochondria and can grow in rich culture medium with certain supplements. They do retain their own nuclear genome. A cybrid is then a hybrid cell which combines the nuclear genome from one source with the mitochondrial genome from another source. Using this powerful tool, it is possible to dissociate the genetic contribution of the mitochondrial genome from that of the nuclear genome.

Cybrids are valuable in mitochondrial research and have been used to provide suggestive evidence of mitochondrial involvement in Alzheimer's disease, Parkinson's disease, and other conditions.