Zbyszek Darzynkiewicz
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Zbigniew (Dzierzykraj) Darzynkiewicz (born May 12, 1936, Dzisna, Wilno district, Poland; currently Vilnius, Lituania, USA naturalized, 1976) is a scientist focusing his interest on cell biology and cancer research.
Education, positions
1960, MD (with the highest honors) Medical University of Warsaw, Poland. 1966, Doctor of Medical Sciences (PhD) Medical University of Warsaw. 1965-66, Research Associate, Department of Pharmacology, SUNY at Buffalo, N.Y. 1968-69, Visiting ScientistLaboratory of Cell Research, Medical Nobel Institute, Karolinska Institute, Stockholm, Sweden. 1988-90, Member, Memorial Sloan Kettering Institute, New York, NY, & Professor of Cell Biology and Genetics, Cornell University, Graduate School of Medical Sciences. Sciences, New York, N.Y. 1998-90, Director, Flow Cytometry Core Facility Network, Memorial Sloan-Kettering Cancer Center. Center, New York, N.Y. 1990-pres. Professor of Pathology, Professor of Medicine and Professor of Microbiology/Immunology, New York Medical College, Valhalla, N.Y. 1990-pres. Director, Brander Cancer Research Institute (formerly: Cancer Research Institute at NYMC)
Honors, Awards
1986-87 President, The Cell Kinetics Society. 1986-99 Member, Advisory Board, The Alfred Jurzykowski Foundation, New York, N.Y. 1987 MERIT Award Research Grant from NCI (R37-CA23296) "Effects of New Anticancer Drugs on the Cell Cycle" (Principal Investigator). 1992-94 President, the International Society for Advancement of Cytometry. Program Chairman, 17th ISAC Congress (Lake Placid, NY. 1994). 1995 Honorary Member, Cytometry Society of Japan.
Research highlights:
1. Developed the flow cytometric methodology to differentially stain DNA versus DNA with acridine orange. This methodology was applied to distinguish the non-cycling (resting; G0 cells> from their cycling counterparts, such as peripheral blood lymphocytes from mitogenically stimulated lymphocytes[1]
2. Developed the flow cytometric methodology to identify apoptotic cells based on labeling DNA strand breaks with exogenous terminal deoxynucleotidyl transferase (the TUNEL assay)[2][3]
3. Developed the flow cytometric methodology to detect DNA denaturation in situ based on use of the metachromatic properties of acridine orange [4].
4 Used this methodology to detect abnormality of chromatin structure of infertile sperm cells[5][6] This methodology is currently being used as one of the key methods to assess male fertility in animal and human clinic [7]
5. First reported that abnormal, infertile human sperm cells have extensive DNA fragmentation being detected by the TUNEL assay [8] This methodology now serves also as the means of identification of abnormal, infertile sperm cells.
6. Developed still another assay to identify apoptotic cells by flow cytometry, based on the use Fluorochrome Labeled Inhibitors of Caspases (the FLICA assay)[9]
7. Developed the methodology to detect potential cancerogenic properties of tobacco smoke by measuring the smoke-induced DNA damage reported by phosphorylation of histone H2AX and activation of ATM in individual cells by flow cytometry[10][11]
References
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- ^ Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1976) Lymphocyte stimulation: A rapid multiparameter analysis. Proc Natl Acad Sci USA 73:2881-2884, PMID: 822422
- ^ Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, PMID: 1333943
- ^ Gorczyca W, Gong J, Darzynkiewicz. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945-1951 PMID: 8467513 Online ISSN 1538-7445.
- ^ Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. (1975) Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry. Exp Cell Res 90:411-428, PMID: 46199
- ^ Evenson DP, Darzynkiewicz Z, Melamed MR. (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131-1133. PMID: 7444440
- ^ US patent Flow Cytometry-Fluorescence Measurements for Characterizing Sperm. U.S. Patent No. 4,559,309, issued Dec. 17, 1985
- ^ DP Evenson, LK Jost, D Marshall, MJ Zinaman, E Clegg, K Purvis, P De Angelis, OP Claussen. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction 19994;4/1/1039-1049 https://doi.org/10.1093/humrep/14.4.1039
- ^ Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. (1993) Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 207:202-205. PMID: 8391465 DOI: 10.1006/excr.1993.1182
- ^ Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. (2000) Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res 259: 308-313. PMID: 10942603 DOI: 10.1006/excr.2000.4955
- ^ Approaches to Identify Less Harmful Tobacco and Tobacco Products US patent No 7,662,565, issued February 16, 2010.
- ^ Albino AP, Huang X, Jorgensen E, Yang J, Gietl D, Traganos F, Darzynkiewicz Z. Induction of H2AX phosphorylation in pulmonary cells by tobacco smoke: a new assay for carcinogens.Cell Cycle. 2004 Aug;3(8):1062-8.PMID: 15254392