Zbyszek Darzynkiewicz
 | This article, Zbyszek Darzynkiewicz, has recently been created via the Articles for creation process. Please check to see if the reviewer has accidentally left this template after accepting the draft and take appropriate action as necessary.
Reviewer tools: Inform author |
Zbigniew (Dzierzykraj) Darzynkiewicz (born May 12, 1936, Dzisna, Wilno district, Poland; currently Vilnius, Lituania, USA naturalized, 1976) is a scientist focusing his interest on cell biology and cancer research.
Education, Positions
- Bulleted list item
MD (with the highest honors) 1960, Medical University of Warsaw, Poland. Doctor of Medical Sciences (PhD) 1966, Medical University of Warsaw.
- Bulleted list item
1965-66 - Research Associate, Department of Pharmacology, SUNY at Buffalo, N.Y.
- Bulleted list item
1968-69, Visiting ScientistLaboratory of Cell Research, Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden.
- Bulleted list item
1988-90, Member, Memorial Sloan Kettering Institute, New York, NY; Professor of Cell Biology and Genetics, Cornell University, Graduate School of Medical Sciences. Sciences, New York, N.Y.
- Bulleted list item
1998-90 Director, Flow Cytometry Core Facility Network, Memorial Sloan-Kettering Cancer Center. Center, New York, N.Y.
- Bulleted list item
1990-pres. Professor of Medicine, New York Medical College, Valhalla, N.Y.; 1990-pres. Director, Brander Cancer Research Institute (formerly: Cancer Research Institute at NYMC)
Research highlights:
1. Developed the flow cytometric methodology to differentially stain DNA versus DNA with acridine orange. This methodology was applied to distinguish the non-cycling (resting; G0 cells> from their cycling counterparts such as peripheral lymphocytes from the mitogenically stimulated blast cells[1]
2. Developed the flow cytometric methodology to identify apoptotic cells based on labeling DNA strand breaks with exogenous terminal transferase (the TUNEL assay)[2][3]
3. Developed the flow cytometric methodology to detect DNA denaturation in situ based on use of the metachromatic properties of acridine orange [4].
4 Used this methodology to detect abnormality of chromatin structure of infertile sperm cells[5][6] This methodology is currently being used as one of the key methods to assess male fertility in animal and human clinic [7]
5. First reported that abnormal, infertile human sperm cells have extensive DNA fragmentation being detected by the TUNEL assay [8] This methodology now serves also as the means of identification of abnormal, infertile sperm cells.
- ^ Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1976) Lymphocyte stimulation: A rapid multiparameter analysis. Proc Natl Acad Sci USA 73:2881-2884, PMID: 822422
- ^ Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, PMID: 1333943
- ^ Gorczyca W, Gong J, Darzynkiewicz. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945-1951 PMID: 8467513 Online ISSN 1538-7445.
- ^ Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1975) Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 90:411-428, PMID: 46199
- ^ Evenson DP, Darzynkiewicz Z, Melamed MR. (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131-1133. PMID: 7444440
- ^ US patent Flow Cytometry-Fluorescence Measurements for Characterizing Sperm. U.S. Patent No. 4,559,309, issued Dec. 17, 1985
- ^ DP Evenson, LK Jost, D Marshall, MJ Zinaman, E Clegg, K Purvis, P De Angelis, OP Claussen. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction 19994;4/1/1039-1049 https://doi.org/10.1093/humrep/14.4.1039
- ^ Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. (1993) Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 207:202-205. PMID: 8391465 DOI: 10.1006/excr.1993.1182