23S rRNA (guanine745-N1)-methyltransferase
| 23S rRNA (guanine745-N1)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.187 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
23S rRNA (guanine745-N1)-methyltransferase (EC 2.1.1.187, Rlma(I), Rlma1, 23S rRNA m1G745 methyltransferase, YebH, RlmAI methyltransferase, ribosomal RNA(m1G)-methylase, rRNA(m1G)methylase, RrmA, 23S rRNA:m1G745 methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (guanine745-N1)-methyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction
- S-adenosyl-L-methionine + guanine745 in 23S rRNA S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
The enzyme specifically methylates guanine745 at N1 in 23S rRNA.
References
- ^ Liu M, Novotny GW, Douthwaite S (November 2004). "Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase". RNA. 10 (11): 1713–20. doi:10.1261/rna.7820104. PMC 1370659. PMID 15388872.
- ^ Gustafsson C, Persson BC (January 1998). "Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant". Journal of Bacteriology. 180 (2): 359–65. doi:10.1128/JB.180.2.359-365.1998. PMC 106891. PMID 9440525.
- ^ Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT (March 2004). "Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site". Proceedings of the National Academy of Sciences of the United States of America. 101 (12): 4041–6. Bibcode:2004PNAS..101.4041D. doi:10.1073/pnas.0400189101. PMC 384692. PMID 14999102.
- ^ Hansen LH, Kirpekar F, Douthwaite S (July 2001). "Recognition of nucleotide G745 in 23 S ribosomal RNA by the rrmA methyltransferase". Journal of Molecular Biology. 310 (5): 1001–10. doi:10.1006/jmbi.2001.4836. PMID 11501991.
- ^ Liu M, Douthwaite S (April 2002). "Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes Gram-negative from Gram-positive bacteria". Molecular Microbiology. 44 (1): 195–204. doi:10.1046/j.1365-2958.2002.02866.x. PMID 11967079.
External links
- 23S+rRNA+(guanine745-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
