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KIAA0895 is a gene found in Homo sapiens. The gene encodes a protein commonly known as the KIAA0895 protein. It's aliases include hypothetical protein LOC23366, OTTHUMP00000206979, OTTHUMP00000206980, 9530077C05Rik, 1110003N12Rik, Kiaa0895, and mKIAA0895.[1] Research into the KIAA proteins has shown that they are similar to known genes with functions related to cell signaling/communication, cell structure/motility and nucleic acid management.[2]
Gene
The KIAA0895 gene is located at 7p14.2.
It can be transcribed into 15 transcript variants, which in turn can produce 13 different isoforms of the protein.[3]
The genomic DNA is 65,976 base pairs long,[4] while the longest mRNA that it produces is 4463 bases long.
Gene Neighborhood
KIAA0895 is surrounded by the following genes on chromosome 7:[4]
Transcript
There are ten different isoforms for KIAA0895.[5]
- NP_001093895.1
- EAW94064.1
- NP_056129.2
- NP_001186636.1
- NP_001186635.1
- XP_005249746.1
- EAW94065.1
- XP_024302470.1
- NP_001186637.1
- NP_001287885.1
A theoretical stem-loop formation for the 5' UTR region which shows that there is a lack of conservation, meaning there is some precedence that these stems and loops are not used for translation regulation.[6] The ΔG value was -12.90 kcal/mol with three loops.[6]
A theoretical stem-loop formation was also found for the 3' UTR region which shows that there is conservation, meaning there is some precedence that they are used for translation regulation.[6] The ΔG value was -636.80 kcal/mol.[6]
Protein
The longest protein isoform that is produced by the KIAA0895 gene is termed LOC23366 isoform 1 and is 520 amino acids long.[7] The predicted molecular weight is 61kDa.[8] Additionally, the theoretical isoelectric point is 10.[8]
| Domain of unknown function 1704 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | DUF1704 | ||||||||
| Pfam | PF08014 | ||||||||
| InterPro | IPR012548 | ||||||||
| |||||||||
Regions
LOC23366 contains a protein domain of unknown function called DUF1704.[9] It also contains a region of low complexity from position 120 to position 150 in the protein,[10] and an arginine-rich area from position 12 to position 51.[11]
Amino Acid Composition
KIAA0895 is a lysine and arginine semi-enriched protein.[12] KIAA0895 is semi-enriched in positively charged lysine and arginine groups, and positively and negatively charged lysine, arginine, glutamic acid and aspartic acid groups.[12] However, KIAA0895 is depleted in non-polar alanine, glycine and proline groups.[12]
The charge distribution analysis shows that there are no negative or mixed charge clusters.[12] However, there is one positive charge cluster from amino acids 12 to 36.[12]
Post-translational Modification
KIAA0895 is predicted to undergo phosphorylation at several serines, threonines, and tyrosines throughout its structure.[13] Phosphorylation at these sites is a form of gene regulation. Phosphorylation results in a conformational change in the structure of many enzymes and receptors. This causes them to become activated or deactivated.
There are also predicted to be three N-linked glycosylation sites.[14] These occur at amino acids 142, 316, and 363. The sequences for these sites are NTS, NVS and NPT, respectfully. N-linked glycosylation has both intrinsic and extrinsic functions, regulating the migration patterns of cells.
Expression
KIAA0895 is most commonly found in the testis, however it also has a strong expression in the kidneys, adrenal glands, brain, ovary, skin and stomach.[5] It is also expressed in 20 other tissues at amounts smaller than 2.000 RPKM (Reads Per Kilobase of transcript per Million mapped reads).[5] Additionally, it is also found in cancerous tissues, most highly in cervical tumors and bladder carcinoma.
Homologs and Orthologs
KIAA0895 has over 228 orthologs.[5] Orthologs have been found in mammals and eukaryotes.[15] There are homologs in 9 species.[5] The full list of organisms in which homologs have been found is given below.
Paralogs
KIAA0895 has 7 paralogs in Homo sapiens.[15] The full list of paralogs are given below.
- Unnamed protein product
- Uncharacterized protein KIAA0895-like
- hCG28832, isoform CRA_a
- Hypothetical protein
- Unnamed protein product
- hCG38687, isoform CRA_a, partial
- hCG38687, isoform CRA_b
References
- ^ "GeneCards: KIAA0895 Gene". Retrieved 2011-04-24.
- ^ Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (December 1998). "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 5 (6): 355–64. doi:10.1093/dnares/5.6.355. PMID 10048485.
- ^ "NCBI AceView: KIAA0895". Retrieved 2011-04-24.
- ^ a b "Entrez Gene: KIAA0895". Retrieved 2011-04-24.
- ^ a b c d e "KIAA0895 KIAA0895 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2019-02-24.
- ^ a b c d "The Mfold Web Server | mfold.rit.albany.edu". unafold.rna.albany.edu. Retrieved 2019-04-17.
- ^ "NCBI Protein: LOC23366 isoform 1". Retrieved 2011-05-09.
- ^ a b "ExPASy - Compute pI/Mw tool". web.expasy.org. Retrieved 2019-04-17.
- ^ "Wellcome Trust Sanger Institute, Pfam". Archived from the original on 2014-06-17. Retrieved 2011-05-09.
{{cite web}}: Unknown parameter|dead-url=ignored (|url-status=suggested) (help) - ^ "MyHits Dotlet". Retrieved 2011-05-09.
- ^ "Uniprot". Retrieved 2011-05-09.
- ^ a b c d e "SAPS < Sequence Statistics < EMBL-EBI". www.ebi.ac.uk. Retrieved 2019-04-17.
- ^ "DTU Center for Biological Sciences, NetPhos". Retrieved 2011-05-09.
- ^ "DTU Center for Biological Sciences, NetNGlyc". Retrieved 2011-05-09.
- ^ a b "NCBI BLAST". Retrieved 2011-05-09.