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Entosis

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File:Entosis 1.jpg
Entosis: epithelial cell-in-cell formationA) Formation of cell-in-cell structure. B) DIC microscopy image of an entotic cell-in-cell structure of MCF10A cells with nuclei stained in blue C) Example of cell-in-cell structures in a breast carcinoma sample, stained with H&E.[1]

Entosis (from Greek ἐντός entos, "within" and -ωσις -osis, "disease") is the invasion of a living cell into another cell's cytoplasm.[2] The process was discovered by Overholtzer, et al. as reported in Cell.[3]

Entotic cells, also referred to as cell-in-cell structures, are triggered by loss of attachment to the extracellular matrix (ECM). This internalization of one cell by another is dependent on adherens junctions, and is driven by a Rho-dependent process, involving actin polymerization and myosin II activity in the internalized cell.[3] Adherens junctions bind cells together by linking cadherin transmembrane protein complexes of adjacent cells to the cytoskeleton. When certain cell types are detached from the ECM and have lost adhesion, the compaction force between neighboring cells can cause them to push into their neighbors, forming the trademark cell-in-cell structures.[4] Though cell-in-cell structures commonly refer to the interaction between two neighboring cells, entosis has been observed involving more than two cells. In the case of an entotic structure formed between three cells, the middle cell acts as both an internalizing and an outer host cell simultaneously.[4]

Aneuploidy, a condition in which non-disjunction gives rise to gametes with an abnormal number of chromosomes,[5] is one of the most prevalent phenotypes of human tumors. The underlying cause of aneuploidy remains highly debated; however, entosis is shown to perturb cytokinesis (cytoplasmic division) and trigger the formation of aneuploid cells.[2] This would be in line with past research, as cell-in-cell structures have been widely observed in the focused study of many human tumors, including lung, breast, and endometrial stromal carcinomas.[3]

A cell trapped by entosis is initially alive and can divide inside the cell that has enveloped it. On occasion, the entotic cell will be released by the host cell, but most internalized cells are eventually killed.[4] Normal cells can kill themselves via apoptosis, involving the programmed engulfment and phagocytic ingestion of a cell by another. Entosis differs greatly from apoptosis in that the entotic process exhibits behavior closely resembling cellular invasion rather than cellular engulfment.[3] Cancer cells adaptively avoid apoptosis, allowing them to live and multiply indefinitely, making it difficult to design drugs that effectively kill tumors. Therefore, entosis acts as a nonapoptotic cell death mechanism, and could possibly be a new way in which cancer cells can be killed.[4]

Genral mechanism

The mechanism of entotic cell cannibalism is a complex cell biology process. The process is initiated when epithelial cells form adherens junctions , this is followed by the generation og actomyosin-contractility. The combination of this processes drives cell engulfment by neighbor cell. After internalization, the inner cell is usually killed and digested by the outer cell. This process involves non-canonical autophagy, formation of lysosomes and nutrient recovery. In general, entosis greatly depends on cytoeskeletal structure changes and biophysical forces during the creation of cell-in-cell structures . Novel degradation and signaling pathways are employed during the inner cell killing and digestion process.[6]

Entosis in cancer

Entosis: the triggers and consequences of entosis in cancerEntosis can initialized by either aberrant mitosis of matrix deadhesion. Mitotic entosis can be induced by inhibition of Rap1 or Cdc42. RhoA activity is also important in both types of induced entosis. Entosis promotes death of the engulfed cell and benefits outer cell with nutrient gain. This could lead to both anti- and pro-tumorigenic effects depending on the outcome.[7]


Entosis has been found to be a different mechanism for cancer cells to form cell-in-cell structures at tumor sites. The entosis process in cancer cells is mediated via E-cadherin and P-cadherin. Since cadherins usually create homolytic cell to cell junctions, it is believed that the process mainly occurs between homologous cells. After cell-cell adhesions are mediated, the engulfed cells promote their own uptake into the neighbor cell. Additionally, they promote the ingestion process through actin polymerization and myosin contraction. The invading cell (outer cell) actomysin contraction is regulated by controllers or cell tension such as RhoA, furthermore they accumulate actin and myosin at the cell cortex which generates the mechanical tension that generates the cell-in-cell invasion mechanism. The entosis mechanism can potentially have substantial energetic implication in cancer cells compared to other mechanisms of cell death and engulfment. A crucial part of the process is the active involvement of invading cells, which does not happen in other forms of cell engulfment. This allows the mechanism to selectively target living cells, excluding dead cells or non-living material such as cell debris. After internalization, engulfed cells are killed by the host cell following the maturation of the entotic vacuole that encapsulates the entotic cell. The maturation of the entotic vacuole involves modification by autophagy pathway proteins, followed by lysosome fusion and inner cell dead and degradation inside the host cell. In this mechanism, autophagy pathway proteins play an important role by scavenging extracellular nutrients derived from the inner cell death. Internalized cells can also undergo alternative fates such as apoptosis or unharmed escape from host cell. In clinical cancer specimens, evidence of DNA fragmentation has been found suggesting that non-apoptotic cell death may be a common fate for entotic cells in human cancers.[8]

Videos

See also

References

  1. ^ "ScienceDirect". www.sciencedirect.com. doi:10.1016/j.bbamcr.2018.03.004. Retrieved 29 April 2019.
  2. ^ a b Janssen, Aniek; Rene H. Medema (1 March 2011). "Entosis: aneuploidy by invasion". Nature Cell Biology. 13: 199–201. doi:10.1038/ncb0311-199.
  3. ^ a b c d Overholtzer, Michael; Arnaud A. Mailleux; Ghassan Mouneimne; Guillaume Normand; Stuart J. Schnitt; Randall W. King; Edmund S. Cibas; Joan S. Brugge (30 November 2007). "A Nonapoptotic Cell Death Process, Entosis, that Occurs by Cell-in-Cell Invasion". Cell. 131 (5): 966–979. doi:10.1016/j.cell.2007.10.040. PMID 18045538.
  4. ^ a b c d Overholtzer, Michael. "An Interview with Michael Overholtzer". Memorial Sloan-Kettering Cancer Center. Retrieved 14 April 2012.
  5. ^ Alberts, Bruce (2010). Essential Cell Biology, 3rd Ed. New York, NY: Garland Science, Taylor & Franis Group, LLC. pp. 662–663. ISBN 978-0-8153-4129-1.
  6. ^ "ScienceDirect". www.sciencedirect.com. doi:10.1016/j.bbamcr.2018.03.004. Retrieved 29 April 2019.
  7. ^ Durgan, Joanne; Tseng, Yun-Yu; Hamann, Jens C; Domart, Marie-Charlotte; Collinson, Lucy; Hall, Alan; Overholtzer, Michael; Florey, Oliver (11 July 2017). Yap, Alpha (ed.). "Mitosis can drive cell cannibalism through entosis". eLife. 6: e27134. doi:10.7554/eLife.27134. ISSN 2050-084X.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  8. ^ Michael Overholtzer; Fais, Stefano (2018-12). "Cell-in-cell phenomena in cancer". Nature Reviews Cancer. 18 (12): 758–766. doi:10.1038/s41568-018-0073-9. ISSN 1474-1768. {{cite journal}}: Check date values in: |date= (help)

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