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Y RNA

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Y RNA
Predicted secondary structure and sequence conservation of Y_RNA
Identifiers
SymbolY_RNA
Alt. SymbolsY1; Y2; Y3; Y5
RfamRF00019
Other data
RNA typeGene
Domain(s)Eukaryota
SOSO:0000405
PDB structuresPDBe

Y RNAs are small non-coding RNAs. They are components of the Ro60 ribonucleoprotein particle[1] which is a target of autoimmune antibodies in patients with systemic lupus erythematosus.[2] They are also necessary for DNA replication through interactions with chromatin and initiation proteins.[3][4]

Structure

These small RNAs are predicted to fold into a conserved stem formed by the RNA's 3' and 5' ends and characterized by a single bulged cytosine, which are the known requirements for Ro binding.[5][6][7]

Function

Two functions have been described for Y RNAs in the literature: As a repressor of Ro60, and as an initiation factor for DNA replication. Mutant human Y RNAs lacking the conserved binding site for Ro60 protein still support DNA replication,[3] indicating that binding to Ro protein and promoting DNA replication are two separable functions of Y RNAs. Although Y RNA-derived small RNAs are similar in size to microRNAs, it has been shown that these Y RNA fragments are not involved in the microRNA pathway.[8]

The Ro autoantigen protein (white) binds the end of a double-stranded Y RNA (red) and a single stranded RNA (blue). (PDB: 1YVP​).[5]

Ro60 Inhibition

In its free state, Ro binds to a variety of misfolded RNAs including misfolded 5S rRNAs, and is thought to act as some sort of quality control mechanism.[9] Crystal structures of Ro complexed either with Y RNA or another RNA showed that Ro binds single-stranded 3' ends of RNAs relatively nonspecifically, whereas Y RNA binds specifically at a second site that regulates access of other RNAs.[5] In Deinococcus, free Ro has also been shown to function in 23S rRNA maturation.[10] In Deinococcus, mutants lacking Y RNA are viable, and Y RNA appears to be unstable except when complexed with Ro.[10]

DNA replication initiation

Human Y RNAs are functionally required for DNA replication.[3] Biochemical fractionation and reconstitution experiments have established a functional requirement of human Y RNAs for chromosomal DNA replication in isolated vertebrate cell nuclei in vitro[3] and specific degradation of human Y RNAs inhibits DNA replication in vitro, and in intact cells in vivo.[3] Y RNA function is thought to be mediated via interactions with chromatin and initiation proteins (including the origin recognition complex)[4]

In human pathology

Y RNAs are overexpressed in some human tumours and are required for cell proliferation[11] and small, microRNA-sized breakdown products may be involved in autoimmunity and other pathological conditions.[12] Recent work has demonstrated that Y RNAs are modified at their 3' end by the non-canonical poly(A) polymerase PAPD5, and the short oligo(A) tail added by PAPD5 is a marker for 3' end processing by the ribonuclease PARN/EXOSC10 or for degradation by the exonuclease DIS3L.[13] Since PARN deficiency causes a severe form of the bone marrow disease Dyskeratosis Congenita as well as pulmonary fibrosis,[14][15] it is possible that defects in Y RNA processing contribute to the severe pathology observed in these patients.

Species distribution

Presumptive Y RNA and Ro protein homologs have been found in eukaryotes and bacteria.[6][16]

Humans

Humans appear to have four Y RNAs, named hY1, hY3, hY4 and hY5[16] and also a large number of pseudogenes.

C. elegans

Caenorhabditis elegans has one, named CeY RNA and a large number of sbRNAs that are postulated to also be Y RNA homologues.[17][18]

D. radiodurans

The radiation-resistant bacterium Deinococcus radiodurans encodes a homolog of Ro called rsr ("Ro sixty related"), and at least four small RNAs accumulate in Deinococcus under conditions where rsr expression is induced (UV irradiation); one of these RNAs appears to be a Y RNA homolog.[19] In Deinococcus radiodurans Rsr is tethered via Y RNA to the exoribonuclease PNPase and channels single-stranded RNA into the PNPase cavity. Rsr and Y RNA enhance degradation of structured RNAs by PNPase. This role could be conserved, as Rsr and ncRNAs called YrlA and YrlB (Y RNA like) also associate with PNPase in an evolutionary distant bacterium Salmonella Typhimurium.[20]

References

  1. ^ Y RNAs: recent developments. Adam E. Hall, Carly Turnbull, Tamas Dalmay. Biomolecular Concepts. 2013 January; 4(2):103-110. doi:10.1515/bmc-2012-0050.
  2. ^ Lerner, MR; Boyle JA; Hardin JA; Steitz JA (1981). "Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus". Science. 211 (4480): 400–402. doi:10.1126/science.6164096. PMID 6164096.
  3. ^ a b c d e Christov CP, Gardiner TJ, Szüts D, Krude T (2006). "Functional Requirement of Noncoding Y RNAs for Human Chromosomal DNA Replication". Mol. Cell. Biol. 26 (18): 6993–7004. doi:10.1128/MCB.01060-06. PMC 1592862. PMID 16943439.
  4. ^ a b Zhang, AT; Langley, AR; Christov, CP; Kheir, E; Shafee, T; Gardiner, TJ; Krude, T (Jun 15, 2011). "Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication". Journal of Cell Science. 124 (Pt 12): 2058–69. doi:10.1242/jcs.086561. PMC 3104036. PMID 21610089.
  5. ^ a b c Stein, AJ; Fuchs G; Fu C; Wolin SL; Reinisch KM (2005). "Structural insights into RNA quality control: The Ro autoantigen binds misfolded RNAs via its central cavity". Cell. 121 (4): 529–537. doi:10.1016/j.cell.2005.03.009. PMC 1769319. PMID 15907467.
  6. ^ a b Teunissen SW, Kruithof MJ, Farris AD, Harley JB, Venrooij WJ, Pruijn GJ (2000). "Conserved features of Y RNAs: a comparison of experimentally derived secondary structures". Nucleic Acids Res. 28 (2): 610–9. doi:10.1093/nar/28.2.610. PMC 102524. PMID 10606662.
  7. ^ Green, CD; Long KS; Shi H; Wolin SL (1998). "Binding of the 60-kDa Ro autoantigen to Y RNAs: evidence for recognition in the major groove of a conserved helix". RNA. 4 (7): 750–765. doi:10.1017/S1355838298971667. PMC 1369656. PMID 9671049.
  8. ^ Biogenesis of Y RNA-derived small RNAs is independent of the microRNA pathway. Francisco Esteban Nicolas, Adam E. Hall, Tibor Csorba, Carly Turnbull, Tamas Dalmay. FEBS Letters. 2012 April; 586(8):1226-1230. doi:10.1016/j.febslet.2012.03.026.
  9. ^ Reinisch, KM; Wolin SL (2007). "Emerging themes in non-coding RNA quality control". Current Opinion in Structural Biology. 17 (2): 209–214. doi:10.1016/j.sbi.2007.03.012. PMID 17395456.
  10. ^ a b Chen X, Wurtmann EJ, Van Batavia J, Zybailov B, Washburn MP, Wolin SL (2007). "An ortholog of the Ro autoantigen functions in 23S rRNA maturation in D. radiodurans". Genes Dev. 21 (11): 1328–39. doi:10.1101/gad.1548207. PMC 1877746. PMID 17510283.
  11. ^ Christov CP, Trivier E, Krude T (March 2008). "Noncoding human Y RNAs are overexpressed in tumours and required for cell proliferation". Br. J. Cancer. 98 (5): 981–8. doi:10.1038/sj.bjc.6604254. PMC 2266855. PMID 18283318.
  12. ^ Are the Ro RNP-associated Y RNAs concealing microRNAs? Y RNA-derived miRNAs may be involved in autoimmunity. Verhagen AP, Pruijn GJ. Bioessays. 2011 Sep;33(9):674-82. doi:10.1002/bies.201100048.
  13. ^ Shukla, Siddharth; Parker, Roy (2017-09-26). "PARN Modulates Y RNA Stability and Its 3′-End Formation". Molecular and Cellular Biology. 37 (20). doi:10.1128/MCB.00264-17. ISSN 0270-7306. PMC 5615183. PMID 28760775.
  14. ^ Stuart, Bridget D.; Choi, Jungmin; Zaidi, Samir; Xing, Chao; Holohan, Brody; Chen, Rui; Choi, Mihwa; Dharwadkar, Pooja; Torres, Fernando (May 2015). "Exome Sequencing Links Mutations in PARN and RTEL1 with Familial Pulmonary Fibrosis and Telomere Shortening". Nature genetics. 47 (5): 512–517. doi:10.1038/ng.3278. ISSN 1061-4036. PMC 4414891. PMID 25848748.
  15. ^ Tummala, Hemanth; Walne, Amanda; Collopy, Laura; Cardoso, Shirleny; de la Fuente, Josu; Lawson, Sarah; Powell, James; Cooper, Nicola; Foster, Alison (2015-05-01). "Poly(A)-specific ribonuclease deficiency impacts telomere biology and causes dyskeratosis congenita". The Journal of Clinical Investigation. 125 (5): 2151–2160. doi:10.1172/JCI78963. ISSN 0021-9738. PMC 4463202. PMID 25893599.
  16. ^ a b Perreault J, Perreault JP, Boire G (2007). "Ro-associated Y RNAs in metazoans: evolution and diversification". Mol. Biol. Evol. 24 (8): 1678–89. doi:10.1093/molbev/msm084. PMID 17470436.
  17. ^ Van Horn, DJ; Eisenberg D; O'Brien CA; Wolin SL (1995). "Caenorhabditis elegans embryos contain only one major species of Ro RNP". RNA. 1 (3): 293–303. PMC 1369082. PMID 7489501.
  18. ^ Boria I, Gruber AR, Tanzer A, et al. (March 2010). "Nematode sbRNAs: Homologs of Vertebrate Y RNAs". J Mol Evol. 70 (4): 346–58. doi:10.1007/s00239-010-9332-4. PMID 20349053.
  19. ^ Chen, X; Quinn AM; Wolin SL (2000). "Ro ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation". Genes Dev. 14 (7): 777–782. doi:10.1101/gad.14.7.777. PMC 316496. PMID 10766734.
  20. ^ Chen, X.; Taylor, D. W.; Fowler, C. C.; Galan, J. E.; Wang, H.W.; Wolin, S. L. (2013). "An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA". Cell. 153 (1): 166–77. doi:10.1016/j.cell.2013.02.037. PMC 3646564. PMID 23540697.
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